Gianni Ciofani

Summary

Affiliation: Scuola Superiore Sant'Anna
Country: Italy

Publications

  1. pmc Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
    G Ciofani
    Scuola Superiore Sant Anna, Pisa, Italy
    Int J Nanomedicine 5:285-98. 2010
  2. doi request reprint A simple approach to covalent functionalization of boron nitride nanotubes
    Gianni Ciofani
    Italian Institute of Technology, Smart Materials Lab, Center for MicroBioRobotics Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    J Colloid Interface Sci 374:308-14. 2012
  3. doi request reprint Hypergravity effects on myoblast proliferation and differentiation
    Gianni Ciofani
    Italian Institute of Technology, Center of MicroBioRobotics c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    J Biosci Bioeng 113:258-61. 2012
  4. pmc Pilot in vivo toxicological investigation of boron nitride nanotubes
    Gianni Ciofani
    Italian Institute of Technology, Center of Micro BioRobotics c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, Pontedera Pisa, Italy
    Int J Nanomedicine 7:19-24. 2012
  5. doi request reprint Preparation, characterization and in vitro testing of poly(lactic-co-glycolic) acid/barium titanate nanoparticle composites for enhanced cellular proliferation
    Gianni Ciofani
    Italian Institute of Technology, Smart Materials Lab, Center for Micro BioRobotics c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, Pontedera, Pisa, Italy
    Biomed Microdevices 13:255-66. 2011
  6. doi request reprint Assessing cytotoxicity of boron nitride nanotubes: Interference with the MTT assay
    Gianni Ciofani
    Italian Institute of Technology, Smart Materials Lab, Center of MicroBioRobotics at Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    Biochem Biophys Res Commun 394:405-11. 2010
  7. doi request reprint In vitro and in vivo biocompatibility testing of functionalized carbon nanotubes
    Gianni Ciofani
    Scuola Superiore Sant Anna, Pisa, Italy
    Methods Mol Biol 625:67-83. 2010
  8. pmc Barium Titanate Nanoparticles: Highly Cytocompatible Dispersions in Glycol-chitosan and Doxorubicin Complexes for Cancer Therapy
    Gianni Ciofani
    Center of MicroBioRobotics c o Scuola Superiore Sant Anna, Italian Institute of Technology, Viale Rinaldo Piaggio 34, 56025, Pontedera Pisa, Italy
    Nanoscale Res Lett 5:1093-101. 2010
  9. doi request reprint Evaluation of cytocompatibility and cell response to boron nitride nanotubes
    Gianni Ciofani
    Smart Materials Lab, Center of MicroBioRobotics, Italian Institute of Technology, Scuola Superiore Sant Anna, Pontedera, Pisa, Italy
    Methods Mol Biol 811:193-206. 2012
  10. ncbi request reprint Potential applications of boron nitride nanotubes as drug delivery systems
    Gianni Ciofani
    Center of MicroBioRobotics c o Scuola Superiore Sant Anna, Italian Institute of Technology, Smart Materials Lab, Viale Rinaldo Piaggio, 34 56025 Pontedera Pisa, Italy
    Expert Opin Drug Deliv 7:889-93. 2010

Detail Information

Publications47

  1. pmc Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
    G Ciofani
    Scuola Superiore Sant Anna, Pisa, Italy
    Int J Nanomedicine 5:285-98. 2010
    ..We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation...
  2. doi request reprint A simple approach to covalent functionalization of boron nitride nanotubes
    Gianni Ciofani
    Italian Institute of Technology, Smart Materials Lab, Center for MicroBioRobotics Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    J Colloid Interface Sci 374:308-14. 2012
    ..Since, in particular, biomedical applications are envisaged, we also demonstrated in vitro biocompatibility and cellular up-take of the functionalized BNNTs...
  3. doi request reprint Hypergravity effects on myoblast proliferation and differentiation
    Gianni Ciofani
    Italian Institute of Technology, Center of MicroBioRobotics c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    J Biosci Bioeng 113:258-61. 2012
    ....
  4. pmc Pilot in vivo toxicological investigation of boron nitride nanotubes
    Gianni Ciofani
    Italian Institute of Technology, Center of Micro BioRobotics c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, Pontedera Pisa, Italy
    Int J Nanomedicine 7:19-24. 2012
    ..Even if preliminary, the data are highly promising, as they showed no adverse effects on all the evaluated parameters, and therefore suggest the possibility of the realistic application of BNNTs in the biomedical field...
  5. doi request reprint Preparation, characterization and in vitro testing of poly(lactic-co-glycolic) acid/barium titanate nanoparticle composites for enhanced cellular proliferation
    Gianni Ciofani
    Italian Institute of Technology, Smart Materials Lab, Center for Micro BioRobotics c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, Pontedera, Pisa, Italy
    Biomed Microdevices 13:255-66. 2011
    ..Finally, the possibility to obtain porous scaffold and, therefore, an actual 3D culture system, is introduced...
  6. doi request reprint Assessing cytotoxicity of boron nitride nanotubes: Interference with the MTT assay
    Gianni Ciofani
    Italian Institute of Technology, Smart Materials Lab, Center of MicroBioRobotics at Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    Biochem Biophys Res Commun 394:405-11. 2010
    ..These results confirm the high complexity of these new nanomaterials, and the needing of extensive investigations on their exciting potential applications in the biomedical field...
  7. doi request reprint In vitro and in vivo biocompatibility testing of functionalized carbon nanotubes
    Gianni Ciofani
    Scuola Superiore Sant Anna, Pisa, Italy
    Methods Mol Biol 625:67-83. 2010
    ..Moreover, in vivo neurocompatibility tests were carried out through injections in mouse brains...
  8. pmc Barium Titanate Nanoparticles: Highly Cytocompatible Dispersions in Glycol-chitosan and Doxorubicin Complexes for Cancer Therapy
    Gianni Ciofani
    Center of MicroBioRobotics c o Scuola Superiore Sant Anna, Italian Institute of Technology, Viale Rinaldo Piaggio 34, 56025, Pontedera Pisa, Italy
    Nanoscale Res Lett 5:1093-101. 2010
    ..Our results suggest that innovative ceramic nanomaterials such as BTNPs can be realistically exploited as alternative cellular nanovectors...
  9. doi request reprint Evaluation of cytocompatibility and cell response to boron nitride nanotubes
    Gianni Ciofani
    Smart Materials Lab, Center of MicroBioRobotics, Italian Institute of Technology, Scuola Superiore Sant Anna, Pontedera, Pisa, Italy
    Methods Mol Biol 811:193-206. 2012
    ....
  10. ncbi request reprint Potential applications of boron nitride nanotubes as drug delivery systems
    Gianni Ciofani
    Center of MicroBioRobotics c o Scuola Superiore Sant Anna, Italian Institute of Technology, Smart Materials Lab, Viale Rinaldo Piaggio, 34 56025 Pontedera Pisa, Italy
    Expert Opin Drug Deliv 7:889-93. 2010
    ..Finally, the possibility of combining their physical and chemical properties is approached, highlighting the features that render these innovative nanovectors unique and exceptional candidates for many bio-applications...
  11. doi request reprint Enhancement of neurite outgrowth in neuronal-like cells following boron nitride nanotube-mediated stimulation
    Gianni Ciofani
    Italian Institute of Technology c o Scuola Superiore Sant Anna, Viale Rinaldo Piaggio 34 Pontedera Pisa, 56025, Italy
    ACS Nano 4:6267-77. 2010
    ..PC12 stimulated with the present method exhibit neurite sprout 30% greater than the control cultures after 9 days of treatment...
  12. doi request reprint Preparation of stable dispersion of barium titanate nanoparticles: Potential applications in biomedicine
    G Ciofani
    Scuola Superiore Sant Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy
    Colloids Surf B Biointerfaces 76:535-43. 2010
    ..Finally, we presented a systematic study of short-term cytotoxicity of the prepared dispersion based both on quantitative (metabolism, proliferation) and qualitative (apoptosis, viability, differentiation) assays...
  13. doi request reprint Urease loaded alginate microspheres for blood purification
    Gianni Ciofani
    CRIM Lab, Center for Research In Microengineering, Scuola Superiore Sant Anna, Viale Rinaldo Piaggio, 34 56025 Pontedera PI, Italy
    J Microencapsul 25:569-76. 2008
    ....
  14. ncbi request reprint Bicompartmental device for dynamic cell coculture: design, realisation and preliminary results
    Gianni Ciofani
    CRIM Lab, Center for Research In Microengineering, Scuola Superiore Sant Anna, Viale Rinaldo Piaggio, Pontedera, Italy
    J Biosci Bioeng 105:536-44. 2008
    ..In vitro tests performed on cell lines confirm the effectiveness of the device as an experimental tool for measurements in dynamic and multiple-cell cultures and cocultures...
  15. ncbi request reprint Characterization of an alginate-based drug delivery system for neurological applications
    Gianni Ciofani
    CRIM Center for Research in Microengineering Lab, Scuola Superiore Sant Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy
    Med Eng Phys 30:848-55. 2008
    ..Consequently, fibers release is tested in vitro and theoretically modelled, in order to obtain mathematical correlations between the release kinetics and key parameters affecting the realization procedure...
  16. doi request reprint Magnetic alginate microspheres: system for the position controlled delivery of nerve growth factor
    Gianni Ciofani
    Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33 56127 Pisa, Italy
    Biomed Microdevices 11:517-27. 2009
    ..These observations on the carriage and release of growth factors by the proposed microparticles open new therapeutic options for both neuronal regeneration and of the development of effective neuronal interfaces...
  17. ncbi request reprint Alginate and chitosan particles as drug delivery system for cell therapy
    Gianni Ciofani
    CRIM Lab Center for Research In Microengineering, Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33, 56127, Pisa, Italy
    Biomed Microdevices 10:131-40. 2008
    ..5 h(-1)). In addition, the particles degrade within 24 h from the up-take without side effects for cell viability...
  18. doi request reprint A bi-modal approach against cancer: magnetic alginate nanoparticles for combined chemotherapy and hyperthermia
    Gianni Ciofani
    Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33 56127 Pisa, Italy
    Med Hypotheses 73:80-2. 2009
    ..Here, we present a potential application that combines, in a single nanovector, efficient targeting, overcoming of bio-barriers, drug delivery, and physical disruption of tumor tissues...
  19. ncbi request reprint A drug delivery system based on alginate microspheres: mass-transport test and in vitro validation
    Gianni Ciofani
    CRIM Laboratory, Center of Research In Microengineering, Scuola Superiore Sant Anna, Piazza Martiri della, Libertà, 33 56127 Pisa, Italy
    Biomed Microdevices 9:395-403. 2007
    ..Preliminary results show that neuronal axons grow approaching the Netrin-1 source, thus indicating an efficient entrapment and release of the protein in the microspheres, in agreement with the microsphere modelling described before...
  20. ncbi request reprint Chemically functionalized carbon nanotubes: emerging vectors for cell therapy
    Gianni Ciofani
    Scuola Superiore Sant Anna, Pisa, Italy
    Mini Rev Med Chem 9:1251-61. 2009
    ....
  21. doi request reprint Cytocompatibility, interactions, and uptake of polyethyleneimine-coated boron nitride nanotubes by living cells: confirmation of their potential for biomedical applications
    Gianni Ciofani
    Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33 56127 Pisa, Italy
    Biotechnol Bioeng 101:850-8. 2008
    ..The results demonstrate good cytocompatibility together with unequivocal BNNT cellular uptake by an energy-dependent endocytic process...
  22. doi request reprint Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds
    Leonardo Ricotti
    BioRobotics Institute, Polo Sant Anna Valdera, Scuola Superiore Sant Anna, Pontedera Pisa, Italy
    Biomed Mater 7:035010. 2012
    ..We also clarified how the nanofibrous substrate influences myotube formation, and quantified a series of myotube-related parameters for both C2C12 and H9c2 cells...
  23. pmc Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels
    Leonardo Ricotti
    The BioRobotics Institute, Scuola Superiore Sant Anna, Pontedera, Pisa, Italy
    PLoS ONE 8:e71707. 2013
    ..Finally, calcium imaging-based experiments demonstrated a higher functionality of the stimulated co-cultures. ..
  24. doi request reprint Effects of cerium oxide nanoparticles on PC12 neuronal-like cells: proliferation, differentiation, and dopamine secretion
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Micro BioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy
    Pharm Res 30:2133-45. 2013
    ..Besides traditional anti-oxidant agents, in recent years much attention has been focused on nanotechnological solutions, including cerium oxide nanoparticles (nanoceria)...
  25. doi request reprint Bio/non-bio interfaces: a straightforward method for obtaining long term PDMS/muscle cell biohybrid constructs
    Giada Graziana Genchi
    Scuola Superiore Sant Anna, The BioRobotics Institute, Pisa, Italy
    Colloids Surf B Biointerfaces 105:144-51. 2013
    ..Furthermore, modified PDMS membranes prove to be suitable to several long-term studies of cell behaviour in vitro, including muscle cell contractility investigations...
  26. doi request reprint Flexible polymeric ultrathin film for mesenchymal stem cell differentiation
    Virginia Pensabene
    Istituto Italiano di Tecnologia, Center for MicroBioRobotics SSSA, Pontedera, Italy
    Acta Biomater 7:2883-91. 2011
    ..The nanofilms did not impair the growth and differentiation of osteoblasts and chondrocytes. Moreover, nanofilm adhesion to rabbit joints was evident under ex vivo conditions...
  27. ncbi request reprint Preparation of boron nitride nanotubes aqueous dispersions for biological applications
    Gianni Ciofani
    CRIM Lab Center for Research In Microengineering, Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33 56127 Pisa, Italy
    J Nanosci Nanotechnol 8:6223-31. 2008
    ..Experimental results showed a satisfactory cell viability up to a concentration of 5.0 microg/ml PEI-BNNTs in the cell culture medium...
  28. doi request reprint Transcriptional profile of genes involved in oxidative stress and antioxidant defense in PC12 cells following treatment with cerium oxide nanoparticles
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Micro BioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy Electronic address
    Biochim Biophys Acta 1840:495-506. 2014
    ....
  29. doi request reprint Effects of barium titanate nanoparticles on proliferation and differentiation of rat mesenchymal stem cells
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Micro BioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    Colloids Surf B Biointerfaces 102:312-20. 2013
    ..Finally, differentiation in adipocytes and osteocytes was achieved in the presence of high doses of BTNPs, thus highlighting the safety of these nanostructures towards mesenchymal stem cells...
  30. ncbi request reprint Biocompatibility of boron nitride nanotubes: an up-date of in vivo toxicological investigation
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for MicroBioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy
    Int J Pharm 444:85-8. 2013
    ..All the collected data are very promising, suggesting the optimal biocompatibility of BNNTs, and thus enabling their exploitation in nanomedicine as nanotransducers and nanocarriers...
  31. ncbi request reprint Transferrin-conjugated boron nitride nanotubes: protein grafting, characterization, and interaction with human endothelial cells
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for MicroBioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera Pisa, Italy
    Int J Pharm 436:444-53. 2012
    ....
  32. doi request reprint Human recombinant elastin-like protein coatings for muscle cell proliferation and differentiation
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Micro BioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy
    Acta Biomater 9:5111-21. 2013
    ..Cell behavior was analyzed in terms of adhesion, proliferation and differentiation. The collected data strongly suggest the use of HELPs as excellent biomaterials for tissue engineering and regenerative medicine applications...
  33. doi request reprint Nanostructured, highly aligned poly(hydroxy butyrate) electrospun fibers for differentiation of skeletal and cardiac muscle cells
    Leonardo Ricotti
    BioRobotics Institute, Scuola Superiore Sant Anna, and with the Center for Micro Bio Robotics SSSA, Istituto Italiano di Tecnologia, 56127 Pontedera, PI, Italy
    Conf Proc IEEE Eng Med Biol Soc 2011:3597-600. 2011
    ..Moreover, quantitative parameters for each cell line are provided to clarify which aspects of the differentiation process are influenced by the different matrix topographies...
  34. doi request reprint Physicochemical properties affecting cellular uptake of carbon nanotubes
    Vittoria Raffa
    Scuola Superiore Sant Anna, Medical Science Laboratory, 56127 Pisa, Italy
    Nanomedicine (Lond) 5:89-97. 2010
    ..This information may be relevant to the rational design of CNT-based carriers for cell therapy...
  35. ncbi request reprint Cytocompatibility evaluation of gum Arabic-coated ultra-pure boron nitride nanotubes on human cells
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy
    Nanomedicine (Lond) 9:773-88. 2014
    ..Original submitted 3 December 2013; Revised submitted 28 January 2014; Published online 6 February 2014. ..
  36. ncbi request reprint Cover picture: macromol. Biosci. 5/2014
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Micro BioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy
    Macromol Biosci 14:599. 2014
    ..The obtained microparticles are characterized in terms of morphology, structure, magnetic properties, drug release, and magnetic drivability, showing promise for further biomedical applications. ..
  37. pmc Barium titanate core--gold shell nanoparticles for hyperthermia treatments
    Elmira Farrokhtakin
    Center for Micro BioRobotics SSSA, Fondazione Istituto Italiano di Tecnologia, Pontedera, Pisa, Italy
    Int J Nanomedicine 8:2319-31. 2013
    ....
  38. doi request reprint Gd-doped BNNTs as T2-weighted MRI contrast agents
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Viale Rinaldo Piaggio 34, Pontedera, Pisa, Italy
    Nanotechnology 24:315101. 2013
    ..Finally, we demonstrate their complete biocompatibility in vitro on human neuroblastoma cells, together with their ability to effectively label and affect contrast in MRI images at 7 T. ..
  39. doi request reprint Carbon nanotube-enhanced cell electropermeabilisation
    Vittoria Raffa
    Medical Science Lab, Scuola Superiore Sant Anna, Piazza Martiri della Libertà 33, Pisa, Italy
    Bioelectrochemistry 79:136-41. 2010
    ....
  40. doi request reprint Pluronic-coated carbon nanotubes do not induce degeneration of cortical neurons in vivo and in vitro
    Giuseppe Bardi
    Institute of Neurosciences, National Research Council, Pisa, Italy
    Nanomedicine 5:96-104. 2009
    ..However, the presence of MWCNTs can avoid PF127-induced apoptosis. These results suggest that PF127-coated MWCNTs do not induce apoptosis of cortical neurons. Moreover, the presence of MWCNTs can reduce PF127 toxicity...
  41. ncbi request reprint Recombinant human elastin-like magnetic microparticles for drug delivery and targeting
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Micro BioRobotics SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy
    Macromol Biosci 14:632-42. 2014
    ..The obtained microparticles are characterized in terms of morphology, structure, magnetic properties, drug release, and magnetic drivability, showing interesting and promising results for further biomedical applications. ..
  42. doi request reprint Boron nitride nanotubes: biocompatibility and potential spill-over in nanomedicine
    Gianni Ciofani
    Istituto Italiano di Tecnologia, Center for Viale Rinaldo Piaggio 34 Pontedera Pisa 56025, Italy
    Small 9:1672-85. 2013
    ..Finally, the appealing diagnostic and therapeutic opportunities offered by BNNT-based systems are described, envisioning the potential spill-over effects of such 'smart' and 'active' nanoparticles in nanomedicine...
  43. pmc Combination of polymer technology and carbon nanotube array for the development of an effective drug delivery system at cellular level
    Cristina Riggio
    Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33, 56127, Pisa, Italy
    Nanoscale Res Lett 4:668-73. 2009
    ..The implication of this study opens several perspectives in particular in the field of neurointerfaces, combining several functions into a single platform...
  44. ncbi request reprint Gold nanoshell/polysaccharide nanofilm for controlled laser-assisted tissue thermal ablation
    Eugenio Redolfi Riva
    Center for Micro BioRobotics, Istituto Italiano di Tecnologia, Pontedera, 56025, Italy
    ACS Nano 8:5552-63. 2014
    ..Thermonanofilms can find applications for local photothermal treatment of cancerous lesions and wherever high precision and control of heat treatment is required. ..
  45. ncbi request reprint Two-photon polymerization of sub-micrometric patterned surfaces: investigation of cell-substrate interactions and improved differentiation of neuron-like cells
    Attilio Marino
    Center for Micro BioRobotics SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy
    ACS Appl Mater Interfaces 5:13012-21. 2013
    ..Collectively, we showed as submicrometric structures fabricated by DLW can be used as a useful tool for the study of the axon mechanobiology. ..
  46. doi request reprint A hybrid approach for the control of axonal outgrowth: preliminary simulation results
    Gianni Ciofani
    Arts, CRIM and IIT Labs, Scuola Superiore Sant Anna, Piazza Martiri della Libertà, 33 56127 Pisa, Italy
    Med Biol Eng Comput 49:163-70. 2011
    ..Moreover, the model can be easily extended to a three-dimensional environment...