Flavia Valtorta

Summary

Affiliation: San Raffaele Scientific Institute
Country: Italy

Publications

  1. ncbi request reprint Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals
    A Menegon
    Dept Neuroscience, San Raffaele Scientific Institute, Milan, Italy
    J Cell Sci 113:3573-82. 2000
  2. pmc Nonsense-mediated mRNA decay and loss-of-function of the protein underlie the X-linked epilepsy associated with the W356× mutation in synapsin I
    Maila Giannandrea
    Division of Neuroscience, San Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    PLoS ONE 8:e67724. 2013
  3. ncbi request reprint Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis?
    Flavia Valtorta
    Department of Neuroscience, S Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    Bioessays 26:445-53. 2004
  4. ncbi request reprint Protein kinase A-mediated synapsin I phosphorylation is a central modulator of Ca2+-dependent synaptic activity
    Andrea Menegon
    San Raffaele Scientific Institute and Vita Salute University, 20132 Milan, Italy
    J Neurosci 26:11670-81. 2006
  5. ncbi request reprint Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons
    Dario Bonanomi
    Department of Neuroscience, San Raffaele Scientific Institute, Vita Salute University, 20132 Milan, Italy
    J Neurosci 25:7299-308. 2005
  6. pmc Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles
    Maria Pennuto
    Department of Neuroscience, San Raffaele Scientific Institute and Vita Salute University, 20132 Milano, Italy
    Mol Biol Cell 14:4909-19. 2003
  7. pmc Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2
    Dario Bonanomi
    San Raffaele Scientific Institute and Vita Salute University, Via Olgettina 58, 20132 Milan, Italy
    Biochem J 404:525-34. 2007
  8. doi request reprint Effects of phosphorylation and neuronal activity on the control of synapse formation by synapsin I
    Laura E Perlini
    San Raffaele Scientific Institute and Vita Salute University, Via Olgettina 58, 20132 Milano, Italy
    J Cell Sci 124:3643-53. 2011
  9. pmc {alpha}-synuclein and its A30P mutant affect actin cytoskeletal structure and dynamics
    Vítor L Sousa
    Department of Neuroscience, San Raffaele Scientific Institute, Milan, Italy
    Mol Biol Cell 20:3725-39. 2009
  10. doi request reprint The role of synapsins in neuronal development
    Eugenio F Fornasiero
    San Raffaele Scientific Institute, Vita Salute University, Via Olgettina 58, 20132, Milan, Italy
    Cell Mol Life Sci 67:1383-96. 2010

Collaborators

  • Fabio Benfenati
  • Andrea Contestabile
  • Hung Teh Kao
  • Pietro Baldelli
  • Niels H Gehring
  • Giancarlo Comi
  • Patrick Collombat
  • Jean Antoine Girault
  • Ivan de Curtis
  • P Greengard
  • Mirella Ghirardi
  • Michela Matteoli
  • Claudia Verderio
  • C Montecucco
  • Dario Bonanomi
  • Eugenio F Fornasiero
  • Andrea Menegon
  • Maila Giannandrea
  • Maria Pennuto
  • Silvia Giovedi
  • Anna Fassio
  • Anna Corradi
  • Davide Pozzi
  • Marco Cambiaghi
  • Elena Monzani
  • Fabrizia C Guarnieri
  • Pierluigi Valente
  • Michela Rigoni
  • Laura E Perlini
  • Jacopo Meldolesi
  • Vítor L Sousa
  • Gaia Colasante
  • Franco Onofri
  • Sara Gualdoni
  • Chiara Albertinazzi
  • Giambattista Bonanno
  • Giuliana Ferrari
  • Daniela Merlo
  • Ornella Rossetto
  • François Darchen
  • Ferdinando Fiumara
  • Enrico Ferrea
  • Davide Boido
  • Letizia Leocani
  • Gabriele Lignani
  • Pellegrino Lippiello
  • Francesco Paonessa
  • Fabio Minicucci
  • Andreas E Kulozik
  • Marco Cursi
  • Rosalba D'Alessandro
  • Riccardo Fesce
  • Andrea Raimondi
  • Anne M J Verstegen
  • Silvia Casagrande
  • Marta Orlando
  • Thierry Nieus
  • Luigi Naldini
  • Mario Amendola
  • Francesca Botti
  • A Menegon
  • Serena Bellani
  • Malikmohamed Yousuf
  • Evelina Chieregatti
  • Carmelo Ferrai
  • Vania Broccoli
  • Kazuaki Yoshikawa
  • Mario Maira
  • Valentina Raimondi
  • John L R Rubenstein
  • Ahmed Mansouri
  • Angela Bachi
  • Mirko Messa
  • Laura Rusconi
  • Sara Corbetta
  • Chiara Agnese Colombo
  • Vittoria Matafora
  • Maurizio Mete
  • Simona Zappettini
  • Egidio D'Angelo
  • Francesco Lotti
  • Jonathan Mapelli
  • Annarita Miccio
  • Giampietro Schiavo
  • Federica Allegrini
  • Anne E Weston
  • Paola Caccin
  • Paola Vaccaro
  • Pier Giorgio Montarolo
  • Chiara Milanese

Detail Information

Publications29

  1. ncbi request reprint Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals
    A Menegon
    Dept Neuroscience, San Raffaele Scientific Institute, Milan, Italy
    J Cell Sci 113:3573-82. 2000
    ....
  2. pmc Nonsense-mediated mRNA decay and loss-of-function of the protein underlie the X-linked epilepsy associated with the W356× mutation in synapsin I
    Maila Giannandrea
    Division of Neuroscience, San Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    PLoS ONE 8:e67724. 2013
    ....
  3. ncbi request reprint Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis?
    Flavia Valtorta
    Department of Neuroscience, S Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    Bioessays 26:445-53. 2004
    ..In this article, we summarise the main results of the studies on Syp carried out by our and other laboratories, and explain why we believe that Syp plays a major role in SV trafficking...
  4. ncbi request reprint Protein kinase A-mediated synapsin I phosphorylation is a central modulator of Ca2+-dependent synaptic activity
    Andrea Menegon
    San Raffaele Scientific Institute and Vita Salute University, 20132 Milan, Italy
    J Neurosci 26:11670-81. 2006
    ..These data define a hierarchical crosstalk between cAMP- and CaM-dependent cascades and point to synapsin as a major effector of PKA in the modulation of activity-dependent SV exocytosis...
  5. ncbi request reprint Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons
    Dario Bonanomi
    Department of Neuroscience, San Raffaele Scientific Institute, Vita Salute University, 20132 Milan, Italy
    J Neurosci 25:7299-308. 2005
    ....
  6. pmc Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles
    Maria Pennuto
    Department of Neuroscience, San Raffaele Scientific Institute and Vita Salute University, 20132 Milano, Italy
    Mol Biol Cell 14:4909-19. 2003
    ..These data support a role for SypI in directing the correct sorting of VAMP2 in neurons and demonstrate that a direct interaction between the two proteins is required for SypI in order to exert its effect...
  7. pmc Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2
    Dario Bonanomi
    San Raffaele Scientific Institute and Vita Salute University, Via Olgettina 58, 20132 Milan, Italy
    Biochem J 404:525-34. 2007
    ....
  8. doi request reprint Effects of phosphorylation and neuronal activity on the control of synapse formation by synapsin I
    Laura E Perlini
    San Raffaele Scientific Institute and Vita Salute University, Via Olgettina 58, 20132 Milano, Italy
    J Cell Sci 124:3643-53. 2011
    ..Altogether these results indicate that Syn I is an important regulator of synapse formation, which adjusts synapse number in response to extracellular signals...
  9. pmc {alpha}-synuclein and its A30P mutant affect actin cytoskeletal structure and dynamics
    Vítor L Sousa
    Department of Neuroscience, San Raffaele Scientific Institute, Milan, Italy
    Mol Biol Cell 20:3725-39. 2009
    ..Actin cytoskeletal disruption induced by the A30P mutant might alter various cellular processes and thereby play a role in the pathogenesis of neurodegeneration...
  10. doi request reprint The role of synapsins in neuronal development
    Eugenio F Fornasiero
    San Raffaele Scientific Institute, Vita Salute University, Via Olgettina 58, 20132, Milan, Italy
    Cell Mol Life Sci 67:1383-96. 2010
    ..In this review, we describe the main functional features of the synapsins, in relation to the complex role played by these phosphoproteins in neuronal development...
  11. pmc Fluorescence resonance energy transfer detection of synaptophysin I and vesicle-associated membrane protein 2 interactions during exocytosis from single live synapses
    Maria Pennuto
    Department of Neuroscience, S Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    Mol Biol Cell 13:2706-17. 2002
    ....
  12. doi request reprint Synapsins contribute to the dynamic spatial organization of synaptic vesicles in an activity-dependent manner
    Eugenio F Fornasiero
    San Raffaele Scientific Institute and Vita Salute University, I 20132 Milan, Italy
    J Neurosci 32:12214-27. 2012
    ....
  13. pmc Temporal evolution of neurophysiological and behavioral features of synapsin I/II/III triple knock-out mice
    Marco Cambiaghi
    San Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    Epilepsy Res 103:153-60. 2013
    ..This aspect might be connected to brain dysfunction often associated to epilepsy in the interictal period...
  14. ncbi request reprint Taipoxin induces synaptic vesicle exocytosis and disrupts the interaction of synaptophysin I with VAMP2
    Dario Bonanomi
    Department of Neuroscience, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    Mol Pharmacol 67:1901-8. 2005
    ..These early effects of the toxin are followed by severe impairment of SV exo-endocytosis, which might underlie the prevention of neurotransmitter release reported after intoxication by taipoxin...
  15. ncbi request reprint Phosphorylation by cAMP-dependent protein kinase is essential for synapsin-induced enhancement of neurotransmitter release in invertebrate neurons
    Ferdinando Fiumara
    Department of Neuroscience, Section of Physiology, University of Torino, Corso Raffaello 30, 10125, Italy
    J Cell Sci 117:5145-54. 2004
    ....
  16. doi request reprint The synapsins: multitask modulators of neuronal development
    Flavia Valtorta
    San Raffaele Scientific Institute and Vita Salute University, Via Olgettina 58, Milano, Italy
    Semin Cell Dev Biol 22:378-86. 2011
    ....
  17. ncbi request reprint Normal levels of Rac1 are important for dendritic but not axonal development in hippocampal neurons
    Sara Gualdoni
    DIBIT, San Raffaele Scientific Institute, Milano, Italy
    Biol Cell 99:455-64. 2007
    ..There are two distinct Rac proteins expressed in neurons, namely the ubiquitous Rac1 and the neuron-specific Rac3. The specific functions of each of these GTPases during early neuronal development are largely unknown...
  18. ncbi request reprint Protein sorting in the synaptic vesicle life cycle
    Dario Bonanomi
    Department of Neuroscience, San Raffaele Scientific Institute and Vita Salute University, Milan, Italy
    Prog Neurobiol 80:177-217. 2006
    ..As a result of both processes, SVs with heterogeneous molecular make-up, and hence displaying variable competence for exocytosis, may be generated and coexist within the same nerve terminal...
  19. ncbi request reprint Localization of focal adhesion kinase isoforms in cells of the central nervous system
    Andrea Contestabile
    Department of Neuroscience, S Raffaele Scientific Institute and Vita Salute University, Milano, Italy
    Int J Dev Neurosci 21:83-93. 2003
    ....
  20. ncbi request reprint Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons
    Andrea Menegon
    Department of Neuroscience, S Raffaele Scientific Institute and Vita Salute University, 20132 Milano, Italy
    J Neurosci 22:7016-26. 2002
    ..In the presence of a nearly homogeneous subcellular distribution, this highly regionalized regulation of activity may reflect the multifunctional roles of CaMKII in both developing and mature neurons...
  21. pmc REST/NRSF-mediated intrinsic homeostasis protects neuronal networks from hyperexcitability
    Davide Pozzi
    1 Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy 2 Department of Neuroscience, San Raffaele Scientific Institute, Vita Salute University, Milano, Italy
    EMBO J 32:2994-3007. 2013
    ..Our results identify REST/NRSF as a critical factor linking neuronal activity to the activation of intrinsic homeostasis and restoring a physiological level of activity in the entire neuronal network. ..
  22. pmc Arx is a direct target of Dlx2 and thereby contributes to the tangential migration of GABAergic interneurons
    Gaia Colasante
    Department of Biological and Technological Research, San Raffaele Scientific Institute, Stem Cell Research Institute, Vita e Salute San Raffaele University, 20132 Milan, Italy
    J Neurosci 28:10674-86. 2008
    ..These findings contribute elucidating the genetic hierarchy that likely underlies the etiology of a variety of human neurodevelopmental disorders...
  23. doi request reprint Site-specific synapsin I phosphorylation participates in the expression of post-tetanic potentiation and its enhancement by BDNF
    Pierluigi Valente
    Section of Physiology, Department of Experimental Medicine, University of Genoa and National Institute of Neuroscience, 16132 Genova, Italy
    J Neurosci 32:5868-79. 2012
    ....
  24. ncbi request reprint The synapsin domain E accelerates the exoendocytotic cycle of synaptic vesicles in cerebellar Purkinje cells
    Anna Fassio
    Center of Neuroscience and Neuroengineering, Department of Experimental Medicine, University of Genoa, Italy
    J Cell Sci 119:4257-68. 2006
    ..These effects may increase the sensitivity of postsynaptic neurons to inhibition and thereby contribute to the inhibitory control of network activity...
  25. ncbi request reprint Lack of synapsin I reduces the readily releasable pool of synaptic vesicles at central inhibitory synapses
    Pietro Baldelli
    Department of Neuroscience and Brain Technologies, The Italian Institute of Technology, 16163 Genova, Italy
    J Neurosci 27:13520-31. 2007
    ..The decreased size of the SV readily releasable pool, coupled with a decreased SV recycling rate and refilling by the SV reserve pool, may contribute to the epileptic phenotype of SynI knock-out mice...
  26. ncbi request reprint Synapsin is a novel Rab3 effector protein on small synaptic vesicles. II. Functional effects of the Rab3A-synapsin I interaction
    Silvia Giovedi
    Department of Experimental Medicine, Section of Human Physiology, University of Genova, Via Benedetto XV, 16132, Italy
    J Biol Chem 279:43769-79. 2004
    ..Given the well recognized importance of both synapsins and Rab3A in synaptic vesicles exocytosis, this interaction is likely to play a major role in the modulation of neurotransmitter release...
  27. ncbi request reprint Synapsin is a novel Rab3 effector protein on small synaptic vesicles. I. Identification and characterization of the synapsin I-Rab3 interactions in vitro and in intact nerve terminals
    Silvia Giovedi
    Department of Experimental Medicine, Section of Human Physiology, University of Genova, Via Benedetto XV, 16132, Italy
    J Biol Chem 279:43760-8. 2004
    ..The data indicate that synapsin I is a novel Rab3 interactor on synaptic vesicles and suggest that the synapsin-Rab3 interaction may participate in the regulation of synaptic vesicle trafficking within the nerve terminals...
  28. ncbi request reprint Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles
    Michela Rigoni
    Department of Biomedical Sciences and CNR Institute of Neuroscience, University of Padova, Viale G Colombo 3, 35121 Padova, Italy
    J Cell Sci 117:3561-70. 2004
    ..These changes closely resemble those occurring at neuromuscular junctions of intoxicated animals and fully qualify these primary neuronal cultures as pertinent models for studying the molecular mode of action of these neurotoxins...
  29. ncbi request reprint Synapsin phosphorylation by SRC tyrosine kinase enhances SRC activity in synaptic vesicles
    Franco Onofri
    Department of Experimental Medicine, University of Genova, 16132 Genova, Italy
    J Biol Chem 282:15754-67. 2007
    ..We conclude that synapsin is an endogenous substrate and activator of synaptic vesicle-associated c-Src and that regulation of Src activity on synaptic vesicles participates in the regulation of neurotransmitter release by synapsin...