Paolo Lusso

Summary

Affiliation: San Raffaele Scientific Institute
Country: Italy

Publications

  1. pmc Cryptic nature of a conserved, CD4-inducible V3 loop neutralization epitope in the native envelope glycoprotein oligomer of CCR5-restricted, but not CXCR4-using, primary human immunodeficiency virus type 1 strains
    Paolo Lusso
    Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    J Virol 79:6957-68. 2005
  2. pmc HIV and the chemokine system: 10 years later
    Paolo Lusso
    Unit of Human Virology, San Raffaele Scientific Institute, Milan, Italy
    EMBO J 25:447-56. 2006
  3. doi request reprint Biological and physical characterization of the X4 HIV-1 suppressive factor secreted by LPS-stimulated human macrophages
    Joanna Mikulak
    Human Virology Unit, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milano, Italy
    Virology 390:37-44. 2009
  4. ncbi request reprint HIV and chemokines: implications for therapy and vaccine
    Paolo Lusso
    Unit of Human Virology, DIBIT San Raffaele Scientific Institute, Via Olgettina no 58, 20132 Milan, Italy
    Vaccine 20:1964-7. 2002
  5. ncbi request reprint HHV-6 and the immune system: mechanisms of immunomodulation and viral escape
    Paolo Lusso
    Unit of Human Virology, Department of Biological and Technical Research DIBIT, San Rafaele Scientific Institute, Milano, Italy
    J Clin Virol 37:S4-10. 2006
  6. pmc Human herpesvirus 6A accelerates AIDS progression in macaques
    Paolo Lusso
    Unit of Human Virology, San Raffaele Scientific Institute, 20132 Milan, Italy
    Proc Natl Acad Sci U S A 104:5067-72. 2007
  7. pmc Broad-spectrum inhibition of HIV-1 by a monoclonal antibody directed against a gp120-induced epitope of CD4
    Samuele E Burastero
    Department of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy
    PLoS ONE 6:e22081. 2011
  8. ncbi request reprint A relapsing inflammatory syndrome and active human herpesvirus 8 infection
    Lorenzo Dagna
    Università Vita Salute San Raffaele School of Medicine, Milan, Italy
    N Engl J Med 353:156-63. 2005
  9. ncbi request reprint Selective suppression of IL-12 production by human herpesvirus 6
    Alison Smith
    Unit of Human Virology, Department of Biological and Technological Research, San Raffaele Scientific Institute, Via Olgettina n 58, Milan 20132, Italy
    Blood 102:2877-84. 2003
  10. doi request reprint Enhancement of anti-HIV-1 activity by hot spot evolution of RANTES-derived peptides
    Massimiliano Secchi
    Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, Milan 20132, Italy
    Chem Biol 19:1579-88. 2012

Collaborators

Detail Information

Publications47

  1. pmc Cryptic nature of a conserved, CD4-inducible V3 loop neutralization epitope in the native envelope glycoprotein oligomer of CCR5-restricted, but not CXCR4-using, primary human immunodeficiency virus type 1 strains
    Paolo Lusso
    Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    J Virol 79:6957-68. 2005
    ....
  2. pmc HIV and the chemokine system: 10 years later
    Paolo Lusso
    Unit of Human Virology, San Raffaele Scientific Institute, Milan, Italy
    EMBO J 25:447-56. 2006
    ..The imminent clinical availability of coreceptor-targeted viral entry inhibitors raises new hope for bridging the gap towards a definitive cure of HIV infection...
  3. doi request reprint Biological and physical characterization of the X4 HIV-1 suppressive factor secreted by LPS-stimulated human macrophages
    Joanna Mikulak
    Human Virology Unit, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milano, Italy
    Virology 390:37-44. 2009
    ..The biological and physical characterization of MDAF may be instrumental in devising effective new strategies for its identification...
  4. ncbi request reprint HIV and chemokines: implications for therapy and vaccine
    Paolo Lusso
    Unit of Human Virology, DIBIT San Raffaele Scientific Institute, Via Olgettina no 58, 20132 Milan, Italy
    Vaccine 20:1964-7. 2002
    ..Altogether, these findings provide a theoretical foundation for the rational design of safe and effective HIV-coreceptor inhibitors...
  5. ncbi request reprint HHV-6 and the immune system: mechanisms of immunomodulation and viral escape
    Paolo Lusso
    Unit of Human Virology, Department of Biological and Technical Research DIBIT, San Rafaele Scientific Institute, Milano, Italy
    J Clin Virol 37:S4-10. 2006
    ..By modulating specific antiviral immune responses, HHV-6 can facilitate its own spread and persistence in vivo, as well as enhance the pathogenic effects of other agents, such as human immunodeficiency virus...
  6. pmc Human herpesvirus 6A accelerates AIDS progression in macaques
    Paolo Lusso
    Unit of Human Virology, San Raffaele Scientific Institute, 20132 Milan, Italy
    Proc Natl Acad Sci U S A 104:5067-72. 2007
    ..Rapid disease development in dually infected animals was heralded by an early depletion of both CD4(+) and CD8(+) T cells. These results provide in vivo evidence that HHV-6A may act as a promoting factor in AIDS progression...
  7. pmc Broad-spectrum inhibition of HIV-1 by a monoclonal antibody directed against a gp120-induced epitope of CD4
    Samuele E Burastero
    Department of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy
    PLoS ONE 6:e22081. 2011
    ..Due to its broad-spectrum anti-HIV-1 activity and lack of immunosuppressive effects, a humanized derivative of MAb DB81 could provide a useful complement to current preventive or therapeutic strategies against HIV-1...
  8. ncbi request reprint A relapsing inflammatory syndrome and active human herpesvirus 8 infection
    Lorenzo Dagna
    Università Vita Salute San Raffaele School of Medicine, Milan, Italy
    N Engl J Med 353:156-63. 2005
    ..Histologic examination of an enlarged lymph node heavily infected with HHV-8 revealed an atypical lymphoproliferative disorder characterized by paracortical hyperplasia and collapsed primary and secondary follicles...
  9. ncbi request reprint Selective suppression of IL-12 production by human herpesvirus 6
    Alison Smith
    Unit of Human Virology, Department of Biological and Technological Research, San Raffaele Scientific Institute, Via Olgettina n 58, Milan 20132, Italy
    Blood 102:2877-84. 2003
    ..These data suggest that HHV-6 can dramatically affect the generation of effective cellular immune responses, providing a novel potential mechanism of HHV-6-mediated immunosuppression...
  10. doi request reprint Enhancement of anti-HIV-1 activity by hot spot evolution of RANTES-derived peptides
    Massimiliano Secchi
    Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, Milan 20132, Italy
    Chem Biol 19:1579-88. 2012
    ..These modified peptides represent leads for the development of effective HIV-1 inhibitors and microbicides...
  11. ncbi request reprint Alpha-defensins block the early steps of HIV-1 infection: interference with the binding of gp120 to CD4
    Lucinda Furci
    Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Milan, Italy
    Blood 109:2928-35. 2007
    ..These data demonstrate that alpha-defensins specifically block the initial phase of the HIV infectious cycle and modulate the expression of CD4, a critical receptor in the physiology of T-cell activation...
  12. pmc Viral replication-independent blockade of dendritic cell maturation and interleukin-12 production by human herpesvirus 6
    Alison P Smith
    Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Via Olgettina no 58, 20132 Milan, Italy
    J Virol 79:2807-13. 2005
    ..Altogether, these data identify interference with the functional maturation of DC as a potential mechanism of HHV-6-mediated immunosuppression...
  13. ncbi request reprint Chemokines as natural HIV antagonists
    Alessia Verani
    Unit of Human Virology, DIBIT San Raffaele Scientific Institute, Via Olgettina n 58, 20132 Milano, Italy
    Curr Mol Med 2:691-702. 2002
    ....
  14. pmc Engineering of Lactobacillus jensenii to secrete RANTES and a CCR5 antagonist analogue as live HIV-1 blockers
    Luca Vangelista
    Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, 20132 Milan, Italy
    Antimicrob Agents Chemother 54:2994-3001. 2010
    ..jensenii-produced C1C5 RANTES to block HIV-1 infection of CD4(+) T cells and macrophages, setting the basis for the development of a live anti-HIV-1 microbicide targeting CCR5 in an antagonistic manner...
  15. pmc Protective versus pathogenic anti-CD4 immunity: insights from the study of natural resistance to HIV infection
    Samuele E Burastero
    Unit of Clinical and Molecular Allergy, Division of Immunology, Infectious Diseases and Transplants, San Raffaele Scientific Institute, Milan, 20132, Italy
    J Transl Med 7:101. 2009
    ..The experimentum naturae of naturally resistant individuals indicates a strategy to design innovative strategies to neutralize HIV-1 by acting on the sharp edge between harmful and protective self-reactivity...
  16. doi request reprint A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load
    Mauro S Malnati
    Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Via Olgettina n 58, 20132 Milan, Italy
    Nat Protoc 3:1240-8. 2008
    ..An accurate quantification of the HIV-1 proviral load can be achieved within 1 d from blood withdrawal...
  17. ncbi request reprint Inhibition of CXCR4-tropic HIV-1 infection by lipopolysaccharide: evidence of different mechanisms in macrophages and T lymphocytes
    Alessia Verani
    Human Virology Unit, DIBIT, San Raffaele Scientific Institute, Milan, Italy
    J Immunol 168:6388-95. 2002
    ..These results suggest the existence of potent HIV-1 inhibitory factor(s), uncharacterized to date, released by activated cells of the mononuclear phagocytic system...
  18. pmc Inhibition of HIV-1 infection by human α-defensin-5, a natural antimicrobial peptide expressed in the genital and intestinal mucosae
    Lucinda Furci
    Unit of Human Virology, Department of Biological and Technological Research, San Raffaele Scientific Institute, Milan, Italy
    PLoS ONE 7:e45208. 2012
    ..Despite low amino acid identity, human defensin-5 (HD5) shares with HNPs a high degree of structural homology...
  19. pmc Critical role of the N-loop and beta1-strand hydrophobic clusters of RANTES-derived peptides in anti-HIV activity
    Luca Vangelista
    Unit of Human Virology, San Raffaele Scientific Institute, Via Olgettina n 58, 20132 Milan, Italy
    Biochem Biophys Res Commun 351:664-8. 2006
    ..These results may provide instrumental knowledge for the rational design of RANTES-derivative molecules with increased anti-HIV activity...
  20. pmc Calibrated real-time PCR assay for quantitation of human herpesvirus 8 DNA in biological fluids
    Francesco Broccolo
    Unit of Human Virology, DIBIT, San Raffele Scientific Institute, 20132 Milan, Italy
    J Clin Microbiol 40:4652-8. 2002
    ..In conclusion, our calibrated real-time PCR assay provides a reliable high-throughput method for quantitation of HHV-8 DNA in clinical and laboratory specimens...
  21. ncbi request reprint Additional evidence that pityriasis rosea is associated with reactivation of human herpesvirus-6 and -7
    Francesco Broccolo
    Unit of Human Virology, DIBIT San Raffaele Scientific Institute, Milan, Italy
    J Invest Dermatol 124:1234-40. 2005
    ..These data confirm the causal association between PR and active HHV-7 or, to a lesser extent, HHV-6 infection...
  22. pmc Molecular engineering of RANTES peptide mimetics with potent anti-HIV-1 activity
    Paolo Lusso
    Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Milan, Italy
    FASEB J 25:1230-43. 2011
    ..RANTES peptide mimetics provide new leads for the development of safe and effective HIV-1 entry inhibitors...
  23. ncbi request reprint Expression of CD4 on human peripheral blood neutrophils
    Priscilla Biswas
    Laboratory of Clinical Immunology, San Raffaele Scientific Institute, Milan, Italy
    Blood 101:4452-6. 2003
    ..Owing to their abundance in peripheral blood, CD4+ neutrophils may influence significantly the biodistribution of HIV delivering it to sites of inflammation or to additional tissue reservoirs...
  24. ncbi request reprint Differentiated human neural stem cells: a new ex vivo model to study HHV-6 infection of the central nervous system
    Lidia De Filippis
    Stem Cell Research Institute, DIBIT, San Raffaele Scientific Institute 20132 Milan, Milan, Italy
    J Clin Virol 37:S27-32. 2006
    ....
  25. pmc Rational design of novel HIV-1 entry inhibitors by RANTES engineering
    Luca Vangelista
    Department of Biological and Technological Research, San Raffaele Scientific Institute, Via Olgettina n 58, 20132 Milan, Italy
    Vaccine 26:3008-15. 2008
    ..Here, we present an overview of this rapidly evolving field, highlighting the most important features of RANTES molecular architecture and structure-function relationships...
  26. ncbi request reprint Human herpesvirus 8 (HHV-8/KSHV) and hematologic malignancies
    Mauro S Malnati
    Unit of Human Virology DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milano, Italy
    Rev Clin Exp Hematol 7:375-405. 2003
    ..As seen with KS, the incidence of HHV-8-associated lymphoproliferative disorders is increased in the setting of human immunodeficiency virus infection...
  27. pmc Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays
    Francesco Broccolo
    Unit of Human Virology, San Raffaele Scientific Institute, Milan, Italy
    J Clin Microbiol 41:4565-72. 2003
    ..Therefore, these assays represent a fully controlled high-throughput system for the evaluation of mycobacterial burden in clinical specimens...
  28. doi request reprint Selective reactivation of human herpesvirus 6 in patients with autoimmune connective tissue diseases
    Francesco Broccolo
    Department of Health Sciences, University of Milano Bicocca, Milano, Italy
    J Med Virol 85:1925-34. 2013
    ..J Med. Virol. 85:1925-1934, 2013. © 2013 Wiley Periodicals, Inc. ..
  29. doi request reprint Calibrated real-time polymerase chain reaction for specific quantitation of HHV-6A and HHV-6B in clinical samples
    Giulia Cassina
    Unit of Human Virology Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    J Virol Methods 189:172-9. 2013
    ..The new qPCR methods provide sensitive and specific tool for monitoring HHV-6A/B DNA load in clinical samples, facilitating the study of these viruses in human diseases...
  30. doi request reprint Molecular study of human herpesvirus 6 and 8 involvement in coronary atherosclerosis and coronary instability
    Marco Magnoni
    Department of Cardiothoracic and Vascular Diseases, University Vita Salute, San Raffaele Scientific Institute, Milan, Italy
    J Med Virol 84:1961-6. 2012
    ..However, an unusually high rate of active HHV-6 infection was documented among patients without atherosclerosis admitted to hospital with cardiac disease...
  31. doi request reprint A multiplex calibrated real-time PCR assay for quantitation of DNA of EBV-1 and 2
    Francesca Gatto
    Unit of Human Virology and Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    J Virol Methods 178:98-105. 2011
    ..In conclusion, this new assay provides a reliable high-throughput method for the quantitation of EBV DNA in clinical samples...
  32. doi request reprint A new antigen scanning strategy for monitoring HIV-1 specific T-cell immune responses
    Mauro S Malnati
    San Raffaele Scientific Institute, Milan, Italy
    J Immunol Methods 375:46-56. 2012
    ..Moreover, this peptide design allowed a marked reduction of the peptides number, representing a powerful tool for investigating novel HIV-1 candidate vaccine antigens in cohorts of HIV-seronegative and seropositive individuals...
  33. ncbi request reprint Non-cytotoxic inhibition of HIV-1 infection by unstimulated CD8+ T lymphocytes from HIV-exposed-uninfected individuals
    Lucinda Furci
    Unit of Human Virology, DIBIT, San Raffaele Scientific Institute, Via Olgettina n 58, Milan 20132, Italy
    AIDS 16:1003-8. 2002
    ..We investigated the ability of unstimulated CD8+ T lymphocytes from EU individuals to inhibit HIV-1 infection...
  34. pmc The superior folding of a RANTES analogue expressed in lactobacilli as compared to mammalian cells reveals a promising system to screen new RANTES mutants
    Massimiliano Secchi
    Unit of Human Virology, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    Protein Expr Purif 68:34-41. 2009
    ....
  35. ncbi request reprint Retrospective analysis of HHV-8 viremia and cellular viral load in HIV-seropositive patients receiving interleukin 2 in combination with antiretroviral therapy
    Mauro Malnati
    Unit of Human Virology, AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Milan, Italy
    Blood 100:1575-8. 2002
    ..Thus, IL-2 therapy seems safe in most patients infected with both HIV and HHV-8, except for those with detectable HHV-8 viremia, who may not be eligible for IL-2 treatment...
  36. ncbi request reprint Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34+ cells with small- and large-scale Miltenyi cell sorting system
    Claudia Voena
    Department of Haematology, Laboratory of Molecular Haematology, Istituto H S Raffaele, Milan, Italy
    Br J Haematol 117:642-5. 2002
    ..Quantitative PCR, performed in two patients, showed a tumour depletion of 1 and 2 logs respectively. Although numbers are small, the promising results obtained with small-scale selection were not reproduced in large-scale experiments...
  37. ncbi request reprint Human Herpesvirus 6 and Measles Virus Employ Distinct CD46 Domains for Receptor Function
    Heather L Greenstone
    NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:39112-8. 2002
    ..These results expand the emerging notion that CD46 and other members of the RCA family are co-opted in distinct manners by different infectious pathogens...
  38. ncbi request reprint Rational design of a CD4 mimic that inhibits HIV-1 entry and exposes cryptic neutralization epitopes
    Loic Martin
    Department of Protein Engineering and Research, CEA Saclay, 91191 Gif sur Yvette, France
    Nat Biotechnol 21:71-6. 2003
    ....
  39. ncbi request reprint Recognition of RANTES by extracellular parts of the CCR5 receptor
    Luminita Duma
    Division of Structural Biology, Biozentrum, University of Basel, Klingelbergstr 70, Basel, Switzerland
    J Mol Biol 365:1063-75. 2007
    ..These data offer new insights into the structure-function relation of the RANTES-CCR5 interaction and may be helpful for the design of novel HIV-1 inhibitors...
  40. ncbi request reprint Interaction of glycoprotein H of human herpesvirus 6 with the cellular receptor CD46
    Fabio Santoro
    Laboratory of Viral Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:25964-9. 2003
    ..e. short consensus repeats 2 and 3)...
  41. pmc Pathogenic effects of human herpesvirus 6 in human lymphoid tissue ex vivo
    Jean Charles Grivel
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:8280-9. 2003
    ....
  42. pmc Combinatorial optimization of a CD4-mimetic miniprotein and cocrystal structures with HIV-1 gp120 envelope glycoprotein
    Francois Stricher
    CEA, iBiTecS, SIMOPRO, Gif Sur Yvette F 91191, France
    J Mol Biol 382:510-24. 2008
    ....
  43. doi request reprint Pregnancy outcome in patients with pityriasis rosea
    Francesco Drago
    Department of Endocrinological and Metabolic Sciences, Section of Dermatology, University of Genoa, Genoa, Milan
    J Am Acad Dermatol 58:S78-83. 2008
    ..The effect of pityriasis rosea (PR) on the outcome of pregnancy has not been previously reported...
  44. pmc Viral interactions in human lymphoid tissue: Human herpesvirus 7 suppresses the replication of CCR5-tropic human immunodeficiency virus type 1 via CD4 modulation
    Andrea Lisco
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, NIH, Bldg 10, Rm 9D58, 9000 Rockville Pike, Bethesda, MD 20892, USA
    J Virol 81:708-17. 2007
    ....
  45. pmc Interleukin 7 reduces the levels of spontaneous apoptosis in CD4+ and CD8+ T cells from HIV-1-infected individuals
    Lia Vassena
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:2355-60. 2007
    ..These results provide a further rationale for consideration of IL-7 as an agent of immune reconstitution in HIV-1 infection...
  46. ncbi request reprint Possibility that transmissible viruses might be involved in the etiology of human cancer
    Paolo Lusso
    Rev Clin Exp Hematol 7:327-8. 2003
  47. ncbi request reprint Picking the lock with a hairpin. Did HIV-1 learn the art from chemokines?
    Paolo Lusso
    Structure 11:129-30. 2003