Roberto Burioni

Summary

Affiliation: San Raffaele Scientific Institute
Country: Italy

Publications

  1. pmc A human monoclonal antibody with neutralizing activity against highly divergent influenza subtypes
    Nicola Clementi
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italia
    PLoS ONE 6:e28001. 2011
  2. pmc A non-VH1-69 heterosubtypic neutralizing human monoclonal antibody protects mice against H1N1 and H5N1 viruses
    Donata De Marco
    Laboratory of Microbiology and Virology, Vita Salute San Raffaele University, Milan, Italy
    PLoS ONE 7:e34415. 2012
  3. ncbi request reprint Molecular cloning of the first human monoclonal antibodies neutralizing with high potency swine-origin influenza A pandemic virus (S-OIV)
    Roberto Burioni
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italy
    New Microbiol 32:319-24. 2009
  4. doi request reprint Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV)
    Roberto Burioni
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Via Olgettina 60, 20132 Milan, Italy
    Virology 399:144-52. 2010
  5. pmc Anti-HIV-1 response elicited in rabbits by anti-idiotype monoclonal antibodies mimicking the CD4-binding site
    Roberto Burioni
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Istituto Scientifico San Raffaele, Diagnostica e Ricerca San Raffaele, Milano, Italy
    PLoS ONE 3:e3423. 2008
  6. pmc Hepatitis C virus (HCV) infection may elicit neutralizing antibodies targeting epitopes conserved in all viral genotypes
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italia
    PLoS ONE 4:e8254. 2009
  7. ncbi request reprint Hepatitis C virus (HCV)-driven stimulation of subfamily-restricted natural IgM antibodies in mixed cryoglobulinemia
    Mario Perotti
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milan, Italy
    Autoimmun Rev 7:468-72. 2008
  8. ncbi request reprint Rapid molecular identification of fungal pathogens in corneal samples from suspected keratomycosis cases
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia1, Dipartimento di Oftalmologia e Scienze della Visione, IRCCS Istituto Scientifico San Raffaele, Milan, Italy
    J Med Microbiol 55:1505-9. 2006
  9. ncbi request reprint Neutralization activity and kinetics of two broad-range human monoclonal IgG1 derived from recombinant Fab fragments and directed against Hepatitis C virus E2 glycoprotein
    Roberta Antonia Diotti
    Laboratory of Microbiology and Virology, Universita Vita Salute San Raffaele, Milano, Italy
    New Microbiol 35:475-9. 2012
  10. ncbi request reprint Development and validation of a molecular method for the diagnosis of medically important fungal infections
    Donata De Marco
    Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milano
    New Microbiol 30:308-12. 2007

Detail Information

Publications36

  1. pmc A human monoclonal antibody with neutralizing activity against highly divergent influenza subtypes
    Nicola Clementi
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italia
    PLoS ONE 6:e28001. 2011
    ..A deep characterization of PN-SIA28 features may represent a useful help in the improvement of available anti-influenza therapeutic strategies and can provide new tools for the development of universal vaccinal strategies...
  2. pmc A non-VH1-69 heterosubtypic neutralizing human monoclonal antibody protects mice against H1N1 and H5N1 viruses
    Donata De Marco
    Laboratory of Microbiology and Virology, Vita Salute San Raffaele University, Milan, Italy
    PLoS ONE 7:e34415. 2012
    ..Furthermore, we describe its protective activity in mice after lethal challenge with H1N1 and H5N1 viruses suggesting a potential application in the treatment of influenza virus infections...
  3. ncbi request reprint Molecular cloning of the first human monoclonal antibodies neutralizing with high potency swine-origin influenza A pandemic virus (S-OIV)
    Roberto Burioni
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italy
    New Microbiol 32:319-24. 2009
    ..This is the first report of molecular cloning of human monoclonal antibodies against the new pandemic swine-origin influenza virus...
  4. doi request reprint Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV)
    Roberto Burioni
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Via Olgettina 60, 20132 Milan, Italy
    Virology 399:144-52. 2010
    ..Finally, the human genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered being potentially useful in the prophylaxis and the treatment of this human infection...
  5. pmc Anti-HIV-1 response elicited in rabbits by anti-idiotype monoclonal antibodies mimicking the CD4-binding site
    Roberto Burioni
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Istituto Scientifico San Raffaele, Diagnostica e Ricerca San Raffaele, Milano, Italy
    PLoS ONE 3:e3423. 2008
    ....
  6. pmc Hepatitis C virus (HCV) infection may elicit neutralizing antibodies targeting epitopes conserved in all viral genotypes
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italia
    PLoS ONE 4:e8254. 2009
    ..Their availability may open new avenues to the understanding of HCV persistence and to the development of strategies for the immune control of this infection...
  7. ncbi request reprint Hepatitis C virus (HCV)-driven stimulation of subfamily-restricted natural IgM antibodies in mixed cryoglobulinemia
    Mario Perotti
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milan, Italy
    Autoimmun Rev 7:468-72. 2008
    ..An antigen-driven stimulation of these subfamilies, and their overexpression as in HCV infection, could lead to a breaking of humoral homeostatic balance exposing the patients to the risk of developing autoimmune disorders...
  8. ncbi request reprint Rapid molecular identification of fungal pathogens in corneal samples from suspected keratomycosis cases
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia1, Dipartimento di Oftalmologia e Scienze della Visione, IRCCS Istituto Scientifico San Raffaele, Milan, Italy
    J Med Microbiol 55:1505-9. 2006
    ..Similar studies on larger panels of patients and on different clinical samples are required for further investigation of the clinical potential of ITS-based approaches in the diagnosis of mycotic infections...
  9. ncbi request reprint Neutralization activity and kinetics of two broad-range human monoclonal IgG1 derived from recombinant Fab fragments and directed against Hepatitis C virus E2 glycoprotein
    Roberta Antonia Diotti
    Laboratory of Microbiology and Virology, Universita Vita Salute San Raffaele, Milano, Italy
    New Microbiol 35:475-9. 2012
    ..This paper describes the very high neutralization activity and unique biological features of two broadly cross-reactive and cross-neutralizing anti-HCV human monoclonal IgG1 derived from human monoclonal recombinant Fab fragments...
  10. ncbi request reprint Development and validation of a molecular method for the diagnosis of medically important fungal infections
    Donata De Marco
    Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milano
    New Microbiol 30:308-12. 2007
    ....
  11. pmc Cross-reacting antibacterial auto-antibodies are produced within coronary atherosclerotic plaques of acute coronary syndrome patients
    Filippo Canducci
    Laboratory of Microbiology and Virology, Ospedale San Raffaele, Milan, Italy
    PLoS ONE 7:e42283. 2012
    ..These findings demonstrated that in human atherosclerotic plaques a local cross-reactive immune response takes place...
  12. ncbi request reprint Cloning and molecular characterization of a human recombinant IgG Fab binding to the Tat protein of human immunodeficiency virus type 1 (HIV-1) derived from the repertoire of a seronegative patient
    Nicasio Mancini
    Universita Vita Salute San Raffaele, San Raffaele Diagnostica e Ricerca, IRCCS Istituto Scientifico San Raffaele, Via Olgettina 60, 20132 Milano, Italy
    Mol Immunol 43:1363-9. 2006
    ....
  13. doi request reprint Anti-hepatitis C virus E2 (HCV/E2) glycoprotein monoclonal antibodies and neutralization interference
    Giuseppe Sautto
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italy
    Antiviral Res 96:82-9. 2012
    ..Overall, a better comprehension of this phenomenon may be of importance in the set up of novel mAb-based anti-HCV therapeutic strategies...
  14. pmc Dynamic features of the selective pressure on the human immunodeficiency virus type 1 (HIV-1) gp120 CD4-binding site in a group of long term non progressor (LTNP) subjects
    Filippo Canducci
    Laboratorio di Microbiologia e Virologa, Universita Vita Salute San Raffaele, Milan, Italy
    Retrovirology 6:4. 2009
    ..Overall, the data shown here documenting high selective pressure on the HIV-1 CD4bs of a group of LTNP subjects offers important insights for planning novel strategies for the immune control of HIV-1 infection...
  15. pmc Influenza B-cells protective epitope characterization: a passkey for the rational design of new broad-range anti-influenza vaccines
    Nicola Clementi
    Microbiology and Virology Unit, Vita Salute San Raffaele University, Milan 20132, Italy
    Viruses 4:3090-108. 2012
    ....
  16. ncbi request reprint A phage display vector optimized for the generation of human antibody combinatorial libraries and the molecular cloning of monoclonal antibody fragments
    Laura Solforosi
    Laboratory of Microbiology and Virology, Vita Salute San Raffaele University, Milan, Italy
    New Microbiol 35:289-94. 2012
    ..The phagemid pCM was used to generate a human combinatorial phage display antibody library that allowed the selection of a monoclonal Fab fragment antibody directed against the nucleoprotein (NP) of Influenza A virus...
  17. pmc HCV proteins and immunoglobulin variable gene (IgV) subfamilies in HCV-induced type II mixed cryoglobulinemia: a concurrent pathogenetic role
    Giuseppe Sautto
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Via Olgettina, 58, 20132 Milano, Italy
    Clin Dev Immunol 2012:705013. 2012
    ..In this paper, we analyze both the humoral and viral counterparts at the basis of cryoglobulins production in HCV-induced MCII, with particular attention reserved to the single IgV subfamilies most frequently involved...
  18. pmc Identification of a broadly cross-reacting and neutralizing human monoclonal antibody directed against the hepatitis C virus E2 protein
    Mario Perotti
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, DIBIT2, Via Olgettina 60, 20132 Milano, Italia
    J Virol 82:1047-52. 2008
    ..Fab e137 was also able to inhibit cell culture-grown HCV (genotype 2a). These data indicate that broadly cross-reacting and cross-neutralizing antibodies are generated during HCV infection...
  19. ncbi request reprint Modulation of epitope-specific anti-hepatitis C virus E2 (anti-HCV/E2) antibodies by anti-viral treatment
    Nicasio Mancini
    Laboratorio di Microbiologia, Universita Vita Salute San Raffaele, Istituto Scientifico San Raffaele, Milano, Italy
    J Med Virol 78:1304-11. 2006
    ....
  20. doi request reprint A potential role for monoclonal antibodies in prophylactic and therapeutic treatment of influenza
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italy
    Antiviral Res 92:15-26. 2011
    ..In this review, all major monoclonal antibodies directed against different influenza virus proteins are reported and their potential in the design of new anti-influenza prophylactic or therapeutic strategies is discussed...
  21. doi request reprint Antigen-driven evolution of B lymphocytes in coronary atherosclerotic plaques
    Roberto Burioni
    Universita Vita Salute San Raffaele, Milano, Italy
    J Immunol 183:2537-44. 2009
    ..The data document for the first time the presence of a local Ag-driven clonal evolution of B cells in human atherosclerotic plaques...
  22. ncbi request reprint Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction
    Mario Perotti
    Università Vita Salute San Raffaele and Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milan, Italy
    J Microbiol Methods 65:632-6. 2006
    ..The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo...
  23. doi request reprint Characterization of epitopes recognized by monoclonal antibodies: experimental approaches supported by freely accessible bioinformatic tools
    Nicola Clementi
    Microbiology and Virology Unit, Vita Salute San Raffaele University, 20132 Milan, Italy Electronic address
    Drug Discov Today 18:464-71. 2013
    ..In this article, we review the most common, freely accessible, bioinformatic tools used for epitope characterization and provide some basic examples of molecular visualization, editing and computational analysis...
  24. pmc Molecular signatures of hepatitis C virus (HCV)-induced type II mixed cryoglobulinemia (MCII)
    Giuseppe Sautto
    Microbiology and Virology Unit, Vita Salute San Raffaele University, Via Olgettina 58, Milan 20132, Italy
    Viruses 4:2924-44. 2012
    ..In this review, we will analyze the host and viral factors taking part in the development of MCII in order to give a general outlook of the molecular mechanisms implicated...
  25. pmc The era of molecular and other non-culture-based methods in diagnosis of sepsis
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Diagnostica e Ricerca San Raffaele, Milano, Italia
    Clin Microbiol Rev 23:235-51. 2010
    ..In particular, the potential clinical role for the sensitive and rapid detection of bacterial and fungal DNA in the development of new diagnostic algorithms is discussed...
  26. pmc Neutralization interfering antibodies: a "novel" example of humoral immune dysfunction facilitating viral escape?
    Mancini Nicasio
    Microbiology and Virology Unit, Vita Salute San Raffaele University, Via Olgettina 58, Milan 20132, Italy
    Viruses 4:1731-52. 2012
    ..In this review, we illustrate the mechanisms of Ab-mediated interference and the viral pathogens described in literature as able to adopt this "novel" evasion strategy...
  27. pmc Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milan, Italy
    J Clin Microbiol 52:1242-5. 2014
    ..7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively. ..
  28. doi request reprint Potential role of the detection of enterobacterial DNA in blood for the management of neonatal necrotizing enterocolitis
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italy
    J Med Microbiol 61:1465-72. 2012
    ....
  29. doi request reprint Epitope mapping by epitope excision, hydrogen/deuterium exchange, and peptide-panning techniques combined with in silico analysis
    Nicola Clementi
    Microbiology and Virology Unit, Vita Salute San Raffaele University, Milan, Italy
    Methods Mol Biol 1131:427-46. 2014
    ..Here, we review few experimental techniques followed by the use of "basic" informatics tools for the analysis of the results...
  30. pmc Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
    Beatrice Barda
    Laboratory of Microbiology San Raffaele Hospital, Milan, Italy
    Parasit Vectors 7:271. 2014
    ..The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina...
  31. pmc Comparative evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milan, Italy
    J Clin Microbiol 51:2453-7. 2013
    ..3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036)...
  32. doi request reprint Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies
    Nicasio Mancini
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italy
    J Med Microbiol 57:601-4. 2008
    ..Overall, the characteristics featured by the molecular method could be of interest in the development of new algorithms for the diagnosis of sepsis in critical patients...
  33. ncbi request reprint Cost-effectiveness of blood culture and a multiplex real-time PCR in hematological patients with suspected sepsis: an observational propensity score-matched study
    Nicasio Mancini
    Laboratory of Microbiology and Virology, University Vita Salute San Raffaele, Milan, Italy
    Expert Rev Mol Diagn 14:623-32. 2014
    ..The apparently more expensive molecular assay favored a more rational use of economic resources without influencing, and probably improving, the clinical outcome. ..
  34. pmc Possible future monoclonal antibody (mAb)-based therapy against arbovirus infections
    Giuseppe Sautto
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, 20132 Milano, Italy
    Biomed Res Int 2013:838491. 2013
    ..In this review are described the main arboviruses infecting humans and candidate mAbs to be possibly used in a future passive immunotherapy. ..
  35. pmc Mini-FLOTAC and Kato-Katz: helminth eggs watching on the shore of Lake Victoria
    Beatrice Barda
    Laboratory of Microbiology San Raffaele Hospital, Milan, Italy
    Parasit Vectors 6:220. 2013
    ..The aim of our study was to evaluate and compare, the WHO recommended quantitative diagnostic technique (Kato-Katz) and the Mini-FLOTAC...
  36. ncbi request reprint Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis
    Nicasio Mancini
    Laboratorio di Microbiologia, Diagnostica e Ricerca San Raffaele and Centro Malattie della Cornea, Dipartimento di Oftalmologia e Scienze della Visione, IRCCS Istituto Scientifico San Raffaele, Via Olgettina 60, 20132 Milano, Italy
    J Med Microbiol 54:897-900. 2005
    ..The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology...