Research Topics
Genomes and Genes
| Marco E BianchiSummaryAffiliation: San Raffaele Scientific Institute Country: Italy Publications
| Collaborators
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Detail Information
Publications
Requirement of HMGB1 and RAGE for the maturation of human plasmacytoid dendritic cellsIngrid E Dumitriu
H San Raffaele Scientific Institute, Milan, Italy
Eur J Immunol 35:2184-90. 2005..These results reveal HMGB1 and RAGE as the first known autocrine loop modulating the maturation of PDC, and suggest that antagonists of HMGB1/RAGE might have therapeutic potential for the treatment of systemic human diseases...- DAMPs, PAMPs and alarmins: all we need to know about dangerMarco E Bianchi
San Raffaele University, Chromatin Dynamics Unit, Via Olgettina 58, 20132 Milan, Italy
J Leukoc Biol 81:1-5. 2007..Endogenous alarmins and exogenous PAMPs therefore convey a similar message and elicit similar responses; they can be considered subgroups of a larger set, the damage-associated molecular patterns (DAMPs)... - HMG proteins: dynamic players in gene regulation and differentiationMarco E Bianchi
Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milano, Italy
Curr Opin Genet Dev 15:496-506. 2005..Moreover, they contribute to the fine tuning of transcription in response to rapid environmental changes. They do so by interacting with nucleosomes, transcription factors, nucleosome-remodelling machines, and with histone H1... - High-mobility group box 1 (HMGB1) protein at the crossroads between innate and adaptive immunityMarco E Bianchi
Faculty of Medicine, San Raffaele University, Milano, Italy
Immunol Rev 220:35-46. 2007..These immune responses will also be directed against self-antigens that DCs process at the time of injury and can lead to autoimmunity... - Significant (re)location: how to use chromatin and/or abundant proteins as messages of life and deathMarco E Bianchi
San Raffaele University, Via Olgettina 58, 20132 Milan, Italy
Trends Cell Biol 14:287-93. 2004 - Chromatin and cell deathMarco E Bianchi
San Raffaele Scientific Institute, Via Olgettina 58, 4 Piano A1, Milan I 20132, Italy
Biochim Biophys Acta 1677:181-6. 2004..Thus, dying cells use their own chromatin to signal how they have died. We argue that the nuclear events in apoptosis serve to control the molecular signals that dying cells send out... 
HMGB1 loves companyMarco E Bianchi
San Raffaele University and San Raffaele Research Institute, Department of Genetics and Cell Biology, 20132 Milano, Italy
J Leukoc Biol 86:573-6. 2009..Thus, HMGB1 has dual activities, solo or in company; I speculate that this may serve our body's necessity to sacrifice or reconstruct tissues as required by the presence or absence of pathogens...- Ancient news: HMGBs are universal sentinelsMarco E Bianchi
Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele University and Scientific Institute, Via Olgettina, 58, 20132 Milan, Italy
J Mol Cell Biol 2:116-7. 2010..This appears to be an evolutionary ancient mechanism of virus detection, and possibly might be a facet of a more general propensity of HMGBs to act as integrators of signals that pertain to peace and stress, life and death... 
Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine releaseEmilie Venereau
Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milan, Italy
J Exp Med 209:1519-28. 2012..Thus, HMGB1 orchestrates both key events in sterile inflammation, leukocyte recruitment and their induction to secrete inflammatory cytokines, by adopting mutually exclusive redox states...- Inhibitor of NF-kappa B kinases alpha and beta are both essential for high mobility group box 1-mediated chemotaxis [corrected]Marianna Penzo
Vita Salute San Raffaele University, School of Medicine, San Raffaele Scientific Institute, Milano, Italy
J Immunol 184:4497-509. 2010..Thus, proinflammatory HMGB1 chemotactic responses mechanistically require the differential collaboration of both IKK-dependent NF-kappaB signaling pathways... - Redox remodeling: a candidate regulator of HMGB1 function in injured skeletal muscleMichela Vezzoli
Innate Immunity and Tissue Remodeling Unit, San Raffaele Scientific Institute, Milano, Italy
Ann N Y Acad Sci 1209:83-90. 2010..Moreover, data suggest a potential role for infiltrating alternatively activated macrophages to influence the outcome of inflammatory responses to sterile skeletal muscle necrosis... - The secretion of HMGB1 is required for the migration of maturing dendritic cellsIngrid E Dumitriu
Clinical Immunology Unit, H San Raffaele Scientific Institute and Università Vita Salute San Raffaele, Via Olgettina 58, Milano 20132, Italy
J Leukoc Biol 81:84-91. 2007..Our data suggest that the autocrine/paracrine release of HMGB1 and the integrity of the HMGB1/RAGE pathway are required for the migratory function of DC... - HMGB1: guiding immunity from withinIngrid E Dumitriu
Cancer Immunotherapy and Gene Therapy Program, Clinical Immunology Unit, H San Raffaele Scientific Institute, Via Olgettina 58, Milano 20132, Italy
Trends Immunol 26:381-7. 2005..The role of HMGB1 as an endogenous molecule that facilitates immune responses and has an important role in tissue homeostasis and disease will be highlighted here... - Src family kinases are necessary for cell migration induced by extracellular HMGB1Roberta Palumbo
San Raffaele Research Institute, 20132 Milano, Italy
J Leukoc Biol 86:617-23. 2009..The ablation of Src or inhibition with the kinase inhibitor PP2 blocks migration toward HMGB1. Src associates to and mediates the phosphorylation of FAK and the formation of focal adhesions... - Inflammatory and alternatively activated human macrophages attract vessel-associated stem cells, relying on separate HMGB1- and MMP-9-dependent pathwaysKarine Lolmede
Clinical Cardiovascular Biology Research Center, H San Raffaele Scientific Institute, Milano, Italy
J Leukoc Biol 85:779-87. 2009..Moreover, they open the possibility of novel strategies, aimed at interfering selectively with signals that recruit blood-derived stem cells toward pro- or anti-inflammatory macrophages... - Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-kappaB activationRoberta Palumbo
Chromatin Dynamics Unit, Stem Cell Research Institute, Istituto Scientifico San Raffaele, 20132 Milan, Italy
J Cell Biol 179:33-40. 2007..These findings suggest that NF-kappaB signaling controls tissue regeneration in addition to early events in inflammation... - Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretionTiziana Bonaldi
DIBIT, San Raffaele Scientific Institute, San Raffaele University, Via Olgettina 58, 20132 Milan, Italy
EMBO J 22:5551-60. 2003..Cytosolic HMGB1 is then concentrated by default into secretory lysosomes, and secreted when monocytic cells receive an appropriate second signal... - High mobility group B2 is secreted by myeloid cells and has mitogenic and chemoattractant activities similar to high mobility group B1Tobias Pusterla
San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, Italy
Autoimmunity 42:308-10. 2009..Since extracellular HMGB2 has been detected in the blood and other biological fluids, it might be necessary to target HMGB2 at the same time as HMGB1 for therapeutical efficacy... - Treatment with HMGB1 inhibitors diminishes CTL-induced liver disease in HBV transgenic miceGiovanni Sitia
San Raffaele Scientific Institute, Chromatin Dynamics Unit, Via Olgettina 58, Milan 20132, Italy
J Leukoc Biol 81:100-7. 2007.... - GR and HMGB1 interact only within chromatin and influence each other's residence timeAlessandra Agresti
San Raffaele Research Institute, Via Olgettina 58, 20132 Milan, Italy
Mol Cell 18:109-21. 2005..We propose that kinetic cooperativity among transcription factors in chromatin binding may be a common feature in transcription and DNA transactions... - Substantial histone reduction modulates genomewide nucleosomal occupancy and global transcriptional outputBarbara Celona
San Raffaele University, Milan, Italy
PLoS Biol 9:e1001086. 2011..We suggest that variation in nucleosome number, by affecting nucleosomal occupancy both genomewide and gene-specifically, constitutes a novel layer of epigenetic regulation... - Regulation of dendritic- and T-cell fate by injury-associated endogenous signalsAngelo A Manfredi
Vita Salute San Raffaele University and San Raffaele Scientific Institute, 20132 Milano, Italy
Crit Rev Immunol 29:69-86. 2009.... - HMGB1: a signal of necrosisAngela Raucci
San Raffaele Scientific Institute, Milan, Italy
Autoimmunity 40:285-9. 2007..Because of its powerful activities, HMGB1 is involved in several disorders, including autoimmune ones... - Glycyrrhizin binds to high-mobility group box 1 protein and inhibits its cytokine activitiesLuca Mollica
Biomolecular NMR Laboratory, Dulbecco Telethon Institute, San Raffaele Scientific Institute, Via Olgettina 58, 20133 Milan, Italy
Chem Biol 14:431-41. 2007..Our results explain in part the anti-inflammatory properties of glycyrrhizin, and might direct the design of new derivatives with improved HMGB1-binding properties... - Kupffer cells hasten resolution of liver immunopathology in mouse models of viral hepatitisGiovanni Sitia
Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy
PLoS Pathog 7:e1002061. 2011..Overall, these results indicate that KCs resolve rather than worsen liver immunopathology... - Smooth muscle cells in human atherosclerotic plaques secrete and proliferate in response to high mobility group box 1 proteinAnnalisa Porto
San Raffaele Scientific Institute, Milan, Italy
FASEB J 20:2565-6. 2006..Thus, SMCs are both a source and a target of HMGB1; blocking HMGB1 secretion by SMCs can be an important strategy for treatment of atherosclerotic disease and in particular restenosis... - HMGB1 is an endogenous immune adjuvant released by necrotic cellsPatrizia Rovere-Querini
Cancer Immunotherapy and Gene Therapy Program, Clinical Immunology Unit H San Raffaele Scientific Institute and Vita Salute San Raffaele University, DIBIT 3A1, Chromatin Dynamics Unit, Via Olgettina 58, 20132 Milano, Italy
EMBO Rep 5:825-30. 2004..In vivo, HMGB1 enhances the primary antibody responses to soluble antigens and transforms poorly immunogenic apoptotic lymphoma cells into efficient vaccines... - HMGB proteins and gene expressionAlessandra Agresti
DIBIT, Istituto Scientifico San Raffaele, Via Olgettina 58, 20132 Milano, Italy
Curr Opin Genet Dev 13:170-8. 2003..Similarly, HMGBs interact with nucleosomes and promote their sliding, but remain bound only for fractions of a second. We argue that HMGBs fluidize chromatin - an action that appears opposite to that of histone H1... - Release of chromatin protein HMGB1 by necrotic cells triggers inflammationPaola Scaffidi
DIBIT, Istituto Scientifico San Raffaele, 20132 Milano, Italy
Nature 418:191-5. 2002..Thus, cells undergoing apoptosis are programmed to withhold the signal that is broadcast by cells that have been damaged or killed by trauma... - TLR4-mediated skin carcinogenesis is dependent on immune and radioresistant cellsDeepak Mittal
Department of Experimental Oncology, European Institute of Oncology, Milano, Italy
EMBO J 29:2242-52. 2010..Together, our results suggest that the initial release of HMGB1 triggers a TLR4-dependent inflammatory response that leads to tumour development... - Maturing dendritic cells depend on RAGE for in vivo homing to lymph nodesAngelo A Manfredi
Clinical Immunology Unit, H San Raffaele Scientific Institute and Università Vita Salute San Raffaele, Via Olgettina 48, Milan, Italy
J Immunol 180:2270-5. 2008..Thus the HMGB1-RAGE pathway is a checkpoint in DC maturation and function and a candidate for targeted therapies... - HMGB1 interacts differentially with members of the Rel family of transcription factorsAlessandra Agresti
DIBIT, San Raffaele Scientific Institute, Milan, Italy
Biochem Biophys Res Commun 302:421-6. 2003..Functionally, HMGB1 is required for the NF-kappaB-dependent expression of the adhesion molecule VCAM-1... - A soluble form of the receptor for advanced glycation endproducts (RAGE) is produced by proteolytic cleavage of the membrane-bound form by the sheddase a disintegrin and metalloprotease 10 (ADAM10)Angela Raucci
San Raffaele Scientific Institute, Milan, Italy
FASEB J 22:3716-27. 2008..Our data do not disprove the interpretation that high levels of soluble forms of RAGE protect against chronic inflammation, but rather suggest that they correlate with high levels of ongoing inflammation... - The evolution of High Mobility Group Box (HMGB) chromatin proteins in multicellular animalsLuca Sessa
Vita Salute San Raffaele University, Chromatin Dynamics Unit, Via Olgettina 58, 20132 Milano, Italy
Gene 387:133-40. 2007..The organization of HMGB genes was very conserved during Metazoan evolution, with the only deviations appearing in Caenorhabditis and Dipteran (Drosophila and Anopheles) species... - HMGB1 and leukocyte migration during trauma and sterile inflammationEmilie Venereau
San Raffaele University and Scientific Institute, Milan, Italy
Mol Immunol 55:76-82. 2013..The HMGB1-CXCL12 heterocomplex constitutes a specific target that may hold promise for the treatment of several pathologies... - Toll-like receptor 4 and high-mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizuresMattia Maroso
Department of Neuroscience, Mario Negri Institute for Pharmacological Research, Milano, Italy
Nat Med 16:413-9. 2010..Thus, HMGB1-TLR4 signaling may contribute to generating and perpetuating seizures in humans and might be targeted to attain anticonvulsant effects in epilepsies that are currently resistant to drugs... - HMGB1, an architectural chromatin protein and extracellular signalling factor, has a spatially and temporally restricted expression pattern in mouse brainStefania Guazzi
DIBIT, San Raffaele Scientific Institute, Via Olgettina, 58, 20132 Milan, Italy
Gene Expr Patterns 3:29-33. 2003..Finally, HMGB1 subcellular localization changes during retinoic acid induced differentiation of P19 neuroblastoma cells... - High mobility group box 1 protein, a cue for stem cell recruitmentRoberta Palumbo
Department of Molecular Biology and Functional Genomics, San Raffaele Research Institute, San Raffaele University, Via Olgettina 58, 20132 Milan, Italy
Biochem Pharmacol 68:1165-70. 2004..The inflammatory and tissue-regenerating roles of HMGB1 may be strictly interconnected, and are discussed here... - Yeast Nhp6A/B and mammalian Hmgb1 facilitate the maintenance of genome stabilitySabrina Giavara
The Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
Curr Biol 15:68-72. 2005..Taken together, these data indicate that Nhp6A/B and Hmgb1 protect DNA from damaging agents and thus guard against the generation of genomic aberrations... - The nuclear protein HMGB1 is secreted by monocytes via a non-classical, vesicle-mediated secretory pathwayStefania Gardella
National Cancer Research Institute, Genoa, Italy
EMBO Rep 3:995-1001. 2002..Thus, in monocytes, non-classical secretion can occur through vescicle compartments that are at least partially distinct... - The DNA chaperone HMGB1 facilitates ACF/CHRAC-dependent nucleosome slidingTiziana Bonaldi
Adolf Butenandt Institut, Molekularbiologie, Schillerstrasse 44, D 80336 MUnchen, Germany
EMBO J 21:6865-73. 2002..By contrast, an HMGB1 mutant with increased binding affinity was inhibitory. These observations are consistent with a role for HMGB1 as a DNA chaperone facilitating the rate-limiting DNA distortion during nucleosome remodelling... - Exogenous high-mobility group box 1 protein induces myocardial regeneration after infarction via enhanced cardiac C-kit+ cell proliferation and differentiationFederica Limana
Laboratorio di Patologia Vascolare, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy
Circ Res 97:e73-83. 2005..Thus, HMGB1 appears to be a potent inducer of myocardial regeneration following myocardial infarction... - A novel pathway of HMGB1-mediated inflammatory cell recruitment that requires Mac-1-integrinValeria V Orlova
Experimental Immunology Branch, NCI, NIH, Bethesda, MD 20892, USA
EMBO J 26:1129-39. 2007..Together, a novel HMGB1-dependent pathway for inflammatory cell recruitment and activation that requires the functional interplay between Mac-1 and RAGE is described here... - Release of high mobility group box 1 by dendritic cells controls T cell activation via the receptor for advanced glycation end productsIngrid E Dumitriu
Cancer Immunotherapy and Gene Therapy Program, Clinical Immunology Unit, H San Raffaele Scientific Institute, Milan, Italy
J Immunol 174:7506-15. 2005..The use of an ancient signal of necrosis, HMGB1, by dendritic cells to sustain their own maturation and for activation of T lymphocytes represents a profitable evolutionary mechanism... - Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferationRoberta Palumbo
Department of Molecular Biology and Functional Genomics, San Raffaele Research Institute, Milan, Italy
J Cell Biol 164:441-9. 2004..Although the role of endogenous HMGB1 in the reconstruction of dystrophic muscle remains to be clarified, injected HMGB1 may be used to promote tissue regeneration... - A hyper-dynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-kappaB-dependent gene activityDaniela Bosisio
Institute for Research in Biomedicine, Bellinzona, Switzerland
EMBO J 25:798-810. 2006..We propose a revision of the enhanceosome concept in this dynamic framework... - Stage-specific secretion of HMGB1 in cartilage regulates endochondral ossificationNoboru Taniguchi
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Cell Biol 27:5650-63. 2007..Collectively, these findings suggest that HMGB1 released from differentiating chondrocytes acts, at least in part, as a regulator of endochondral ossification during osteogenesis... - A novel role for HMGB1 in TLR9-mediated inflammatory responses to CpG-DNAStanimir Ivanov
Department of Molecular Microbiology and Immunology, Brown University, Providence, RI 02912, USA
Blood 110:1970-81. 2007..However, lack of intracellular TLR9-associated HMGB1 can be compensated by extracellular HMGB1. Thus, the DNA-binding protein HMGB1 shuttles in and out of immune cells and regulates inflammatory responses to CpG-DNA... - High-mobility group box-1 in ischemia-reperfusion injury of the heartMartin Andrassy
Department of Medicine III, University of Heidelberg, Heidelberg, Germany
Circulation 117:3216-26. 2008..Although HMGB1 has been implicated in ischemia/reperfusion (I/R) injury of the liver and lung, its role in I/R injury of the heart remains unclear... - High-mobility group box 1 protein in human and murine skin: involvement in wound healingStefania Straino
Laboratorio di Patologia Vascolare, Istituto Dermopatico dell Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy
J Invest Dermatol 128:1545-53. 2008..In conclusion, lower HMGB1 levels in diabetic skin may play an important role in impaired wound healing and this defect may be overcome by the topical application of HMGB1... - The long acidic tail of high mobility group box 1 (HMGB1) protein forms an extended and flexible structure that interacts with specific residues within and between the HMG boxesStefan Knapp
Discovery Research Oncology, Department of Chemistry, Pharmacia Corporation, Viale Pasteur 10, 20014 Nerviano, Italy
Biochemistry 43:11992-7. 2004..The tail interacts with specific residues in the boxes and shields them from other interactions... - Sexy splicing: regulatory interplays governing sex determination from Drosophila to mammalsEnzo Lalli
Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Universite Louis Pasteur, B P 163, 67404 Illkirch, Strasbourg, France
J Cell Sci 116:441-5. 2003..The recent identification of a role for the key regulatory factors SRY and WT1(+KTS) in pre-mRNA splicing indicates that important steps in the mammalian sex determination process are likely to operate at the post-transcriptional level... - A nuclear protein complex containing high mobility group proteins B1 and B2, heat shock cognate protein 70, ERp60, and glyceraldehyde-3-phosphate dehydrogenase is involved in the cytotoxic response to DNA modified by incorporation of anticancer nucleosideEugene Y Krynetski
Department of Pharmaceutical Sciences, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Cancer Res 63:100-6. 2003.... - Multiple effects of high mobility group box protein 1 in skeletal muscle regenerationRoberta De Mori
Laboratorio di Patologia Vascolare, Istituto Dermopatico dell Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy
Arterioscler Thromb Vasc Biol 27:2377-83. 2007..High mobility group box 1 protein (HMGB1) is a cytokine released by necrotic and inflammatory cells in response to injury. We examined the role of HMGB1 in skeletal muscle regeneration after hindlimb ischemia... - High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cellsEmmanouil Chavakis
Molecular Cardiology, Department of Internal Medicine III, J W Goethe University of Frankfurt, Frankfurt, Germany
Circ Res 100:204-12. 2007..These results may provide a platform for the development of novel therapeutic approaches to improve EPC homing... - Association of chromatin proteins high mobility group box (HMGB) 1 and HMGB2 with mitotic chromosomesCoralie Pallier
Unité de virologie, Service de Bacteriologie Virologie, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, 94275 Le Kremlin Bicetre, France
Mol Biol Cell 14:3414-26. 2003..A comparable behavior was also observed for two proteins of the HMG-nucleosome binding (HMGN) group, namely, HMGN1 and HMGN2... - Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 ruleDennis J Sawchuk
Laboratory of Molecular Immunology and Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
J Biol Chem 279:29821-31. 2004....