Claudio Dalvit

Summary

Affiliation: Nerviano Medical Sciences
Country: Italy

Publications

  1. ncbi NMR-based quality control approach for the identification of false positives and false negatives in high throughput screening
    Claudio Dalvit
    Chemistry Department Nerviano Medical Sciences, Viale Pasteur 10, 20014 Nerviano, Milan, Italy
    Curr Drug Discov Technol 3:115-24. 2006
  2. ncbi Sensitivity improvement in 19F NMR-based screening experiments: theoretical considerations and experimental applications
    Claudio Dalvit
    Chemistry Department, Nerviano Medical Sciences, 20014 Nerviano, Milano, Italy
    J Am Chem Soc 127:13380-5. 2005
  3. ncbi Reliable high-throughput functional screening with 3-FABS
    Claudio Dalvit
    Chemistry Department, Nerviano Medical Science, Viale Pasteur 10, 20014 Nerviano MI, Italy
    Drug Discov Today 9:595-602. 2004
  4. ncbi Competition binding experiments for rapidly ranking lead molecules for their binding affinity to human serum albumin
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:645-50. 2002
  5. ncbi Polyfluorinated amino acids for sensitive 19F NMR-based screening and kinetic measurements
    Gianluca Papeo
    Chemistry Department, Nerviano Medical Sciences, Viale Pasteur 10, 20014 Nerviano, Milano, Italy
    J Am Chem Soc 129:5665-72. 2007
  6. ncbi A general NMR method for rapid, efficient, and reliable biochemical screening
    Claudio Dalvit
    Chemistry and Biology Departments, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Am Chem Soc 125:14620-5. 2003
  7. ncbi High-throughput NMR-based screening with competition binding experiments
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Milan, Italy
    J Am Chem Soc 124:7702-9. 2002
  8. ncbi Fluorine-NMR competition binding experiments for high-throughput screening of large compound mixtures
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:605-11. 2002
  9. ncbi Fluorine-NMR experiments for high-throughput screening: theoretical aspects, practical considerations, and range of applicability
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Am Chem Soc 125:7696-703. 2003
  10. ncbi Inhibition of protein-protein interactions: the discovery of druglike beta-catenin inhibitors by combining virtual and biophysical screening
    Jean Yves Trosset
    Department of Chemistry, Nerviano Medical Sciences, Nerviano, MI, Italy
    Proteins 64:60-7. 2006

Detail Information

Publications12

  1. ncbi NMR-based quality control approach for the identification of false positives and false negatives in high throughput screening
    Claudio Dalvit
    Chemistry Department Nerviano Medical Sciences, Viale Pasteur 10, 20014 Nerviano, Milan, Italy
    Curr Drug Discov Technol 3:115-24. 2006
    ..Examples of this approach in the identification of false positives in a screening run against the enzyme thymidine phosphorylase (TP) and the rescue of a false negative in a screening run against the Ser/Thr kinase AKT1 are presented...
  2. ncbi Sensitivity improvement in 19F NMR-based screening experiments: theoretical considerations and experimental applications
    Claudio Dalvit
    Chemistry Department, Nerviano Medical Sciences, 20014 Nerviano, Milano, Italy
    J Am Chem Soc 127:13380-5. 2005
    ..Application of 3-FABS combined with the cryogenic (19)F probe technology to rapid screening at very low enzyme concentrations and the current detection limits reached with this approach are also presented...
  3. ncbi Reliable high-throughput functional screening with 3-FABS
    Claudio Dalvit
    Chemistry Department, Nerviano Medical Science, Viale Pasteur 10, 20014 Nerviano MI, Italy
    Drug Discov Today 9:595-602. 2004
    ....
  4. ncbi Competition binding experiments for rapidly ranking lead molecules for their binding affinity to human serum albumin
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:645-50. 2002
    ..This review will summarize some of the NMR-based efforts towards developing universal, rapid, accurate, and site-specific assays for estimating protein binding...
  5. ncbi Polyfluorinated amino acids for sensitive 19F NMR-based screening and kinetic measurements
    Gianluca Papeo
    Chemistry Department, Nerviano Medical Sciences, Viale Pasteur 10, 20014 Nerviano, Milano, Italy
    J Am Chem Soc 129:5665-72. 2007
    ....
  6. ncbi A general NMR method for rapid, efficient, and reliable biochemical screening
    Claudio Dalvit
    Chemistry and Biology Departments, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Am Chem Soc 125:14620-5. 2003
    ..Applications of this technique to the screening of inhibitors of the Ser/Thr kinase AKT1 and the protease trypsin are presented. In addition, an interesting application of 3-FABS to functional genomics is also presented...
  7. ncbi High-throughput NMR-based screening with competition binding experiments
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Milan, Italy
    J Am Chem Soc 124:7702-9. 2002
    ..With this approach it is possible to screen thousands of compounds in a short period of time against protein or DNA and RNA fragments. The methodology can also be applied for screening plant and fungi extracts...
  8. ncbi Fluorine-NMR competition binding experiments for high-throughput screening of large compound mixtures
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, Nerviano MI, 20014, Italy
    Comb Chem High Throughput Screen 5:605-11. 2002
    ..Finally, a novel approach for HTS using a reference molecule in combination with a control molecule is presented...
  9. ncbi Fluorine-NMR experiments for high-throughput screening: theoretical aspects, practical considerations, and range of applicability
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Am Chem Soc 125:7696-703. 2003
    ..The experimental conditions together with the detection limits and binding constant measurements are investigated using human serum albumin as the target...
  10. ncbi Inhibition of protein-protein interactions: the discovery of druglike beta-catenin inhibitors by combining virtual and biophysical screening
    Jean Yves Trosset
    Department of Chemistry, Nerviano Medical Sciences, Nerviano, MI, Italy
    Proteins 64:60-7. 2006
    ....
  11. ncbi Multi-selective one dimensional proton NMR experiments for rapid screening and binding affinity measurements
    Claudio Dalvit
    Chemistry Department, Pharmacia, Viale Pasteur 10, 20014 Nerviano, MI, Italy
    Comb Chem High Throughput Screen 6:445-53. 2003
    ..For a simple competition binding mechanism, the rapidly-derived NMR binding constants are in good agreement with the values derived from full-titration ITC and fluorescence spectroscopy measurements...
  12. ncbi NMR-Based screening with competition water-ligand observed via gradient spectroscopy experiments: detection of high-affinity ligands
    Claudio Dalvit
    Department of Chemistry, Pharmacia, Viale Pasteur 10, 20014 Nerviano MI, Italy
    J Med Chem 45:2610-4. 2002
    ..The technique is particularly suitable for rapid screening of chemical mixtures and plant or fungi extracts...