Franco Locatelli

Summary

Country: Italy

Publications

  1. ncbi request reprint Natural killer cells in the treatment of high-risk acute leukaemia
    Franco Locatelli
    Department of Paediatric Haematology and Oncology, IRCCS Bambino Gesù Children s Hospital, Rome, Italy University of Pavia, Pavia, Italy Electronic address
    Semin Immunol 26:173-9. 2014
  2. doi request reprint Current and future approaches to treat graft failure after allogeneic hematopoietic stem cell transplantation
    Franco Locatelli
    IRCCS Ospedale Pediatrico Bambino Gesù, Department of Pediatric Oncology and Hematology, Piazza Sant Onofrio 4, 00165 Rome, Italy 39 06 68592678 39 06 68592292
    Expert Opin Pharmacother 15:23-36. 2014
  3. ncbi request reprint Negative depletion of α/β+ T cells and of CD19+ B lymphocytes: a novel frontier to optimize the effect of innate immunity in HLA-mismatched hematopoietic stem cell transplantation
    Franco Locatelli
    Department of Pediatric Hematology and Oncology, IRCCS Ospedale Bambino Gesù, Rome, Italy University of Pavia, Italy Electronic address
    Immunol Lett 155:21-3. 2013
  4. doi request reprint At the Bedside: Innate immunity as an immunotherapy tool for hematological malignancies
    Franco Locatelli
    1 Piazza Sant Onofrio 4, 00165 Rome, Italy
    J Leukoc Biol 94:1141-57. 2013
  5. doi request reprint Outcome of patients with hemoglobinopathies given either cord blood or bone marrow transplantation from an HLA-identical sibling
    Franco Locatelli
    Department of Pediatric Hematology Oncology, Istituto di Ricovero e Cura a Carattere Scientifico, Ospedale Pediatrico Bambino Gesu, Piazzale Sant Onofrio 4, Rome, Italy
    Blood 122:1072-8. 2013
  6. pmc ADAR2 editing activity in newly diagnosed versus relapsed pediatric high-grade astrocytomas
    Sara Tomaselli
    Laboratory of RNA Editing, Department of Pediatric Haematology Oncology, Bambino Gesu Children s Hospital, IRCCS, Piazza S, Onofrio 4, Rome 00165, Italy
    BMC Cancer 13:255. 2013
  7. doi request reprint Allogeneic hematopoietic stem cell transplantation in children with sickle cell disease
    Franco Locatelli
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, Roma, Italy
    Pediatr Blood Cancer 59:372-6. 2012
  8. doi request reprint Strategies to optimize the outcome of children given T-cell depleted HLA-haploidentical hematopoietic stem cell transplantation
    Franco Locatelli
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, Piazza S Onofrio, Rome, Italy
    Best Pract Res Clin Haematol 24:339-49. 2011
  9. pmc Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors
    Maria Antonietta Avanzini
    Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
    Haematologica 94:1649-60. 2009
  10. doi request reprint Ex vivo expansion of mesenchymal stromal cells
    Maria Ester Bernardo
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, P le Sant Onofrio, 4, 00165 Roma, Italy
    Best Pract Res Clin Haematol 24:73-81. 2011

Detail Information

Publications84

  1. ncbi request reprint Natural killer cells in the treatment of high-risk acute leukaemia
    Franco Locatelli
    Department of Paediatric Haematology and Oncology, IRCCS Bambino Gesù Children s Hospital, Rome, Italy University of Pavia, Pavia, Italy Electronic address
    Semin Immunol 26:173-9. 2014
    ....
  2. doi request reprint Current and future approaches to treat graft failure after allogeneic hematopoietic stem cell transplantation
    Franco Locatelli
    IRCCS Ospedale Pediatrico Bambino Gesù, Department of Pediatric Oncology and Hematology, Piazza Sant Onofrio 4, 00165 Rome, Italy 39 06 68592678 39 06 68592292
    Expert Opin Pharmacother 15:23-36. 2014
    ..Factors associated with graft failure include HLA disparity in the donor/recipient pair, underlying disease, viral infections, type of conditioning regimen and stem cell source employed...
  3. ncbi request reprint Negative depletion of α/β+ T cells and of CD19+ B lymphocytes: a novel frontier to optimize the effect of innate immunity in HLA-mismatched hematopoietic stem cell transplantation
    Franco Locatelli
    Department of Pediatric Hematology and Oncology, IRCCS Ospedale Bambino Gesù, Rome, Italy University of Pavia, Italy Electronic address
    Immunol Lett 155:21-3. 2013
    ..Preliminary results on 60 children transplanted so far after this type of graft manipulation are particularly promising. ..
  4. doi request reprint At the Bedside: Innate immunity as an immunotherapy tool for hematological malignancies
    Franco Locatelli
    1 Piazza Sant Onofrio 4, 00165 Rome, Italy
    J Leukoc Biol 94:1141-57. 2013
    ..Basic aspects of innate immunity relevant to the field will be covered by a companion review article. ..
  5. doi request reprint Outcome of patients with hemoglobinopathies given either cord blood or bone marrow transplantation from an HLA-identical sibling
    Franco Locatelli
    Department of Pediatric Hematology Oncology, Istituto di Ricovero e Cura a Carattere Scientifico, Ospedale Pediatrico Bambino Gesu, Piazzale Sant Onofrio 4, Rome, Italy
    Blood 122:1072-8. 2013
    ..In multivariate analysis, DFS did not differ between CBT and BMT recipients. Patients with TM or SCD have excellent outcomes after both HLA-identical sibling CBT and BMT. ..
  6. pmc ADAR2 editing activity in newly diagnosed versus relapsed pediatric high-grade astrocytomas
    Sara Tomaselli
    Laboratory of RNA Editing, Department of Pediatric Haematology Oncology, Bambino Gesu Children s Hospital, IRCCS, Piazza S, Onofrio 4, Rome 00165, Italy
    BMC Cancer 13:255. 2013
    ....
  7. doi request reprint Allogeneic hematopoietic stem cell transplantation in children with sickle cell disease
    Franco Locatelli
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, Roma, Italy
    Pediatr Blood Cancer 59:372-6. 2012
    ..Likewise, transplant strategies based on the use of reduced-intensity regimens and the induction of mixed chimerism have been explored to decrease allo-HSCT short- and long-term complications...
  8. doi request reprint Strategies to optimize the outcome of children given T-cell depleted HLA-haploidentical hematopoietic stem cell transplantation
    Franco Locatelli
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, Piazza S Onofrio, Rome, Italy
    Best Pract Res Clin Haematol 24:339-49. 2011
    ..Novel and effective strategies aimed at further augmenting the graft-versus-leukemia effect and at optimizing prevention/treatment of opportunistic/viral infections are warranted...
  9. pmc Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors
    Maria Antonietta Avanzini
    Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
    Haematologica 94:1649-60. 2009
    ..However, relatively little is known about the mechanisms responsible for their immunomodulatory effects, which could be influenced by both the cell source and culture conditions...
  10. doi request reprint Ex vivo expansion of mesenchymal stromal cells
    Maria Ester Bernardo
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, P le Sant Onofrio, 4, 00165 Roma, Italy
    Best Pract Res Clin Haematol 24:73-81. 2011
    ..Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed...
  11. doi request reprint Human cytomegalovirus-specific CD4(+) and CD8(+) T-cell response determination: comparison of short-term (24h) assays vs long-term (7-day) infected dendritic cell assay in the immunocompetent and the immunocompromised host
    Paola Zelini
    Service of Virology and Microbiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
    Clin Immunol 136:269-81. 2010
    ..A significant correlation between iDC-activated T-cells and T-cells activated by either iCL or the pep-pool was observed. In conclusion, whenever a rapid result is needed, short-term assays may efficiently replace the iDC assay...
  12. pmc Monitoring of human cytomegalovirus and virus-specific T-cell response in young patients receiving allogeneic hematopoietic stem cell transplantation
    Daniele Lilleri
    Laboratori Sperimentali di Ricerca, Area Trapiantologica, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
    PLoS ONE 7:e41648. 2012
    ..The decision to treat HCMV infection in young HSCT recipients may be taken by combining virological and immunological findings...
  13. doi request reprint Transplantation in the onco-hematology field: focus on the manipulation of αβ and γδ T cells
    Nicola Daniele
    Stem Cells Processing Laboratory, Pediatric Hospital Bambino Gesu, IRCCS, Rome, Italy
    Pathol Res Pract 208:67-73. 2012
    ..These "facilitating" cells might facilitate engraftment, exert GvL effects, and reduce the risk for infections...
  14. ncbi request reprint Treatment of EBV-related post-renal transplant lymphoproliferative disease with a tailored regimen including EBV-specific T cells
    Patrizia Comoli
    Laboratory of Transplant Immunology and Pediatric Hematology Oncology, IRCCS Policlinico S Matteo, Pavia, Italy
    Am J Transplant 5:1415-22. 2005
    ..These preliminary results suggest that a combined chemoimmunotherapy regimen including virus-specific T-cells is well tolerated and potentially effective as first-line treatment of EBV-related PTLD...
  15. pmc T and NK cells: two sides of tumor immunoevasion
    Doriana Fruci
    Paediatric Haematology Oncology Department, Bambino Gesu Children s Hospital, IRCCS, Piazza Sant Onofrio 4, Rome 00165, Italy
    J Transl Med 11:30. 2013
    ..Shared (T/NK cells) natural immunosuppressants and activating/inhibitory ligands expressed by tumor cells may provide mechanistic insight into impaired gathering and function of immune effectors at the tumor site...
  16. ncbi request reprint The outcome of children with Fanconi anemia given hematopoietic stem cell transplantation and the influence of fludarabine in the conditioning regimen: a report from the Italian pediatric group
    Franco Locatelli
    Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo e Università di Pavia, Pavia, Italy
    Haematologica 92:1381-8. 2007
    ..While in the past HSCT from an HLA-identical sibling was proven to cure many patients, a higher incidence of treatment failure has been reported in recipients of an unrelated donor (UD) or HLA-partially matched related allograft...
  17. doi request reprint Human cytomegalovirus-specific T cell reconstitution in young patients receiving T cell-depleted, allogeneic hematopoietic stem cell transplantation
    Daniele Lilleri
    Servizio di Virologia, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Universita di Pavia, Pavia, Italy
    J Infect Dis 199:829-36. 2009
    ..Scarce data on this subject are available for children receiving a T cell-depleted HSCT...
  18. doi request reprint Cord blood transplantation in children with haematological malignancies
    Alice Bertaina
    Department of Paediatric Haematology Oncology, IRCCS Ospedale Bambino Gesù, Rome, Italy
    Best Pract Res Clin Haematol 23:189-96. 2010
    ....
  19. doi request reprint Allogeneic hematopoietic stem cell transplantation in thalassemia major: results of a reduced-toxicity conditioning regimen based on the use of treosulfan
    Maria Ester Bernardo
    Department of Pediatric Hematology Oncology, Istituto di Ricovero e Cura a Carattere Scientifico, Bambino Gesu Children s Hospital, Piazzale S Onofrio, Rome, Italy
    Blood 120:473-6. 2012
    ..Neither the class of risk nor the donor used influenced outcome. This treosulfan-based preparation proved to be safe and effective for thalassemia patients given allogeneic HSCT...
  20. doi request reprint Phenotypical/functional characterization of in vitro-expanded mesenchymal stromal cells from patients with Crohn's disease
    Maria Ester Bernardo
    Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
    Cytotherapy 11:825-36. 2009
    ..The aim of the study was in vitro characterization of MSC from active CD patients for future clinical application...
  21. ncbi request reprint Use of a DNAemia cut-off for monitoring human cytomegalovirus infection reduces the number of preemptively treated children and young adults receiving hematopoietic stem-cell transplantation compared with qualitative pp65 antigenemia
    Daniele Lilleri
    Servizio di Virologia, Universita di Pavia, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico IRCCS Policlinico San Matteo, Pavia, Italy
    Blood 110:2757-60. 2007
    ..259), the number of patients treated was significantly lower in the DNAemia arm (18% vs 31%, P = .026). No patient developed HCMV disease. The use of a DNAemia cut-off avoids unnecessary antiviral treatment...
  22. doi request reprint Allogeneic hematopoietic stem cell transplantation may restore gluten tolerance in patients with celiac disease
    Rachele Ciccocioppo
    Clinica Medica I, Fondazione IRCCS Policlinico San Matteo, Universita degli Studi di Pavia, Piazzale Golgi 19, Pavia, Italy
    J Pediatr Gastroenterol Nutr 56:422-7. 2013
    ..These data suggest that allogeneic HSCT may lead to induction of gluten tolerance in patients with CD...
  23. ncbi request reprint Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation
    Matteo Di Nardo
    Department of Pediatric Intensive Care, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
    Pediatr Blood Cancer 61:376-9. 2014
    ..T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration...
  24. ncbi request reprint Variations of the perforin gene in patients with autoimmunity/lymphoproliferation and defective Fas function
    Rita Clementi
    Pediatric Haematology Oncology, Istituto di Ricovero e Cura a Carattere Scientifico IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy
    Blood 108:3079-84. 2006
    ..A91V patients displayed lower NK activity than controls. These data suggest that perforin variations are a susceptibility factor for ALPS/DALD development in subjects with defective Fas function and may influence disease expression...
  25. ncbi request reprint Hematopoietic stem cell transplantation (HSCT) in children with juvenile myelomonocytic leukemia (JMML): results of the EWOG-MDS/EBMT trial
    Franco Locatelli
    Oncoematologia Pediatrica, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
    Blood 105:410-9. 2005
    ..Disease recurrence remains the major cause of treatment failure. Outcome of UD-HSCT recipients is comparable to that of children receiving transplants from an HLA-identical sibling...
  26. doi request reprint Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease
    Rachele Ciccocioppo
    Clinica Medica I, Fondazione IRCCS Policlinico San Matteo, Universita degli Studi di Pavia, Pavia, Italy
    Gut 60:788-98. 2011
    ..We therefore investigated the feasibility, safety and efficacy of serial intrafistular injections of autologous bone marrow-derived mesenchymal stromal cells (MSCs) in the treatment of fistulising Crohn's disease...
  27. doi request reprint Processing of hematopoietic stem cells from peripheral blood before cryopreservation: use of a closed automated system
    Francesco Zinno
    Immunohematology Section, Tor Vergata University, and SIMT, Pediatric Hematology and Oncology, IRCCS Bambino Gesù Pediatric Hospital, Rome, Italy
    Transfusion 51:2656-63. 2011
    ..Cell processing that precedes cryopreservation of HPC-A includes volume reduction aimed at reducing the amount of dimethyl sulfoxide used, as well as storage space...
  28. ncbi request reprint Two novel cases of trilateral retinoblastoma: genetics and review of the literature
    Gemma D'Elia
    Cytogenetics and Molecular Genetics Unit, Bambino Gesu Children s Hospital, Rome, Italy
    Cancer Genet 206:398-401. 2013
    ..Moreover, our study highlights the need for new approaches allowing a multigenic analysis to clarify the genotype-phenotype correlation in TRB. ..
  29. doi request reprint HLA-haploidentical T cell-depleted allogeneic hematopoietic stem cell transplantation in children with Fanconi anemia
    Marco Zecca
    Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
    Biol Blood Marrow Transplant 20:571-6. 2014
    ..To the best of our knowledge, this is the largest series of hematopoietic stem cell transplantation from a haploidentical related donor in FA patients reported to date...
  30. pmc Intestinal dendritic cells in the pathogenesis of inflammatory bowel disease
    Sergio Rutella
    Department of Pediatric Hematology Oncology, IRCCS Children s Hospital Bambino Gesù 00165 Rome, Italy
    World J Gastroenterol 17:3761-75. 2011
    ..It also addresses the potential clinical benefit derived from DC targeting either in vivo or in vitro...
  31. pmc IRF1 and NF-kB restore MHC class I-restricted tumor antigen processing and presentation to cytotoxic T cells in aggressive neuroblastoma
    Silvia Lorenzi
    Paediatric Haematology Oncology Department, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy
    PLoS ONE 7:e46928. 2012
    ..These findings provide molecular insight into defective MHC-I expression in NB cells and provide the rational for T cell-based immunotherapy in NB variants refractory to conventional therapy...
  32. ncbi request reprint Low percentages of circulating CD8(+)/CD45RA(+) human T lymphocytes expressing beta7 integrin correlate with the occurrence of intestinal acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
    M Antonietta Avanzini
    Pediatric Hematology Oncology and Laboratory of Transplant Immunology, IRCCS Policlinico San Matteo Pavia, University of Pavia, Pavia, Italy
    Exp Hematol 34:1429-34. 2006
    ..Therefore, we evaluated the correlation existing between circulating beta7(+) T-lymphocyte subsets early after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and occurrence of a-GVHD...
  33. doi request reprint The strange case of the lost NRAS mutation in a child with juvenile myelomonocytic leukemia
    Paola De Filippi
    Genetica Medica, Universita degli Studi di Pavia, Pavia, Italy
    Pediatr Blood Cancer 59:580-2. 2012
    ..This case also indicates that molecular analysis on cell types other than peripheral blood leukocytes may be useful to obtain relevant biological information on JMML pathogenesis...
  34. ncbi request reprint T-cell lines specific for peptides of adenovirus hexon protein and devoid of alloreactivity against recipient cells can be obtained from HLA-haploidentical donors
    Patrizia Comoli
    Pediatric Hematology Oncology, Fondazione IRCCS Policlinico S Matteo, University of Pavia, Pavia, Italy
    J Immunother 31:529-36. 2008
    ..These T cells may be safely employed for adoptive treatment of HAdV complications...
  35. ncbi request reprint Haploidentical hemopoietic stem cell transplantation for the treatment of high-risk leukemias: how NK cells make the difference
    Franco Locatelli
    Pediatric Hematology Oncology, University of Pavia, IRCCS Foundation, Policlinico San Matteo, Pavia, Italy
    Clin Immunol 133:171-8. 2009
    ..In view of the favorable clinical outcome of children with chemo-resistant ALL, Haplo HSCT from an NK-alloreactive relative could become a first option in these high-risk leukemia patients...
  36. pmc Hyper-activation of Notch3 amplifies the proliferative potential of rhabdomyosarcoma cells
    Maria De Salvo
    Department of Oncohematology, Ospedale Pediatrico Bambino Gesu, IRCCS, Roma, Italy
    PLoS ONE 9:e96238. 2014
    ..These results strongly suggest that high levels of Notch3 activation increase the proliferative potential of RMS cells. ..
  37. doi request reprint Unmanipulated donor lymphocytes for EBV-related PTLD after T-cell depleted HLA-haploidentical transplantation
    Maria Debora De Pasquale
    Department of Pediatric Hematology Oncology, Ospedale Bambino Gesu, Rome, Italy
    Pediatrics 129:e189-94. 2012
    ..This case demonstrates that repeated infusions of controlled numbers of donor CD3+ cells cure EBV-related PTLD in haplo-HSCT without inducing graft-versus-host disease...
  38. pmc Analysis of risk factors influencing outcomes after cord blood transplantation in children with juvenile myelomonocytic leukemia: a EUROCORD, EBMT, EWOG-MDS, CIBMTR study
    Franco Locatelli
    Department of Pediatric Hematology and Oncology, Istituto di Ricovero e Cura a Carattere Scientifico IRCCS Ospedale Bambino Gesù, Universita di Pavia, Rome, Italy
    Blood 122:2135-41. 2013
    ..Because disease recurrence remains the major cause of treatment failure, strategies to reduce incidence of relapse are warranted. ..
  39. doi request reprint T(reg) cells: collection, processing, storage and clinical use
    Nicola Daniele
    Immunohematology Section, Tor Vergata University and SIMT, IRCCS Bambino Gesù Pediatric Hospital, Rome, Italy
    Pathol Res Pract 207:209-15. 2011
    ..Efficient isolation, expansion and cryopreservation strategies that comply with Good Manufacturing Practice (GMP) guidelines are prerequisites for the clinical application of human CD4+ CD25+ CD127(low) FOXP3+ regulatory T cells...
  40. doi request reprint The role of reduced intensity preparative regimens in patients with thalassemia given hematopoietic transplantation
    Alice Bertaina
    Department of Paediatric Haematology Oncology, University of Pavia, Ospedale Bambino Gesu, Rome, Italy
    Ann N Y Acad Sci 1202:141-8. 2010
    ..This novel approach is a promising alternative for reducing the risk of life-threatening complications and increasing the number of TM patients successfully cured with an allograft...
  41. pmc Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia
    Antonella Conforti
    Department of Pediatric Hematology Oncology, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
    PLoS ONE 8:e76989. 2013
    ..Our findings indicate that ALL-MSCs display reduced ability to proliferate and to support long-term hematopoiesis in vitro. ALL-MSCs isolated at diagnosis do not differ from those obtained during treatment. ..
  42. ncbi request reprint Management of relapsed acute lymphoblastic leukemia in childhood with conventional and innovative approaches
    Franco Locatelli
    aDepartment of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children s Hospital, Rome bUniversity of Pavia, Pavia, Italy
    Curr Opin Oncol 25:707-15. 2013
    ....
  43. doi request reprint Cord blood transplantation in patients with hemoglobinopathies
    Agata Boncimino
    Pediatric Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy
    Transfus Apher Sci 42:277-81. 2010
    ..Available evidence indicates that related donor CBT is a safe and effective option for patients with hemoglobinopathies, able to offer results at least as good as those reported using bone marrow cells...
  44. doi request reprint Extracorporeal photopheresis for the treatment of acute and chronic graft-versus-host disease in adults and children: best practice recommendations from an Italian Society of Hemapheresis and Cell Manipulation (SIdEM) and Italian Group for Bone Marrow Tra
    Luca Pierelli
    Servizio di Immunoematologia e Medicina Trasfusionale, A O San Camillo Forlanini, Roma, Italy Università La Sapienza, Roma, Italy Servizio Trasfusionale, UOS Aferesi e nuove tecnologie trasfusionali, A O San Gerardo, Monza, Italy Unità di Ematologia, Divisione di Medicina Interna, Ospedale C Massaia, Asti, Italy Clinica di Oncoematologia Pediatrica, Universita di Padova, Padova, Italy Servizio di Immunoematologia e Trasfusionale, Unità di Terapia Cellulare, Fondazione IRCCS Policlinico S Matteo, Pavia, Italy UO Immunoematologia, A O Universitaria Pisana, Pisa, Italy Divisione Ematologia e Trapianto di Midollo Osseo, Ospedale San Martino, Genova, Italy Dipartimento of Oncologia Ematologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, Pavia, Italy Università di Pavia, Pavia, Italy Centro Trasfusionale, Ospedale San Martino, Genova, Italy UO Ematologia, A O Careggi and Università di Firenze, Firenze, Italy
    Transfusion 53:2340-52. 2013
    ..Consensus-based recommendations ameliorate the appropriateness in daily clinical practice and, in turn, optimize the use of health care resources...
  45. ncbi request reprint Lymphocyte proliferation specific for recall, CMV and HIV antigens in miniaturized and automated format
    Giuseppina Li Pira
    Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children Hospital, Rome, Italy
    J Immunol Methods 384:135-42. 2012
    ..This procedure is consistent with our policy to miniaturize assays for specific T-cell immunity, as we have already established for cytokine secretion assays...
  46. doi request reprint How I treat relapsed childhood acute lymphoblastic leukemia
    Franco Locatelli
    Department of Pediatric Hematology Oncology, Istituto di Ricovero e Cura a Carattere Scientifico Bambino Gesù Children s Hospital, Rome, Italy
    Blood 120:2807-16. 2012
    ..Innovative approaches, including new agents or strategies of immunotherapy, are under investigation in trials enrolling patients with resistant or more advanced disease...
  47. pmc Ewing sarcoma of the bone in children under 6 years of age
    Maria Antonietta De Ioris
    Haematology Oncology Department, Ospedale Pediatrico Bambino Gesù IRCCS, Rome, Italy
    PLoS ONE 8:e53223. 2013
    ..Ewing Sarcoma Family Tumours (ESFT) are rare in early childhood. The aim of this study was to report the clinical characteristics and outcome of children under 6 years of age affected by ESFT of the bone in Italy...
  48. pmc Miniaturized and high-throughput assays for analysis of T-cell immunity specific for opportunistic pathogens and HIV
    Giuseppina Li Pira
    Bambino Gesu Children s Hospital, Rome, Italy
    Clin Vaccine Immunol 21:488-95. 2014
    ....
  49. ncbi request reprint Human bone marrow derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms
    Maria Ester Bernardo
    Oncoematologia Pediatrica, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Universita di Pavia, Pavia, Italy
    Cancer Res 67:9142-9. 2007
    ..ALT was not evidenced in the MSCs tested. BM-derived MSCs can be safely expanded in vitro and are not susceptible to malignant transformation, thus rendering these cells suitable for cell therapy approaches...
  50. pmc MicroRNAs in rhabdomyosarcoma: pathogenetic implications and translational potentiality
    Rossella Rota
    Department of Oncohematology, Ospedale Pediatrico Bambino Gesu, IRCCS, Roma, Italy
    Mol Cancer 10:120. 2011
    ..Additionally, we examine the potential of microRNAs as prognostic and diagnostic markers in this soft-tissue sarcoma, and discuss possible therapeutic applications and challenges of a "microRNA therapy"...
  51. pmc Indoleamine 2,3-dioxygenase 1 (IDO1) activity in leukemia blasts correlates with poor outcome in childhood acute myeloid leukemia
    Valentina Folgiero
    Department of Pediatric Hematology Oncology and Transfusion Medicine, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
    Oncotarget 5:2052-64. 2014
    ..Moreover, they speak in favor of the hypothesis that IDO can be targeted, in adjunct to current chemotherapy approaches, to improve the clinical outcome of children with AML. ..
  52. pmc Notch signaling in pediatric soft tissue sarcomas
    Rossella Rota
    Department of Oncohematology, Ospedale Pediatrico Bambino Gesu, IRCCS, Piazza Sant Onofrio 4, Roma, 00165, Italy
    BMC Med 10:141. 2012
    ..The potential challenges and opportunities of targeting Notch signaling in the treatment of pediatric soft tissue sarcomas are also discussed...
  53. ncbi request reprint Human cytomegalovirus immediate-early mRNAemia versus pp65 antigenemia for guiding pre-emptive therapy in children and young adults undergoing hematopoietic stem cell transplantation: a prospective, randomized, open-label trial
    Giuseppe Gerna
    Servizio di Virologia, Division di Oncoematologia Pediatrica, Istituto di Ricovero e Cura a Carattee Scientifico Policlinico San Matteo, Pavia, Italy
    Blood 101:5053-60. 2003
    ..Although these data indicate that IEmRNA determination does not offer advantages in terms of treatment duration, it can safely replace antigenemia, while semiautomation is the major advantage of the NASBA procedure...
  54. pmc Cellular and molecular basis of haploidentical hematopoietic stem cell transplantation in the successful treatment of high-risk leukemias: role of alloreactive NK cells
    Franco Locatelli
    Department of Pediatric Hematology Oncology, Istituto di Ricovero e Cura a Carattere Scientifico, Ospedale Pediatrico Bambino Gesù Rome, Italy Università di Pavia Pavia, Italy
    Front Immunol 4:15. 2013
    ..Novel approaches are in progress to further improve the clinical outcome based on the infusion of donor alloreactive NK cells either as a component of the transplanted cell population or as in vitro expanded NK cells...
  55. ncbi request reprint B lymphocyte reconstitution after hematopoietic stem cell transplantation: functional immaturity and slow recovery of memory CD27+ B cells
    Maria Antonietta Avanzini
    Laboratorio di Immunologia dei Trapianti e Oncoematologia Pediatrica, IRCCS Policlinico San Matteo, Pavia, University of Pavia, Pavia, Italy
    Exp Hematol 33:480-6. 2005
    ..HSCT recipients may obtain protective titers of pathogen-specific antibody through vaccination, but optimal timing of reimmunization remains to be defined...
  56. doi request reprint A-to-I RNA editing: the "ADAR" side of human cancer
    Federica Galeano
    RNA editing Laboratory, Oncohaematology Department, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, Italy
    Semin Cell Dev Biol 23:244-50. 2012
    ..This review summarizes the available knowledge on A-to-I RNA editing in the cancer field, giving a new view on how ADARs may play a role in carcinogenesis...
  57. pmc Multicluster nosocomial outbreak of parainfluenza virus type 3 infection in a pediatric oncohematology unit: a phylogenetic study
    Antonio Piralla
    Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
    Haematologica 94:833-9. 2009
    ..Human parainfluenza virus type 3 (hPIV-3) has been reported to cause nosocomial outbreaks of respiratory infection, in particular among hematopoietic stem cell transplantation recipients...
  58. doi request reprint Natural killer cells efficiently reject lymphoma silenced for the endoplasmic reticulum aminopeptidase associated with antigen processing
    Loredana Cifaldi
    Oncohaematology Department, IRCCS, Ospedale Pediatrico Bambino Gesu, Rome, Italy
    Cancer Res 71:1597-606. 2011
    ..Because a large fraction of human tumors express high levels of the homologous ERAP1 and/or ERAP2, the present findings highlight a convenient, novel target for cancer immunotherapy...
  59. ncbi request reprint Extracorporeal photopheresis for paediatric patients experiencing graft-versus-host disease (GVHD)
    Sergio Rutella
    Department of Paediatric Haematology Oncology and Transfusion Medicine, IRCCS Bambino Gesù Children s Hospital, Rome, Italy Electronic address
    Transfus Apher Sci 50:340-8. 2014
    ..Herein, we will focus on current clinical trials with ECP in children with GVHD, with an emphasis on technical and clinical issues that are peculiar to the paediatric setting. ..
  60. doi request reprint Preserved antibody levels and loss of memory B cells against pneumococcus and tetanus after splenectomy: tailoring better vaccination strategies
    M Manuela Rosado
    Immunology Area, Bambino Gesu Children s Hospital IRCCS, Rome, Italy
    Eur J Immunol 43:2659-70. 2013
    ....
  61. ncbi request reprint Chronic graft-versus-host disease in children: incidence, risk factors, and impact on outcome
    Marco Zecca
    Oncoematologia Pediatrica, Universita di Pavia, IRCCS Policlinico San Matteo, Pavia, Italy
    Blood 100:1192-200. 2002
    ..Use of CB stem cells and preparative regimens without radiotherapy may prevent its development. In patients affected by ALL, cGVHD was associated with a strong graft-versus-leukemia effect, improving DFS...
  62. ncbi request reprint New insights into haematopoietic stem cell transplantation for patients with haemoglobinopathies
    Franco Locatelli
    Oncoematologia Pediatrica, IRCCS Policlinico S Matteo, Pavia, Italy
    Br J Haematol 125:3-11. 2004
  63. doi request reprint Expression of multidrug resistance-associated proteins in paediatric soft tissue sarcomas before and after chemotherapy
    Arianna Citti
    Pathology Department, IRCCS, Ospedale Pediatrico Bambino Gesu, I 00165 Rome, Italy
    Int J Oncol 41:117-24. 2012
    ....
  64. pmc Oligophrenin-1 (OPHN1), a gene involved in X-linked intellectual disability, undergoes RNA editing and alternative splicing during human brain development
    Sabina Barresi
    Molecular Medicine Laboratory, Neurosciences Department, Bambino Gesu Children s Hospital IRCCS, Rome, Italy
    PLoS ONE 9:e91351. 2014
    ..Our results demonstrate that multiple post-transcriptional events occur on OPHN1, a gene playing an important role in brain function and development. ..
  65. pmc High-resolution array CGH profiling identifies Na/K transporting ATPase interacting 2 (NKAIN2) as a predisposing candidate gene in neuroblastoma
    Paolo Romania
    Paediatric Haematology Oncology Department, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy
    PLoS ONE 8:e78481. 2013
    ..Taken together, these data indicate a potential role of NKAIN2 gene in NB growth and differentiation. ..
  66. doi request reprint Prophylaxis and treatment of invasive fungal diseases in allogeneic stem cell transplantation: results of a consensus process by Gruppo Italiano Trapianto di Midollo Osseo (GITMO)
    Corrado Girmenia
    Department of Hematology, Policlinico Umberto I, University of Sapienza, Rome, Italy
    Clin Infect Dis 49:1226-36. 2009
    ....
  67. ncbi request reprint Familial platelet disorder with propensity to acute myelogenous leukemia: genetic heterogeneity and progression to leukemia via acquisition of clonal chromosome anomalies
    Antonella Minelli
    Biologia Generale e Genetica Medica, Universita di Pavia, Pavia, Italy
    Genes Chromosomes Cancer 40:165-71. 2004
    ....
  68. pmc The cannabinoid receptor type 2 as mediator of mesenchymal stromal cell immunosuppressive properties
    Francesca Rossi
    Department of Women, Child and General and Specialistic Surgery, Second University of Naples, Naples, Italy Department of Onco Haematology, IRCCS Bambino Gesù Children Hospital, Rome, Italy
    PLoS ONE 8:e80022. 2013
    ....
  69. ncbi request reprint The Yin and Yang of non-neuronal α7-nicotinic receptors in inflammation and autoimmunity
    Perla Filippini
    System Approaches and Non Communicable Disease Lab, IRCCS San Raffaele Pisana, Rome, Italy
    Curr Drug Targets 13:644-55. 2012
    ..These data may portend favorable implications for the targeted treatment of chronic and debilitating human disorders, such as diabetes, arthritis, asthma and inflammatory bowel disease, with α7-selective ligands...
  70. ncbi request reprint Inherited perforin and Fas mutations in a patient with autoimmune lymphoproliferative syndrome and lymphoma
    Rita Clementi
    Department of Biology and Medical Genetics, University of Pavia, Pavia, Italy
    N Engl J Med 351:1419-24. 2004
    ..The combined effect of the two mutant genes may have contributed to the development of the autoimmune lymphoproliferative syndrome and lymphoma in this patient...
  71. ncbi request reprint Dendritic cells pulsed with polyomavirus BK antigen induce ex vivo polyoma BK virus-specific cytotoxic T-cell lines in seropositive healthy individuals and renal transplant recipients
    Patrizia Comoli
    Laboratory of Transplant Immunology and Pediatric Hematology Oncology, IRCCS Policlinico S Matteo, Pavia, Italy
    J Am Soc Nephrol 14:3197-204. 2003
    ..Application of this culture system may allow a preemptive therapy approach to BKV-related complications in transplant recipients, based on CTL treatment guided by BKV DNA levels...
  72. doi request reprint Germ-line mutation of the NRAS gene may be responsible for the development of juvenile myelomonocytic leukaemia
    Paola De Filippi
    Medical Genetics, Department of Human and Hereditary Pathology, University of Pavia, Pavia, Italy
    Br J Haematol 147:706-9. 2009
    ..This case suggests for the first time that constitutional mutations of NRAS may be responsible for development of a myeloproliferative/myelodysplastic disorder in children...
  73. doi request reprint Gene expression-based classification as an independent predictor of clinical outcome in juvenile myelomonocytic leukemia
    Silvia Bresolin
    Laboratory of Oncohematology, Department of Pediatrics, University of Padova, Pavia, Italy
    J Clin Oncol 28:1919-27. 2010
    ..Gene expression-based classification could thus be prospectively used to guide clinical/therapeutic decisions...
  74. doi request reprint Mesenchymal stromal cells
    Maria Ester Bernardo
    Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
    Ann N Y Acad Sci 1176:101-17. 2009
    ....
  75. pmc Pharmacological inhibition of EZH2 as a promising differentiation therapy in embryonal RMS
    Roberta Ciarapica
    Department of Oncohematology, Laboratory of angiogenesis, Ospedale Pediatrico Bambino Gesu, IRCCS, Piazza S, Onofrio 4, 00165 Rome, Italy
    BMC Cancer 14:139. 2014
    ....
  76. ncbi request reprint Ethical reappraisal of 15 years of cord-blood transplantation
    Giuseppe Roberto Burgio
    Department of Paediatrics, University of Pavia, Istituto di Ricovero e Cura a Carattere Scientifico, Policlinico San Matteo, Pavia, Italy
    Lancet 361:250-2. 2003
    ....
  77. pmc Targeting multiple-myeloma-induced immune dysfunction to improve immunotherapy outcomes
    Sergio Rutella
    Department of Pediatric Hematology Oncology, IRCCS Bambino Gesù Children s Hospital, Piazza Sant Onofrio 4, 00165 Rome, Italy
    Clin Dev Immunol 2012:196063. 2012
    ..This article summarizes our current knowledge on the molecular determinants of immune evasion in patients with MM and highlights how these pathways can be targeted to improve patients' clinical outcome...
  78. doi request reprint Cilostazol promotes angiogenesis after peripheral ischemia through a VEGF-dependent mechanism
    Federico Biscetti
    Laboratory of Vascular Biology and Genetics, Department of Medicine, A Gemelli University Hospital, Catholic University School of Medicine, Rome, Italy
    Int J Cardiol 167:910-6. 2013
    ..The aim of this work was to investigate the effects of cilostazol on angiogenic response in a murine model of peripheral ischemia and to clarify the underlying molecular mechanisms of that response...
  79. pmc Indoleamine 2,3-dioxygenase 1 (IDO1) activity correlates with immune system abnormalities in multiple myeloma
    Giuseppina Bonanno
    Department of Gynecology, Catholic University Med School, Rome, Italy
    J Transl Med 10:247. 2012
    ..It is presently unknown whether MM cells express IDO1 and whether IDO1 activity correlates with immune system impairment...
  80. doi request reprint The putative role of endoplasmic reticulum aminopeptidases in autoimmunity: insights from genomic-wide association studies
    Alessandra Fierabracci
    Immunology Area, Bambino Gesu Children s Hospital IRCCS, Rome, Italy
    Autoimmun Rev 12:281-8. 2012
    ....
  81. doi request reprint ADARs: allies or enemies? The importance of A-to-I RNA editing in human disease: from cancer to HIV-1
    Angela Gallo
    RNA editing Laboratory, Oncohaematology Department, IRCCS, Ospedale Pediatrico Bambino Gesu, Rome, Italy
    Biol Rev Camb Philos Soc 87:95-110. 2012
    ..This review summarises available recent knowledge on A-to-I RNA editing and ADAR enzymes, with particular attention given to the emerging role played by these enzymes in cancer, some infectious diseases and immune-mediated disorders...
  82. pmc Transplantation and innate immunity: the lesson of natural killer cells
    Alice Bertaina
    Paediatric Haematology Oncology, University of Pavia, Foundation IRCCS Policlinico San Matteo, Pavia, Italy
    Ital J Pediatr 35:44. 2009
    ..This study represents a major breakthrough in the cure of otherwise fatal leukemias, providing information on the best criteria for choosing the optimal donor...
  83. ncbi request reprint Incidence and pathogenesis of tumor lysis syndrome
    Franco Locatelli
    Oncoematologia Pediatrica, IRCCS Policlinico San Matteo, Pavia, Italy
    Contrib Nephrol 147:61-8. 2005
    ..A good comprehension of TLS pathophysiology has provided the basis for an effective and rational treatment of this complication, adversely affecting the outcome of cancer patients...
  84. pmc Enhancer of zeste homolog 2 (EZH2) in pediatric soft tissue sarcomas: first implications
    Roberta Ciarapica
    Department of Oncohematology, IRCCS, Ospedale Pediatrico Bambino Gesu, Roma, Italy
    BMC Med 9:63. 2011
    ..Finally, the therapeutic potential of epigenetic therapies modulating EZH2-mediated gene repression is considered...