Alessandro Vitale

Summary

Affiliation: Istituto di Biologia e Biotecnologia Agraria
Country: Italy

Publications

  1. ncbi request reprint Recombinant pharmaceuticals from plants: the plant endomembrane system as bioreactor
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy
    Mol Interv 5:216-25. 2005
  2. ncbi request reprint Sorting of proteins to storage vacuoles: how many mechanisms?
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, CNR, 20133 Milano, Italy
    Trends Plant Sci 10:316-23. 2005
  3. doi request reprint An engineered C-terminal disulfide bond can partially replace the phaseolin vacuolar sorting signal
    Andrea Pompa
    Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Via della Madonna Alta 130, 06128 Perugia, Italy
    Plant J 61:782-91. 2010
  4. pmc Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study
    Carla Marusic
    Dipartimento BAS, Sezione Genetica e Genomica Vegetale, ENEA, C R Casaccia, Via Anguillarese 301, 00123, Rome, Italy
    Transgenic Res 18:499-512. 2009
  5. doi request reprint Endoplasmic reticulum quality control and the unfolded protein response: insights from plants
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
    Traffic 9:1581-8. 2008
  6. pmc Protein quality control mechanisms and protein storage in the endoplasmic reticulum. A conflict of interests?
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy
    Plant Physiol 136:3420-6. 2004
  7. doi request reprint Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuole
    Ombretta Foresti
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
    Mol Plant 1:1067-76. 2008
  8. pmc The human immunodeficiency virus antigen Nef forms protein bodies in leaves of transgenic tobacco when fused to zeolin
    Maddalena de Virgilio
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
    J Exp Bot 59:2815-29. 2008
  9. pmc Assembly and sorting of the tonoplast potassium channel AtTPK1 and its turnover by internalization into the vacuole
    Marie Maîtrejean
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy
    Plant Physiol 156:1783-96. 2011
  10. doi request reprint Anchorage to the cytosolic face of the endoplasmic reticulum membrane: a new strategy to stabilize a cytosolic recombinant antigen in plants
    Alessandra Barbante
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy
    Plant Biotechnol J 6:560-75. 2008

Collaborators

  • Sergio Arcioni
  • Maddalena de Virgilio
  • Katrin Czempinski
  • Lorenzo Frigerio
  • Doris Rentsch
  • Ingo Dreyer
  • Joachim Scholz-Starke
  • Bernd Mueller-Roeber
  • Bhakti Prinsi
  • Kathryn S Lilley
  • Julian K C Ma
  • M Bellucci
  • Emanuela Pedrazzini
  • Andrea Pompa
  • Marie Maîtrejean
  • Ombretta Foresti
  • Francesca De Marchis
  • Eva M Klein
  • Alessandra Rocchetti
  • James Nuttall
  • Carla Marusic
  • Alessandra Barbante
  • Gaetano Distefano
  • Silvana Castelli
  • Misoon Park
  • Manuela Cervelli
  • Davide Mainieri
  • Stefano Vavassori
  • Jane L Hadlington
  • Nataliya Y Komarova
  • Camilla Wulfetange
  • Alexandra Grippa
  • Tripti Sharma
  • Armando Carpaneto
  • Michael M Wudick
  • Camilla Voelker
  • Philip J Dix
  • Ralph Bock
  • Marcello Donini
  • Matthew S McCabe
  • Eugenio Benvenuto
  • Sarah Irons
  • Alessandra Gentile
  • Chris Hawes
  • Matteo Lorito
  • Ziniu Deng
  • Stefano La Malfa
  • Laura Ragni
  • Laura Mascheroni
  • Thilo Weimar
  • Paul Dupree
  • Inhwan Hwang
  • Riccardo Angelini
  • Paolo Mariottini
  • Rodolfo Federico
  • Alessandra Di Penta
  • Marika Rossi
  • Oriana Di Caro
  • Marco Archinti
  • Soo Jin Kim
  • Heidi Holkeri
  • Jurgen Denecke
  • Aniello Santoro

Detail Information

Publications24

  1. ncbi request reprint Recombinant pharmaceuticals from plants: the plant endomembrane system as bioreactor
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy
    Mol Interv 5:216-25. 2005
    ....
  2. ncbi request reprint Sorting of proteins to storage vacuoles: how many mechanisms?
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, CNR, 20133 Milano, Italy
    Trends Plant Sci 10:316-23. 2005
    ..A new array of possible molecular interactions is thus emerging that no longer supports a clear-cut distinction between the two types of vacuoles based on sorting signals and putative receptors...
  3. doi request reprint An engineered C-terminal disulfide bond can partially replace the phaseolin vacuolar sorting signal
    Andrea Pompa
    Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Via della Madonna Alta 130, 06128 Perugia, Italy
    Plant J 61:782-91. 2010
    ..Our experiments strongly support the idea that vacuolar delivery of phaseolin is promoted very early in the sorting process, when polypeptides are still contained within the ER, by homotypic interactions...
  4. pmc Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study
    Carla Marusic
    Dipartimento BAS, Sezione Genetica e Genomica Vegetale, ENEA, C R Casaccia, Via Anguillarese 301, 00123, Rome, Italy
    Transgenic Res 18:499-512. 2009
    ....
  5. doi request reprint Endoplasmic reticulum quality control and the unfolded protein response: insights from plants
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
    Traffic 9:1581-8. 2008
    ....
  6. pmc Protein quality control mechanisms and protein storage in the endoplasmic reticulum. A conflict of interests?
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy
    Plant Physiol 136:3420-6. 2004
  7. doi request reprint Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuole
    Ombretta Foresti
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
    Mol Plant 1:1067-76. 2008
    ..This strongly suggests the existence of a plant vacuolar sorting mechanism devoted to the disposal of defective secretory proteins...
  8. pmc The human immunodeficiency virus antigen Nef forms protein bodies in leaves of transgenic tobacco when fused to zeolin
    Maddalena de Virgilio
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
    J Exp Bot 59:2815-29. 2008
    ..These results provide insights into the requirements for PB formation and avoidance of quality-control degradation, and indicate a strategy for enhancing foreign protein accumulation in plants...
  9. pmc Assembly and sorting of the tonoplast potassium channel AtTPK1 and its turnover by internalization into the vacuole
    Marie Maîtrejean
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy
    Plant Physiol 156:1783-96. 2011
    ..Turnover of the fusion protein involves internalization into the vacuole where the GFP domain is released. This indicates a possible mechanism for the turnover of tonoplast proteins...
  10. doi request reprint Anchorage to the cytosolic face of the endoplasmic reticulum membrane: a new strategy to stabilize a cytosolic recombinant antigen in plants
    Alessandra Barbante
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy
    Plant Biotechnol J 6:560-75. 2008
    ..These results open up the way to use tail-anchors to improve foreign protein stability in the plant cytosol without perturbing cellular functions...
  11. pmc Zeolin. A new recombinant storage protein constructed using maize gamma-zein and bean phaseolin
    Davide Mainieri
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milano, Italy
    Plant Physiol 136:3447-56. 2004
    ..Because the storage proteins of cereals and legumes nutritionally complement each other, zeolin can be used as a starting point to produce nutritionally balanced and highly stable chimeric storage proteins...
  12. pmc How are tonoplast proteins degraded?
    Marie Maîtrejean
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
    Plant Signal Behav 6:1809-12. 2011
    ..Here, we discuss the possible mechanisms and triggering events involved...
  13. pmc Retention of a bean phaseolin/maize gamma-Zein fusion in the endoplasmic reticulum depends on disulfide bond formation
    Andrea Pompa
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milano, Italy
    Plant Cell 18:2608-21. 2006
    ..We conclude that disulfide bonds that lead to insolubilization are a determinant for PB-mediated protein accumulation in the ER...
  14. ncbi request reprint The phaseolin vacuolar sorting signal promotes transient, strong membrane association and aggregation of the bean storage protein in transgenic tobacco
    Silvana Castelli
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, I 20133 Milano, Italy
    J Exp Bot 56:1379-87. 2005
    ..These results support the hypothesis of a relationship between aggregation and vacuolar sorting of phaseolin and indicate that sorting may start in early compartments of the secretory pathway...
  15. pmc A phaseolin domain involved directly in trimer assembly is a determinant for binding by the chaperone BiP
    Ombretta Foresti
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milano, Italy
    Plant Cell 15:2464-75. 2003
    ..Finally, we show that the other, internal region of contact between monomers is necessary for phaseolin assembly in vivo and contains one potential BiP binding site...
  16. ncbi request reprint Plant endoplasmin supports the protein secretory pathway and has a role in proliferating tissues
    Eva M Klein
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy
    Plant J 48:657-73. 2006
    ..This increase occurs in proliferating rather than expanding leaf cells, indicating an important role for endoplasmin in proliferating plant tissues...
  17. pmc The putative K(+) channel subunit AtKCO3 forms stable dimers in Arabidopsis
    Alessandra Rocchetti
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche Milano, Italy
    Front Plant Sci 3:251. 2012
    ..We conclude that if AtKCO3 ever functions as a K(+) channel, active tetramers are held by particularly weak interactions, are formed only in unknown specific conditions and may require partner proteins...
  18. doi request reprint Traffic routes and signals for the tonoplast
    Emanuela Pedrazzini
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
    Traffic 14:622-8. 2013
    ..The routes can be distinguished based on their sensitivity to drugs such as brefeldin A and C834 as well as using mutant plants that are defective in adaptor proteins of vesicle coats, or dominant-negative mutants of Rab GTPases...
  19. ncbi request reprint Physical methods
    Alessandro Vitale
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy
    Plant Mol Biol 50:825-36. 2002
    ..The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated...
  20. pmc Identification of the protein storage vacuole and protein targeting to the vacuole in leaf cells of three plant species
    Misoon Park
    Center for Plant Intracellular Trafficking, Pohang University of Science and Technology, Pohang, 790 784, Korea
    Plant Physiol 134:625-39. 2004
    ....
  21. doi request reprint Defence-related gene expression in transgenic lemon plants producing an antimicrobial Trichoderma harzianum endochitinase during fungal infection
    Gaetano Distefano
    Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, Via Valdisavoia 5, Catania, 95123, Italy
    Transgenic Res 17:873-9. 2008
    ....
  22. ncbi request reprint A novel C-terminal sequence from barley polyamine oxidase is a vacuolar sorting signal
    Manuela Cervelli
    Dipartimento di Biologia, Universita Roma Tre, Viale Guglielmo Marconi 446, 00146 Rome, Italy
    Plant J 40:410-8. 2004
    ..No consensus sequence or common structural determinant is required for the intracellular retention of the MPAO-T protein, but a gradual lowering of the efficiency was observed as a result of progressive deletion of the C-terminus...
  23. pmc The C-terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its Golgi-mediated sorting to the vacuole
    Jane L Hadlington
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    Mol Biol Cell 14:2592-602. 2003
    ..We also show that unassembled light chains are efficiently secreted as monomers by the plant secretory pathway...
  24. ncbi request reprint C-terminal extension of phaseolin with a short methionine-rich sequence can inhibit trimerisation and result in high instability
    James Nuttall
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Plant Mol Biol 51:885-94. 2003
    ..This indicates that the plant ER quality control machinery can dispose of defective proteins with different kinetics and perhaps mechanisms, depending on the nature of their defect...