Research Topics
| A ScaloniSummaryAffiliation: Istituto di Chimica Biomolecolare Country: Italy Publications
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Detail Information
Publications
Structural investigations on human erythrocyte acylpeptide hydrolase by mass spectrometric proceduresA Scaloni
Centro Internazionale Servizi di Spettrometria di Massa, C N R Universita di Napoli Federico II, Naples, Italy
J Protein Chem 18:349-60. 1999..The site of tryptic hydrolysis was identified as Arg193. The secondary structural organization of the protease domain of the enzyme is consistent with the alpha/beta hydrolase fold...
Structural analysis and disulfide-bridge pairing of two odorant-binding proteins from Bombyx moriA Scaloni
Centro Internazionale Servizi di Spettrometria di Massa IABBAM, National Research Council, Naples, 80131, Italy
Biochem Biophys Res Commun 266:386-91. 1999..This arrangement of disulphide bonds indicates that the three-dimensional structure of insect OBPs is defined by three loops, rich in helical content, which can vary in size and charge distribution from one protein to another...
Probing the reactivity of nucleophile residues in human 2,3-diphosphoglycerate/deoxy-hemoglobin complex by aspecific chemical modificationsA Scaloni
Centro Internazionale Servizi di Spettrometria di Massa, CNR, Napoli, Italy
FEBS Lett 452:190-4. 1999..The results here described indicate that this methodology can be efficiently used in aspecific modification experiments directed to the molecular characterization of the surface topology in proteins and protein complexes...
Purification, cloning and characterisation of odorant- and pheromone-binding proteins from pig nasal epitheliumA Scaloni
I.A.B.B.A.M.-Centro Internazionale Servizi di Spettrometria di Massa National Research Council, Napoli, Italy
Cell Mol Life Sci 58:823-34. 2001....
Glutamate-1-semialdehyde aminotransferase from Sulfolobus solfataricusG Palmieri
Dipartimento di Chimica Organica e Biologica, Napoli, Italy
Biochem J 320:541-5. 1996..Evidence is provided that ALA synthesis in S. solfataricus follows the C5 pathway characteristic of plants, algae, cyanobacteria and many other bacteria...
Bacterial expression and conformational analysis of a chemosensory protein from Schistocerca gregariaD Picone
Dipartimento di Chimica, Universita' Federico II, Napoli, Italy
Eur J Biochem 268:4794-801. 2001....
Amino acid sequence, S-S bridge arrangement and distribution in plant tissues of thionins from Viscum albumS Orru
Centro Internazionale di Servizi di Spettrometria di Massa del CNR, Napoli, Italy
Biol Chem 378:989-96. 1997..The distribution of the new variant toxin in different mistletoe subspecies was investigated. Viscotoxin A1 is abundant in the seeds of the three European subspecies of V. album whereas it represents a minor component in the shoots...
Cloning, post-translational modifications, heterologous expression and ligand-binding of boar salivary lipocalinD Loebel
Institut fur Physiologie, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany
Biochem J 350:369-79. 2000..Binding experiments on natural and recombinant SAL were performed with the fluorescent probe 1-aminoanthracene, which was efficiently displaced by the steroidal sex pheromone, as well as by several odorants...
Acylpeptide hydrolase: inhibitors and some active site residues of the human enzymeA Scaloni
Universita La Sapienza, Dipartimento di Scienze Biochimiche A Rossi Fanelli, Rome, Italy
J Biol Chem 267:3811-8. 1992..These findings suggest that acylpeptidase hydrolase is a member of a family of enzymes with extremely diverse functions...
Amino acid sequence, post-translational modifications, binding and labelling of porcine odorant-binding proteinS Paolini
Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Italy
Chem Senses 23:689-98. 1998..These compounds together with other ligands were able to bind the protein as revealed by competitive binding experiments...
Crystallization and preliminary X-ray studies of human erythrocyte acylpeptide hydrolaseM Feese
Institute of Molecular Biology, University of Oregon, Eugene 97403
J Mol Biol 233:546-9. 1993..One set of crystals, which diffract to 2.2 A, are in space group P2 with cell dimensions a = 118.6 A, b = 82.3 A, c = 182.1 A, beta = 91.6 degrees. The search for suitable heavy-atom derivatives is underway...
Lipocalins of boar salivary glands binding odours and pheromonesS Marchese
Dipartimento di Chimica e Biotecnologie Agrarie, University of Pisa, Italy
Eur J Biochem 252:563-8. 1998..The protein also binds 2-isobutyl-3-methoxypyrazine, a good ligand for most odorant-binding proteins, with a dissociation constant of 5 microM...
Bovine cytosolic 5'-nucleotidase acts through the formation of an aspartate 52-phosphoenzyme intermediateS Allegrini
Dipartimento di Scienze del Farmaco, , via Muroni 23/a, 07100 Sassari Italy
J Biol Chem 276:33526-32. 2001..From sequence alignments we conclude that cytosolic 5'-nucleotidase, along with other nucleotidases, belong to a large superfamily of hydrolases with different substrate specificities and functional roles...
Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificitiesW M Jones
Rockefeller University, New York, NY 10021
Proc Natl Acad Sci U S A 88:2194-8. 1991..There could also be a relationship between the expression of these two enzymes and acetylated peptide growth factors in some carcinomas...
Thiol/disulfide interconversion in bovine lens aldose reductase induced by intermediates of glutathione turnoverP G Vilardo
Dipartimento di Fisiologia e Biochimica, , Via S. Maria 55, 56100 Pisa, Italy
Biochemistry 40:11985-94. 2001..A pathway of thiol/disulfide interconversion for bovine lens ALR2 induced, in oxidative conditions, by physiological thiol compounds is proposed...
Biochemical characterization and bacterial expression of an odorant-binding protein from Locusta migratoriaL Ban
Department of Entomology, China Agricultural University, Beijing, China
Cell Mol Life Sci 60:390-400. 2003..This is the first report of an odorant-binding protein identified and characterized from Orthoptera...
Antimicrobial peptide induction in the haemolymph of the Mexican scorpion Centruroides limpidus limpidus in response to septic injuryR C Rodríguez de la Vega
Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad 2001, Cuernavaca 62210, Mexico
Cell Mol Life Sci 61:1507-19. 2004..limpidus limpidus defensin-like peptide (Cll-dlp). This peptide accumulates in the haemolymph in response to septic injury, independently of transcriptional regulation...
