Affiliation: Istituto di Genetica Molecolare
Brai A, Fazi R, Tintori C, Zamperini C, Bugli F, Sanguinetti M, et al
. Human DDX3 protein is a valuable target to develop broad spectrum antiviral agents. Proc Natl Acad Sci U S A. 2016;113:5388-93 pubmed publisher
..Pharmacokinetics and toxicity studies in rats confirmed a good safety profile and bioavailability of 16d. Thus, DDX3 is here validated as a valuable therapeutic target. ..
Bavagnoli L, Cucuzza S, Campanini G, Rovida F, Paolucci S, Baldanti F, et al
. The novel influenza A virus protein PA-X and its naturally deleted variant show different enzymatic properties in comparison to the viral endonuclease PA. Nucleic Acids Res. 2015;43:9405-17 pubmed publisher
..These results point to a previously undetected role of the last C-ter 20 aa for the catalytic activity of PA-X and support distinct roles for these proteins in the viral life cycle. ..
Mentegari E, Kissova M, Bavagnoli L, Maga G, Crespan E. DNA Polymerases ? and ?: The Double-Edged Swords of DNA Repair. Genes (Basel). 2016;7: pubmed publisher
..This review summarizes the most recent results on the ambivalent properties of these enzymes in limiting or promoting genetic instability in mammalian cells, as well as their potential use as targets for anticancer chemotherapy. ..
Maga G, Villani G, Ramadan K, Shevelev I, Tanguy Le Gac N, Blanco L, et al
. Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis. J Biol Chem. 2002;277:48434-40 pubmed
..Our results suggest that the complex between PCNA and pol lambda may play an important role in the bypass of abasic sites in human cells. ..
Maga G, Blanca G, Shevelev I, Frouin I, Ramadan K, Spadari S, et al
. The human DNA polymerase lambda interacts with PCNA through a domain important for DNA primer binding and the interaction is inhibited by p21/WAF1/CIP1. FASEB J. 2004;18:1743-5 pubmed
..Given the high rate of frameshift mutations induced by pol lambda and its ability to bypass abasic sites, accurate regulation of pol lambda activity by PCNA and p21 concerted action might be important for preventing genetic instability. ..
Maga G, Ramadan K, Locatelli G, Shevelev I, Spadari S, Hubscher U. DNA elongation by the human DNA polymerase lambda polymerase and terminal transferase activities are differentially coordinated by proliferating cell nuclear antigen and replication protein A. J Biol Chem. 2005;280:1971-81 pubmed
Maga G, Shevelev I, Villani G, Spadari S, Hubscher U. Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda. Nucleic Acids Res. 2006;34:1405-15 pubmed
..Possible physiological implications of these findings for the in vivo fidelity of pol lambda are discussed. ..
Maga G, Villani G, Crespan E, Wimmer U, Ferrari E, Bertocci B, et al
. 8-oxo-guanine bypass by human DNA polymerases in the presence of auxiliary proteins. Nature. 2007;447:606-8 pubmed
Maga G, Crespan E, Wimmer U, van Loon B, Amoroso A, Mondello C, et al
. Replication protein A and proliferating cell nuclear antigen coordinate DNA polymerase selection in 8-oxo-guanine repair. Proc Natl Acad Sci U S A. 2008;105:20689-94 pubmed publisher