Luigi Gradoni

Summary

Affiliation: Istituto Superiore di Sanit
Country: Italy

Publications

  1. ncbi request reprint Failure of a multi-subunit recombinant leishmanial vaccine (MML) to protect dogs from Leishmania infantum infection and to prevent disease progression in infected animals
    L Gradoni
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Vaccine 23:5245-51. 2005
  2. pmc Visceral leishmaniasis treatment, Italy
    Luigi Gradoni
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Rome, Italy
    Emerg Infect Dis 9:1617-20. 2003
  3. ncbi request reprint An update on antileishmanial vaccine candidates and prospects for a canine Leishmania vaccine
    L Gradoni
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Vet Parasitol 100:87-103. 2001
  4. doi request reprint Longitudinal study on the detection of canine Leishmania infections by conjunctival swab analysis and correlation with entomological parameters
    Marina Gramiccia
    Unit of Vector borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Vet Parasitol 171:223-8. 2010
  5. doi request reprint The northward spread of leishmaniasis in Italy: evidence from retrospective and ongoing studies on the canine reservoir and phlebotomine vectors
    Michele Maroli
    Istituto Superiore di Sanita, Rome, Italy
    Trop Med Int Health 13:256-64. 2008
  6. ncbi request reprint Evaluation of the Leishmania recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzyme-linked immunosorbent assay
    A Scalone
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Rome, Italy
    Vet Parasitol 104:275-85. 2002
  7. pmc Incidence and time course of Leishmania infantum infections examined by parasitological, serologic, and nested-PCR techniques in a cohort of naive dogs exposed to three consecutive transmission seasons
    Gaetano Oliva
    Unit of Vector borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Clin Microbiol 44:1318-22. 2006
  8. doi request reprint Drug regimens for visceral leishmaniasis in Mediterranean countries
    Luigi Gradoni
    Istituto Superiore di Sanita, Rome, Italy
    Trop Med Int Health 13:1272-6. 2008
  9. ncbi request reprint The current status of zoonotic leishmaniases and approaches to disease control
    Marina Gramiccia
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Int J Parasitol 35:1169-80. 2005
  10. ncbi request reprint Infection of sandflies by a cat naturally infected with Leishmania infantum
    Michele Maroli
    Unit of Vector borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Rome, Italy
    Vet Parasitol 145:357-60. 2007

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Failure of a multi-subunit recombinant leishmanial vaccine (MML) to protect dogs from Leishmania infantum infection and to prevent disease progression in infected animals
    L Gradoni
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Vaccine 23:5245-51. 2005
    ..We conclude that vaccination with MML is not effective to prevent leishmaniasis infection and disease progression in dogs under field conditions...
  2. pmc Visceral leishmaniasis treatment, Italy
    Luigi Gradoni
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Rome, Italy
    Emerg Infect Dis 9:1617-20. 2003
    ..8%, while the proportion of amphotericin B treatments increased from 0% to 97.2%. The countrywide change in therapy is a response to both disease reemergence and increasing antimonial failure...
  3. ncbi request reprint An update on antileishmanial vaccine candidates and prospects for a canine Leishmania vaccine
    L Gradoni
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Vet Parasitol 100:87-103. 2001
    ..Finally, some practical requirements for the evaluation of vaccine candidates in dogs are indicated...
  4. doi request reprint Longitudinal study on the detection of canine Leishmania infections by conjunctival swab analysis and correlation with entomological parameters
    Marina Gramiccia
    Unit of Vector borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Vet Parasitol 171:223-8. 2010
    ..CS n-PCR technique can be a suitable marker for assessing Leishmania exposure in dogs as a non-invasive alternative to current serological and molecular tools...
  5. doi request reprint The northward spread of leishmaniasis in Italy: evidence from retrospective and ongoing studies on the canine reservoir and phlebotomine vectors
    Michele Maroli
    Istituto Superiore di Sanita, Rome, Italy
    Trop Med Int Health 13:256-64. 2008
    ..Northern continental Italy is now focally endemic for VL and a moderate risk for human disease does exist, although the intensity of transmission seems to be lower than in traditional settings of Mediterranean VL...
  6. ncbi request reprint Evaluation of the Leishmania recombinant K39 antigen as a diagnostic marker for canine leishmaniasis and validation of a standardized enzyme-linked immunosorbent assay
    A Scalone
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Rome, Italy
    Vet Parasitol 104:275-85. 2002
    ..The rK39 ELISA is a relatively simple and rapid assay for assessing the infection status of dogs, and is an alternative to IFAT, especially when screening large numbers of samples...
  7. pmc Incidence and time course of Leishmania infantum infections examined by parasitological, serologic, and nested-PCR techniques in a cohort of naive dogs exposed to three consecutive transmission seasons
    Gaetano Oliva
    Unit of Vector borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Clin Microbiol 44:1318-22. 2006
    ....
  8. doi request reprint Drug regimens for visceral leishmaniasis in Mediterranean countries
    Luigi Gradoni
    Istituto Superiore di Sanita, Rome, Italy
    Trop Med Int Health 13:1272-6. 2008
    ..A country's wealth affects the treatment choice, which represents a balance between drug efficacy, toxicity and cost, and costs associated with patient's care...
  9. ncbi request reprint The current status of zoonotic leishmaniases and approaches to disease control
    Marina Gramiccia
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Int J Parasitol 35:1169-80. 2005
    ..Laboratory and field studies have shown improved efficacy of these preparations for both individual and mass protection...
  10. ncbi request reprint Infection of sandflies by a cat naturally infected with Leishmania infantum
    Michele Maroli
    Unit of Vector borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Rome, Italy
    Vet Parasitol 145:357-60. 2007
    ..This is the first evidence of transmissibility of feline parasites to a proven vector, suggesting that cats may represent an additional domestic reservoir for L. infantum...
  11. ncbi request reprint Mapping the main Leishmania phlebotomine vector in the endemic focus of the Mt. Vesuvius in southern Italy
    Erika Rossi
    Dipartimento MIPI, Istituto Superiore di Sanita, Rome, Italy
    Geospat Health 1:191-8. 2007
    ..perniciosus densities between the two surveyed areas...
  12. ncbi request reprint Seasonal phenology, host-blood feeding preferences and natural Leishmania infection of Phlebotomus perniciosus (Diptera, Psychodidae) in a high-endemic focus of canine leishmaniasis in Rome province, Italy
    E Rossi
    Section of Vector Borne Diseases and International Health, MIPI Department, Istituto Superiore di Sanita, Rome, Italy
    Acta Trop 105:158-65. 2008
    ..6%) than the promastigote infection rate determined by microscopy in the dissected females (1.4%). The intense Leishmania transmission in the study area was confirmed by the high prevalence of anti-leishmanial antibodies in dogs (33.3%)...
  13. ncbi request reprint Use of the Plasmodium vivax merozoite surface protein 1 gene sequence analysis in the investigation of an introduced malaria case in Italy
    Carlo Severini
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161, Rome, Italy
    Acta Trop 84:151-7. 2002
    ..some geographical isolates looked tightly clustered (e.g. Korean and Punjab isolates), but others were less so...
  14. ncbi request reprint Evidence for an impact on the incidence of canine leishmaniasis by the mass use of deltamethrin-impregnated dog collars in southern Italy
    M Maroli
    Department of Parasitology, Istituto Superiore di Sanita, Rome, Italy
    Med Vet Entomol 15:358-63. 2001
    ....
  15. doi request reprint Aspects of the bionomics of Phlebotomus sergenti sandflies from an endemic area of anthroponotic cutaneous leishmaniasis in Aleppo Governorate, Syria
    M Maroli
    Section of Vector Borne Diseases and International Health, Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Med Vet Entomol 23:148-54. 2009
    ....
  16. ncbi request reprint Risk of Plasmodium vivax malaria reintroduction in Uzbekistan: genetic characterization of parasites and status of potential malaria vectors in the Surkhandarya region
    Carlo Severini
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Trans R Soc Trop Med Hyg 98:585-92. 2004
    ..An entomological investigation in the area suggested that three Anopheles species, namely A. superpictus, A. pulcherrimus and A. hyrcanus may have a potential role in the endemic transmission of P. vivax...
  17. ncbi request reprint Decreased sensitivity to meglumine antimoniate (Glucantime) of Leishmania infantum isolated from dogs after several courses of drug treatment
    M Gramiccia
    Laboratorio di Parassitologia, , Rome, Italy
    Ann Trop Med Parasitol 86:613-20. 1992
    ..This result underlines the futility and danger of repeated antimonial treatments of dogs with signs of leishmaniasis, as these may produce a permanent reservoir of parasites unsusceptible to the drugs in human clinical use...
  18. ncbi request reprint Kinetics of parasite cysteine proteinase inactivation by NO-donors
    Alessio Bocedi
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Viale Regina Elena 299, I 00161 Rome, Italy
    Biochem Biophys Res Commun 315:710-8. 2004
    ..infantum cysteine proteinase from inactivation by SNAP. These results indicate that parasite cysteine proteinase inactivation by NO-donors occurs via NO-mediated S-nitrosylation of the Cys25 catalytic residue...
  19. pmc Treatment of Mediterranean visceral leishmaniasis
    L Gradoni
    Laboratorio di Parassitologia, , Rome, Italy
    Bull World Health Organ 73:191-7. 1995
    ....
  20. ncbi request reprint NO donors inhibit Leishmania infantum cysteine proteinase activity
    L Salvati
    Dipartimento di Biologia, , Rome, Italy
    Biochim Biophys Acta 1545:357-66. 2001
    ..These data suggest that the L. infantum cysteine proteinase undergoes NO-mediated S-nitrosylation, thereby representing a possible mechanism of antiparasitic host defence...
  21. ncbi request reprint Activity of D-carnitine and its derivatives on Trypanosoma infections in rats and mice
    M Manganaro
    Department of Infectious Diseases, University of Rome La Sapienza, Rome, Italy
    Parasite 10:147-51. 2003
    ..Considering both, lack of toxicity and inhibitory activity, D-carnitine may have a role in the treatment of African trypanosomiasis, in association with available trypanocidal drugs...
  22. ncbi request reprint Case report: effect of antileishmanial treatment on hepatitis C viraemia in a visceral leishmaniasis patient with chronic hepatitis C
    Davide F Precone
    Department of Medicine and Public Health, Unit of Infectious Diseases, Second University of Naples, Naples, Italy
    Trans R Soc Trop Med Hyg 97:559-60. 2003
    ..This case suggests that VL causes an increase in HCV replication, but, although the growth of the HCC can be defined as exceptional, a relationship with VL is not proven...
  23. doi request reprint Extranodal gammadelta-T-cell lymphoma in a dog with leishmaniasis
    Valentina Foglia Manzillo
    Dipartimento di Scienze Cliniche Veterinarie, Facoltà di Medicina Veterinaria Università di Napoli Federico II, Napoli, Italy
    Vet Clin Pathol 37:298-301. 2008
    ..gammadelta-T cells may be involved in the host response to this parasite, and prolonged antigenic stimulation and chronic immunosuppression (typical of leishmaniasis) play a crucial role in the etiopathogenesis of T-cell lymphoma...
  24. doi request reprint Validation of a recombinant based antibody ELISA for diagnosis of human and canine leishmaniasis
    Franco Daprà
    Department of Animal Production, Epidemiology and Ecology, Faculty of Veterinary Medicine, University of Torino, Via Leonardo da Vinci 44, 10095 Grugliasco TO, Italy
    J Immunoassay Immunochem 29:244-56. 2008
    ..44%, and concordance (K) 0.88. The dog panel showed DA 0.998, Sp 99.54%, Se 98.54%, and K 0.98. The proposed method is the best recombinant antigen-based ELISA, and can be used as IFAT substitute for mass screening...
  25. ncbi request reprint Mediterranean visceral leishmaniasis in HIV-negative adults: a retrospective analysis of 64 consecutive cases (1995-2001)
    Pasquale Pagliano
    I Division of Infectious Disease, D Cotugno Hospital, Naples, Italy
    J Antimicrob Chemother 52:264-8. 2003
    ..To evaluate in a retrospective analysis cases of Mediterranean visceral leishmaniasis (VL) diagnosed in HIV-negative adults during a 7-year period...
  26. ncbi request reprint Liposomal amphotericin B for the treatment of visceral leishmaniasis
    Caryn Bern
    Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Clin Infect Dis 43:917-24. 2006
    ..The public health community should work to broaden access to preferential liposomal amphotericin B pricing by public sector VL treatment programs...
  27. ncbi request reprint A review of leishmaniasis in Dalmatia (Croatia) and results from recent surveys on phlebotomine sandflies in three southern counties
    Sanja Bosnić
    Croatian Veterinary Institute, Department of Parasitology, Zagreb, Croatia
    Acta Trop 99:42-9. 2006
    ..Among the species, which may act as Leishmania infantum vectors, P. tobbi and P. neglectus were the most abundant...
  28. ncbi request reprint The efficacy of enrofloxacin, alone or combined with metronidazole, in the therapy of canine leishmaniasis
    Paolo Bianciardi
    DVM, Milano, Via Dell Usignolo 1, 20147, Milan, Italy
    Parasitol Res 93:486-92. 2004
    ....
  29. ncbi request reprint A 6 day course of liposomal amphotericin B in the treatment of infantile visceral leishmaniasis: the Italian experience
    Antonio Cascio
    Clinica Malattie Infettive, Universita di Messina, Messina, Italy
    J Antimicrob Chemother 54:217-20. 2004
    ..To evaluate in a retrospective analysis the efficacy and safety of a 6 day course of liposomal amphotericin B (L-AmB) in infantile cases of Mediterranean visceral leishmaniasis (VL) diagnosed over a 10 year period in Italy...
  30. ncbi request reprint Catalytic properties of cysteine proteinases from Trypanosoma cruzi and Leishmania infantum: a pre-steady-state and steady-state study
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Viale Guglielmo Marconi 446, I 00146 Rome, Italy
    Biochem Biophys Res Commun 309:659-65. 2003
    ..Comparison with the steady-state and pre-steady-state kinetics of homologous plant enzymes suggests that the parasite cysteine proteinase catalytic behavior appears to be of general significance...
  31. ncbi request reprint Inactivation of parasite cysteine proteinases by the NO-donor 4-(phenylsulfonyl)-3-((2-(dimethylamino)ethyl)thio)-furoxan oxalate
    Paolo Ascenzi
    Dipartimento di Biologia and Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochim Biophys Acta 1703:69-77. 2004
    ..infantum cysteine proteinase displays a maximum at about 340 nm. These results indicate that the parasite cysteine proteinase inactivation by SNO-102 occurs via the NO-mediated S-nitrosylation of the Cys25 catalytic residue...
  32. ncbi request reprint Visceral leishmaniasis in pregnancy: a case series and a systematic review of the literature
    Pasquale Pagliano
    I Division of Infectious Diseases, D Cotugno Hospital, Naples
    J Antimicrob Chemother 55:229-33. 2005
    ..Here we report a small series of pregnant women with VL observed in the Neapolitan area over a 7 year period and carry out a systematic review of the literature on this topic...
  33. ncbi request reprint Nitric oxide limits parasite development in vectors and in invertebrate intermediate hosts
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Italy
    IUBMB Life 53:121-3. 2002
    ..Therefore, NO limits Trypanosoma, Plasmodium, and Schistosoma development at all stages of the parasite life cycle...
  34. ncbi request reprint Distinguishing visceral leishmaniasis from intolerance to pegylated interferon-alpha in a thalassemic splenectomized patient treated for chronic hepatitis C
    Pasquale Pagliano
    D Cotugno Hospital, Department of Emergency, 1st Division of Infectious Diseases, Naples, Italy
    Am J Trop Med Hyg 79:9-11. 2008
    ..Symptoms of VL may be difficult to distinguish from the manifestations of Peg-IFN intolerance. We suggest that VL must be suspected in any immunodepressed patient with an unexplained fever and a history of exposure in an endemic area...
  35. pmc Spread of vector-borne diseases and neglect of Leishmaniasis, Europe
    Jean Claude Dujardin
    Instituut voor Tropische Geneeskunde, Antwerp, Belgium
    Emerg Infect Dis 14:1013-8. 2008
    ..Despite this possibility, no coordinated surveillance of the disease exists at the European level. In this review of leishmaniasis importance in Europe, we would like to bridge the gap between research and surveillance and control...
  36. pmc The relationship between leishmaniasis and AIDS: the second 10 years
    Jorge Alvar
    Control of Neglected Tropical Diseases, World Health Organization, 20 Avenue Appia, CH 1211 Geneva 27, Switzerland
    Clin Microbiol Rev 21:334-59, table of contents. 2008
    ..Based on the previous experience of 20 years of coinfection in Europe, this review focuses on the management of Leishmania-HIV-coinfected patients in low-income countries where leishmaniasis is endemic...
  37. ncbi request reprint The anti-parasitic effects of nitric oxide
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Viale G Marconi 446, 1 00146 Roma, Italy
    IUBMB Life 55:573-8. 2003
    ....
  38. pmc Visceral leishmaniasis causes fever and decompensation in patients with cirrhosis
    Pasquale Pagliano
    Gut 56:893-4. 2007
  39. ncbi request reprint Identification of the Plasmodium vivax mdr-like gene (pvmdr1) and analysis of single-nucleotide polymorphisms among isolates from different areas of endemicity
    Sara Brega
    Equipe d Accueil 37 32, Parasitologie, Mycologie Medicale et Pathologie Exotique, Faculte de Medecine, Universite Claude Bernard, Lyon, France
    J Infect Dis 191:272-7. 2005
    ..Nucleotide polymorphisms at 2 codons of the pvmdr1 gene--Y976F and F1076L--were found in 14 of 23 P. vivax isolates from different areas of endemicity, including Thailand, Indonesia, Turkey, Azerbaijan, and French Guyana...