Michela Flego

Summary

Affiliation: Istituto Superiore di Sanit
Country: Italy

Publications

  1. pmc Clinical development of monoclonal antibody-based drugs in HIV and HCV diseases
    Michela Flego
    Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    BMC Med 11:4. 2013
  2. pmc Generation of human scFvs antibodies recognizing a prion protein epitope expressed on the surface of human lymphoblastoid cells
    Michela Flego
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    BMC Biotechnol 7:38. 2007
  3. ncbi request reprint Application of a synthetic phage antibody library (ETH-2) for the isolation of single chain fragment variable (scFv) human antibodies to the pathogenic isoform of the hamster prion protein (HaPrPsc)
    Alessandro Ascione
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Roma, Italy
    Hybridoma (Larchmt) 24:127-32. 2005
  4. pmc Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from yeast
    Alessandra Mallano
    Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, Italy
    BMC Biotechnol 8:68. 2008
  5. ncbi request reprint Genetic construction, expression, and characterization of a single chain anti-CEA antibody fused to cytosine deaminase from yeast
    Silvia Zamboni
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, I 00161 Rome, Italy
    Int J Oncol 32:1245-51. 2008
  6. doi request reprint Human monoclonal antibodies in single chain fragment variable format with potent neutralization activity against influenza virus H5N1
    Alessandro Ascione
    Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Antiviral Res 83:238-44. 2009
  7. ncbi request reprint Isolation and characterization of the human monoclonal antibodies C10 in single-chain fragment variable (scFv) format to glucose oxidase from Aspergillus niger
    Alessandro Ascione
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    Hybrid Hybridomics 23:380-4. 2004
  8. ncbi request reprint Generation of human single-chain antibody to the CD99 cell surface determinant specifically recognizing Ewing's sarcoma tumor cells
    Mara Gellini
    Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299 00161 Rome, Italy
    Curr Pharm Biotechnol 14:449-63. 2013
  9. pmc Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approach
    Michela Flego
    Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    BMC Infect Dis 5:73. 2005
  10. ncbi request reprint Identification by phage display of the linear continuous MRPr1 epitope in the multidrug resistance-associated protein (MRP1)
    Michela Flego
    Laboratory of Immunology, Istituto Superiore di Sanita, Viale R Elena 299, I 00161, Rome, Italy
    Biol Chem 384:139-42. 2003

Detail Information

Publications12

  1. pmc Clinical development of monoclonal antibody-based drugs in HIV and HCV diseases
    Michela Flego
    Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    BMC Med 11:4. 2013
    ..This overview helps to give a clear picture of the efficacy and challenges of the mAbs in the field of these two infectious diseases which have such a global impact...
  2. pmc Generation of human scFvs antibodies recognizing a prion protein epitope expressed on the surface of human lymphoblastoid cells
    Michela Flego
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    BMC Biotechnol 7:38. 2007
    ..In the present article, we show two new human phage antibodies, isolated on recombinant hamster prion protein (rHaPrP)...
  3. ncbi request reprint Application of a synthetic phage antibody library (ETH-2) for the isolation of single chain fragment variable (scFv) human antibodies to the pathogenic isoform of the hamster prion protein (HaPrPsc)
    Alessandro Ascione
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Roma, Italy
    Hybridoma (Larchmt) 24:127-32. 2005
    ....
  4. pmc Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from yeast
    Alessandra Mallano
    Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, Italy
    BMC Biotechnol 8:68. 2008
    ..The selection and characterization of a human monoclonal antibody in single chain fragment (scFv) format represents a powerful reagent to allow in in vitro and in vivo detection of CD expression in GDEPT/ADEPT studies...
  5. ncbi request reprint Genetic construction, expression, and characterization of a single chain anti-CEA antibody fused to cytosine deaminase from yeast
    Silvia Zamboni
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, I 00161 Rome, Italy
    Int J Oncol 32:1245-51. 2008
    ..This study shows the feasibility of constructing fusion proteins in a prokaryotic cell based system consisting of a human scFv antibody and yCD to convert the antifungal agent 5-FC to 5-FU, one of the widely used anticancer agent...
  6. doi request reprint Human monoclonal antibodies in single chain fragment variable format with potent neutralization activity against influenza virus H5N1
    Alessandro Ascione
    Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Antiviral Res 83:238-44. 2009
    ....
  7. ncbi request reprint Isolation and characterization of the human monoclonal antibodies C10 in single-chain fragment variable (scFv) format to glucose oxidase from Aspergillus niger
    Alessandro Ascione
    Section of Pharmacogenetics, Drug Resistance and Experimental Therapeutics, Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    Hybrid Hybridomics 23:380-4. 2004
    ..Finally, the cDNA sequence of this human scFv may be exploited to generate bispecific antibodies to target in the tumor environment-specific toxic enzymatic reaction...
  8. ncbi request reprint Generation of human single-chain antibody to the CD99 cell surface determinant specifically recognizing Ewing's sarcoma tumor cells
    Mara Gellini
    Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Viale Regina Elena 299 00161 Rome, Italy
    Curr Pharm Biotechnol 14:449-63. 2013
    ..Thanks to its properties the new anti-CD99 antibody here described represents the first step towards the construction of new selective ES therapeutics...
  9. pmc Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approach
    Michela Flego
    Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    BMC Infect Dis 5:73. 2005
    ....
  10. ncbi request reprint Identification by phage display of the linear continuous MRPr1 epitope in the multidrug resistance-associated protein (MRP1)
    Michela Flego
    Laboratory of Immunology, Istituto Superiore di Sanita, Viale R Elena 299, I 00161, Rome, Italy
    Biol Chem 384:139-42. 2003
    ....
  11. ncbi request reprint [Use of phage libraries for the in vitro production of recombinant monoclonal antibodies of predetermined specificity]
    Michela Flego
    Laboratorio di Immunologia, Istituto Superiore di Sanita, V le Regina Elena 299, 00161 Roma
    Ann Ist Super Sanita 38:401-10. 2002
    ....
  12. pmc Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein
    Emiliano Pavoni
    Kenton Labs, c o Sigma Tau, Via Pontina, km 30, 400, 00040 Pomezia RM, Italy
    BMC Cancer 6:41. 2006
    ..The selection and characterization of human anti-CEA single-chain antibody fragments (scFv) is a first step toward the construction of new anticancer monoclonal antibodies designed for optimal blood clearance and tumor penetration...