Emilio Ciusani

Summary

Affiliation: Istituto Nazionale Neurologico Carlo Besta
Country: Italy

Publications

  1. ncbi request reprint In vitro effects of topotecan and ionizing radiation on TRAIL/Apo2L-mediated apoptosis in malignant glioma
    Emilio Ciusani
    Department of Clinical Investigation, National Neurological Institute C Besta, Via Celoria, Milan, Italy
    J Neurooncol 71:19-25. 2005
  2. ncbi request reprint Valproic acid increases the in vitro effects of nitrosureas on human glioma cell lines
    Emilio Ciusani
    Department of Clinical Investigation, Neurological National Institute Carlo Besta, Milan, Italy
    Oncol Res 16:453-63. 2007
  3. doi request reprint Circulating T regulatory cells migration and phenotype in glioblastoma patients: an in vitro study
    Chiara Vasco
    Laboratory of Clinical Pathology and Medical Genetics, Foundation IRCCS Neurological Institute C Besta, Via Celoria, 11, 20133, Milan, Italy
    J Neurooncol 115:353-63. 2013
  4. doi request reprint Isolation and expansion of human and mouse brain microvascular endothelial cells
    Stefania E Navone
    Laboratory of Cellular Neurobiology, Istituto Di Ricovero e Cura a Carattere Scientifico Foundation Neurological Institute Carlo Besta, Milan, Italy
    Nat Protoc 8:1680-93. 2013
  5. ncbi request reprint Effect of association of temozolomide with other chemotherapic agents on cell growth inhibition in glioma cell lines
    Marco Balzarotti
    Laboratory of Clinical Investigation, Department of Neurology, National Neurological Institute C Besta Via Celoria, Milan, Italy
    Oncol Res 14:325-30. 2004
  6. doi request reprint In vitro effects of Cyberknife-driven intermittent irradiation on glioblastoma cell lines
    Alessandra Canazza
    Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico C Besta, Milan, Italy
    Neurol Sci 32:579-88. 2011
  7. ncbi request reprint Production and post-surgical modification of VEGF, tPA and PAI-1 in patients with glioma
    Andrea Salmaggi
    National Neurological Institute C Besta, Milan, Italy
    Cancer Biol Ther 5:204-9. 2006
  8. doi request reprint A mesenchymal stromal cell line resistant to paclitaxel that spontaneously differentiates into osteoblast-like cells
    Augusto Pessina
    Department of Public Health Microbiology Virology, University of Milan, Via Pascal 36, 20133, Milan, Italy
    Cell Biol Toxicol 27:169-80. 2011
  9. doi request reprint Mesenchymal stromal cells primed with Paclitaxel attract and kill leukaemia cells, inhibit angiogenesis and improve survival of leukaemia-bearing mice
    Augusto Pessina
    Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
    Br J Haematol 160:766-78. 2013
  10. doi request reprint Prospective study of carmustine wafers in combination with 6-month metronomic temozolomide and radiation therapy in newly diagnosed glioblastoma: preliminary results
    Andrea Salmaggi
    Fondazione IRCCS Istituto Neurologico C Besta, Milan, Italy
    J Neurosurg 118:821-9. 2013

Collaborators

Detail Information

Publications34

  1. ncbi request reprint In vitro effects of topotecan and ionizing radiation on TRAIL/Apo2L-mediated apoptosis in malignant glioma
    Emilio Ciusani
    Department of Clinical Investigation, National Neurological Institute C Besta, Via Celoria, Milan, Italy
    J Neurooncol 71:19-25. 2005
    ..In our experimental conditions, irradiation with 2 Gy had a modest additive effect on TRAIL-dependent apoptosis and was not able to modulate TRAIL receptor expression...
  2. ncbi request reprint Valproic acid increases the in vitro effects of nitrosureas on human glioma cell lines
    Emilio Ciusani
    Department of Clinical Investigation, Neurological National Institute Carlo Besta, Milan, Italy
    Oncol Res 16:453-63. 2007
    ....
  3. doi request reprint Circulating T regulatory cells migration and phenotype in glioblastoma patients: an in vitro study
    Chiara Vasco
    Laboratory of Clinical Pathology and Medical Genetics, Foundation IRCCS Neurological Institute C Besta, Via Celoria, 11, 20133, Milan, Italy
    J Neurooncol 115:353-63. 2013
    ..Though we provide evidence that CCL2 is implicated in Treg migration, other factors are needed as well to provide such effect. ..
  4. doi request reprint Isolation and expansion of human and mouse brain microvascular endothelial cells
    Stefania E Navone
    Laboratory of Cellular Neurobiology, Istituto Di Ricovero e Cura a Carattere Scientifico Foundation Neurological Institute Carlo Besta, Milan, Italy
    Nat Protoc 8:1680-93. 2013
    ..The entire technique requires ∼7 h of active time. Endothelial cell clusters are readily visible after 48 h, and expansion of BMVECs occurs over the course of ∼60 d. ..
  5. ncbi request reprint Effect of association of temozolomide with other chemotherapic agents on cell growth inhibition in glioma cell lines
    Marco Balzarotti
    Laboratory of Clinical Investigation, Department of Neurology, National Neurological Institute C Besta Via Celoria, Milan, Italy
    Oncol Res 14:325-30. 2004
    ..In conclusion, our results confirm the potential effect of association of chemotherapic drugs with different mechanisms of action in the treatment of gliomas...
  6. doi request reprint In vitro effects of Cyberknife-driven intermittent irradiation on glioblastoma cell lines
    Alessandra Canazza
    Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico C Besta, Milan, Italy
    Neurol Sci 32:579-88. 2011
    ..Although the two cell lines differed in DNA repair capability, the role of Ku70/80 and Rad51 in the cell line radiosensitivity seemed marginal...
  7. ncbi request reprint Production and post-surgical modification of VEGF, tPA and PAI-1 in patients with glioma
    Andrea Salmaggi
    National Neurological Institute C Besta, Milan, Italy
    Cancer Biol Ther 5:204-9. 2006
    ..The great amount of VEGF, tPA and PAI-1 produced by glioma could influence peripheral levels of these molecules. The partial resection of the tumor by surgery was not able to decrease plasma levels of these molecules...
  8. doi request reprint A mesenchymal stromal cell line resistant to paclitaxel that spontaneously differentiates into osteoblast-like cells
    Augusto Pessina
    Department of Public Health Microbiology Virology, University of Milan, Via Pascal 36, 20133, Milan, Italy
    Cell Biol Toxicol 27:169-80. 2011
    ..Taken together, our results indicated that SR-4987 line is a very interesting cell model useful to investigate both drug sensitivity resistance and physiopathological aspects related to mesenchymal cell function...
  9. doi request reprint Mesenchymal stromal cells primed with Paclitaxel attract and kill leukaemia cells, inhibit angiogenesis and improve survival of leukaemia-bearing mice
    Augusto Pessina
    Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
    Br J Haematol 160:766-78. 2013
    ..Once the effectiveness of hMSCsPTX on established cancers in mice is proven, this procedure could be proposed for leukaemia therapy in humans...
  10. doi request reprint Prospective study of carmustine wafers in combination with 6-month metronomic temozolomide and radiation therapy in newly diagnosed glioblastoma: preliminary results
    Andrea Salmaggi
    Fondazione IRCCS Istituto Neurologico C Besta, Milan, Italy
    J Neurosurg 118:821-9. 2013
    ..A combination of carmustine wafers with the Stupp treatment regimen has only been investigated in retrospective studies...
  11. ncbi request reprint CXCL12 in malignant glial tumors: a possible role in angiogenesis and cross-talk between endothelial and tumoral cells
    Andrea Salmaggi
    Istituto Nazionale Neurologico Carlo Besta, Milan, Italy
    J Neurooncol 67:305-17. 2004
    ..Our data support in vivo studies suggesting a role in angiogenesis played by CXCL12, which could represent a possible prognostic factor...
  12. ncbi request reprint In vitro study of low molecular weight heparin effect on cell growth and cell invasion in primary cell cultures of high-grade gliomas
    Marco Balzarotti
    Laboratory of Clinical Investigation, Department of Neurology, Department of Neurosurgery, National Neurological Institute C Besta, Via Celoria 11, 20133 Milan, Italy
    Oncol Res 16:245-50. 2006
    ..In conclusion, our results confirm the antineoplastic effect of LMWH, suggesting a potential direct role on tumor cell growth in high grade gliomas...
  13. pmc Mesenchymal stromal cells primed with paclitaxel provide a new approach for cancer therapy
    Augusto Pessina
    Department of Public Health, Microbiology, Virology, University of Milan, Milan, Italy
    PLoS ONE 6:e28321. 2011
    ....
  14. doi request reprint Intrathecal synthesis of tumor markers is a highly sensitive test in the diagnosis of leptomeningeal metastasis from solid cancers
    Elena Corsini
    Laboratory of Clinical Investigations, Fondazione Istituto Neurologico C Besta, Milan, Italy
    Clin Chem Lab Med 47:874-9. 2009
    ..Data from the literature suggest that measurement of tumor markers (TM) in CSF may be helpful for improving the diagnosis...
  15. doi request reprint Human CD14+ cells loaded with Paclitaxel inhibit in vitro cell proliferation of glioblastoma
    Arianna Bonomi
    Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
    Cytotherapy 17:310-9. 2015
    ..On the basis of these results, we here investigated whether both CD14+ and their derived DCs may behave like mesenchymal stromal cells acquiring anti-tumor activity on priming with PTX...
  16. ncbi request reprint Expression of the new CXCL12 receptor, CXCR7, in gliomas
    Chiara Calatozzolo
    Neuropathology Unit, Istituto Nazionale Neurologico Carlo Besta, Milan, Italy
    Cancer Biol Ther 11:242-53. 2011
    ....
  17. doi request reprint Three-dimensional self-organizing neural architectures: a neural stem cells reservoir and a system for neurodevelopmental studies
    Silvia Cristini
    Laboratory of Cellular Neurobiology, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy
    Tissue Eng Part C Methods 17:1109-20. 2011
    ....
  18. doi request reprint Gemcitabine-releasing mesenchymal stromal cells inhibit in vitro proliferation of human pancreatic carcinoma cells
    Arianna Bonomi
    Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
    Cytotherapy 17:1687-95. 2015
    ..On the basis of these observations, we evaluated the ability of MSCs (from bone marrow and pancreas) to uptake and release gemcitabine (GCB), a drug widely used in pCa treatment...
  19. pmc Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival
    Stefania Elena Navone
    Laboratory of Cellular Neurobiology, Cerebrovascular Diseases Unit, IRCCS Foundation Neurological Institute C, Besta, Via Celoria 11, Milan, 20133, Italy
    Vasc Cell 5:10. 2013
    ..Our goal was to study the efficiency of our pending patent medium, Endothelial Proliferation Medium (EndoPM), on the isolation and purification of human and murine BMVECs...
  20. doi request reprint Expression of neural and neurotrophic markers in nucleus pulposus cells isolated from degenerated intervertebral disc
    Stefania E Navone
    The Cellular Neurobiology Laboratory, Cerebrovascular Diseases Unit, IRCCS Foundation Neurological Institute C Besta, Via Celoria, 11 20133 Milan, Italy
    J Orthop Res 30:1470-7. 2012
    ..These data could improve our knowledge about IVD cellularity and eventually lead to the development of pharmacological therapies...
  21. ncbi request reprint Genetic and plasma markers of venous thromboembolism in patients with high grade glioma
    Francesca L Sciacca
    National Neurological Institute C Besta, Milan, Italy
    Clin Cancer Res 10:1312-7. 2004
    ..We investigated genetic and plasma factors possibly associated with risk of DVT/PE in patients with high-grade glioma...
  22. doi request reprint Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition
    Paola Perego
    Molecular Pharmacology Unit, Dept Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy
    Biochem Pharmacol 83:27-36. 2012
    ..These findings may be relevant to define novel therapeutic strategies...
  23. ncbi request reprint Glioblastoma-derived tumorospheres identify a population of tumor stem-like cells with angiogenic potential and enhanced multidrug resistance phenotype
    Andrea Salmaggi
    Istituto Nazionale Neurologico Carlo Besta, Via Celoria 11, 20133 Milan, Italy
    Glia 54:850-60. 2006
    ..A better understanding of the features of these cell subsets may favor the development of more specifically targeted therapies...
  24. doi request reprint Cell-mediated drug delivery by gingival interdental papilla mesenchymal stromal cells (GinPa-MSCs) loaded with paclitaxel
    Anna Teresa Brini
    a Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy
    Expert Opin Drug Deliv 13:789-98. 2016
    ..We isolated GinPa-MSCs and verified their ability to uptake/release the anticancer agent Paclitaxel (PTX)...
  25. pmc AAV-mediated liver-specific MPV17 expression restores mtDNA levels and prevents diet-induced liver failure
    Emanuela Bottani
    Unit of Molecular Neurogenetics, The Foundation Carlo Besta Institute of Neurology IRCCS, Milan, Italy
    Mol Ther 22:10-7. 2014
    ..These results open new therapeutic perspectives for the treatment of MPV17-related liver-specific MDS...
  26. pmc Drug-releasing mesenchymal cells strongly suppress B16 lung metastasis in a syngeneic murine model
    Augusto Pessina
    Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via Pascal 36, Milan, 20133, Italy
    J Exp Clin Cancer Res 34:82. 2015
    ..We have recently demonstrated that MSCs, upon in vitro priming with anti-cancer drug, become drug-releasing mesenchymal cells (Dr-MCs) able to strongly inhibit cancer cells growth...
  27. ncbi request reprint Fas/CD95-mediated apoptosis in human glioblastoma cells: a target for sensitisation to topoisomerase I inhibitors
    Emilio Ciusani
    Istituto Neurologico C Besta, 20133, Milan, Italy
    Biochem Pharmacol 63:881-7. 2002
    ..The observed effects might have important therapeutic implications for the design of novel therapeutic strategies in the treatment of malignant gliomas...
  28. ncbi request reprint CD4+CD25+ regulatory T cells specific for a thymus-expressed antigen prevent the development of anaphylaxis to self
    Stefano Scabeni
    Immunology and Muscular Pathology Unit, Neurological Institute Foundation Carlo Besta, Milan, Italy
    J Immunol 180:4433-40. 2008
    ..These data indicate that naturally occurring CD4(+)CD25(+) Tregs specific for a peptide expressed under physiological conditions in the thymus are able to suppress the development of a systemic allergic reaction to self...
  29. doi request reprint CD45+/CD133+ positive cells expanded from umbilical cord blood expressing PDX-1 and markers of pluripotency
    Augusto Pessina
    Department of Public Health, Microbiology and Virology, University of Milan, Milan, Italy
    Cell Biol Int 34:783-90. 2010
    ....
  30. pmc Human fetal aorta contains vascular progenitor cells capable of inducing vasculogenesis, angiogenesis, and myogenesis in vitro and in a murine model of peripheral ischemia
    Gloria Invernici
    Neurobiology and Neuroregenerative Therapies Unit, Carlo Besta Neurological Institute, Milan 20133, Italy
    Am J Pathol 170:1879-92. 2007
    ..We conclude that fetal aorta represents an important source for the investigation of the phenotypic and functional features of human vascular progenitor cells...
  31. ncbi request reprint Sensitization to gimatecan-induced apoptosis by tumor necrosis factor-related apoptosis inducing ligand in prostate carcinoma cells
    Paola Perego
    Preclinical Chemotherapy and Pharmacology Unit, Department of Experimental Oncology and Laboratories, Istituto Nazionale per lo Studio e la Cura dei Tumori, Via Venezian 1, 20133 Milan, Italy
    Biochem Pharmacol 71:791-8. 2006
    ..These observations support that the activation of multiple signals could enhance apoptotic response and suggest the therapeutic interest of the TRAIL/camptothecin combination...
  32. ncbi request reprint Expression and modulation of IFN-gamma-inducible chemokines (IP-10, Mig, and I-TAC) in human brain endothelium and astrocytes: possible relevance for the immune invasion of the central nervous system and the pathogenesis of multiple sclerosis
    Andrea Salmaggi
    Istituto Nazionale Neurologico Carlo Besta, I 20133 Milan, Italy
    J Interferon Cytokine Res 22:631-40. 2002
    ..Our data highlight the capability of cells of the CNS to activate the chemoattractant machinery in a proinflammatory environment and in MS...
  33. ncbi request reprint An effect of the PAI-1 4G/5G polymorphism on cholesterol levels may explain conflicting associations with myocardial infarction and stroke
    Giorgio B Boncoraglio
    Cerebrovascular Diseases Unit, Istituto Nazionale Neurologico Carlo Besta, Milano, Italy
    Cerebrovasc Dis 22:191-5. 2006
    ..The 4G/4G genotype is associated with increased risk of myocardial infarction but paradoxically protects against stroke. We hypothesized that this paradox may be explained via an effect of the PAI-1 polymorphism on plasma lipids...
  34. doi request reprint Homocysteine plasma levels in patients with migraine with aura
    Franca Moschiano
    L Mandic Hospital, Via L Mandic 1, Merate LC, Italy
    Neurol Sci 29:S173-5. 2008
    ..Hyperhomocysteinaemia may be a link between MA and ischaemic stroke...