Alessandra Carattoli

Summary

Affiliation: Istituto Superiore di Sanit
Country: Italy

Publications

  1. ncbi request reprint Identification of plasmids by PCR-based replicon typing
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Microbiol Methods 63:219-28. 2005
  2. doi request reprint The genomics of Acinetobacter baumannii: insights into genome plasticity, antimicrobial resistance and pathogenicity
    Francesco Imperi
    Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Rome, Italy
    IUBMB Life 63:1068-74. 2011
  3. pmc Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum β-lactamases
    Belén Ruiz del Castillo
    Service of Microbiology, Hospital Universitario Marqués de Valdecilla IFIMAV, Av de Valdecilla s n, Santander 39008, Spain
    BMC Microbiol 13:84. 2013
  4. doi request reprint Plasmids and the spread of resistance
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Int J Med Microbiol 303:298-304. 2013
  5. doi request reprint Plasmids in Gram negatives: molecular typing of resistance plasmids
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Int J Med Microbiol 301:654-8. 2011
  6. pmc Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis
    Florigio Lista
    Army Medical Research Center, Via Santo Stefano Rotondo 4 00184 Rome Italy
    BMC Microbiol 6:33. 2006
  7. pmc In vitro activity of tigecycline and comparators against carbapenem-susceptible and resistant Acinetobacter baumannii clinical isolates in Italy
    Maria Lina Mezzatesta
    Department of Microbiological and Gynaecological Sciences, University of Catania, Italy
    Ann Clin Microbiol Antimicrob 7:4. 2008
  8. doi request reprint Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 65:2070-5. 2010
  9. pmc Replicon typing of plasmids encoding resistance to newer beta-lactams
    Alessandra Carattoli
    Department of Infectious, Parasituc, Immune Mediated diseases, Istituto Superiore di Sanita, Rome, Italy
    Emerg Infect Dis 12:1145-8. 2006
  10. pmc Molecular epidemiology of Escherichia coli producing extended-spectrum beta-lactamases isolated in Rome, Italy
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Clin Microbiol 46:103-8. 2008

Detail Information

Publications65

  1. ncbi request reprint Identification of plasmids by PCR-based replicon typing
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Microbiol Methods 63:219-28. 2005
    ..These results suggest that the method is potentially applicable to a large number of strains to trace the diffusion of specific multi-drug resistance plasmids in different environments...
  2. doi request reprint The genomics of Acinetobacter baumannii: insights into genome plasticity, antimicrobial resistance and pathogenicity
    Francesco Imperi
    Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Rome, Italy
    IUBMB Life 63:1068-74. 2011
    ..A. baumannii has a high capacity to acquire new genetic determinants and displays an open pan genome; this feature may have played a crucial role in the evolution of this human opportunistic pathogen towards clinical success...
  3. pmc Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum β-lactamases
    Belén Ruiz del Castillo
    Service of Microbiology, Hospital Universitario Marqués de Valdecilla IFIMAV, Av de Valdecilla s n, Santander 39008, Spain
    BMC Microbiol 13:84. 2013
    ..Rep-PCR was used to determine the clonal relationship of the organisms. Plasmids were classified according to their incompatibility. Class 1 and Class 2 integrons and antibiotic resistance genes were analysed by PCR and sequencing...
  4. doi request reprint Plasmids and the spread of resistance
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Int J Med Microbiol 303:298-304. 2013
    ..The characteristics of the most successful plasmids that were at the origin of the spread of carbapenemase, expanded-spectrum β-lactamase, and plasmid-mediated quinolone resistance genes are discussed in this review. ..
  5. doi request reprint Plasmids in Gram negatives: molecular typing of resistance plasmids
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Int J Med Microbiol 301:654-8. 2011
    ..The recognition of successful plasmids is an essential first step to design intervention strategies preventing their spread...
  6. pmc Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis
    Florigio Lista
    Army Medical Research Center, Via Santo Stefano Rotondo 4 00184 Rome Italy
    BMC Microbiol 6:33. 2006
    ..However in an emergency situation, faster genotyping and access to representative databases is necessary...
  7. pmc In vitro activity of tigecycline and comparators against carbapenem-susceptible and resistant Acinetobacter baumannii clinical isolates in Italy
    Maria Lina Mezzatesta
    Department of Microbiological and Gynaecological Sciences, University of Catania, Italy
    Ann Clin Microbiol Antimicrob 7:4. 2008
    ..One hundred and seven clinically significant strains of A. baumannii isolates were included in this study to determine the in vitro activity of tigecycline and comparator agents...
  8. doi request reprint Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 65:2070-5. 2010
    ..We determined the complete nucleotide sequence of pKOX105, a 54 641 bp plasmid from a Klebsiella oxytoca strain that was isolated from a resident of a long-term-care facility in Bolzano, Italy...
  9. pmc Replicon typing of plasmids encoding resistance to newer beta-lactams
    Alessandra Carattoli
    Department of Infectious, Parasituc, Immune Mediated diseases, Istituto Superiore di Sanita, Rome, Italy
    Emerg Infect Dis 12:1145-8. 2006
    ....
  10. pmc Molecular epidemiology of Escherichia coli producing extended-spectrum beta-lactamases isolated in Rome, Italy
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Clin Microbiol 46:103-8. 2008
    ..coli species. The analysis of related bacteria suggests a probable interpatient transmission occurring in several wards, causing small outbreaks...
  11. ncbi request reprint Plasmid-mediated antimicrobial resistance in Salmonella enterica
    Alessandra Carattoli
    Lab Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Curr Issues Mol Biol 5:113-22. 2003
    ..The evolution of a plasmid through the acquisition of integrons is reported, describing novel mechanisms for short-term accumulation of resistance determinants in plasmids circulating in Salmonella...
  12. ncbi request reprint Animal reservoirs for extended spectrum beta-lactamase producers
    A Carattoli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Clin Microbiol Infect 14:117-23. 2008
    ..The increasing occurrence of ESBL producers in animals is highlighted and discussed in this review with respect to the circulation of these resistance traits also among human pathogens...
  13. ncbi request reprint Use of a LightCycler gyrA mutation assay for identification of ciprofloxacin-resistant Campylobacter coli
    Alessandra Carattoli
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    FEMS Microbiol Lett 214:87-93. 2002
    ..This gyrA mutation assay allows a rapid and reproducible screening method of ciprofloxacin-resistant C. coli strains...
  14. pmc Antibiotic resistance genes and Salmonella genomic island 1 in Salmonella enterica serovar Typhimurium isolated in Italy
    Alessandra Carattoli
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 46:2821-8. 2002
    ..The presence of SGI1 was also observed in serovar Typhimurium strains belonging to other phage types, suggesting either the potential mobility of this genomic island or changes in the phage-related phenotype of DT104 strains...
  15. ncbi request reprint Cytotoxin-associated gene A and vacuolating cytotoxin A in human isolates of Helicobacter pylori and their association with the clinical status of ulcer disease
    A Carattoli
    Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanita, Rome, Italy
    Eur J Gastroenterol Hepatol 12:1207-13. 2000
    ..The aim of this study was to evaluate whether different Helicobacter pylori genotypes are associated with different clinical stages of peptic ulcer disease (PUD)...
  16. ncbi request reprint Importance of integrons in the diffusion of resistance
    A Carattoli
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Vet Res 32:243-59. 2001
    ....
  17. pmc Expanding drug resistance through integron acquisition by IncFI plasmids of Salmonella enterica Typhimurium
    A Carattoli
    Istituto Superiore di Sanita, Rome, Italy
    Emerg Infect Dis 7:444-7. 2001
    ..Molecular and genetic evidence indicates that IncFI plasmids are evolving through sequential acquisition of integrons carrying different arrays of antibiotic- resistance genes...
  18. doi request reprint Multilocus sequence typing of IncI1 plasmids carrying extended-spectrum beta-lactamases in Escherichia coli and Salmonella of human and animal origin
    Aurora García-Fernández
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 61:1229-33. 2008
    ..Plasmid multilocus sequence typing (pMLST) was developed to discern naturally occurring IncI1 plasmids in homogeneous groups according to their allele assortment...
  19. ncbi request reprint Outbreak of Acinetobacter baumannii producing the carbapenem-hydrolyzing oxacillinase OXA-58 in Rome, Italy
    Alessandra Giordano
    Department of Science and Public Health, Microbiology Unit, University of Rome La Sapienza, Rome, Italy
    Microb Drug Resist 13:37-43. 2007
    ..All the carbapenem resistant strains analyzed in this study carried the bla (OXA-58) gene located on plasmids...
  20. doi request reprint Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella
    Aurora García-Fernández
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 63:274-81. 2009
    ..The identification of plasmids may help to follow the dissemination of these resistance genes in different countries and environments...
  21. pmc First report of plasmid-mediated quinolone resistance determinant qnrS1 in an Escherichia coli strain of animal origin in Italy
    Marina Cerquetti
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, Italy
    Antimicrob Agents Chemother 53:3112-4. 2009
    ..These elements suggest genetic exchanges among Salmonella and E. coli and a potential animal reservoir for the qnr genes...
  22. pmc Multicopy blaOXA-58 gene as a source of high-level resistance to carbapenems in Acinetobacter baumannii
    Alessia Bertini
    Department of Infectious, Parasitic, Immune Mediated diseases, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 51:2324-8. 2007
    ....
  23. pmc Tetracycline and streptomycin resistance genes, transposons, and plasmids in Salmonella enterica isolates from animals in Italy
    Cristina Pezzella
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 48:903-8. 2004
    ..Most of the tet(A) genes were localized within a deleted Tn1721 transposon variant. Two prevalent repN and repI1 resistance plasmids were identified in Salmonella isolates of our collection...
  24. doi request reprint Multilocus sequence typing of IncN plasmids
    Aurora García-Fernández
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 66:1987-91. 2011
    ..A plasmid multilocus sequence typing (pMLST) scheme was developed for rapid categorization of IncN plasmids...
  25. pmc Klebsiella pneumoniae ST258 producing KPC-3 identified in italy carries novel plasmids and OmpK36/OmpK35 porin variants
    Aurora García-Fernández
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 56:2143-5. 2012
    ....
  26. doi request reprint Plasmid double locus sequence typing for IncHI2 plasmids, a subtyping scheme for the characterization of IncHI2 plasmids carrying extended-spectrum beta-lactamase and quinolone resistance genes
    Aurora García-Fernández
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 65:1155-61. 2010
    ..We analysed the available IncHI2 whole DNA plasmid sequences to devise a rapid typing scheme to categorize the members of this plasmid family into homogeneous groups...
  27. doi request reprint Plasmid-mediated quinolone resistance and β-lactamases in Escherichia coli from healthy animals from Nigeria
    Daniela Fortini
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 66:1269-72. 2011
    ....
  28. ncbi request reprint Detection of resistance to rifampicin and decreased susceptibility to penicillin in Neisseria meningitidis by real-time multiplex polymerase chain reaction assay
    Paola Stefanelli
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, 00161 Rome, Italy
    Diagn Microbiol Infect Dis 58:241-4. 2007
    ..The method was applied to N. meningitidis strains and to culture-negative cerebrospinal fluids (CSFs) from patients with meningococcal invasive disease. A spiked CSF was used to determine the sensitivity of the assay...
  29. doi request reprint Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes
    Laura Villa
    Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 67:1645-50. 2012
    ....
  30. pmc Characterization and PCR-based replicon typing of resistance plasmids in Acinetobacter baumannii
    Alessia Bertini
    Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 54:4168-77. 2010
    ..We showed that this conjugative plasmid type was widely diffused in our collection, likely representing the most important vehicle promoting the horizontal transmission of A. baumannii resistance plasmids...
  31. doi request reprint Detection of gyrA and gyrB mutations in Clostridium difficile isolates by real-time PCR
    Patrizia Spigaglia
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Mol Cell Probes 24:61-7. 2010
    ..The results obtained indicate that this real-time PCR assay is a rapid, reproducible and accurate screening method of the predominant mutations determining FQ-resistance in C. difficile strains...
  32. pmc Multidrug and broad-spectrum cephalosporin resistance among Salmonella enterica serotype enteritidis clinical isolates in southern Italy
    Laura Villa
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    J Clin Microbiol 40:2662-5. 2002
    ..Five isolates produced SHV-12, and one isolate encoded a class C beta-lactamase. The bla(SHV-12) gene was located in at least two different self-transferable plasmids, one of which also carried a novel class 1 integron...
  33. pmc Extended-spectrum beta-lactamases in Escherichia coli isolated from dogs and cats in Rome, Italy, from 2001 to 2003
    Alessandra Carattoli
    Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 49:833-5. 2005
    ..coli from sick and healthy dogs and cats. Molecular characterization suggests the presence of several combinations of beta-lactamase genes in E. coli from companion animals...
  34. doi request reprint Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants
    Laura Villa
    Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 65:2518-29. 2010
    ..We devised a rapid sequence-based typing scheme to categorize the members of this plasmid family into homogeneous groups...
  35. pmc Complete sequence of the IncT-type plasmid pT-OXA-181 carrying the blaOXA-181 carbapenemase gene from Citrobacter freundii
    Laura Villa
    Department of Infectious, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 57:1965-7. 2013
    ..6-kb ColE-type and blaOXA-181-bearing plasmid recently identified in a Klebsiella pneumoniae isolate. However, in both plasmids, insertion sequence ISEcp1 might have played a role in acquisition of the blaOXA-181 gene...
  36. pmc An ertapenem-resistant extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae clone carries a novel OmpK36 porin variant
    Aurora García-Fernández
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 54:4178-84. 2010
    ..pneumoniae isolates derived from patients from 2006 to 2009 demonstrated the high frequency of this gene variant as well as its association with ertapenem resistance, reduced susceptibility to meropenem, and susceptibility to imipenem...
  37. ncbi request reprint Identification of ciprofloxacin-resistant Campylobacter jejuni and analysis of the gyrA gene by the LightCycler mutation assay
    Anna Maria Dionisi
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Mol Cell Probes 18:255-61. 2004
    ..This gyrA mutation assay allows a rapid and reproducible screening method of ciprofloxacin resistant strains and was applied to C. jejuni strains isolated in Italy in 2000...
  38. ncbi request reprint Detection and characterization of class 1 integrons in enterohemorrhagic Escherichia coli
    Stefano Morabito
    Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanita, Rome, Italy
    Microb Drug Resist 8:85-91. 2002
    ..coli K-12 strain. In conclusion, integrons and antibiotic resistance genes can be frequently found in EHEC strains, particularly E. coli O111 and E. coli O26, and their presence could complicate therapeutic trials...
  39. pmc Evolution of IncA/C blaCMY-₂-carrying plasmids by acquisition of the blaNDM-₁ carbapenemase gene
    Alessandra Carattoli
    Department of Infectious, Parasitic, and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 56:783-6. 2012
    ..Considering that IncA/C plasmids possess a broad host range, this scaffold might support a large-scale diffusion of the bla(NDM-1) gene among Gram-negative rods...
  40. pmc Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approach
    Michela Flego
    Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Rome, Italy
    BMC Infect Dis 5:73. 2005
    ....
  41. ncbi request reprint Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis
    Daniela Fortini
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Clin Chem 53:1377-80. 2007
    ..Although the method has great differentiating power and reproducibility, faster genotyping at low cost may be requested to accurately identify B. anthracis strains in the field...
  42. doi request reprint Complete sequencing of an IncHI1 plasmid encoding the carbapenemase NDM-1, the ArmA 16S RNA methylase and a resistance-nodulation-cell division/multidrug efflux pump
    Monika Dolejska
    Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 68:34-9. 2013
    ..To characterize the pNDM-CIT plasmid identified in Citrobacter freundii carrying genes encoding the metallo-β-lactamase NDM-1 and the 16S RNA methylase ArmA...
  43. pmc Integrons and transposons on the Salmonella enterica serovar typhimurium virulence plasmid
    Laura Villa
    Department of Infectious, Parasitic, and Immuno mediated Disease, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Antimicrob Agents Chemother 49:1194-7. 2005
    ..A novel trimethoprim resistance gene, designated dfrA23, was also identified within the integron region. The association of multidrug resistance and virulence determinants represents an interesting example of virulence plasmid evolution...
  44. ncbi request reprint Molecular genotyping of Salmonella enterica Abortusovis by pulsed field gel electrophoresis
    Anna Maria Dionisi
    Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, I 00161 Rome, Italy
    Vet Microbiol 116:217-23. 2006
    ..In particular, this method could be used to identify the origin of infection during outbreaks within a single flock or in different herds...
  45. pmc Comparative analysis of IncHI2 plasmids carrying blaCTX-M-2 or blaCTX-M-9 from Escherichia coli and Salmonella enterica strains isolated from poultry and humans
    Aurora García Fernández
    Department of Infectious, Parasitic, Immune Mediated diseases, Instituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 51:4177-80. 2007
    ....
  46. pmc Deciphering the multifactorial nature of Acinetobacter baumannii pathogenicity
    Luísa C S Antunes
    Department of Biology, University Roma Tre, Rome, Italy
    PLoS ONE 6:e22674. 2011
    ..Many studies have focused on the epidemiology and antibiotic resistance of A. baumannii, but little is currently known with respect to its virulence potential...
  47. ncbi request reprint Composite integron array generated by insertion of an ORF341-type integron within a Tn21-like element
    Laura Villa
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Microb Drug Resist 8:1-8. 2002
    ..In-t1 insertion occurred within the 5'-conserved segment (5'-CS) proximal region of the resident In-t2...
  48. pmc Prediction of decreased susceptibility to penicillin of Neisseria meningitidis strains by real-time PCR
    Paola Stefanelli
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    J Clin Microbiol 41:4666-70. 2003
    ..This real-time PCR protocol characterizes the penicillin phenotype of N. meningitidis in a few hours without DNA sequencing and is useful for rapid screening of the penicillin-intermediate genotype among meningococcal isolates...
  49. doi request reprint Genomics of KPC-Producing Klebsiella pneumoniae Sequence Type 512 Clone Highlights the Role of RamR and Ribosomal S10 Protein Mutations in Conferring Tigecycline Resistance
    Laura Villa
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 58:1707-12. 2014
    ..A PCR-based approach was devised to amplify the potential resistance mechanisms identified by genomics and applied to two additional ST512 strains showing resistance to tigecycline, allowing us to identify mutations in the ramR gene. ..
  50. doi request reprint Reversion to susceptibility of a carbapenem-resistant clinical isolate of Klebsiella pneumoniae producing KPC-3
    Laura Villa
    Istituto Superiore di Sanita, Rome, Italy
    J Antimicrob Chemother 68:2482-6. 2013
    ..pneumoniae strain. Complete DNA sequences of the plasmids carried by the resistant and susceptible strains from this patient were determined...
  51. ncbi request reprint Use of 65 kDa mannoprotein gene primers in Real Time PCR identification of Candida albicans in biological samples
    Silvia Arancia
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161, Rome, Italy
    Mol Cell Probes 20:263-8. 2006
    ..Finally, the real-time PCR was employed in order to detect and quantify C. albicans in the sera from patients with invasive candidiasis...
  52. pmc Protocol for real-time PCR identification of anthrax spores from nasal swabs after broth enrichment
    Marco R Oggioni
    Dipartimento di Biologia Molecolare, Laboratorio di Microbiologia Molecolare e Biotecnologia, Universita di Siena, Siena, Rome, Italy
    J Clin Microbiol 40:3956-63. 2002
    ....
  53. pmc Variation in expression of HMW1 and HMW2 adhesins in invasive nontypeable Haemophilus influenzae isolates
    Maria Giufre
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    BMC Microbiol 8:83. 2008
    ..Number of 7-bp repeats within the hmwA promoters and presence of HMW proteins by Western blotting were also determined...
  54. ncbi request reprint Recombinant protein-based ELISA and immuno-cytochemical assay for the diagnosis of SARS
    Alessandra Carattoli
    Department of Infectious, Parasitic and Immuno Mediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    J Med Virol 76:137-42. 2005
    ..The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells...
  55. ncbi request reprint Functional identification of al-3 from Neurospora crassa as the gene for geranylgeranyl pyrophosphate synthase by complementation with crt genes, in vitro characterization of the gene product and mutant analysis
    G Sandmann
    Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universitat Konstanz, Germany
    J Photochem Photobiol B 18:245-51. 1993
    ..Cell-free studies with E. coli transformants gave direct evidence of the function of the al-3 protein as GGPP synthase and indicated that a short-chain prenylpyrophosphate, such as dimethylallyl pyrophosphate, is the genuine substrate...
  56. pmc Class 1 integron-borne multiple-antibiotic resistance carried by IncFI and IncL/M plasmids in Salmonella enterica serotype typhimurium
    F Tosini
    Laboratory of Cellular Biology, and Institute of Microbiology, University of Rome, La Sapienza, Rome, Italy
    Antimicrob Agents Chemother 42:3053-8. 1998
    ....
  57. pmc Multiple-antibiotic resistance mediated by structurally related IncL/M plasmids carrying an extended-spectrum beta-lactamase gene and a class 1 integron
    L Villa
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 44:2911-4. 2000
    ..3-kb bla(SHV-5)-In-t3 region of pSEM highlighted striking similarities with IncL/M plasmids isolated from nosocomial gram-negative pathogens, conferring resistance to expanded-spectrum cephalosporins and aminoglycosides...
  58. pmc Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998
    A Carattoli
    Laboratory of Bacteriology and Mycology, Istituto Superiore di Sanita, 00161 Rome, Italy
    Antimicrob Agents Chemother 46:1269-72. 2002
    ..These data suggest that the spread of cmy-2 among Salmonella strains is occurring through mobilization of the cmy-2 gene into different plasmid backbones and consequent horizontal transfer by conjugation...
  59. pmc CMY-13, a novel inducible cephalosporinase encoded by an Escherichia coli plasmid
    V Miriagou
    Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece
    Antimicrob Agents Chemother 48:3172-4. 2004
    ..ampC encoded a novel cephalosporinase (CMY-13) with activity similar to that of CMY-2. AmpR was likely functional as indicated in induction experiments...
  60. ncbi request reprint Comparison of multidrug resistance gene regions between two geographically unrelated Salmonella serotypes
    M Daly
    Molecular Diagnostics Unit, Cork Institute of Technology, Bishopstown, Cork, UK
    J Antimicrob Chemother 55:558-61. 2005
    ..serotype Typhimurium isolate, phage type DT193, isolated in Ireland and to compare them with resistance genes conferring plasmid-mediated multidrug resistance to a S. Enteritidis isolate from Italy...
  61. ncbi request reprint Sources of diversity of carbapenem resistance levels in Klebsiella pneumoniae carrying blaVIM-1
    A Loli
    Laboratory of Bacteriology, Institute Pasteur Hellenique, Athens, Greece
    J Antimicrob Chemother 58:669-72. 2006
    ..To elucidate the mechanisms responsible for the diversity of beta-lactam resistance phenotypes among isolates of a VIM-1-producing Klebsiella pneumoniae (VPKP) strain that is endemic in Greek hospitals...
  62. ncbi request reprint The Neurospora crassa carotenoid biosynthetic gene (albino 3) reveals highly conserved regions among prenyltransferases
    A Carattoli
    Dipartimento di Biopatologia Umana, Universita di Roma La Sapienza, Italy
    J Biol Chem 266:5854-9. 1991
    ..These homologous regions may be involved in the formation of the catalytic site of the prenyltransferases...
  63. pmc A chimeric light-regulated amino acid transport system allows the isolation of blue light regulator (blr) mutants of Neurospora crassa
    A Carattoli
    Dipartimento di Biopatologia Umana, Universita di Roma La Sapienza, Rome, Italy
    Proc Natl Acad Sci U S A 92:6612-6. 1995
    ..Mutations in the BLR strains are not allelic with white collar 1 or white collar 2, regulatory genes that are also involved in the response to blue light...
  64. ncbi request reprint Anthrax in animals and a farmer in Alto Adige, Italy
    P Kreidl
    Osservatorio Epidemiologico, Alto Adige, Italy
    Euro Surveill 11:E060216.3. 2006
  65. ncbi request reprint Prevalence of qnr genes among extended-spectrum beta-lactamase-producing enterobacterial isolates in Barcelona, Spain
    S LaVilla
    Department of Microbiology, Hospital Vall d Hebron, Universitat Autonoma de Barcelona, Barcelona, Spain
    J Antimicrob Chemother 61:291-5. 2008
    ..To evaluate the presence of qnr genes among enterobacterial isolates carrying extended-spectrum beta-lactamases (ESBLs) in Barcelona, Spain...