Research Topics
| G StantaSummaryAffiliation: International Centre for Genetic Engineering and Biotechnology Country: Italy Publications
| Collaborators
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Detail Information
Publications
Multicentre validation study of nucleic acids extraction from FFPE tissuesSerena Bonin
ACADEM Department University of Trieste and ICGEB, University of Trieste, Cattinara Hospital, 34149 Trieste, Italy
Virchows Arch 457:309-17. 2010..The results of the study emphasize the need for quality control of the nucleic acid extracts with standardised methods to prevent false negative results and to allow data comparison among different diagnostic laboratories...
The role of the pathologist in tissue banking: European Consensus Expert Group ReportGeneroso Bevilacqua
Division of Surgical, Molecular and Ultrastructural Pathology, University of Pisa and Pisa University Hospital, Medical School, Via Roma 57, 56126 Pisa, Italy
Virchows Arch 456:449-54. 2010....
The human RECQ1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferationRamiro Mendoza-Maldonado
International Centre for Genetic Engineering and Biotechnology Padriciano 99, 34149 Trieste, Italy
Mol Cancer 10:83. 2011..Current data show that the specific depletion of the human RECQ1 helicase leads to mitotic catastrophe in cancer cells and inhibition of tumor growth in mice...
Bioethical considerations on medical research using human tissues: the researcher's viewpointG Stanta
Dipartimento di Scienze Cliniche, , Trieste, Italy
Pathologica 100:67-75. 2008
A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissuesGiorgio Stanta
Department of Clinical, Morphological and Technological Sciences, University of Trieste, 34149 Trieste, Italy
Diagn Mol Pathol 15:115-23. 2006..Protein extracts from FineFix treated tissues were also compared with fresh tissues'ones by two dimensional electrophoresis, demonstrating that the protein pattern were well comparable for number and distribution of the spots...
DNA and RNA obtained from Bouin's fixed tissuesS Bonin
Department of Clinical, Morphological and Technological Sciences, University of Trieste, Cattinara Hospital, 34149 Trieste, Italy
J Clin Pathol 58:313-6. 2005..CONCLUSIONS: It is possible to recover and analyse DNA and RNA from Bouin's fixed and paraffin wax embedded tissues...
Catalytic subunit of telomerase expression is related to RNA component expressionG Stanta
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012, Trieste, Italy
FEBS Lett 460:285-8. 1999..Our results suggest that there may be a reciprocal control at the transcriptional level of the expression of hTRT and hTR which in turn is associated with tumor progression...
Prostate-tumor-inducing gene-1 analysis in human prostate cancer cells and tissue in relation to Mycoplasma infectionBruna Scaggiante
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, Italy
Cancer Invest 26:800-8. 2008..The overall data indicate that, in prostate tumor cell lines, PTI-1 presence parallels Mycoplasma infection suggesting that PTI-1 might not necessarily play a major role in the onset of prostate tumorigenesis...
[Old and new roots: paraffin goes from rags to riches]G Stanta
Dipartimento di Scienze Cliniche, Morfologiche e Tecnologiche, , International Centre for Genetic Engineering and Biotechnology, Trieste
Pathologica 95:276-7. 2003
Insulin-like growth factor system gene expression in women with type 2 diabetes and breast cancerE Nardon
Department of Clinical, Morphological and Technological Sciences, University of Trieste, Italy
J Clin Pathol 56:599-604. 2003..CONCLUSIONS: These differences in IGF-I/IGF-IR expression could contribute to the increased risk for breast cancer in women with type 2 diabetes...
Effects of formalin, methacarn, and fineFIX fixatives on RNA preservationIsabella Dotti
Dipartimento Universitario Clinico di Scienze Chirurgiche Generali, University of Trieste, Trieste, Italy
Diagn Mol Pathol 19:112-22. 2010..In fixed tissue samples, however, the potential effects of preanalytical tissue-related factors should be considered when performing mRNA quantitative analysis...
Higher random oligo concentration improves reverse transcription yield of cDNA from bioptic tissues and quantitative RT-PCR reliabilityErmanno Nardon
Department of Clinical, Morphological and Technological Sciences, University of Trieste, Italy
Exp Mol Pathol 87:146-51. 2009..Random priming at the 3.35 nmol/reaction concentration seems to be the most convenient strategy in assays using RNA obtained from FFPE tissues...
Causes of death and clinical diagnostic errors in extreme aged hospitalized people: a retrospective clinical-necropsy surveyP Bordin
Istituto di Clinica Medica, University of Trieste, Italy
J Gerontol A Biol Sci Med Sci 54:M554-9. 1999..There are little data on causes of death in extreme aged. We compared, using autopsy findings, main cause of death, overall disease status, and accuracy rate of clinical diagnoses in extreme aged and persons dying at younger ages...
Molecular analyses of sentinel lymph nodes: an open questionS Bonin
Department of Clinical, Morphological and Technological Sciences, University of Trieste, Ospedale di Cattinara, Italy
J Eur Acad Dermatol Venereol 16:34-9. 2002..Despite progress in the field with simultaneous detection of several markers it was assumed that tyrosinase mRNA remains the best target for the detection of metastatic melanoma cells...
Detection of spirochaetal DNA simultaneously in skin biopsies, peripheral blood and urine from patients with erythema migransPaolo Pauluzzi
Department of Clinical, Morphological and Technological Sciences, University of Trieste, Cattinara Hospital and ICGEB-International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
Acta Derm Venereol 84:106-10. 2004..These results demonstrate that Borrelia DNA is detectable systemically also in patients with early Lyme borreliosis and strongly suggest a possible dissemination of the causative agents even when only a local infection is assumed...
Evidence of multiple infectious agents in mycosis fungoides lesionsSerena Bonin
ACADEM Department, Unit of Dermatology, University of Trieste, Trieste, Italy
Exp Mol Pathol 89:46-50. 2010..However, these infectious agents do not seem to have effects on disease progression...
Evidence of CXC, CC and C chemokine production by lymphatic endothelial cellsSabrina Mancardi
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
Immunology 108:523-30. 2003....
Molecular characterisation of breast cancer patients at high and low recurrence riskSerena Bonin
Department of Clinical, Morphological and Technological Sciences, International Centre for Genetic Engineering and Biotechnology, University of Trieste, Trieste, Italy
Virchows Arch 452:241-50. 2008..The identification of these sub-groups of patients with higher risk of early relapse could have further involvement in the selection of the cases to submit to therapy against HER2 or CDK2 as a possible therapy target...
Expression of cyclin-dependent kinases and CDC25a phosphatase is related with recurrences and survival in women with peri- and post-menopausal breast cancerSerena Bonin
Department of Clinical, Morphological and Technological Sciences, University of Trieste, Trieste, and Surgical Pathology Unit, S Andrea Hospital, La Spezia, Italy
Virchows Arch 448:539-44. 2006..CDK2, CDK4 and CDC25a can be used as reliable biomarkers to predict prognosis in women with peri- and post-menopausal breast cancer...
Human discs large and scrib are localized at the same regions in colon mucosa and changes in their expression patterns are correlated with loss of tissue architecture during malignant progressionDaniela Gardiol
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
Int J Cancer 119:1285-90. 2006..The results, therefore, demonstrate that there is an inverse relationship between the levels of hDlg and hScrib expression and the loss of cell polarity and tissue architecture in the colon...
Proteomic studies on low- and high-grade human brain astrocytomasFederico Odreman
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy
J Proteome Res 4:698-708. 2005....
Bioethics considerations for medical research in human archive tissues: the point of view of the researcherGiorgio Stanta
Virchows Arch 453:117-9. 2008
Proteomic studies on the white matter of human brainStefano Pozzi Mucelli
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy
J Chromatogr B Analyt Technol Biomed Life Sci 833:80-90. 2006..This protein database obtained from the white matter of human brain contributes to deepen our knowledge on the molecular mechanisms that control several pathologies affecting this key component of the brain...
