Marco Guerrini

Summary

Affiliation: G. Ronzoni
Country: Italy

Publications

  1. ncbi request reprint Interaction of heparins with fibroblast growth factors: conformational aspects
    Marco Guerrini
    G Ronzoni, Institute for Chemical and Biochemical Research, Milan, Italy
    Curr Pharm Des 13:2045-56. 2007
  2. ncbi request reprint Low molecular weight heparins: structural differentiation by bidimensional nuclear magnetic resonance spectroscopy
    Marco Guerrini
    Institute for Chemical and Biochemical Research G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    Semin Thromb Hemost 33:478-87. 2007
  3. doi request reprint The tainted heparin story: an update
    Marco Guerrini
    Institute for Chemical and Biochemical Research, NMR Centre, Milan 20133, Italy
    Thromb Haemost 102:907-11. 2009
  4. doi request reprint An unusual antithrombin-binding heparin octasaccharide with an additional 3-O-sulfated glucosamine in the active pentasaccharide sequence
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    Biochem J 449:343-51. 2013
  5. pmc Conformational transitions induced in heparin octasaccharides by binding with antithrombin III
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    Biochem J 399:191-8. 2006
  6. ncbi request reprint Complex glycosaminoglycans: profiling substitution patterns by two-dimensional nuclear magnetic resonance spectroscopy
    Marco Guerrini
    Institute for Chemical and Biochemical Research G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    Anal Biochem 337:35-47. 2005
  7. pmc Antithrombin-binding octasaccharides and role of extensions of the active pentasaccharide sequence in the specificity and strength of interaction. Evidence for very high affinity induced by an unusual glucuronic acid residue
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    J Biol Chem 283:26662-75. 2008
  8. ncbi request reprint Undersulfated and glycol-split heparins endowed with antiangiogenic activity
    Benito Casu
    G Ronzoni Institute for Chemical and Biochemical Research, 20133 Milan, Italy
    J Med Chem 47:838-48. 2004
  9. doi request reprint Effects on molecular conformation and anticoagulant activities of 1,6-anhydrosugars at the reducing terminal of antithrombin-binding octasaccharides isolated from low-molecular-weight heparin enoxaparin
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    J Med Chem 53:8030-40. 2010
  10. pmc Heparin dodecasaccharide containing two antithrombin-binding pentasaccharides: structural features and biological properties
    Christian Viskov
    Istituto di Ricerche Chimiche e Biochimiche G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    J Biol Chem 288:25895-907. 2013

Collaborators

Detail Information

Publications28

  1. ncbi request reprint Interaction of heparins with fibroblast growth factors: conformational aspects
    Marco Guerrini
    G Ronzoni, Institute for Chemical and Biochemical Research, Milan, Italy
    Curr Pharm Des 13:2045-56. 2007
    ..Current NMR and molecular modelling studies are being extended to longer heparin oligosaccharides as well as to heparins with "glycol-split" residues along their chains...
  2. ncbi request reprint Low molecular weight heparins: structural differentiation by bidimensional nuclear magnetic resonance spectroscopy
    Marco Guerrini
    Institute for Chemical and Biochemical Research G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    Semin Thromb Hemost 33:478-87. 2007
    ..The use of a high-sensitivity NMR spectrometer (600 MHz equipped with cryoprobe) allowed the accurate quantification of residues with sensitivity better than 1 to 2%...
  3. doi request reprint The tainted heparin story: an update
    Marco Guerrini
    Institute for Chemical and Biochemical Research, NMR Centre, Milan 20133, Italy
    Thromb Haemost 102:907-11. 2009
    ..As such, these techniques are powerful tools for the analysis and assessment of heparin preparations...
  4. doi request reprint An unusual antithrombin-binding heparin octasaccharide with an additional 3-O-sulfated glucosamine in the active pentasaccharide sequence
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    Biochem J 449:343-51. 2013
    ..The presence of the second 3-O-sulfated glucosamine in the octasaccharide induced more than one order of magnitude increase in affinity to antithrombin compared to the pentasaccharide AGA*IA...
  5. pmc Conformational transitions induced in heparin octasaccharides by binding with antithrombin III
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    Biochem J 399:191-8. 2006
    ..Thus, for each AT binding sequence longer than AGA*IA, the interactions with the protein could differ and give to each heparin fragment a specific biological response...
  6. ncbi request reprint Complex glycosaminoglycans: profiling substitution patterns by two-dimensional nuclear magnetic resonance spectroscopy
    Marco Guerrini
    Institute for Chemical and Biochemical Research G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    Anal Biochem 337:35-47. 2005
    ..The method was applied to profile the substitution patterns of K5-PS derivatives involving different degrees of N,O-sulfation and N-acetylation, including O-sulfated heparosans bearing free amino groups...
  7. pmc Antithrombin-binding octasaccharides and role of extensions of the active pentasaccharide sequence in the specificity and strength of interaction. Evidence for very high affinity induced by an unusual glucuronic acid residue
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    J Biol Chem 283:26662-75. 2008
    ..Besides the well established ionic interactions, nonionic interactions may thus contribute to strengthen oligosaccharide-AT complexes...
  8. ncbi request reprint Undersulfated and glycol-split heparins endowed with antiangiogenic activity
    Benito Casu
    G Ronzoni Institute for Chemical and Biochemical Research, 20133 Milan, Italy
    J Med Chem 47:838-48. 2004
    ....
  9. doi request reprint Effects on molecular conformation and anticoagulant activities of 1,6-anhydrosugars at the reducing terminal of antithrombin-binding octasaccharides isolated from low-molecular-weight heparin enoxaparin
    Marco Guerrini
    G Ronzoni Institute for Chemical and Biochemical Research, Via G Colombo 81, 20133 Milan, Italy
    J Med Chem 53:8030-40. 2010
    ..The anti-FXa measured either in buffer or plasma milieu does not show any significant difference, suggesting that the inhibition of anti-FXa remains specific and biologically relevant...
  10. pmc Heparin dodecasaccharide containing two antithrombin-binding pentasaccharides: structural features and biological properties
    Christian Viskov
    Istituto di Ricerche Chimiche e Biochimiche G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    J Biol Chem 288:25895-907. 2013
    ..The dodecasaccharide displayed a significantly increased anti-FXa activity compared with those of the pentasaccharide, fondaparinux, and low molecular weight heparin enoxaparin...
  11. ncbi request reprint Active conformations of glycosaminoglycans. NMR determination of the conformation of heparin sequences complexed with antithrombin and fibroblast growth factors in solution
    Milos Hricovini
    Institute for Chemical and Biochemical Research G Ronzoni, Milan, Italy
    Semin Thromb Hemost 28:325-34. 2002
    ..These studies also confirm the crucial role of the 6-O-sulfate group on at least one glucosamine residue in the formation of the complex with FGF-1 but not with FGF-2...
  12. pmc Orthogonal analytical approaches to detect potential contaminants in heparin
    Marco Guerrini
    Istituto di Richerche Chimiche e Biochimiche G Ronzoni, via Giuseppe Colombo 81, 20133 Milan, Italy
    Proc Natl Acad Sci U S A 106:16956-61. 2009
    ..We show that given the complexity of heparin samples, and the requirement to ensure their purity and safety, use of orthogonal analytical techniques is effective at detecting an array of potential contaminants that could be present...
  13. ncbi request reprint Minimum FGF2 binding structural requirements of heparin and heparan sulfate oligosaccharides as determined by NMR spectroscopy
    Sara Guglier
    G Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy
    Biochemistry 47:13862-9. 2008
    ..Thus, our study highlights the fact that the conformational plurality of IdoA is able toaccommodate the changes in the sulfation pattern to provide the necessary specificity for protein binding...
  14. doi request reprint Structural features of low-molecular-weight heparins affecting their affinity to antithrombin
    Antonella Bisio
    Institute for Chemical and Biochemical Research, 20133 Milan, Italy
    Thromb Haemost 102:865-73. 2009
    ..This study is therefore critical for establishing correlations between structural features of LMWHs and their AT-mediated anticoagulant activity...
  15. ncbi request reprint Short heparin sequences spaced by glycol-split uronate residues are antagonists of fibroblast growth factor 2 and angiogenesis inhibitors
    Benito Casu
    G Ronzoni Institute for Chemical and Biochemical Research, 20133 Milan, Italy
    Biochemistry 41:10519-28. 2002
    ..Finally, it inhibited angiogenesis in a chick embrio chorioallantoic membrane (CAM) assay in which heparin is inactive...
  16. ncbi request reprint Minimal heparin/heparan sulfate sequences for binding to fibroblast growth factor-1
    Marco Guerrini
    Institute for Chemical and Biochemical Research G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    Biochem Biophys Res Commun 292:222-30. 2002
    ....
  17. ncbi request reprint A novel computational approach to integrate NMR spectroscopy and capillary electrophoresis for structure assignment of heparin and heparan sulfate oligosaccharides
    Marco Guerrini
    Institute for Chemical and Biochemical Research, G Ronzoni, Via G Colombo 81, 20133 Milan, Italy
    Glycobiology 12:713-9. 2002
    ..We demonstrate that the integration of NMR and CE data sets with the help of the PEN framework dramatically reduces the number of experimental constraints required to arrive at an HS GAG oligosaccharide sequence...
  18. pmc Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events
    Marco Guerrini
    Istituto di Ricerche Chimiche e Biochimiche G Ronzoni, Città Studi, via Giuseppe, Colombo 81, 20133 Milan, Italy
    Nat Biotechnol 26:669-75. 2008
    ..Our analysis suggests effective screening methods that can be used to determine whether or not heparin lots contain the contaminant reported here...
  19. ncbi request reprint Structural and conformational aspects of the anticoagulant and anti-thrombotic activity of heparin and dermatan sulfate
    Benito Casu
    Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy
    Curr Pharm Des 10:939-49. 2004
    ..Whereas it inactivates the binding site for AT causing a drop of the anticoagulant activity, it enhances the HCII-associated activity of both heparin and dermatan sulfate...
  20. pmc Structural features of glycol-split low-molecular-weight heparins and their heparin lyase generated fragments
    Anna Alekseeva
    Ronzoni Institute for Chemical and Biochemical Research, via Giuseppe Colombo 81, 20133, Milan, Italy
    Anal Bioanal Chem 406:249-65. 2014
    ..Typical LC-MS signatures of RO-derivatives of individual LMWH both before and after digestion with heparinases included oligosaccharides generated from the original antithrombin-binding and "linkage" regions. ..
  21. ncbi request reprint Influence of substitution pattern and cation binding on conformation and activity in heparin derivatives
    Timothy R Rudd
    School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, UK
    Glycobiology 17:983-93. 2007
    ..There are also obvious potential applications for these findings in the biotechnology sphere...
  22. ncbi request reprint Dynamic properties of biologically active synthetic heparin-like hexasaccharides
    Jesus Angulo
    Grupo de Carbohidratos, Instituto de Investigaciones Quimicas, CSIC, Americo Vespucio 49, Isla de la Cartuja, 41092 Sevilla, Spain
    Glycobiology 15:1008-15. 2005
    ..Chembiochem, 5, 55-61). Fast internal motions observed for the less sulphated compound 2, as compared with 1, may be related to their different behavior in stimulating FGF1-induced mitogenic activity...
  23. ncbi request reprint Identification and characterization of heparin/heparan sulfate binding domains of the endoglycosidase heparanase
    Flonia Levy-Adam
    Cancer and Vascular Biology Research Center, The Bruce Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel
    J Biol Chem 280:20457-66. 2005
    ..The two heparin/heparan sulfate recognition domains are potentially attractive targets for the development of heparanase inhibitors...
  24. ncbi request reprint Low-molecular-weight heparin and dermatan sulfate end group-labeled with tyramine and fluorescein. Biochemical and biological characterization of the fluorescent-labeled heparin derivative
    Job Harenberg
    1st Department of Medicine, Medical University Clinic, Mannheim, Germany
    Semin Thromb Hemost 28:343-54. 2002
    ..F-labeled heparin was displaced by unlabeled heparin from all three populations of leukocytes. Binding of heparin to leukocytes may play an important role in inflammation and atherosclerosis...
  25. ncbi request reprint Human milk oligosaccharides: an enzymatic protection step simplifies the synthesis of 3'- and 6'-O-sialyllactose and their analogues
    Anna Rencurosi
    Department of Organic and Industrial Chemistry, University of Milan, Via G Venezian, I 21 20133 Milan, Italy
    Carbohydr Res 337:473-83. 2002
    ..Moreover, the synthesis of the carboxymethyl and sulfo analogues of the title compounds is reported...
  26. ncbi request reprint Characterization of di- and monosulfated, unsaturated heparin disaccharides with terminal N-sulfated 1,6-anhydro-beta-D-glucosamine or N-sulfated 1,6-anhydro-beta-D-mannosamine residues
    Giuseppe Mascellani
    Opocrin S p A, Via Pacinotti 3, 41040 Corlo di Formigine, Modena, Italy
    Carbohydr Res 342:835-42. 2007
    ....
  27. ncbi request reprint Structure of a heteroxylan of gum exudate of the palm Scheelea phalerata (uricuri)
    Fernanda F Simas
    Departamento de Bioquimica, Universidade Federal do Parana, C P 19046, CEP 81531 990 Curitiba, PR, Brazil
    Phytochemistry 65:2347-55. 2004
    ..Alpha-GlcpA-(1 --> 2)-alphabeta-Xy1p and alpha-GlcpA-(1 --> 2)-beta-Xylp-(1 --> 4)-alphabeta-Xylp were obtained via partial acid hydrolysis of SPN, showing the structure of side-chain substituents on O-2 of the main-chain units...
  28. ncbi request reprint Cycloartane and oleanane saponins from egyptian astragalus spp. as modulators of lymphocyte proliferation
    Luisella Verotta
    Dipartimento di Chimica Organica e Industriale, Milano, Italy
    Planta Med 68:986-94. 2002
    ..endemic to Egypt were not cytotoxic against a variety of human cancer cells. However, dose-related modulation of lymphocyte proliferation was observed, and structure-activity relationships are described...