Christina De Castro

Summary

Affiliation: Federico II University
Country: Italy

Publications

  1. De Castro C, Molinaro A, Lanzetta R, Silipo A, Parrilli M. Lipopolysaccharide structures from Agrobacterium and Rhizobiaceae species. Carbohydr Res. 2008;343:1924-33 pubmed publisher
    ..This review reports and discusses the structural and the biological data available for the lipopolysaccharides from the Gram-negative bacterium Agrobacterium together with those of other related Rhizobiaceae species...
  2. De Castro C, Klose T, Speciale I, Lanzetta R, Molinaro A, Van Etten J, et al. Structure of the chlorovirus PBCV-1 major capsid glycoprotein determined by combining crystallographic and carbohydrate molecular modeling approaches. Proc Natl Acad Sci U S A. 2018;115:E44-E52 pubmed publisher
    ..Thus, combining X-ray crystallography with carbohydrate molecular modeling resulted in determining the complete glycosylated structure of a glycoprotein. In this case, it is the chlorovirus PBCV-1 major capsid protein. ..
  3. Speciale I, Duncan G, Unione L, Agarkova I, Garozzo D, Jimenez Barbero J, et al. The N-glycan structures of the antigenic variants of chlorovirus PBCV-1 major capsid protein help to identify the virus-encoded glycosyltransferases. J Biol Chem. 2019;294:5688-5699 pubmed publisher
    ..Our results uncover GT activities that assemble four of the nine residues of the PBCV-1 MCP N-glycans. ..
  4. De Castro C, Duncan G, Garozzo D, Molinaro A, Sturiale L, Tonetti M, et al. Biophysical Approaches to Solve the Structures of the Complex Glycan Shield of Chloroviruses. Adv Exp Med Biol. 2018;1104:237-257 pubmed publisher
    ..This chapter summarizes the methods used to solve the chlorovirus complex glycan structures with the hope that these methodologies can be used by scientists facing similar problems. ..
  5. Speciale I, Paciello I, Fazio L, Sturiale L, Palmigiano A, Lanzetta R, et al. Determination of the structure of the O-antigen and the lipid A from the entomopathogenic bacterium Pseudomonas entomophila lipopolysaccharide along with its immunological properties. Carbohydr Res. 2015;412:20-7 pubmed publisher
    ..The immunology experiments demonstrated that the LPS structure of P. entomophila displayed a low ability to engage the TLR4-mediated signaling correlated to a significant antagonistic activity toward hexa-acylated LPS structures. ..
  6. request reprint
    De Castro C, Carannante A, Lanzetta R, Liparoti V, Molinaro A, Parrilli M. Core oligosaccharide structure from the highly phytopathogenic Agrobacterium tumefaciens TT111 and conformational analysis of the putative rhamnan epitope. Glycobiology. 2006;16:1272-80 pubmed
    ..Its conformation was determined through extensive molecular dynamic (MD) analysis and presents an epitope similar to that recognized from the plant defense system. ..
  7. Kenyon J, Marzaioli A, Hall R, De Castro C. Structure of the K6 capsular polysaccharide from Acinetobacter baumannii isolate RBH4. Carbohydr Res. 2015;409:30-5 pubmed publisher
    ..The synthesis of these sugars could be attributed to genes in the KL6 capsule biosynthesis gene cluster, and the formation of the linkages between the sugars were assigned to glycosyltransferases or the Wzy polymerase encoded in KL6. ..
  8. Kenyon J, Hall R, De Castro C. Structural determination of the K14 capsular polysaccharide from an ST25 Acinetobacter baumannii isolate, D46. Carbohydr Res. 2015;417:52-6 pubmed publisher
    ..nosocomialis isolate LUH5541. The identity of the first sugar of the K14 repeat unit (K unit), and the functions of the four encoded glycosyltransferases and Wzy polymerase were predicted. ..
  9. De Castro C, Speciale I, Duncan G, Dunigan D, Agarkova I, Lanzetta R, et al. N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent. Angew Chem Int Ed Engl. 2016;55:654-8 pubmed publisher
    ..Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus. ..

More Information

Publications15

  1. Kenyon J, Duda K, De Felice A, Cunneen M, Molinaro A, Laitinen J, et al. Serotype O:8 isolates in the Yersinia pseudotuberculosis complex have different O-antigen gene clusters and produce various forms of rough LPS. Innate Immun. 2016;22:205-17 pubmed publisher
  2. Kenyon J, Speciale I, Hall R, De Castro C. Structure of repeating unit of the capsular polysaccharide from Acinetobacter baumannii D78 and assignment of the K4 gene cluster. Carbohydr Res. 2016;434:12-17 pubmed publisher
    ..The pyruvate acetal addition to galactosamine is formed by Ptr1, a novel pyruvate transferase, encoded at this locus. ..
  3. Van Etten J, Agarkova I, Dunigan D, Tonetti M, De Castro C, Duncan G. Chloroviruses Have a Sweet Tooth. Viruses. 2017;9: pubmed publisher
    ..This latter process differs from that of all other glycoprotein containing viruses that traditionally use the host endoplasmic reticulum and Golgi machinery to synthesize and transfer the glycans. ..
  4. request reprint
    De Castro C, Carannante A, Lanzetta R, Nunziata R, Piscopo V, Parrilli M. Elucidation of two O-chain structures from the lipopolysaccharide fraction of Agrobacterium tumefaciens F/1. Carbohydr Res. 2004;339:2451-5 pubmed
    ..Additional supporting data were obtained by periodate degradation, the major component was cleaved selectively, leading to a glucosamine glycoside, whereas the minor one was recovered unaffected...
  5. Kenyon J, Notaro A, Hsu L, De Castro C, Hall R. 5,7-Di-N-acetyl-8-epiacinetaminic acid: A new non-2-ulosonic acid found in the K73 capsule produced by an Acinetobacter baumannii isolate from Singapore. Sci Rep. 2017;7:11357 pubmed publisher
    ..This monosaccharide had not previously been detected in a biological sample but had been synthesized chemically. ..
  6. Kenyon J, Kasimova A, Notaro A, Arbatsky N, Speciale I, Shashkov A, et al. Acinetobacter baumannii K13 and K73 capsular polysaccharides differ only in K-unit side branches of novel non-2-ulosonic acids: di-N-acetylated forms of either acinetaminic acid or 8-epiacinetaminic acid. Carbohydr Res. 2017;452:149-155 pubmed publisher
    ..Glycosyltransferases predicted to catalyse the linkage of d-Galp or d-GalpNAc to l-FucpNAc in the growing K13 and K73 or K12 units, respectively, differ by only two amino acids. ..