G Scita

Summary

Affiliation: European Institute of Oncology
Country: Italy

Publications

  1. ncbi Signaling from Ras to Rac and beyond: not just a matter of GEFs
    G Scita
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti, 435, 20141 Milan, Italy
    EMBO J 19:2393-8. 2000
  2. ncbi Eps8 in the midst of GTPases
    Pier Paolo Di Fiore
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti, 435, 20141 Milan, Italy
    Int J Biochem Cell Biol 34:1178-83. 2002
  3. ncbi An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine
    G Scita
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    J Cell Biol 154:1031-44. 2001
  4. ncbi The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5
    L Lanzetti
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Nature 408:374-7. 2000
  5. ncbi EPS8 and E3B1 transduce signals from Ras to Rac
    G Scita
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Nature 401:290-3. 1999
  6. ncbi Eps8 controls actin-based motility by capping the barbed ends of actin filaments
    Andrea Disanza
    IFOM Istituto FIRC di Oncologia Molecolare Via Adamello 16, 20139, Milan, Italy
    Nat Cell Biol 6:1180-8. 2004
  7. ncbi Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells
    Silvia Goicoechea
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, NC, USA
    J Cell Sci 119:3316-24. 2006
  8. ncbi WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor
    Helene Bierne
    Unité des Interactions Bactéries Cellules, Institut Pasteur, INSERM U604, INRA USC2020, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Cell Sci 118:1537-47. 2005
  9. ncbi Abi1 is essential for the formation and activation of a WAVE2 signalling complex
    Metello Innocenti
    IFOM Istituto FIRC di Oncologia Molecolare Via Adamello 16, 20134, Milan, Italy
    Nat Cell Biol 6:319-27. 2004
  10. ncbi The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway
    Nina Offenhauser
    The FIRC Institute for Molecular Oncology, 20134 Milan, Italy
    Mol Biol Cell 15:91-8. 2004

Collaborators

  • Pier Paolo Di Fiore
  • Marie France Carlier
  • Arianna Tocchetti
  • James Casanova
  • Nina Offenhauser
  • Metello Innocenti
  • Andrea Disanza
  • Theresia E B Stradal
  • Emanuela Frittoli
  • Anika Steffen
  • Silke Gerboth
  • Klemens Rottner
  • Assunta Croce
  • Changsong Yang
  • Roy M Katso
  • Silvia Goicoechea
  • Firdous A Khanday
  • Maud Hertzog
  • Jing Shi
  • Andrea Palamidessi
  • Helene Bierne
  • Dominique Didry
  • Letizia Lanzetti
  • Jurgen Wehland
  • Stefano Confalonieri
  • L Lanzetti
  • Shin-ichiro Kojima
  • Lubov Czech
  • Tatyana Svitkina
  • Carol A Otey
  • Rafael Garcia-Mata
  • Hans-Juergen Kreienkamp
  • Olivier E Pardo
  • Jeremy Dericco
  • Marin Marinov
  • Daniel Arneman
  • Asma Naqvi
  • Alexandre Arcaro
  • Michael D Waterfield
  • Clemens M Franz
  • Artem Bugayenko
  • Angela De Laurentiis
  • Kaikobad Irani
  • Kerstin Berhoerster
  • Ilwola Mattagajasingh
  • Tohru Yamamori
  • Andrea Ciliberto
  • Sara Mantoani
  • Lakshmi Santhanam
  • Julian Downward
  • Francesca Milanesi
  • Kenji Kasuno
  • Hans Juergen Kreienkamp
  • Anne J Ridley
  • Stefanie Benesch
  • Frank B Gertler
  • Hiroaki Miki
  • Simona Polo
  • Frank P L Lai
  • Pascale Cossart
  • Tadaomi Takenawa
  • Carlo Tacchetti
  • Ralf Baumeister
  • Liliana B Areces
  • Maria Grazia Malabarba
  • Julia Ehinger
  • Liliana Areces
  • Adriana Zucconi
  • Giuseppe Cassata
  • Maria Cristina Gagliani
  • Saskia M Brachmann
  • Isabella Ponzanelli
  • John R Falck
  • Mario Faretta
  • Pierluigi Tenca
  • M Zerial
  • S Christoforidis
  • V Rybin
  • M G Malabarba

Detail Information

Publications25

  1. ncbi Signaling from Ras to Rac and beyond: not just a matter of GEFs
    G Scita
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti, 435, 20141 Milan, Italy
    EMBO J 19:2393-8. 2000
    ....
  2. ncbi Eps8 in the midst of GTPases
    Pier Paolo Di Fiore
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti, 435, 20141 Milan, Italy
    Int J Biochem Cell Biol 34:1178-83. 2002
    ..Thus, depending on its engagement in different complexes, Eps8 participates to EGFR signaling through Rac and endocytosis through Rab5...
  3. ncbi An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine
    G Scita
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    J Cell Biol 154:1031-44. 2001
    ..In this complex, determinants of Eps8 are responsible for the proper localization of the Rac-activating machine to sites of actin remodeling...
  4. ncbi The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5
    L Lanzetti
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Nature 408:374-7. 2000
    ..Thus, depending on its state of association with E3b1 or RN-tre, Eps8 participates in both EGFR signalling through Rac, and trafficking through Rab5...
  5. ncbi EPS8 and E3B1 transduce signals from Ras to Rac
    G Scita
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Nature 401:290-3. 1999
    ..We propose a model in which Eps8 mediates the transfer of signals between Ras and Rac, by forming a complex with E3b1 and Sos-1...
  6. ncbi Eps8 controls actin-based motility by capping the barbed ends of actin filaments
    Andrea Disanza
    IFOM Istituto FIRC di Oncologia Molecolare Via Adamello 16, 20139, Milan, Italy
    Nat Cell Biol 6:1180-8. 2004
    ....
  7. ncbi Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells
    Silvia Goicoechea
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, NC, USA
    J Cell Sci 119:3316-24. 2006
    ....
  8. ncbi WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor
    Helene Bierne
    Unité des Interactions Bactéries Cellules, Institut Pasteur, INSERM U604, INRA USC2020, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Cell Sci 118:1537-47. 2005
    ....
  9. ncbi Abi1 is essential for the formation and activation of a WAVE2 signalling complex
    Metello Innocenti
    IFOM Istituto FIRC di Oncologia Molecolare Via Adamello 16, 20134, Milan, Italy
    Nat Cell Biol 6:319-27. 2004
    ..Consistently, inhibition of Abi1 by RNA interference (RNAi) abrogates Rac-dependent lamellipodia protrusion. Thus, Abi1 orchestrates the proper assembly of the WAVE2 complex and mediates its activation at the leading edge in vivo...
  10. ncbi The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway
    Nina Offenhauser
    The FIRC Institute for Molecular Oncology, 20134 Milan, Italy
    Mol Biol Cell 15:91-8. 2004
    ..Finally, the patterns of expression of eps8 and eps8L2 in mice are remarkably overlapping, thus providing a likely explanation for the lack of overt phenotype in eps8 null mice...
  11. ncbi In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure
    Arianna Tocchetti
    Department of Medical Biochemistry, University of Goteborg, Sweden
    Genomics 81:234-44. 2003
    ..The data obtained will be instrumental in directing further experimental functional characterization of these genes...
  12. ncbi Phosphoinositide 3-kinase activates Rac by entering in a complex with Eps8, Abi1, and Sos-1
    Metello Innocenti
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
    J Cell Biol 160:17-23. 2003
    ..Our results define a mechanism whereby propagation of signals, originating from RTKs or Ras and leading to actin reorganization, is controlled by direct physical interaction between PI3K and a Rac-specific GEF complex...
  13. ncbi Mechanisms through which Sos-1 coordinates the activation of Ras and Rac
    Metello Innocenti
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti, 435, 20141 Milan, Italy
    J Cell Biol 156:125-36. 2002
    ..Thus, the involvement of Sos-1 at two distinct and differentially regulated steps of the signaling cascade allows for coordinated activation of Ras and Rac and different duration of their signaling within the cell...
  14. ncbi A novel actin barbed-end-capping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegans
    Assunta Croce
    IFOM Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20139 Milan, Italy
    Nat Cell Biol 6:1173-9. 2004
    ..Therefore, nematode genetics allowed not only the unmasking of distinct EPS-8-linked phenotypes, but also the definition of a novel function for this molecule in actin dynamics...
  15. ncbi Phosphoinositide 3-Kinase C2beta regulates cytoskeletal organization and cell migration via Rac-dependent mechanisms
    Roy M Katso
    Ludwig Institute for Cancer Research, Royal Free and University College Hospital Medical School, London W1W 7BS, United Kingdom
    Mol Biol Cell 17:3729-44. 2006
    ..Taken together, these findings suggest that PI3KC2beta regulates the migration and survival of human tumor cells by distinct molecular mechanisms...
  16. ncbi Increased ethanol resistance and consumption in Eps8 knockout mice correlates with altered actin dynamics
    Nina Offenhauser
    Fondazione Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20139 Milan, Italy
    Cell 127:213-26. 2006
    ..In addition, Eps8 null neurons are resistant to the actin-remodeling activities of NMDA and ethanol. We propose that proper regulation of the actin cytoskeleton is a key determinant of cellular and behavioral responses to ethanol...
  17. ncbi Regulation of cell shape by Cdc42 is mediated by the synergic actin-bundling activity of the Eps8-IRSp53 complex
    Andrea Disanza
    Istituto FIRC di Oncologia Molecolare IFOM, Via Adamello 16, 20139, Milan, Italy
    Nat Cell Biol 8:1337-47. 2006
    ..Collectively, these results support a model whereby the synergic bundling activity of the IRSp53-Eps8 complex, regulated by Cdc42, contributes to the generation of actin bundles, thus promoting filopodial protrusions...
  18. ncbi Sos-mediated activation of rac1 by p66shc
    Firdous A Khanday
    Cardiovascular Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    J Cell Biol 172:817-22. 2006
    ..This relationship between p66shc, grb2, and sos1 provides a novel mechanism for the activation of rac1...
  19. ncbi Protein complexes regulating Arp2/3-mediated actin assembly
    Theresia E B Stradal
    Signalling and Motility Group, German Research Centre for Biotechnology GBF, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Curr Opin Cell Biol 18:4-10. 2006
    ....
  20. ncbi Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes
    Metello Innocenti
    IFOM, Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20134, Milan, Italy
    Nat Cell Biol 7:969-76. 2005
    ..Thus, Abi1 is a dual regulator of WAVE and N-WASP activities in specific processes that are dependent on actin dynamics...
  21. ncbi WAVE2 signaling mediates invasion of polarized epithelial cells by Salmonella typhimurium
    Jing Shi
    Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, 22908-0732, USA
    J Biol Chem 280:29849-55. 2005
    ..Although infection leads to the formation of an IRSp53/WAVE2 complex, it is the association of WAVE2 with the Abi1/Nap1/PIR121/HSPC300 complex that regulates bacterial internalization...
  22. ncbi Regulation of actin dynamics by WASP and WAVE family proteins
    Theresia E B Stradal
    German Research Centre for Biotechnology, Department of Cell Biology, Mascheroder Weg 1, D-38124 Braunschweig, Germany
    Trends Cell Biol 14:303-11. 2004
  23. ncbi Rab5 is a signalling GTPase involved in actin remodelling by receptor tyrosine kinases
    Letizia Lanzetti
    Istituto Europeo di Oncologia, Via Ripamonti 435, 20141 Milan, Italy
    Nature 429:309-14. 2004
    ..This may aid crosslinking of actin fibres into actin networks at the plasma membrane. Thus, we have shown that Rab5 is a signalling GTPase and have elucidated the major molecular elements of its downstream pathway...
  24. ncbi Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation
    Anika Steffen
    German Research Centre for Biotechnology, Department of Cell Biology, Braunschweig, Germany
    EMBO J 23:749-59. 2004
    ..Thus, Sra-1 and Nap1 are constitutive and essential components of a WAVE2- and Abi-1-containing complex linking Rac to site-directed actin assembly...
  25. ncbi Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells
    Changsong Yang
    PLoS Biol 5:e317. 2007
    ..We propose that mDia2 plays an important role in formation of lamellipodia by nucleating and/or protecting from capping lamellipodial actin filaments, which subsequently exhibit high tendency to converge into filopodia...