Francesco Blasi

Summary

Affiliation: European Institute of Oncology
Country: Italy

Publications

  1. pmc Elevated urinary levels of urokinase-type plasminogen activator receptor (uPAR) in pancreatic ductal adenocarcinoma identify a clinically high-risk group
    Claudio Sorio
    Department of Pathology and Diagnostics, University of Verona, Italy
    BMC Cancer 11:448. 2011
  2. ncbi request reprint The urokinase receptor in hematopoietic stem cells mobilization
    Francesco Blasi
    San Raffaele Scientific Institute, Via Olgettina 60, Milano 20132 Italy
    Curr Pharm Des 17:1911-3. 2011
  3. doi request reprint The urokinase receptor: focused cell surface proteolysis, cell adhesion and signaling
    Francesco Blasi
    Universita Vita Salute San Raffaele, Milano, Italy
    FEBS Lett 584:1923-30. 2010
  4. ncbi request reprint Inhibition of HIV replication by the plasminogen activator is dependent on vitronectin-mediated cell adhesion
    Chiara Elia
    DIBIT, AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Via Olgettina, 58, 20132, Milan, Italy
    J Leukoc Biol 82:1212-20. 2007
  5. pmc Ligand-engaged urokinase-type plasminogen activator receptor and activation of the CD11b/CD18 integrin inhibit late events of HIV expression in monocytic cells
    Massimo Alfano
    AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Milan, Italy
    Blood 113:1699-709. 2009
  6. ncbi request reprint Domain 2 of the urokinase receptor contains an integrin-interacting epitope with intrinsic signaling activity: generation of a new integrin inhibitor
    Bernard Degryse
    Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
    J Biol Chem 280:24792-803. 2005
  7. ncbi request reprint The homeodomain transcription factor Prep1 (pKnox1) is required for hematopoietic stem and progenitor cell activity
    Patrizia Di Rosa
    Laboratory of Molecular Genetics, San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milano, Italy
    Dev Biol 311:324-34. 2007
  8. ncbi request reprint Specific immunofluorimetric assay detecting the chemotactic epitope of the urokinase receptor (uPAR)
    Massimo Resnati
    Laboratory of Molecular Genetics, DIBIT, H San Raffaele and Università Vita Salute San Raffaele, Via Olgettina 58, 20132, Milano, Italy
    J Immunol Methods 308:192-202. 2006
  9. pmc Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies
    Valeria R Caiolfa
    Department of Molecular Biology and Functional Genomics, Unit of Molecular Neuroscience, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Cell Biol 179:1067-82. 2007
  10. ncbi request reprint Expression of the urokinase plasminogen activator and its receptor in HIV-1-associated central nervous system disease
    Nicolai Sidenius
    Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    J Neuroimmunol 157:133-9. 2004

Collaborators

Detail Information

Publications32

  1. pmc Elevated urinary levels of urokinase-type plasminogen activator receptor (uPAR) in pancreatic ductal adenocarcinoma identify a clinically high-risk group
    Claudio Sorio
    Department of Pathology and Diagnostics, University of Verona, Italy
    BMC Cancer 11:448. 2011
    ..It is unknown whether the level of its soluble form (suPAR) in urine may be a diagnostic-prognostic marker in these patients...
  2. ncbi request reprint The urokinase receptor in hematopoietic stem cells mobilization
    Francesco Blasi
    San Raffaele Scientific Institute, Via Olgettina 60, Milano 20132 Italy
    Curr Pharm Des 17:1911-3. 2011
    ..Available data suggest that a similar mechanism may take place in humans...
  3. doi request reprint The urokinase receptor: focused cell surface proteolysis, cell adhesion and signaling
    Francesco Blasi
    Universita Vita Salute San Raffaele, Milano, Italy
    FEBS Lett 584:1923-30. 2010
    ..uPAR regulates the cells-extracellular matrix interactions promoting its degradation and turnover through the plasminogen activation cascade...
  4. ncbi request reprint Inhibition of HIV replication by the plasminogen activator is dependent on vitronectin-mediated cell adhesion
    Chiara Elia
    DIBIT, AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Via Olgettina, 58, 20132, Milan, Italy
    J Leukoc Biol 82:1212-20. 2007
    ..In conclusion, uPA inhibits HIV-1 replication in macrophages via up-regulation of cell adhesion to the substrate mediated by VN...
  5. pmc Ligand-engaged urokinase-type plasminogen activator receptor and activation of the CD11b/CD18 integrin inhibit late events of HIV expression in monocytic cells
    Massimo Alfano
    AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Milan, Italy
    Blood 113:1699-709. 2009
    ..Thus, either uPA/uPAR interaction, Mac-1 activation, or prevention of its association with uPAR triggers a signaling pathway leading to the inefficient release of HIV from monocytic cells...
  6. ncbi request reprint Domain 2 of the urokinase receptor contains an integrin-interacting epitope with intrinsic signaling activity: generation of a new integrin inhibitor
    Bernard Degryse
    Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
    J Biol Chem 280:24792-803. 2005
    ..This sequence thus plays a pivotal role in the regulation of uPAR-integrin interactions. Moreover, we describe a novel, very potent inhibitor of integrin-dependent cell migration...
  7. ncbi request reprint The homeodomain transcription factor Prep1 (pKnox1) is required for hematopoietic stem and progenitor cell activity
    Patrizia Di Rosa
    Laboratory of Molecular Genetics, San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milano, Italy
    Dev Biol 311:324-34. 2007
    ..1(+) (KSLA) cells and B-lymphocytes precursors agrees with the observed phenotype. We therefore conclude that Prep1 is required for a correct and complete hematopoiesis...
  8. ncbi request reprint Specific immunofluorimetric assay detecting the chemotactic epitope of the urokinase receptor (uPAR)
    Massimo Resnati
    Laboratory of Molecular Genetics, DIBIT, H San Raffaele and Università Vita Salute San Raffaele, Via Olgettina 58, 20132, Milano, Italy
    J Immunol Methods 308:192-202. 2006
    ..The immunofluorimetric assay will quantitate uPAR chemotactic fragments in biological samples, including serum and urine, and evaluate their diagnostic or prognostic potential in clinical studies...
  9. pmc Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies
    Valeria R Caiolfa
    Department of Molecular Biology and Functional Genomics, Unit of Molecular Neuroscience, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Cell Biol 179:1067-82. 2007
    ..These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents...
  10. ncbi request reprint Expression of the urokinase plasminogen activator and its receptor in HIV-1-associated central nervous system disease
    Nicolai Sidenius
    Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    J Neuroimmunol 157:133-9. 2004
    ..Additionally, these findings are consistent with a model in which overexpression of uPAR and overproduction of its soluble form may promote HIV replication via binding and removal of uPA from cell surface...
  11. pmc Down syndrome fibroblasts and mouse Prep1-overexpressing cells display increased sensitivity to genotoxic stress
    Nicola Micali
    Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S Raffaele Scientific Institute, Via Olgettina 60, Milan, Italy
    Nucleic Acids Res 38:3595-604. 2010
    ..Our results provide a rationale for the involvement of PREP1 in the apoptotic phenotype of DS tissues and indicate that differences in Prep1 level can have drastic effects...
  12. pmc Reduction of Prep1 levels affects differentiation of normal and malignant B cells and accelerates Myc driven lymphomagenesis
    Giorgio Iotti
    Laboratory of Transcriptional Regulation in Development and Cancer, Fondazione Istituto FIRC di Oncologia Molecolare, Milano, Italy
    PLoS ONE 7:e48353. 2012
    ..Therefore, the tumor-suppressive activity of Prep1 is intertwined with both the interference with Myc-induced apoptosis as well as with natural developmental functions of the protein...
  13. pmc Cytoplasmic Prep1 interacts with 4EHP inhibiting Hoxb4 translation
    J Carlos Villaescusa
    IFOM, FIRC Institute of Molecular Oncology, Milano, Italy
    PLoS ONE 4:e5213. 2009
    ..Interestingly, Prep1 contains a putative binding motif for 4EHP, which may reflect a novel unknown function...
  14. ncbi request reprint uPA binding increases UPAR localization to lipid rafts and modifies the receptor microdomain composition
    Macarena Sahores
    Molecular Genetics Unit, DIBIT, H S Raffaele, Department of Molecular Biology and Functional Genomics, Universita Vita Salute San Raffaele, Via Olgettina 60, 20132 Milan, Italy
    Biochim Biophys Acta 1778:250-9. 2008
    ..However, after treatment of cells with uPA or ATF the lipid environment is strongly impoverished of neutral glycosphingolipids...
  15. pmc Myb-binding protein 1A (MYBBP1A) is essential for early embryonic development, controls cell cycle and mitosis, and acts as a tumor suppressor
    Silvia Mori
    Universita Vita Salute San Raffaele, Milan, Italy
    PLoS ONE 7:e39723. 2012
    ..We conclude that MYBBP1A is an essential gene with novel roles at the pre-mitotic level and potential tumor suppressor activity...
  16. ncbi request reprint Pertussis toxin B-oligomer suppresses IL-6 induced HIV-1 and chemokine expression in chronically infected U1 cells via inhibition of activator protein 1
    Chiara Rizzi
    AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Milan, Italy
    J Immunol 176:999-1006. 2006
    ..Thus, PTX-B suppression of IL-6 induced expression of HIV and cellular genes in chronically infected promonocytic cells is strongly correlated to inhibition of AP-1...
  17. pmc Involvement of Prep1 in the alphabeta T-cell receptor T-lymphocytic potential of hematopoietic precursors
    Dmitri Penkov
    Molecular Genetics Unit, Universita Vita Salute San Raffaele, Milan, Italy
    Mol Cell Biol 25:10768-81. 2005
    ..Therefore, the deficient postnatal T-lymphocytic potential of the Prep1 hematopoietic progenitors depends on the combined, not compensated, absence of Prep1 and at least Pbx2...
  18. ncbi request reprint The urokinase receptor is overexpressed in the AIDS dementia complex and other neurological manifestations
    Paola Cinque
    Clinic of Infectious Diseases, San Raffaele Scientific Institute, University of Milan, Italy
    Ann Neurol 55:687-94. 2004
    ..The overexpression of uPAR in the CNS of patients with ADC suggests that the uPA/uPAR system may contribute to the tissue injury and neuronal damage in this disease...
  19. ncbi request reprint The soluble D2D3(88-274) fragment of the urokinase receptor inhibits monocyte chemotaxis and integrin-dependent cell adhesion
    Federico Furlan
    Department of Molecular Biology and Functional Genomics, San Raffaele Scientific Institute, Milan, 20132, Italy
    J Cell Sci 117:2909-16. 2004
    ....
  20. pmc Urokinase-urokinase receptor interaction mediates an inhibitory signal for HIV-1 replication
    Massimo Alfano
    AIDS Immunopathogenesis Unit, Department of Immunology and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina n 58, 20132 Milan, Italy
    Proc Natl Acad Sci U S A 99:8862-7. 2002
    ....
  21. ncbi request reprint Metalloproteases cleave the urokinase-type plasminogen activator receptor in the D1-D2 linker region and expose epitopes not present in the intact soluble receptor
    Annapaola Andolfo
    Department of Molecular Pathology and Medicine, Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Milan, Italy
    Thromb Haemost 88:298-306. 2002
    ..Using an antibody raised against the human uPAR linker region we show that this region of uPAR, which contains the chemotactic SRSRY epitope, is exposed upon MMP cleavage...
  22. doi request reprint uPAR-deficient mouse keratinocytes fail to produce EGFR-dependent laminin-5, affecting migration in vivo and in vitro
    Silvia D'Alessio
    Università Vita Salute San Raffaele and Istituto Scientifico H San Raffaele, Via Olgettina 60, 20132 Milano, Italy
    J Cell Sci 121:3922-32. 2008
    ..These results were confirmed in vivo, with LN5 being upregulated during wound healing in wild-type but not in uPAR-KO epidermis...
  23. ncbi request reprint The urokinase plasminogen activator system in cancer: recent advances and implication for prognosis and therapy
    Nicolai Sidenius
    Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
    Cancer Metastasis Rev 22:205-22. 2003
    ..This review is focused on recent insight into the cancer related biology of the uPA/uPAR system as well as its implications for clinical cancer diagnosis, prognosis and therapy...
  24. pmc Hoxb1 enhancer and control of rhombomere 4 expression: complex interplay between PREP1-PBX1-HOXB1 binding sites
    Elisabetta Ferretti
    Molecular Genetics Unit, Department of Molecular Biology and Functional Genomics, Istituto Scientifico H San Raffaele, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
    Mol Cell Biol 25:8541-52. 2005
    ....
  25. pmc Prep1 directly regulates the intrinsic apoptotic pathway by controlling Bcl-XL levels
    Nicola Micali
    Laboratory of Molecular Genetics, DIBIT, S Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy
    Mol Cell Biol 29:1143-51. 2009
    ..Thus, Prep1 directly controls mitochondrial homeostasis (and the apoptotic potential) by modulating Bcl-x gene expression...
  26. ncbi request reprint The urokinase receptor and the regulation of cell proliferation
    Roberta Mazzieri
    Universita Vita Salute San Raffaele, Via Olgettina 60, 20132 Milan, Italy
    Thromb Haemost 93:641-6. 2005
    ..Alteration of such a complex and finely modulated mechanism results in unregulated cell proliferation and altered tissue organization, typically associated with tumor progression...
  27. pmc Dimerization controls the lipid raft partitioning of uPAR/CD87 and regulates its biological functions
    Orla Cunningham
    Molecular Genetics Unit, Department of Molecular Biology and Functional Genomics, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
    EMBO J 22:5994-6003. 2003
    ..In contrast to uPA, the binding of Vn occurred preferentially to raft- associated dimeric uPAR and was completely blocked by cholesterol depletion...
  28. ncbi request reprint uPAR: a versatile signalling orchestrator
    Francesco Blasi
    Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milano, Italy
    Nat Rev Mol Cell Biol 3:932-43. 2002
    ....
  29. ncbi request reprint The urokinase receptor as an entertainer of signal transduction
    Silvia D'Alessio
    Department of Molecular Biology and Functional Genomics, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
    Front Biosci (Landmark Ed) 14:4575-87. 2009
    ..This review is focused on the biochemistry of the pathways affected by uPAR and its partners...
  30. ncbi request reprint uPA and uPAR in fibrinolysis, immunity and pathology
    Anna Mondino
    Department of Immunology and Infectious Diseases, Cancer Immunotherapy and Gene Therapy Program, San Raffaele Scientific Institute, DIBIT, Via Olgettina 58, 20132 Milan, Italy
    Trends Immunol 25:450-5. 2004
  31. pmc Proteomics analysis of nucleolar SUMO-1 target proteins upon proteasome inhibition
    Vittoria Matafora
    Division of Genomics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy
    Mol Cell Proteomics 8:2243-55. 2009
    ..The analysis of the nature of the SUMO-1 targets identified in this study strongly indicates that sumoylation, acting in coordination with the ubiquitin-proteasome system, regulates the maintenance of nucleolar integrity...
  32. ncbi request reprint Urokinase regulates vitronectin binding by controlling urokinase receptor oligomerization
    Nicolai Sidenius
    Molecular Genetics Unit, DIBIT, Universita Vita Salute San Raffaele, 20132 Milan, Italy
    J Biol Chem 277:27982-90. 2002
    ....