Research Topics
Genomes and Genes | Francesco BlasiSummaryAffiliation: European Institute of Oncology Country: Italy Publications
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Detail Information
Publications
Elevated urinary levels of urokinase-type plasminogen activator receptor (uPAR) in pancreatic ductal adenocarcinoma identify a clinically high-risk groupClaudio Sorio
Department of Pathology and Diagnostics, University of Verona, Italy
BMC Cancer 11:448. 2011..It is unknown whether the level of its soluble form (suPAR) in urine may be a diagnostic-prognostic marker in these patients...
The urokinase receptor: focused cell surface proteolysis, cell adhesion and signalingFrancesco Blasi
Universita Vita Salute San Raffaele, Milano, Italy
FEBS Lett 584:1923-30. 2010..uPAR regulates the cells-extracellular matrix interactions promoting its degradation and turnover through the plasminogen activation cascade...
The urokinase receptor in hematopoietic stem cells mobilizationFrancesco Blasi
San Raffaele Scientific Institute, Via Olgettina 60, Milano 20132 Italy
Curr Pharm Des 17:1911-3. 2011..Available data suggest that a similar mechanism may take place in humans...- Inhibition of HIV replication by the plasminogen activator is dependent on vitronectin-mediated cell adhesionChiara Elia
DIBIT, AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Via Olgettina, 58, 20132, Milan, Italy
J Leukoc Biol 82:1212-20. 2007..In conclusion, uPA inhibits HIV-1 replication in macrophages via up-regulation of cell adhesion to the substrate mediated by VN... - Ligand-engaged urokinase-type plasminogen activator receptor and activation of the CD11b/CD18 integrin inhibit late events of HIV expression in monocytic cellsMassimo Alfano
AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Milan, Italy
Blood 113:1699-709. 2009..Thus, either uPA/uPAR interaction, Mac-1 activation, or prevention of its association with uPAR triggers a signaling pathway leading to the inefficient release of HIV from monocytic cells... - Domain 2 of the urokinase receptor contains an integrin-interacting epitope with intrinsic signaling activity: generation of a new integrin inhibitorBernard Degryse
Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
J Biol Chem 280:24792-803. 2005..This sequence thus plays a pivotal role in the regulation of uPAR-integrin interactions. Moreover, we describe a novel, very potent inhibitor of integrin-dependent cell migration... - Specific immunofluorimetric assay detecting the chemotactic epitope of the urokinase receptor (uPAR)Massimo Resnati
Laboratory of Molecular Genetics, DIBIT, , Via Olgettina 58, 20132, Milano, Italy
J Immunol Methods 308:192-202. 2006..The immunofluorimetric assay will quantitate uPAR chemotactic fragments in biological samples, including serum and urine, and evaluate their diagnostic or prognostic potential in clinical studies... - The homeodomain transcription factor Prep1 (pKnox1) is required for hematopoietic stem and progenitor cell activityPatrizia Di Rosa
Laboratory of Molecular Genetics, San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milano, Italy
Dev Biol 311:324-34. 2007..1(+) (KSLA) cells and B-lymphocytes precursors agrees with the observed phenotype. We therefore conclude that Prep1 is required for a correct and complete hematopoiesis... - Expression of the urokinase plasminogen activator and its receptor in HIV-1-associated central nervous system diseaseNicolai Sidenius
Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
J Neuroimmunol 157:133-9. 2004..Additionally, these findings are consistent with a model in which overexpression of uPAR and overproduction of its soluble form may promote HIV replication via binding and removal of uPA from cell surface... - Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assembliesValeria R Caiolfa
Department of Molecular Biology and Functional Genomics, Unit of Molecular Neuroscience, San Raffaele Scientific Institute, 20132 Milano, Italy
J Cell Biol 179:1067-82. 2007..These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents... - Down syndrome fibroblasts and mouse Prep1-overexpressing cells display increased sensitivity to genotoxic stressNicola Micali
Laboratory of Molecular Dynamics of the Nucleus, Division of Genetics and Cell Biology, S Raffaele Scientific Institute, Via Olgettina 60, Milan, Italy
Nucleic Acids Res 38:3595-604. 2010..Our results provide a rationale for the involvement of PREP1 in the apoptotic phenotype of DS tissues and indicate that differences in Prep1 level can have drastic effects... - Cytoplasmic Prep1 interacts with 4EHP inhibiting Hoxb4 translationJ Carlos Villaescusa
IFOM, FIRC Institute of Molecular Oncology, Milano, Italy
PLoS ONE 4:e5213. 2009..Interestingly, Prep1 contains a putative binding motif for 4EHP, which may reflect a novel unknown function... - Myb-binding protein 1A (MYBBP1A) is essential for early embryonic development, controls cell cycle and mitosis, and acts as a tumor suppressorSilvia Mori
Universita Vita Salute San Raffaele, Milan, Italy
PLoS ONE 7:e39723. 2012..We conclude that MYBBP1A is an essential gene with novel roles at the pre-mitotic level and potential tumor suppressor activity... - Pertussis toxin B-oligomer suppresses IL-6 induced HIV-1 and chemokine expression in chronically infected U1 cells via inhibition of activator protein 1Chiara Rizzi
AIDS Immunopathogenesis Unit, San Raffaele Scientific Institute, Milan, Italy
J Immunol 176:999-1006. 2006..Thus, PTX-B suppression of IL-6 induced expression of HIV and cellular genes in chronically infected promonocytic cells is strongly correlated to inhibition of AP-1... - Involvement of Prep1 in the alphabeta T-cell receptor T-lymphocytic potential of hematopoietic precursorsDmitri Penkov
Molecular Genetics Unit, Universita Vita Salute San Raffaele, Milan, Italy
Mol Cell Biol 25:10768-81. 2005..Therefore, the deficient postnatal T-lymphocytic potential of the Prep1 hematopoietic progenitors depends on the combined, not compensated, absence of Prep1 and at least Pbx2... - uPAR-deficient mouse keratinocytes fail to produce EGFR-dependent laminin-5, affecting migration in vivo and in vitroSilvia D'Alessio
Università Vita Salute San Raffaele and Istituto Scientifico H San Raffaele, Via Olgettina 60, 20132 Milano, Italy
J Cell Sci 121:3922-32. 2008..These results were confirmed in vivo, with LN5 being upregulated during wound healing in wild-type but not in uPAR-KO epidermis... - Urokinase-urokinase receptor interaction mediates an inhibitory signal for HIV-1 replicationMassimo Alfano
AIDS Immunopathogenesis Unit, Department of Immunology and Infectious Diseases, San Raffaele Scientific Institute, Via Olgettina n 58, 20132 Milan, Italy
Proc Natl Acad Sci U S A 99:8862-7. 2002.... - uPA binding increases UPAR localization to lipid rafts and modifies the receptor microdomain compositionMacarena Sahores
Molecular Genetics Unit, DIBIT, H S Raffaele, Department of Molecular Biology and Functional Genomics, Universita Vita Salute San Raffaele, Via Olgettina 60, 20132 Milan, Italy
Biochim Biophys Acta 1778:250-9. 2008..However, after treatment of cells with uPA or ATF the lipid environment is strongly impoverished of neutral glycosphingolipids... - Metalloproteases cleave the urokinase-type plasminogen activator receptor in the D1-D2 linker region and expose epitopes not present in the intact soluble receptorAnnapaola Andolfo
Department of Molecular Pathology and Medicine, Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Milan, Italy
Thromb Haemost 88:298-306. 2002..Using an antibody raised against the human uPAR linker region we show that this region of uPAR, which contains the chemotactic SRSRY epitope, is exposed upon MMP cleavage... - The soluble D2D3(88-274) fragment of the urokinase receptor inhibits monocyte chemotaxis and integrin-dependent cell adhesionFederico Furlan
Department of Molecular Biology and Functional Genomics, San Raffaele Scientific Institute, Milan, 20132, Italy
J Cell Sci 117:2909-16. 2004.... - Hoxb1 enhancer and control of rhombomere 4 expression: complex interplay between PREP1-PBX1-HOXB1 binding sitesElisabetta Ferretti
Molecular Genetics Unit, Department of Molecular Biology and Functional Genomics, Istituto Scientifico H San Raffaele, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
Mol Cell Biol 25:8541-52. 2005.... - Prep1 directly regulates the intrinsic apoptotic pathway by controlling Bcl-XL levelsNicola Micali
Laboratory of Molecular Genetics, DIBIT, S Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy
Mol Cell Biol 29:1143-51. 2009..Thus, Prep1 directly controls mitochondrial homeostasis (and the apoptotic potential) by modulating Bcl-x gene expression... - The urokinase receptor and the regulation of cell proliferationRoberta Mazzieri
Universita Vita Salute San Raffaele, Via Olgettina 60, 20132 Milan, Italy
Thromb Haemost 93:641-6. 2005..Alteration of such a complex and finely modulated mechanism results in unregulated cell proliferation and altered tissue organization, typically associated with tumor progression... - The urokinase receptor is overexpressed in the AIDS dementia complex and other neurological manifestationsPaola Cinque
Clinic of Infectious Diseases, San Raffaele Scientific Institute, University of Milan, Italy
Ann Neurol 55:687-94. 2004..The overexpression of uPAR in the CNS of patients with ADC suggests that the uPA/uPAR system may contribute to the tissue injury and neuronal damage in this disease... - The urokinase plasminogen activator system in cancer: recent advances and implication for prognosis and therapyNicolai Sidenius
Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milan, Italy
Cancer Metastasis Rev 22:205-22. 2003..This review is focused on recent insight into the cancer related biology of the uPA/uPAR system as well as its implications for clinical cancer diagnosis, prognosis and therapy... - uPAR: a versatile signalling orchestratorFrancesco Blasi
Department of Molecular Biology and Functional Genomics, DIBIT, Universita Vita Salute San Raffaele, Via Olgettina 58, 20132 Milano, Italy
Nat Rev Mol Cell Biol 3:932-43. 2002.... - Reduction of Prep1 levels affects differentiation of normal and malignant B cells and accelerates Myc driven lymphomagenesisGiorgio Iotti
Laboratory of Transcriptional Regulation in Development and Cancer, Fondazione Istituto FIRC di Oncologia Molecolare, Milano, Italy
PLoS ONE 7:e48353. 2012..Therefore, the tumor-suppressive activity of Prep1 is intertwined with both the interference with Myc-induced apoptosis as well as with natural developmental functions of the protein... - The urokinase receptor as an entertainer of signal transductionSilvia D'Alessio
Department of Molecular Biology and Functional Genomics, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
Front Biosci 14:4575-87. 2009..This review is focused on the biochemistry of the pathways affected by uPAR and its partners... - uPA and uPAR in fibrinolysis, immunity and pathologyAnna Mondino
Department of Immunology and Infectious Diseases, Cancer Immunotherapy and Gene Therapy Program, San Raffaele Scientific Institute, DIBIT, Via Olgettina 58, 20132 Milan, Italy
Trends Immunol 25:450-5. 2004 - Proteomics analysis of nucleolar SUMO-1 target proteins upon proteasome inhibitionVittoria Matafora
Division of Genomics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy
Mol Cell Proteomics 8:2243-55. 2009..The analysis of the nature of the SUMO-1 targets identified in this study strongly indicates that sumoylation, acting in coordination with the ubiquitin-proteasome system, regulates the maintenance of nucleolar integrity... - Urokinase regulates vitronectin binding by controlling urokinase receptor oligomerizationNicolai Sidenius
Molecular Genetics Unit, DIBIT, Universita Vita Salute San Raffaele, 20132 Milan, Italy
J Biol Chem 277:27982-90. 2002....