Raffaele Lombardi

Summary

Affiliation: ENEA CR Casaccia
Country: Italy

Publications

  1. doi request reprint Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation
    Raffaele Lombardi
    ENEA, Laboratorio Biotecnologie, UTBIORAD, C R Casaccia, Via Anguillarese 301, 00123, Rome, Italy
    Transgenic Res 21:1005-21. 2012
  2. pmc High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus
    Raffaele Lombardi
    Centro Ricerche Casaccia, Via Anguillarese 301, I 00123, Rome, Italy
    BMC Biotechnol 9:96. 2009
  3. doi request reprint Optimisation of the purification process of a tumour-targeting antibody produced in N. benthamiana using vacuum-agroinfiltration
    Raffaele Lombardi
    ENEA, Dipartimento BIOTEC, Sezione Genetica e Genomica Vegetale, C R Casaccia, Via Anguillarese 301, 00123, Rome, Italy
    Transgenic Res 19:1083-97. 2010
  4. doi request reprint Plant pharming of a full-sized, tumour-targeting antibody using different expression strategies
    Maria Elena Villani
    ENEA, Dipartimento BAS, Sezione Genetica e Genomica Vegetale, C R Casaccia, Via Anguillarese 301, I 00123, Rome, Italy
    Plant Biotechnol J 7:59-72. 2009
  5. doi request reprint Proteomic analysis of the plant-virus interaction in cucumber mosaic virus (CMV) resistant transgenic tomato
    Mariasole Di Carli
    ENEA, Centro Ricerche Casaccia, Via Anguillarese 301, I 00123, Rome, Italy
    J Proteome Res 9:5684-97. 2010
  6. pmc Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells
    Carla Marusic
    ENEA BIOTEC Sezione Genetica e Genomica Vegetale, C R Casaccia, 00060 Rome, Italy
    BMC Biotechnol 7:12. 2007

Collaborators

Detail Information

Publications6

  1. doi request reprint Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation
    Raffaele Lombardi
    ENEA, Laboratorio Biotecnologie, UTBIORAD, C R Casaccia, Via Anguillarese 301, 00123, Rome, Italy
    Transgenic Res 21:1005-21. 2012
    ..Identification of possible 'fragile' sites in the H10 antibody hinge region could be of general interest for the development of new strategies to reduce antibody degradation and increase the yield of intact IgGs in plants...
  2. pmc High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus
    Raffaele Lombardi
    Centro Ricerche Casaccia, Via Anguillarese 301, I 00123, Rome, Italy
    BMC Biotechnol 9:96. 2009
    ..In this work we describe a transient expression system based on co-agroinfiltration of plant virus gene silencing suppressor proteins in Nicotiana benthamiana, followed by a two-step affinity purification protocol of plant-derived Nef...
  3. doi request reprint Optimisation of the purification process of a tumour-targeting antibody produced in N. benthamiana using vacuum-agroinfiltration
    Raffaele Lombardi
    ENEA, Dipartimento BIOTEC, Sezione Genetica e Genomica Vegetale, C R Casaccia, Via Anguillarese 301, 00123, Rome, Italy
    Transgenic Res 19:1083-97. 2010
    ..4% purity, free of phenolic and alkaloid compounds with low endotoxin levels (<1 EU/ml)...
  4. doi request reprint Plant pharming of a full-sized, tumour-targeting antibody using different expression strategies
    Maria Elena Villani
    ENEA, Dipartimento BAS, Sezione Genetica e Genomica Vegetale, C R Casaccia, Via Anguillarese 301, I 00123, Rome, Italy
    Plant Biotechnol J 7:59-72. 2009
    ....
  5. doi request reprint Proteomic analysis of the plant-virus interaction in cucumber mosaic virus (CMV) resistant transgenic tomato
    Mariasole Di Carli
    ENEA, Centro Ricerche Casaccia, Via Anguillarese 301, I 00123, Rome, Italy
    J Proteome Res 9:5684-97. 2010
    ....
  6. pmc Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells
    Carla Marusic
    ENEA BIOTEC Sezione Genetica e Genomica Vegetale, C R Casaccia, 00060 Rome, Italy
    BMC Biotechnol 7:12. 2007
    ..Two isoforms of Nef protein can be found in cells: a full-length N-terminal myristoylated form (p27, 27 kDa) and a truncated form (p25, 25 kDa). Here we report the expression and purification of HIV Nef from transgenic tobacco...