Giovanni Delogu

Summary

Affiliation: Catholic University
Country: Italy

Publications

  1. ncbi request reprint The quest for a new vaccine against tuberculosis
    Giovanni Delogu
    Institute of Microbiology, Catholic University of the Sacred Hearth, Largo A Gemelli, Rome, Italy
    J Infect Dev Ctries 3:5-15. 2009
  2. ncbi request reprint The spectrum of tuberculosis infection: new perspectives in the era of biologics
    Giovanni Delogu
    Address correspondence to Prof Delogu, Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Largo A Gemelli, 8 00168 Rome, Italy E mail
    J Rheumatol Suppl 91:11-6. 2014
  3. ncbi request reprint Impact of structural domains of the heparin binding hemagglutinin of Mycobacterium tuberculosis on function
    Giovanni Delogu
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Rome, Italy
    Protein Pept Lett 19:1035-9. 2012
  4. ncbi request reprint Microbiological and immunological diagnosis of tuberculous spondylodiscitis
    G Delogu
    Institute of Microbiology, School of Medicine, Catholic University of the Sacred Heart, Rome, Italy
    Eur Rev Med Pharmacol Sci 16:73-8. 2012
  5. ncbi request reprint PE_PGRS proteins are differentially expressed by Mycobacterium tuberculosis in host tissues
    Giovanni Delogu
    Institute of Microbiology, Catholic University of Sacred Heart, L go A Gemelli, 8, 00168 Rome, Italy
    Microbes Infect 8:2061-7. 2006
  6. ncbi request reprint Rv1818c-encoded PE_PGRS protein of Mycobacterium tuberculosis is surface exposed and influences bacterial cell structure
    Giovanni Delogu
    Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43 B, 07100 Sassari, Italy
    Mol Microbiol 52:725-33. 2004
  7. ncbi request reprint Expression and purification of recombinant methylated HBHA in Mycobacterium smegmatis
    Giovanni Delogu
    Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43 B, 07100 Sassari
    FEMS Microbiol Lett 239:33-9. 2004
  8. pmc The hbhA gene of Mycobacterium tuberculosis is specifically upregulated in the lungs but not in the spleens of aerogenically infected mice
    Giovanni Delogu
    Institute of Microbiology, Catholic University of the Sacred Hearth, Largo Gemelli 8, 00168 Rome, Italy
    Infect Immun 74:3006-11. 2006
  9. ncbi request reprint Heparin binding haemagglutin as potential diagnostic marker of Mycobacterium bovis
    Paola Molicotti
    Department of Biomedical Sciences, University of Sassari, Italy
    New Microbiol 31:423-7. 2008
  10. ncbi request reprint Functional dissection of protein domains involved in the immunomodulatory properties of PE_PGRS33 of Mycobacterium tuberculosis
    Antonella Zumbo
    Institute of Microbiology, Universita Cattolica del Sacro Cuore, Rome, Italy
    Pathog Dis 69:232-9. 2013

Collaborators

Detail Information

Publications49

  1. ncbi request reprint The quest for a new vaccine against tuberculosis
    Giovanni Delogu
    Institute of Microbiology, Catholic University of the Sacred Hearth, Largo A Gemelli, Rome, Italy
    J Infect Dev Ctries 3:5-15. 2009
    ..This review highlights the different strategies that are being implemented for the development of an improved vaccine against TB, the rationale behind them and the potential and feasible vaccination schedules that could be implemented...
  2. ncbi request reprint The spectrum of tuberculosis infection: new perspectives in the era of biologics
    Giovanni Delogu
    Address correspondence to Prof Delogu, Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Largo A Gemelli, 8 00168 Rome, Italy E mail
    J Rheumatol Suppl 91:11-6. 2014
    ....
  3. ncbi request reprint Impact of structural domains of the heparin binding hemagglutinin of Mycobacterium tuberculosis on function
    Giovanni Delogu
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Rome, Italy
    Protein Pept Lett 19:1035-9. 2012
    ..In this report, we will focus our attention on what is known about the structure of the HBHA protein and the protein function and role in TB pathogenesis...
  4. ncbi request reprint Microbiological and immunological diagnosis of tuberculous spondylodiscitis
    G Delogu
    Institute of Microbiology, School of Medicine, Catholic University of the Sacred Heart, Rome, Italy
    Eur Rev Med Pharmacol Sci 16:73-8. 2012
    ..Definitive diagnosis of tuberculous spondylodiscitis requires the identification of Mycobacterium tuberculosis in the biological sample following microbiological analysis...
  5. ncbi request reprint PE_PGRS proteins are differentially expressed by Mycobacterium tuberculosis in host tissues
    Giovanni Delogu
    Institute of Microbiology, Catholic University of Sacred Heart, L go A Gemelli, 8, 00168 Rome, Italy
    Microbes Infect 8:2061-7. 2006
    ..The results of this study indicate that M. tuberculosis can differentially regulate expression of PE_PGRS genes and that genes such as rv0746 and rv1651c are significantly induced while M. tuberculosis persists in host cells and tissues...
  6. ncbi request reprint Rv1818c-encoded PE_PGRS protein of Mycobacterium tuberculosis is surface exposed and influences bacterial cell structure
    Giovanni Delogu
    Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43 B, 07100 Sassari, Italy
    Mol Microbiol 52:725-33. 2004
    ..Regulated expression of PE genes could have implications for the survival and pathogenesis of mycobacteria within the human host and in other environmental niches...
  7. ncbi request reprint Expression and purification of recombinant methylated HBHA in Mycobacterium smegmatis
    Giovanni Delogu
    Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43 B, 07100 Sassari
    FEMS Microbiol Lett 239:33-9. 2004
    ..The use of fast-growing strains of M. smegmatis to obtain significant amounts of purified HBHA protein within a short timeframe, should be an effective strategy for the evaluation of a new HBHA-based vaccine candidate for tuberculosis...
  8. pmc The hbhA gene of Mycobacterium tuberculosis is specifically upregulated in the lungs but not in the spleens of aerogenically infected mice
    Giovanni Delogu
    Institute of Microbiology, Catholic University of the Sacred Hearth, Largo Gemelli 8, 00168 Rome, Italy
    Infect Immun 74:3006-11. 2006
    ....
  9. ncbi request reprint Heparin binding haemagglutin as potential diagnostic marker of Mycobacterium bovis
    Paola Molicotti
    Department of Biomedical Sciences, University of Sassari, Italy
    New Microbiol 31:423-7. 2008
    ..Our results indicated that HBHA is a potential candidate for the development of a serological assay for rapid diagnosis of cattle infected with M. bovis...
  10. ncbi request reprint Functional dissection of protein domains involved in the immunomodulatory properties of PE_PGRS33 of Mycobacterium tuberculosis
    Antonella Zumbo
    Institute of Microbiology, Universita Cattolica del Sacro Cuore, Rome, Italy
    Pathog Dis 69:232-9. 2013
    ..These results indicate that PE_PGRS33 is a protein with immunomodulatory activity and that protein stability and localization on the mycobacterial surface can affect these properties. ..
  11. doi request reprint PE_PGRS30 is required for the full virulence of Mycobacterium tuberculosis
    Raffaella Iantomasi
    Institutes of Microbiology Physics, Universita Cattolica del Sacro Cuore, Rome, Italy
    Cell Microbiol 14:356-67. 2012
    ..smegmatis, clearly demonstrating that PE_PGRS30 is an Mtb virulence factor...
  12. pmc Surface expression of MPT64 as a fusion with the PE domain of PE_PGRS33 enhances Mycobacterium bovis BCG protective activity against Mycobacterium tuberculosis in mice
    Michela Sali
    Institute of Microbiology, Catholic University, Largo A Gemelli 8, 00168 Rome, Italy
    Infect Immun 78:5202-13. 2010
    ....
  13. doi request reprint Evaluation of the anti-tuberculosis activity generated by different multigene DNA vaccine constructs
    Michela Sali
    Institute of Microbiology, Catholic University of the Sacred Heart, Largo A Gemelli 8, 00168 Rome, Italy
    Microbes Infect 10:605-12. 2008
    ..We conclude that the activity of a DNA vaccine based on tPA85B-ES6 cannot be enhanced by broadening the antigen repertoire with other highly immunogenic secreted Mtb proteins...
  14. doi request reprint PPE_MPTR genes are differentially expressed by Mycobacterium tuberculosis in vivo
    Silvia Soldini
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, L go A Gemelli, 8, 00168 Rome, Italy
    Tuberculosis (Edinb) 91:563-8. 2011
    ..tuberculosis infection, suggesting that each gene responds independently to the different and complex environmental signals encountered in host tissues...
  15. doi request reprint Lysophosphatidic acid enhances antimycobacterial response during in vivo primary Mycobacterium tuberculosis infection
    Giovanni Delogu
    Institute of Microbiology, Catholic University of the Sacred Heart, 00168 Rome, Italy
    Cell Immunol 271:1-4. 2011
    ..Altogether, these results show that LPA treatment can exert protective effect if administrated during primary infection, only...
  16. pmc Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders
    Giovanni Delogu
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Roma, Italy
    PLoS ONE 6:e18315. 2011
    ..Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT...
  17. doi request reprint Tuberculosis patients are characterized by a low-IFN-γ/high-TNF-α response to methylated HBHA produced in M. smegmatis
    Paola Molicotti
    Department of Biomedical Sciences, University of Sassari, Sassari, Italy
    Diagn Microbiol Infect Dis 71:449-52. 2011
    ..These results underscore the usefulness of HBHA in helping to distinguish LTBI subjects versus TB patients...
  18. pmc Patients with pulmonary tuberculosis develop a strong humoral response against methylated heparin-binding hemagglutinin
    Stefania Zanetti
    Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy
    Clin Diagn Lab Immunol 12:1135-8. 2005
    ..Conversely, purified methylated HBHA was strongly recognized by sera obtained from TB patients compared to controls...
  19. ncbi request reprint QuantiFERON TB Gold: a new method for latent tuberculosis infection
    Alessandra Bua
    Department of Biomedical Sciences, University of Sassari, Italy
    New Microbiol 30:477-80. 2007
    ..tuberculosis and its use alongside traditional diagnostic techniques may be an important instrument for controlling tuberculosis...
  20. doi request reprint Immune response to influenza A(H1N1)v in HIV-infected patients
    Paola Sansonetti
    Catholic University of Sacred Heart, Rome, Italy
    J Infect Dev Ctries 8:101-9. 2014
    ..HIV infection is considered a risk factor for severe outcomes of influenza A(H1N1)v infection. However, data on immune response against influenza A(H1N1)v virus in HIV-infected patients are lacking...
  21. pmc ESX-1 dependent impairment of autophagic flux by Mycobacterium tuberculosis in human dendritic cells
    Alessandra Romagnoli
    Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani, Rome, Italy
    Autophagy 8:1357-70. 2012
    ....
  22. ncbi request reprint Molecular characterization of Sardinian Mycobacterium tuberculosis isolates by IS6110 restriction fragment length polymorphism, MIRU-VNTR and rep-PCR
    Speranza Masala
    Department of Biomedical Sciences, University of Sassari
    New Microbiol 33:155-62. 2010
    ..For superior discrimination, ease of comparison of results and lower cost, MIRU-VNTR typing should be the favored PCR-based typing tool...
  23. ncbi request reprint Specific T cells restore the autophagic flux inhibited by Mycobacterium tuberculosis in human primary macrophages
    Elisa Petruccioli
    Translational Research Unit, University of Rome Tor Vergata, Rome, Italy
    J Infect Dis 205:1425-35. 2012
    ..tuberculosis-mediated autophagy. The aim of this study was to evaluate the impact of M. tuberculosis on autophagy in human primary macrophages and the role of specific T cells in this process...
  24. ncbi request reprint HIV-infected patients show impaired cellular immune response to influenza vaccination compared to healthy subjects
    Massimiliano Fabbiani
    Institute of Clinical Infectious Diseases, Catholic University of Sacred Heart, Largo Agostino Gemelli 8, 00168 Rome, Italy
    Vaccine 31:2914-8. 2013
    ..In conclusion, in HIV-infected patients influenza vaccination could have good efficacy in sustaining humoral response but cellular response appeared impaired...
  25. pmc M tuberculosis in the adjuvant modulates time of appearance of CNS-specific effector T cells in the spleen through a polymorphic site of TLR2
    Chiara Nicolò
    Institute of General Pathology, Universita Cattolica del S Cuore, Rome, Italy
    PLoS ONE 8:e55819. 2013
    ..Thus, pathogens engaging TLR2 on T cells regulate directly T-cell trafficking, and polymorphisms of TLR2 condition T-cell trafficking upon a limiting concentration of ligand...
  26. ncbi request reprint Homologous prime boosting based on intranasal delivery of non-pathogenic invasive Escherichia coli expressing MPT64, decreases Mycobacterium tuberculosis dissemination
    Michela Sali
    Institute of Microbiology, Catholic University of the Sacred Hearth, Largo Gemelli 8, 00168 Rome, Italy
    Vaccine 32:4051-8. 2014
    ..Our data suggest that replication-deficient invasive E. coli may represent a suitable platform for BCG/rBCG priming followed by homologous-boosting immunization strategies. ..
  27. doi request reprint Exploiting the mycobacterial cell wall to design improved vaccines against tuberculosis
    Matteo Morandi
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Rome, Italy
    J Infect Dev Ctries 7:169-81. 2013
    ....
  28. pmc Role of AFR1, an ABC transporter-encoding gene, in the in vivo response to fluconazole and virulence of Cryptococcus neoformans
    Maurizio Sanguinetti
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Largo F Vito 1, Rome 00168, Italy
    Infect Immun 74:1352-9. 2006
    ..These findings indicate that the upregulation of the AFR1 gene is an important factor in either determining the in vivo resistance to fluconazole or influencing the virulence of C. neoformans...
  29. pmc Interferon regulatory factor 8-deficiency determines massive neutrophil recruitment but T cell defect in fast growing granulomas during tuberculosis
    Stefano Rocca
    Department of Veterinary Medicine, University of Sassari, Sassari, Italy
    PLoS ONE 8:e62751. 2013
    ....
  30. doi request reprint Mycobacterium smegmatis expressing a chimeric protein MPT64-proteolipid protein (PLP) 139-151 reorganizes the PLP-specific T cell repertoire favoring a CD8-mediated response and induces a relapsing experimental autoimmune encephalomyelitis
    Chiara Nicolò
    Institute of General Pathology, Catholic University, Rome, Italy
    J Immunol 184:222-35. 2010
    ..The composition of the self-reactive repertoire activated determines severity and histology of the resulting disease...
  31. ncbi request reprint [An integrated model for the evaluation and risk management of tuberculosis infection and active tuberculosis in homeless people in the city of Rome (Italy)]
    Antonio Gerardo Cairo
    Istituto di Igiene, Universita Cattolica del Sacro Cuore, Facoltà di Medicina e Chirurgia Agostino Gemelli, Largo Francesco Vito, Roma
    Ig Sanita Pubbl 64:431-45. 2008
    ....
  32. pmc Natural lysophospholipids reduce Mycobacterium tuberculosis-induced cytotoxicity and induce anti-mycobacterial activity by a phagolysosome maturation-dependent mechanism in A549 type II alveolar epithelial cells
    Emanuela Greco
    Department of Biology, University of Rome Tor Vergata, Rome, Italy
    Immunology 129:125-32. 2010
    ....
  33. doi request reprint Prospective evaluation of epidemiological, clinical, and microbiological features of pandemic influenza A (H1N1) virus infection in Italy
    Massimiliano Fabbiani
    Institute of Clinical Infectious Diseases, Catholic University of Sacred Heart, Rome, Italy
    J Med Virol 83:2057-65. 2011
    ..Such findings might contribute to improve clinical and epidemiological management of the disease...
  34. ncbi request reprint Identification of mycobacterial infections in wild boars in Northern Sardinia, Italy
    Stefania Zanetti
    Department of Biomedical Sciences, University of Sassari, Sassari, Italy
    Acta Vet Hung 56:145-52. 2008
    ....
  35. ncbi request reprint Neonatal tuberculosis: an experience that teaches
    Costantino Romagnoli
    Department of Paediatrics, Catholic University, A Gemelli University Hospital, Rome, Italy
    J Matern Fetal Neonatal Med 25:38-41. 2012
    ..This study reports on an experience following contact by a large number of newborn babies with a nurse infected by active tuberculosis (TB)...
  36. doi request reprint A twenty-year retrospective study of pediatric tuberculosis in two tertiary hospitals in Rome
    Danilo Buonsenso
    Catholic University of Sacred Heart A Gemelli Hospital, Department of Pediatrics, Pediatric Infectious Disease Unit, Largo A Gemelli 8, 00168 Rome, Italy
    Pediatr Infect Dis J 31:1022-6. 2012
    ..Tuberculosis (TB) is among the top 10 causes of child death worldwide. Nevertheless, childhood disease has been neglected by tuberculosis control programs...
  37. pmc Tuberculosis in sheltered homeless population of Rome: an integrated model of recruitment for risk management
    Patrizia Laurenti
    Department of Hygiene, Universita Cattolica del Sacro Cuore, Largo Francesco Vito, 00168 Rome, Italy
    ScientificWorldJournal 2012:396302. 2012
    ..19). This approach, based on an integrated social network, guarantees high adherence to screening (89.93%), allowing patients testing positive for latent tuberculosis infection to be diagnosed and rapidly referred to a specialized center...
  38. doi request reprint In vitro effect of unacylated ghrelin and obestatin on human luteal cell function
    Federica Romani
    Cattedra di Fisiopatologia della Riproduzione Umana, Universita Cattolica del Sacro Cuore, Rome, Italy
    Fertil Steril 97:991-6. 2012
    ....
  39. doi request reprint Immune response to influenza A (H1N1)v monovalent MF59-adjuvanted vaccine in HIV-infected patients
    Massimiliano Fabbiani
    Institute of Clinical Infectious Diseases, Catholic University of Sacred Heart, Largo F Vito 1, 00168 Rome, Italy
    Vaccine 29:2836-9. 2011
    ..These results underscore the usefulness of immunization against influenza in HIV-infected patients, though a boosting dose of vaccine may be required in seriously immunocompromised patients...
  40. pmc The mycobacterial heparin-binding hemagglutinin is a protective antigen in the mouse aerosol challenge model of tuberculosis
    Marcela Parra
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bldg 29, Rm 503, HFM 431, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Infect Immun 72:6799-805. 2004
    ..tuberculosis are critical for generating effective host immune responses. The vaccine studies described here demonstrate that HBHA is a promising new vaccine candidate for tuberculosis...
  41. ncbi request reprint Methylation-dependent T cell immunity to Mycobacterium tuberculosis heparin-binding hemagglutinin
    Stephane Temmerman
    Laboratory of Immunology, Erasme Hospital, Universite Libre de Bruxelles, Route de Lennik, 808, B 1070 Brussels, Belgium
    Nat Med 10:935-41. 2004
    ..Thus, post-translational modifications of proteins may be crucial for their ability to induce protective T cell-mediated immunity against infectious diseases such as tuberculosis...
  42. ncbi request reprint The PE multigene family: a 'molecular mantra' for mycobacteria
    Michael J Brennan
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Building 29 Room 502, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Trends Microbiol 10:246-9. 2002
    ....
  43. doi request reprint Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli
    Paola Brun
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, 35121 Padua, Italy
    Vaccine 26:1934-41. 2008
    ..Both DNA vaccines were able to induce an antigen-specific T-cell response. Moreover, mice immunized with the recombinant bacteria carrying the DNA vaccine encoding HtpX, were significatively protected from challenge with M. tuberculosis...
  44. ncbi request reprint Expression of the PE_PGRS 33 protein in Mycobacterium smegmatis triggers necrosis in macrophages and enhanced mycobacterial survival
    Veerabadran Dheenadhayalan
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Building 29, Room 503, HFM 431, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Microbes Infect 8:262-72. 2006
    ....
  45. pmc Posttranslational regulation of Mycobacterium tuberculosis extracytoplasmic-function sigma factor sigma L and roles in virulence and in global regulation of gene expression
    Elisa Dainese
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35100 Padova, Italy
    Infect Immun 74:2457-61. 2006
    ..Several of these genes could mediate important cell envelope-related processes. Importantly, a sigL-rslA mutant strain was significantly attenuated in a mouse model of infection...
  46. pmc Variable expression patterns of Mycobacterium tuberculosis PE_PGRS genes: evidence that PE_PGRS16 and PE_PGRS26 are inversely regulated in vivo
    Veerabadran Dheenadhayalan
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    J Bacteriol 188:3721-5. 2006
    ..tuberculosis. Variable expression of PE_PGRS proteins could have implications for their role in the immunopathogenesis of tuberculosis...
  47. pmc DNA vaccine combinations expressing either tissue plasminogen activator signal sequence fusion proteins or ubiquitin-conjugated antigens induce sustained protective immunity in a mouse model of pulmonary tuberculosis
    Giovanni Delogu
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Infect Immun 70:292-302. 2002
    ..In conclusion, these studies demonstrate the effectiveness of immunization with DNA vaccine combinations against tuberculosis and suggest that further testing of these plasmid cocktails is warranted...
  48. ncbi request reprint PE is a functional domain responsible for protein translocation and localization on mycobacterial cell wall
    Alessandro Cascioferro
    Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy
    Mol Microbiol 66:1536-47. 2007
    ....