Jeffrey E Gerst

Summary

Affiliation: Weizmann Institute of Science
Country: Israel

Publications

  1. pmc t-SNARE phosphorylation regulates endocytosis in yeast
    Sangiliyandi Gurunathan
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 13:1594-607. 2002
  2. pmc The yeast Batten disease orthologue Btn1 controls endosome-Golgi retrograde transport via SNARE assembly
    Rachel Kama
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Cell Biol 195:203-15. 2011
  3. ncbi request reprint SNARE regulators: matchmakers and matchbreakers
    Jeffrey E Gerst
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
    Biochim Biophys Acta 1641:99-110. 2003
  4. doi request reprint Message on the web: mRNA and ER co-trafficking
    Jeffrey E Gerst
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Trends Cell Biol 18:68-76. 2008
  5. pmc Btn2, a Hook1 ortholog and potential Batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast
    Rachel Kama
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:605-21. 2007
  6. pmc mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae
    Stella Aronov
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:3441-55. 2007
  7. pmc Different domains of the UBL-UBA ubiquitin receptor, Ddi1/Vsm1, are involved in its multiple cellular roles
    Galina Gabriely
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 19:3625-37. 2008
  8. pmc A phosphatidylinositol-transfer protein and phosphatidylinositol-4-phosphate 5-kinase control Cdc42 to regulate the actin cytoskeleton and secretory pathway in yeast
    Liat Yakir-Tamang
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 20:3583-97. 2009
  9. ncbi request reprint A genomic integration method to visualize localization of endogenous mRNAs in living yeast
    Liora Haim
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Methods 4:409-12. 2007
  10. pmc Translation- and SRP-independent mRNA targeting to the endoplasmic reticulum in the yeast Saccharomyces cerevisiae
    Judith Kraut-Cohen
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 24:3069-84. 2013

Collaborators

  • Sangiliyandi Gurunathan
  • Anne Spang
  • Cecile Brocard
  • Eitan Bibi
  • Andre Levchenko
  • Deborah Fass
  • Richard A Singer
  • Liora Haim-Vilmovsky
  • Rachel Kama
  • Gadi Zipor
  • Judith Kraut-Cohen
  • Boris Slobodin
  • Rita Gelin-Licht
  • Galina Gabriely
  • Noga Gadir
  • Stella Aronov
  • Liat Yakir-Tamang
  • Micah Robinson
  • Vydehi Kanneganti
  • Adina Weinberger
  • Liora Haim
  • Roy Sirkis
  • Antonio Castillo-Flores
  • Michael Marash
  • Elena Friedmann
  • Evgenia Afanasieva
  • Ido Yosef
  • Saurabh Paliwal
  • Patrick Conlon
  • Christian Ungermann
  • Rebecca H Herbst
  • Einat Safran
  • Lois E Murray
  • Christina Schindler
  • Gerald C Johnston
  • Pak Phi Poon
  • Faustin Kamena
  • Yehuda Salzberg
  • Shmuel Shaltiel

Detail Information

Publications31

  1. pmc t-SNARE phosphorylation regulates endocytosis in yeast
    Sangiliyandi Gurunathan
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 13:1594-607. 2002
    ..These results suggest that endocytosis is also modulated by t-SNARE phosphorylation in vivo...
  2. pmc The yeast Batten disease orthologue Btn1 controls endosome-Golgi retrograde transport via SNARE assembly
    Rachel Kama
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Cell Biol 195:203-15. 2011
    ..Thus, Btn1 controls retrograde sorting by regulating SNARE phosphorylation and assembly, a process that may be adversely affected in Batten Disease patients...
  3. ncbi request reprint SNARE regulators: matchmakers and matchbreakers
    Jeffrey E Gerst
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
    Biochim Biophys Acta 1641:99-110. 2003
    ..In this review the possible role of SNARE regulators in SNARE assembly and the involvement of SNARE phosphorylation in the regulation of intracellular membrane trafficking will be discussed...
  4. doi request reprint Message on the web: mRNA and ER co-trafficking
    Jeffrey E Gerst
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Trends Cell Biol 18:68-76. 2008
    ..e. during cell division in yeast), or enable confined spatial responses to environmental stimuli (i.e. during synaptic remodeling or in cases of neuronal injury)...
  5. pmc Btn2, a Hook1 ortholog and potential Batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast
    Rachel Kama
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:605-21. 2007
    ..Thus, Btn2 facilitates specific protein retrieval from a late endosome to the Golgi apparatus, a process which may be adversely affected in patients with Batten disease...
  6. pmc mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae
    Stella Aronov
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:3441-55. 2007
    ..Bud-localized mRNAs, including ASH1, were found to cofractionate with ER microsomes in a She2- and Sec3-dependent manner; thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast...
  7. pmc Different domains of the UBL-UBA ubiquitin receptor, Ddi1/Vsm1, are involved in its multiple cellular roles
    Galina Gabriely
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 19:3625-37. 2008
    ..g., Ddi1, Ddi1(S341A)) rescue the growth of sec9-4 cells similar to Sso1 overproduction. Thus, Ddi1 participates in multiple cellular processes via its different domains and phosphorylation may regulate exocytic functions...
  8. pmc A phosphatidylinositol-transfer protein and phosphatidylinositol-4-phosphate 5-kinase control Cdc42 to regulate the actin cytoskeleton and secretory pathway in yeast
    Liat Yakir-Tamang
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 20:3583-97. 2009
    ..Based upon these findings, we propose that the exocytic signal involves PI delivery to the PI kinases (i.e., Mss4) by Sfh5, generation of PI(4,5)P(2), and PI(4,5)P(2)-dependent regulation of Cdc42 and the actin cytoskeleton...
  9. ncbi request reprint A genomic integration method to visualize localization of endogenous mRNAs in living yeast
    Liora Haim
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Methods 4:409-12. 2007
    ..Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae)...
  10. pmc Translation- and SRP-independent mRNA targeting to the endoplasmic reticulum in the yeast Saccharomyces cerevisiae
    Judith Kraut-Cohen
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 24:3069-84. 2013
    ..Overall we show that mSMP targeting to the ER is both translation- and SRP-independent, and regulated by cis elements contained within the message and trans-acting RNA-binding proteins (e.g., She2, Puf2). ..
  11. pmc Btn3 is a negative regulator of Btn2-mediated endosomal protein trafficking and prion curing in yeast
    Vydehi Kanneganti
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 22:1648-63. 2011
    ..Therefore Btn3 is a novel negative regulator of intracellular protein sorting, which may be of importance in the onset of complex I deficiency and Batten disease in humans...
  12. pmc Scp160-dependent mRNA trafficking mediates pheromone gradient sensing and chemotropism in yeast
    Rita Gelin-Licht
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Cell Rep 1:483-94. 2012
    ..This is, to our knowledge, the first demonstration of ligand-activated RNA targeting in the development of a simple eukaryote...
  13. pmc Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast
    Michael Marash
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 14:3114-25. 2003
    ..Thus, one way by which phosphorylation inhibits SNARE assembly could be by regulating the association of inhibitory factors that control the ability of t-SNAREs to form complexes in vivo...
  14. pmc The Gcs1 Arf-GAP mediates Snc1,2 v-SNARE retrieval to the Golgi in yeast
    Micah Robinson
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 17:1845-58. 2006
    ..Together, these results suggest that Gcs1 facilitates the incorporation of the Snc v-SNAREs into COPI recycling vesicles and subsequent endosome-Golgi sorting in yeast...
  15. pmc Control of Golgi morphology and function by Sed5 t-SNARE phosphorylation
    Adina Weinberger
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Biol Cell 16:4918-30. 2005
    ..Thus, a cycle of Sed5 phosphorylation and dephosphorylation is required for normal t-SNARE function and may choreograph Golgi ordering and dispersal...
  16. pmc Localization of mRNAs coding for mitochondrial proteins in the yeast Saccharomyces cerevisiae
    Noga Gadir
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    RNA 17:1551-65. 2011
    ..Thus, endogenously expressed mRNAs are targeted to the mitochondria in vivo, and multiple factors contribute to mMP localization...
  17. pmc A genomic integration method for the simultaneous visualization of endogenous mRNAs and their translation products in living yeast
    Liora Haim-Vilmovsky
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    RNA 17:2249-55. 2011
    ..In addition, we demonstrate that tagged proteins like Hhf2, Vph1, and Yef3 localize to their expected subcellular location, while the localization of their mRNAs is revealed for the first time...
  18. pmc Localization of mRNAs coding for peroxisomal proteins in the yeast, Saccharomyces cerevisiae
    Gadi Zipor
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 106:19848-53. 2009
    ..The different patterns of mPP localization observed suggest that multiple mechanisms involved in mRNA localization and translation may play roles in the importation of protein into peroxisomes...
  19. pmc Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast
    Galina Gabriely
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:526-40. 2007
    ..Together, our findings suggest that certain COPI subunits could have a direct role in vacuolar protein sorting to the MVB compartment...
  20. pmc A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes
    Boris Slobodin
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    RNA 16:2277-90. 2010
    ..Finally, RaPID could also be used to show biochemically the coexistence of different RNA species within the same RNP complex (e.g., coprecipitation of the yeast SRO7, WSC2, SEC3, and IST2 mRNAs with ASH1 mRNA) for the first time...
  21. ncbi request reprint YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins
    Elena Friedmann
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 277:35274-81. 2002
    ..As Yos9 is not a component of the Emp24 complex, it may act as a novel escort factor for GPI-anchored proteins in ER-Golgi transport in yeast and possibly in mammals...
  22. ncbi request reprint Involvement of the late secretory pathway in actin regulation and mRNA transport in yeast
    Stella Aronov
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 279:36962-71. 2004
    ..Our novel observations suggest that vesicular transport regulates the actin cytoskeleton in yeast (and not just vice versa) leading to subsequent defects in mRNA transport and localization...
  23. doi request reprint Isolation of mRNAs encoding peroxisomal proteins from yeast using a combined cell fractionation and affinity purification procedure
    Gadi Zipor
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Methods Mol Biol 714:323-33. 2011
    ..The method is presented for the analysis of peroxisome-associated mRNAs; however it is applicable to studies on other subcellular compartments...
  24. ncbi request reprint Mso1 is a novel component of the yeast exocytic SNARE complex
    Antonio Castillo-Flores
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 280:34033-41. 2005
    ..These results suggest that Mso1 is a component of the exocytic SNARE complex and a possible ortholog of the Munc13/Mint proteins...
  25. doi request reprint RaPID: an aptamer-based mRNA affinity purification technique for the identification of RNA and protein factors present in ribonucleoprotein complexes
    Boris Slobodin
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Methods Mol Biol 714:387-406. 2011
    ..This method employs the MS2 coat RBP fused to both GFP and streptavidin-binding protein to precipitate MS2 aptamer-tagged RNAs using immobilized streptavidin...
  26. doi request reprint Phosphoinositides, exocytosis and polarity in yeast: all about actin?
    Liat Yakir-Tamang
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Trends Cell Biol 19:677-84. 2009
    ....
  27. doi request reprint Visualizing endogenous mRNAs in living yeast using m-TAG, a PCR-based RNA aptamer integration method, and fluorescence microscopy
    Liora Haim-Vilmovsky
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Methods Mol Biol 714:237-47. 2011
    ..This method allows for the easy examination of mRNA localization using fluorescence microscopy and leaves the yeast cells amenable for further genetic analysis...
  28. doi request reprint m-TAG: a PCR-based genomic integration method to visualize the localization of specific endogenous mRNAs in vivo in yeast
    Liora Haim-Vilmovsky
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Nat Protoc 4:1274-84. 2009
    ..The m-TAG procedure is simple, easy to perform and takes <3 weeks to yield cultured yeast strains for mRNA visualization...
  29. ncbi request reprint Ddi1, a eukaryotic protein with the retroviral protease fold
    Roy Sirkis
    Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    J Mol Biol 364:376-87. 2006
    ....
  30. pmc Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast
    Sangiliyandi Gurunathan
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    EMBO J 21:602-14. 2002
    ..e. VPS34 and VAM3, respectively) have no effect. Thus, one branch of the secretory pathway in yeast involves an intermediate sorting compartment and has a specific requirement for clathrin and a dynamin-related protein in SV biogenesis...
  31. doi request reprint Addressing mRNAs to the ER: cis sequences act up!
    Judith Kraut-Cohen
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Trends Biochem Sci 35:459-69. 2010
    ..Furthermore, translation-independent cis-acting sequence elements present in both mCPs and mSMPs appear to govern the ability of mRNAs to associate with ER. Thus, a more complex picture of how and why mRNAs target the ER is emerging...