Yoram Gerchman

Summary

Affiliation: University of Haifa
Location: Tivon, Israel

Publications

  1. ncbi request reprint Replacements of histidine 226 of NhaA-Na+/H+ antiporter of Escherichia coli. Cysteine (H226C) or serine (H226S) retain both normal activity and pH sensitivity, aspartate (H226D) shifts the pH profile toward basic pH, and alanine (H226A) inactivates the ca
    A Rimon
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    J Biol Chem 270:26813-7. 1995
  2. pmc Distinct antimicrobial activities in aphid galls on Pistacia atlantica
    Yoram Gerchman
    Department of Biology and Environment, University of Haifa, Oranim, Israel
    Plant Signal Behav 6:2008-12. 2011
  3. pmc Melatonin as an antioxidant and its semi-lunar rhythm in green macroalga Ulva sp
    Ofir Tal
    The Israeli Center for Interdisciplinary Research in Chronobiology, Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, 31905 Haifa, Israel
    J Exp Bot 62:1903-10. 2011
  4. ncbi request reprint A synthetic multicellular system for programmed pattern formation
    Subhayu Basu
    Department of Electrical Engineering, Princeton University, Princeton, New Jersey 08544, USA
    Nature 434:1130-4. 2005
  5. pmc Teaching bacteria a new language
    Yoram Gerchman
    Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 101:2221-2. 2004
  6. ncbi request reprint Oligomerization of NhaA, the Na+/H+ antiporter of Escherichia coli in the membrane and its functional and structural consequences
    Y Gerchman
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochemistry 40:3403-12. 2001
  7. ncbi request reprint Na(+)/H(+) antiporters
    E Padan
    Department of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochim Biophys Acta 1505:144-57. 2001
  8. ncbi request reprint The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli
    M Venturi
    Max Planck Institut fur Biophysik, Abteilung Molekulare Membranbiologie, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt Main, Germany
    J Biol Chem 275:4734-42. 2000
  9. ncbi request reprint A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside
    Y Gerchman
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 274:24617-24. 1999
  10. ncbi request reprint The molecular mechanism of regulation of the NhaA Na+/H+ antiporter of Escherichia coli, a key transporter in the adaptation to Na+ and H+
    E Padan
    Hebrew University of Jerusalem, Department of Microbial and Molecular Ecology, Jerusalem, Israel
    Novartis Found Symp 221:183-96; discussion 196-9. 1999

Collaborators

Detail Information

Publications15

  1. ncbi request reprint Replacements of histidine 226 of NhaA-Na+/H+ antiporter of Escherichia coli. Cysteine (H226C) or serine (H226S) retain both normal activity and pH sensitivity, aspartate (H226D) shifts the pH profile toward basic pH, and alanine (H226A) inactivates the ca
    A Rimon
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    J Biol Chem 270:26813-7. 1995
    ..It is suggested that charge modifies the pH profile but is not essential for the pH regulation of NhaA...
  2. pmc Distinct antimicrobial activities in aphid galls on Pistacia atlantica
    Yoram Gerchman
    Department of Biology and Environment, University of Haifa, Oranim, Israel
    Plant Signal Behav 6:2008-12. 2011
    ..Our results provide evidence for the protective antimicrobial role of galls. This remarkable antibacterial and antifungal activity in the galls of S. wertheimae may be of agricultural and pharmaceutical value...
  3. pmc Melatonin as an antioxidant and its semi-lunar rhythm in green macroalga Ulva sp
    Ofir Tal
    The Israeli Center for Interdisciplinary Research in Chronobiology, Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, 31905 Haifa, Israel
    J Exp Bot 62:1903-10. 2011
    ..These results provide, for the first time, experimental data that support both an antioxidant role for melatonin and its semi-lunar rhythm in macroalgae...
  4. ncbi request reprint A synthetic multicellular system for programmed pattern formation
    Subhayu Basu
    Department of Electrical Engineering, Princeton University, Princeton, New Jersey 08544, USA
    Nature 434:1130-4. 2005
    ....
  5. pmc Teaching bacteria a new language
    Yoram Gerchman
    Department of Electrical Engineering, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 101:2221-2. 2004
  6. ncbi request reprint Oligomerization of NhaA, the Na+/H+ antiporter of Escherichia coli in the membrane and its functional and structural consequences
    Y Gerchman
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochemistry 40:3403-12. 2001
    ..The results are consistent with homooligomeric NhaA and the location of residue 254 in the interface between monomers. Intermolecular cross-linking of V254C caused an acidic shift in the pH profile of NhaA...
  7. ncbi request reprint Na(+)/H(+) antiporters
    E Padan
    Department of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochim Biophys Acta 1505:144-57. 2001
    ..It is envisaged that interdisciplinary approaches combining structure, molecular and cell biology as well as genomics should be applied in the future to the study of this important group of transporters...
  8. ncbi request reprint The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli
    M Venturi
    Max Planck Institut fur Biophysik, Abteilung Molekulare Membranbiologie, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt Main, Germany
    J Biol Chem 275:4734-42. 2000
    ....
  9. ncbi request reprint A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside
    Y Gerchman
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 274:24617-24. 1999
    ..Taken together these results imply the involvement of loop VIII-IX in the pH-induced conformational change, which leads to activation of NhaA at alkaline pH...
  10. ncbi request reprint The molecular mechanism of regulation of the NhaA Na+/H+ antiporter of Escherichia coli, a key transporter in the adaptation to Na+ and H+
    E Padan
    Hebrew University of Jerusalem, Department of Microbial and Molecular Ecology, Jerusalem, Israel
    Novartis Found Symp 221:183-96; discussion 196-9. 1999
    ..We suggest that the pattern of regulation of nhaA found in E. coli is a paradigm for the response of proteins and genes to H+ and Na+, the most common ions that challenge every cell...
  11. ncbi request reprint A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli
    A Rimon
    Division of Microbial and Molecular Ecology, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 273:26470-6. 1998
    ..Three of the four suppressor mutations cluster in helix IV, whereas the original mutation is in helix XI, suggesting that the two helixes interact...
  12. ncbi request reprint Probing the conformation of NhaA, a Na+/H+ antiporter from Escherichia coli, with trypsin
    A Rothman
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochemistry 36:14572-6. 1997
    ..Thus, upon activation, NhaA appears to undergo a change in conformation that is reflected in specific regions of the protein...
  13. ncbi request reprint Histidine 225, a residue of the NhaA-Na+/H+ antiporter of Escherichia coli is exposed and faces the cell exterior
    Y Olami
    Division of Microbial and Molecular Ecology, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 272:1761-8. 1997
    ..These results strongly suggest that H225C is exposed at the periplasmic face of the membrane...
  14. pmc Histidine-226 is part of the pH sensor of NhaA, a Na+/H+ antiporter in Escherichia coli
    Y Gerchman
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    Proc Natl Acad Sci U S A 90:1212-6. 1993
    ..5, H226R is reversibly inactivated above pH 7.5, reaching 10-20% of the maximal activity at pH 8.5. We suggest that His-226 is part of a pH-sensitive site that regulates the activity of NhaA...
  15. doi request reprint Lipolytic, proteolytic, and cholesterol-degrading bacteria from the human cerumen
    Yoram Gerchman
    Department of Biology and Environment, University of Haifa in Oranim, Haifa, Israel
    Curr Microbiol 64:588-91. 2012
    ..and, interestingly, multiple Bacillus spp.) showing that many of these bacteria harbor biochemical traits enabling them to utilize different cerumen components for their growth. We also suggest the existence of microbial consortia...