Research Topics
Genomes and Genes | Abraham LoyterSummaryAffiliation: The Hebrew University Country: Israel Publications
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Publications
Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Virol J 7:177. 2010..In the absence of LEDGF/p75 very little integration and virus production can be detected, as was demonstrated using LEDGF/p75-knockdown cells...- Specific eradication of HIV-1 from infected cultured cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
AIDS Res Ther 7:31. 2010..It is our view that the results described in this work suggest a novel approach to specifically promote death of HIV-1 infected cells and thus may eventually be developed into a new and general anti-viral therapy... 
Mechanism of action of the HIV-1 integrase inhibitory peptide LEDGF 361-370Zvi Hayouka
Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Biochem Biophys Res Commun 394:260-5. 2010..We conclude that the full LEDGF 361-370 peptide is a potent HIV-1 inhibitor and may be used for further development as an anti-HIV lead compound...- Stimulation of the HIV-1 integrase enzymatic activity and cDNA integration by a peptide derived from the integrase proteinAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Biopolymers 93:740-51. 2010..These results suggest that the stimulatory effect of INS in wild-type virus-infected cells is due to a dual effect: it dissociates the inactive Rev-IN complex and directly activates the free IN... - Inhibition of HIV-1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signalAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Retrovirology 6:112. 2009..The exact mechanisms underlying the nuclear import of IN and especially the question of whether it bears a functional nuclear localization signal (NLS) remain controversial... - Correlation between shiftide activity and HIV-1 integrase inhibition by a peptide selected from a combinatorial libraryAyelet Armon-Omer
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
J Mol Biol 376:971-82. 2008..Thus, the results show a distinct correlation between the ability of the selected peptides to inhibit IN activity and that to shift its oligomerization equilibrium... - Inhibiting HIV-1 integrase by shifting its oligomerization equilibriumZvi Hayouka
Department of Organic Chemistry, Department of Biological Chemistry, Protein Purification Unit, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
Proc Natl Acad Sci U S A 104:8316-21. 2007..These peptides are promising anti-HIV lead compounds that modulate oligomerization of IN via a previously uncharacterized mechanism, which bears advantages over the conventional interface dimerization inhibitors... - Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteinsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Mol Med 16:34-44. 2010..Our findings demonstrate that integration of HIV-1 cDNA is regulated by an interplay between viral Rev and the host-cell LEDGF/p75 proteins... - Peptides that bind the HIV-1 integrase and modulate its enzymatic activity--kinetic studies and mode of actionAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
FEBS J 278:316-30. 2011..The IN-stimulating peptide bound IN in several specific sites that did not bind any of the inhibitory peptides. This may account for its unique activity... 
Interaction between HIV-1 Rev and integrase proteins: a basis for the development of anti-HIV peptidesJoseph Rosenbluh
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, and Department of Organic Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
J Biol Chem 282:15743-53. 2007..The present work describes novel anti-HIV-1 lead peptides that inhibit viral replication in cultured cells by blocking DNA integration in vivo...- Cyclic peptide inhibitors of HIV-1 integrase derived from the LEDGF/p75 proteinZvi Hayouka
Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Israel
Bioorg Med Chem 18:8388-95. 2010..The NMR structure of c(MZ 4-1) showed that it obtains a bioactive conformation that is similar to the parent site in LEDGF/p75... - Peptide inhibitors of HIV-1 integrase: from mechanistic studies to improved lead compoundsMichal Maes
Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
Bioorg Med Chem 17:7635-42. 2009..Mapping the peptide binding sites in IN provided a model that explains this difference. We conclude that IN1 1-10 is an improved lead compound for further development of IN inhibitors... - Peptides derived from HIV-1 integrase that bind Rev stimulate viral genome integrationAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
PLoS ONE 4:e4155. 2009..This ordered sequence of events, centered on integration, is mandatory for HIV replication... - Peptides derived from HIV-1 Rev inhibit HIV-1 integrase in a shiftide mechanismZvi Hayouka
Institute of Chemistry, Department of Biological Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
Biopolymers 90:481-7. 2008..We conclude that protein-protein interactions of IN may serve as a general valuable source for shiftide design... - A novel role for the viral Rev protein in promoting resistance to superinfection by human immunodeficiency virus type 1Aviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
J Gen Virol 91:1503-13. 2010..The involvement of Rev, expressed from either unintegrated or integrated viral cDNA, in promoting SIR was clearly demonstrated. We conclude that SIR involves Rev-dependent control of HIV cDNA integration... - Permeabilized mammalian cells as an experimental system for nuclear import of geminiviral karyophilic proteins and of synthetic peptides derived from their nuclear localization signal regionsGideon Kass
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
J Gen Virol 87:2709-20. 2006..BV1-NLS and CP-NLS were shown to mediate specific binding to importin alpha, both in vitro and in vivo. These results are consistent with a common nuclear-import pathway for CP and BV1, probably via importin alpha... - Novel regulation of HIV-1 replication and pathogenicity: Rev inhibition of integrationAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Protein Eng Des Sel 22:753-63. 2009..Our results thus demonstrate a new regulatory function of Rev and probably establish a link between Rev restriction of HIV-1 integration and protection of HIV-1-infected cells from premature cell death... - Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
Virus Genes 40:341-6. 2010..Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells' death... - Functional analysis of backbone cyclic peptides bearing the arm domain of the HIV-1 Rev protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expressionElana Hariton-Gazal
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9104, Israel
Biochemistry 44:11555-66. 2005..The potential of BC peptides bearing ARM domains as lead compounds for the production of anti-HIV drugs is discussed... - A synthetic peptide bearing the HIV-1 integrase 161-173 amino acid residues mediates active nuclear import and binding to importin alpha: characterization of a functional nuclear localization signalAyelet Armon-Omer
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
J Mol Biol 336:1117-28. 2004..The NLS(IN) as well as the full-length IN protein interacted specifically with importin alpha, binding of which was blocked by the NLS(IN) peptide itself as well as by the NLS-SV40... - Translocation of histone proteins across lipid bilayers and Mycoplasma membranesJoseph Rosenbluh
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem 91904, Jerusalem, Israel
J Mol Biol 345:387-400. 2005..Based on the present and previous data histones appear to be characterized by the same features that characterize cell penetrating peptides and proteins (CPPs)... - Positively charged peptides can interact with each other, as revealed by solid phase binding assaysJoseph Rosenbluh
Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Anal Biochem 352:157-68. 2006..We discuss the relevance of our findings for the use of solid phase assay systems to study interactions between biomolecules... - Non-endocytic penetration of core histones into petunia protoplasts and cultured cells: a novel mechanism for the introduction of macromolecules into plant cellsJoseph Rosenbluh
Department of Biological Chemistry, The Alexander Silberman Institute of Life, Sciences, The Hebrew University of Jerusalem, 91904, Israel
Biochim Biophys Acta 1664:230-40. 2004..To the best of our knowledge, the findings of the present paper demonstrate, for the first time, the activity of cell penetrating proteins (CPPs) in plant cells... - Strategies to inhibit viral protein nuclear import: HIV-1 as a targetAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Biochim Biophys Acta 1813:1646-53. 2011..This article is part of a Special Issue entitled: Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import... - Transportin 3 and importin α are required for effective nuclear import of HIV-1 integrase in virus-infected cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
Nucleus 1:422-31. 2010.... - The plant VirE2 interacting protein 1. a molecular link between the Agrobacterium T-complex and the host cell chromatin?Abraham Loyter
Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Plant Physiol 138:1318-21. 2005 - A structural model of the HIV-1 Rev-integrase complex: the molecular basis of integrase regulation by RevHadar Benyamini
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Biochem Biophys Res Commun 416:252-7. 2011..Our model is expected to contribute to the development of lead compounds that inhibit the Rev-IN interaction and thus lead to multi-integration of viral cDNA and consequently to apoptosis of HIV-1 infected cells... - Inhibition of nuclear import mediated by the Rev-arginine rich motif by RNA moleculesKonstantin Fineberg
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Biochemistry 42:2625-33. 2003..The implication of our results for the regulation of the nuclear import of Rev as well as for the use of RNA molecules as antiviral drugs is discussed...