Abraham Loyter

Summary

Affiliation: The Hebrew University
Country: Israel

Publications

  1. pmc Specific eradication of HIV-1 from infected cultured cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    AIDS Res Ther 7:31. 2010
  2. pmc Transportin 3 and importin α are required for effective nuclear import of HIV-1 integrase in virus-infected cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Nucleus 1:422-31. 2010
  3. doi request reprint Mechanism of action of the HIV-1 integrase inhibitory peptide LEDGF 361-370
    Zvi Hayouka
    Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Biochem Biophys Res Commun 394:260-5. 2010
  4. pmc Inhibiting HIV-1 integrase by shifting its oligomerization equilibrium
    Zvi Hayouka
    Department of Organic Chemistry, Department of Biological Chemistry, Protein Purification Unit, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 104:8316-21. 2007
  5. ncbi request reprint Stimulation of the HIV-1 integrase enzymatic activity and cDNA integration by a peptide derived from the integrase protein
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biopolymers 93:740-51. 2010
  6. doi request reprint Correlation between shiftide activity and HIV-1 integrase inhibition by a peptide selected from a combinatorial library
    Ayelet Armon-Omer
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Mol Biol 376:971-82. 2008
  7. doi request reprint Peptides that bind the HIV-1 integrase and modulate its enzymatic activity--kinetic studies and mode of action
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    FEBS J 278:316-30. 2011
  8. pmc Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Virol J 7:177. 2010
  9. pmc Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteins
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Mol Med 16:34-44. 2010
  10. ncbi request reprint Peptides derived from HIV-1 Rev inhibit HIV-1 integrase in a shiftide mechanism
    Zvi Hayouka
    Institute of Chemistry, Department of Biological Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Biopolymers 90:481-7. 2008

Collaborators

Detail Information

Publications30

  1. pmc Specific eradication of HIV-1 from infected cultured cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    AIDS Res Ther 7:31. 2010
    ..It is our view that the results described in this work suggest a novel approach to specifically promote death of HIV-1 infected cells and thus may eventually be developed into a new and general anti-viral therapy...
  2. pmc Transportin 3 and importin α are required for effective nuclear import of HIV-1 integrase in virus-infected cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Nucleus 1:422-31. 2010
    ....
  3. doi request reprint Mechanism of action of the HIV-1 integrase inhibitory peptide LEDGF 361-370
    Zvi Hayouka
    Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Biochem Biophys Res Commun 394:260-5. 2010
    ..We conclude that the full LEDGF 361-370 peptide is a potent HIV-1 inhibitor and may be used for further development as an anti-HIV lead compound...
  4. pmc Inhibiting HIV-1 integrase by shifting its oligomerization equilibrium
    Zvi Hayouka
    Department of Organic Chemistry, Department of Biological Chemistry, Protein Purification Unit, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 104:8316-21. 2007
    ..These peptides are promising anti-HIV lead compounds that modulate oligomerization of IN via a previously uncharacterized mechanism, which bears advantages over the conventional interface dimerization inhibitors...
  5. ncbi request reprint Stimulation of the HIV-1 integrase enzymatic activity and cDNA integration by a peptide derived from the integrase protein
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biopolymers 93:740-51. 2010
    ..These results suggest that the stimulatory effect of INS in wild-type virus-infected cells is due to a dual effect: it dissociates the inactive Rev-IN complex and directly activates the free IN...
  6. doi request reprint Correlation between shiftide activity and HIV-1 integrase inhibition by a peptide selected from a combinatorial library
    Ayelet Armon-Omer
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Mol Biol 376:971-82. 2008
    ..Thus, the results show a distinct correlation between the ability of the selected peptides to inhibit IN activity and that to shift its oligomerization equilibrium...
  7. doi request reprint Peptides that bind the HIV-1 integrase and modulate its enzymatic activity--kinetic studies and mode of action
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    FEBS J 278:316-30. 2011
    ..The IN-stimulating peptide bound IN in several specific sites that did not bind any of the inhibitory peptides. This may account for its unique activity...
  8. pmc Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Virol J 7:177. 2010
    ..In the absence of LEDGF/p75 very little integration and virus production can be detected, as was demonstrated using LEDGF/p75-knockdown cells...
  9. pmc Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteins
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Mol Med 16:34-44. 2010
    ..Our findings demonstrate that integration of HIV-1 cDNA is regulated by an interplay between viral Rev and the host-cell LEDGF/p75 proteins...
  10. ncbi request reprint Peptides derived from HIV-1 Rev inhibit HIV-1 integrase in a shiftide mechanism
    Zvi Hayouka
    Institute of Chemistry, Department of Biological Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Biopolymers 90:481-7. 2008
    ..We conclude that protein-protein interactions of IN may serve as a general valuable source for shiftide design...
  11. pmc Inhibition of HIV-1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Retrovirology 6:112. 2009
    ..The exact mechanisms underlying the nuclear import of IN and especially the question of whether it bears a functional nuclear localization signal (NLS) remain controversial...
  12. doi request reprint Peptide inhibitors of HIV-1 integrase: from mechanistic studies to improved lead compounds
    Michal Maes
    Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Bioorg Med Chem 17:7635-42. 2009
    ..Mapping the peptide binding sites in IN provided a model that explains this difference. We conclude that IN1 1-10 is an improved lead compound for further development of IN inhibitors...
  13. doi request reprint Cyclic peptide inhibitors of HIV-1 integrase derived from the LEDGF/p75 protein
    Zvi Hayouka
    Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Israel
    Bioorg Med Chem 18:8388-95. 2010
    ..The NMR structure of c(MZ 4-1) showed that it obtains a bioactive conformation that is similar to the parent site in LEDGF/p75...
  14. doi request reprint A novel role for the viral Rev protein in promoting resistance to superinfection by human immunodeficiency virus type 1
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Gen Virol 91:1503-13. 2010
    ..The involvement of Rev, expressed from either unintegrated or integrated viral cDNA, in promoting SIR was clearly demonstrated. We conclude that SIR involves Rev-dependent control of HIV cDNA integration...
  15. pmc Peptides derived from HIV-1 integrase that bind Rev stimulate viral genome integration
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel
    PLoS ONE 4:e4155. 2009
    ..This ordered sequence of events, centered on integration, is mandatory for HIV replication...
  16. doi request reprint A comparative study of backbone versus side chain peptide cyclization: application for HIV-1 integrase inhibitors
    Zvi Hayouka
    Institute of Chemistry, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Bioorg Med Chem 20:3317-22. 2012
    ..Due to the high costs and efforts involved in preparing BC peptides, SC may be a more approachable method in many cases. We suggest that both methods are interchangeable...
  17. pmc Novel regulation of HIV-1 replication and pathogenicity: Rev inhibition of integration
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Protein Eng Des Sel 22:753-63. 2009
    ..Our results thus demonstrate a new regulatory function of Rev and probably establish a link between Rev restriction of HIV-1 integration and protection of HIV-1-infected cells from premature cell death...
  18. ncbi request reprint Permeabilized mammalian cells as an experimental system for nuclear import of geminiviral karyophilic proteins and of synthetic peptides derived from their nuclear localization signal regions
    Gideon Kass
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Gen Virol 87:2709-20. 2006
    ..BV1-NLS and CP-NLS were shown to mediate specific binding to importin alpha, both in vitro and in vivo. These results are consistent with a common nuclear-import pathway for CP and BV1, probably via importin alpha...
  19. doi request reprint Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Virus Genes 40:341-6. 2010
    ..Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells' death...
  20. ncbi request reprint Functional analysis of backbone cyclic peptides bearing the arm domain of the HIV-1 Rev protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expression
    Elana Hariton-Gazal
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9104, Israel
    Biochemistry 44:11555-66. 2005
    ..The potential of BC peptides bearing ARM domains as lead compounds for the production of anti-HIV drugs is discussed...
  21. ncbi request reprint Translocation of histone proteins across lipid bilayers and Mycoplasma membranes
    Joseph Rosenbluh
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem 91904, Jerusalem, Israel
    J Mol Biol 345:387-400. 2005
    ..Based on the present and previous data histones appear to be characterized by the same features that characterize cell penetrating peptides and proteins (CPPs)...
  22. ncbi request reprint A synthetic peptide bearing the HIV-1 integrase 161-173 amino acid residues mediates active nuclear import and binding to importin alpha: characterization of a functional nuclear localization signal
    Ayelet Armon-Omer
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Mol Biol 336:1117-28. 2004
    ..The NLS(IN) as well as the full-length IN protein interacted specifically with importin alpha, binding of which was blocked by the NLS(IN) peptide itself as well as by the NLS-SV40...
  23. pmc Strategies to inhibit viral protein nuclear import: HIV-1 as a target
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biochim Biophys Acta 1813:1646-53. 2011
    ..This article is part of a Special Issue entitled: Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import...
  24. doi request reprint Monitoring the HIV-1 integrase enzymatic activity using atomic force microscopy in a 2LTR system
    Shlomit Guy
    Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Chem Commun (Camb) 49:3113-5. 2013
    ..It is mediated by the viral integrase (IN) enzyme, which is an attractive anti-HIV drug target. Here we present the first AFM imaging of IN-mediated DNA integration products in a two-LTR system...
  25. ncbi request reprint Positively charged peptides can interact with each other, as revealed by solid phase binding assays
    Joseph Rosenbluh
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    Anal Biochem 352:157-68. 2006
    ..We discuss the relevance of our findings for the use of solid phase assay systems to study interactions between biomolecules...
  26. doi request reprint A structural model of the HIV-1 Rev-integrase complex: the molecular basis of integrase regulation by Rev
    Hadar Benyamini
    Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biochem Biophys Res Commun 416:252-7. 2011
    ..Our model is expected to contribute to the development of lead compounds that inhibit the Rev-IN interaction and thus lead to multi-integration of viral cDNA and consequently to apoptosis of HIV-1 infected cells...
  27. pmc The plant VirE2 interacting protein 1. a molecular link between the Agrobacterium T-complex and the host cell chromatin?
    Abraham Loyter
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Plant Physiol 138:1318-21. 2005
  28. ncbi request reprint Non-endocytic penetration of core histones into petunia protoplasts and cultured cells: a novel mechanism for the introduction of macromolecules into plant cells
    Joseph Rosenbluh
    Department of Biological Chemistry, The Alexander Silberman Institute of Life, Sciences, The Hebrew University of Jerusalem, 91904, Israel
    Biochim Biophys Acta 1664:230-40. 2004
    ..To the best of our knowledge, the findings of the present paper demonstrate, for the first time, the activity of cell penetrating proteins (CPPs) in plant cells...
  29. ncbi request reprint Interaction between HIV-1 Rev and integrase proteins: a basis for the development of anti-HIV peptides
    Joseph Rosenbluh
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, and Department of Organic Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
    J Biol Chem 282:15743-53. 2007
    ..The present work describes novel anti-HIV-1 lead peptides that inhibit viral replication in cultured cells by blocking DNA integration in vivo...
  30. ncbi request reprint Inhibition of nuclear import mediated by the Rev-arginine rich motif by RNA molecules
    Konstantin Fineberg
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochemistry 42:2625-33. 2003
    ..The implication of our results for the regulation of the nuclear import of Rev as well as for the use of RNA molecules as antiviral drugs is discussed...