Research Topics
| A GertlerSummaryAffiliation: The Hebrew University Country: Israel Publications
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Publications
Development of leptin antagonists and their potential use in experimental biology and medicineArieh Gertler
The Institute of Biochemistry, Food Science, and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
Trends Endocrinol Metab 17:372-8. 2006..The recent development of leptin muteins with antagonistic properties and other proteins that block leptin activity opens up new possibilities for their use in research and, eventually, therapy...- Fluorescence resonance energy transfer (FRET) microscopy in living cells as a novel tool for the study of cytokine actionArieh Gertler
The Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
J Dairy Res 72:14-9. 2005.... - Mechanism of ruminant placental lactogen action: molecular and in vivo studiesArieh Gertler
Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Mol Genet Metab 75:189-201. 2002.... - Large-scale preparation of biologically active recombinant ovine obese protein (leptin)A Gertler
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
FEBS Lett 422:137-40. 1998.... - Large-scale preparation of recombinant ovine prolactin and determination of its in vitro and in vivo activityH Leibovich
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Protein Expr Purif 22:489-96. 2001..In contrast to ovine growth hormone or ovine placental lactogen, recombinant oPRL had no galactopoietic effect in lactating ewes... 
Nonsynonymous natural genetic polymorphisms in the bovine leptin gene affect biochemical and biological characteristics of the mature hormoneS Reicher
The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
J Anim Sci 90:410-8. 2012..05) inhibitory effect than the WT LEP. It was concluded that natural genetic polymorphisms in the bLEP are associated with variation in the biochemical and physiological properties of the protein...- Mutations of ovine and bovine placental lactogens change, in different ways, the biological activity mediated through homologous and heterologous lactogenic receptorsD Helman
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
J Endocrinol 169:43-54. 2001.... - Pituitary and placental ovine growth hormone variants differ in their receptor-binding ability and in their biological propertiesS Reicher
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
Gen Comp Endocrinol 155:368-77. 2008..Thus the product of the oGH duplicated copy has higher affinity for GHR and higher somatogenic activity. As the GH2-Z gene copy is expressed in the placenta, allelic differences at the oGH locus may influence feto-placental development... - Extracellular domain of prolactin receptor from bovine mammary gland: expression in Escherichia coli, purification and characterization of its interaction with lactogenic hormonesA Tchelet
Department of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
J Endocrinol 144:393-403. 1995.... - Mitogenic and binding properties of monoclonal antibodies to the prolactin receptor in Nb2 rat lymphoma cells. Selective enhancement by anti-mouse IgGG Elberg
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
J Biol Chem 265:14770-6. 1990..It may be induced by binding of the mAb to a site, which can be either identical or may even be distinct from that which binds the lactogenic hormone... - Ovine placental lactogen-induced heterodimerization of ovine growth hormone and prolactin receptors in living cells is demonstrated by fluorescence resonance energy transfer microscopy and leads to prolonged phosphorylation of signal transducer and activaEva Biener
The Institute of Biochemistry, Food Science, and Nutrition, Faculty of Agricultural, and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Endocrinology 144:3532-40. 2003.... 
Mapping leptin-interacting sites in recombinant leptin-binding domain (LBD) subcloned from chicken leptin receptorL Niv-Spector
Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Biochem J 390:475-84. 2005..Contact-surface analysis identified the residues having the highest contribution to the recognition site to be Phe73, Phe77 and Leu79...- Large-scale preparation and in vitro characterization of biologically active human placental (20 and 22K) and pituitary (20K) growth hormones: placental growth hormones have no lactogenic activity in humansG Solomon
Institute of Biochemistry, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Growth Horm IGF Res 16:297-307. 2006..3-fold lower than that of hGH-N 22K. The latter finding raises the question of whether the lack of intrinsic lactogenic activity in the placental hGHs that dominate during pregnancy has any physiological relevance... - Absorption of bioactive human growth hormone after oral administration in the common carp (Cyprinus carpio) and its enhancement by deoxycholateY Hertz
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
J Comp Physiol B 161:159-63. 1991..Additional studies are required to establish the appropriate conditions (diet composition, feeding level, and frequency, etc.) in which polypeptide hormones could be introduced orally to fish... - Transactivation of erbB2 by short and long isoforms of leptin receptorsAvital Eisenberg
Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
FEBS Lett 565:139-42. 2004..Using FRET technology in living cells, we found no evidence of complex formation between erbB2 and prolactin or leptin receptors, indicating that the transactivation occurs through an indirect interaction... - Oral immunization of Carassius auratus with modified recombinant A-layer proteins entrapped in alginate beadsSarah Maurice
Institute of Biochemistry, The Hebrew University of Jerusalem, P O Box 12, Rechovot 76100, Israel
Vaccine 23:450-9. 2004..In spite of the presence of an increased titer to A-protein, vaccinated fish did not demonstrate resistance to infection with atypical A. salmonicida... - Purification and characterization of recombinant pufferfish (Takifugu rubripes) leptinM Yacobovitz
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
Gen Comp Endocrinol 156:83-90. 2008..No specific binding of either ovine or pufferfish leptins to tilapia liver membranes was detected. This work is the first report on the purification of leptin from any fish species... - Spatio-temporal kinetics of growth hormone receptor signaling in single cells using FRET microscopyEva Biener-Ramanujan
The Institute of Biochemistry, Food Science, and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Growth Horm IGF Res 16:247-57. 2006..Although GHR dimerization and STAT5 activation have been reported previously, this is the first spatially resolved demonstration of GHR-signaling kinetics in intact cells... - Identification of the hydrophobic strand in the A-B loop of leptin as major binding site III: implications for large-scale preparation of potent recombinant human and ovine leptin antagonistsLeonora Niv-Spector
Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, Rehovot 76100, Israel
Biochem J 391:221-30. 2005.... - Bacterial expression of prolactin family proteinsArieh Gertler
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Israel
Methods Mol Med 122:341-54. 2006..The methodology described in the present chapter allows large-scale preparation of pure, monomeric, biologically active oPL, oPRL, and rbPRLR-ECD suitable for performing in vitro and in vivo experiments... - Subcloning, expression, purification, and characterization of recombinant human leptin-binding domainYael Sandowski
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, Rehovot 76100, Israel
J Biol Chem 277:46304-9. 2002..human leptin complex. Two main residues, Phe-500, located in loop L3, and Tyr-441, located in L1, are suggested to contribute to leptin binding... - Cellulose beads bound to cellulose binding domain-fused recombinant proteins; an adjuvant system for parenteral vaccination of fishSarah Maurice
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Freiburg Building, Rm 10, P O Box 12, Rehovot, 76100, Israel
Vaccine 21:3200-7. 2003..The paper describes a simple and safe method to increase the potential immunogenicity of soluble recombinant proteins by employing relatively inexpensive cellulose particles... - Preparation and expression of biologically active prolactin and growth hormone receptors and suppressor of cytokine signaling proteins 1, 2, 3, and 6 tagged with cyan and yellow fluorescent proteinsLiora Ben-Yair
Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, Institute of Biochemistry, The Hebrew University, Rehovot 76100, Israel
Protein Expr Purif 25:456-64. 2002..Cells transfected with eight of these tagged constructs expressed the fluorescent proteins in both the nucleus and cytosol; the tagged SOCS2 was localized mostly in the latter compartment... - Regulation of heat-shock protein (hsp70) gene expression by hGH and IL2 in rat Nb2 lymphoma cellsS Horn
Department of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel
Mol Cell Endocrinol 105:139-46. 1994..These results indicate that activation of PKC is one of the signaling pathways differentially involved in hGH and IL2 stimulation of cells.(ABSTRACT TRUNCATED AT 250 WORDS).. - Comparison of R128Q mutations in human, ovine, and chicken leptinsNina Raver
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Gen Comp Endocrinol 126:52-8. 2002..This made it impossible to correlate the results shown in the present work with the currently available models. Therefore, leptin may bind its receptors in a manner different than those proposed until now... - Recombinant human CIS2 (SOCS2) protein: subcloning, expression, purification, and characterizationEva Biener
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
Protein Expr Purif 25:305-12. 2002..Recombinant CIS2 was compared by biochemical, immunological, and molecular methods to the CIS2 protein expressed in eukaryotic cells. This report describes the first substantial production of biologically active recombinant human CIS2... - Quantitative FRET imaging of leptin receptor oligomerization kinetics in single cellsEva Biener
The Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
Biol Cell 97:905-19. 2005.... - Preparation of leptin antagonists by site-directed mutagenesis of human, ovine, rat, and mouse leptin's site III: implications on blocking undesired leptin action in vivoGili Solomon
Faculty of Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
Ann N Y Acad Sci 1091:531-9. 2006..Some muteins were pegylated using 40 kDa PEG. Although pegylation decreased the in vitro activity, increasing circulation half-life can recompensate this deficit, so pegylated antagonists are expected to be more potent in vivo... - Large-scale preparation of biologically active mouse and rat leptins and their L39A/D40A/F41A muteins which act as potent antagonistsGili Salomon
Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, Rehovot 76100, Israel
Protein Expr Purif 47:128-36. 2006.... - A simple novel method for the preparation of noncovalent homodimeric, biologically active human interleukin 10 in Escherichia coli-enhancing protein expression by degenerate PCR of 5' DNA in the open reading frameShelley Klompus
Faculty of Agricultural, Food and Environmental Quality Sciences, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot, Israel
Protein Expr Purif 62:199-205. 2008..The yield of recombinant hIL-10 from 10L of fermentation culture was 60mg and a protocol for its long-term storage as a carrier-free lyophilized powder at -20 degrees was developed... - The obese phenotype-inducing N82K mutation in human leptin disrupts receptor-binding and biological activityLeonora Niv-Spector
Institute of Biochemistry, Food Science and Nutrition, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Mol Genet Metab 100:193-7. 2010.... - Ovine placental lactogen specifically binds to endometrial glands of the ovine uterusSekoni Noel
Center for Animal Biotechnology and Genomics and Department of Animal Science, Texas A and M University, College Station, Texas 77843-2471, USA
Biol Reprod 68:772-80. 2003..Thus, effects of oPL on the endometrial glands may be mediated by receptors for oPRL and oGH... - Suppressor of cytokine signaling 7 inhibits prolactin, growth hormone, and leptin signaling by interacting with STAT5 or STAT3 and attenuating their nuclear translocationNele Martens
Neuroendocrine Immunology, and Pharmacology Department, Medical School, Free University, B 1090 Brussels, Belgium
J Biol Chem 280:13817-23. 2005.... - The effect of neonatal leptin treatment on postnatal weight gain in male rats is dependent on maternal nutritional status during pregnancyMark H Vickers
Liggins Institute, University of Auckland, Auckland, New Zealand
Endocrinology 149:1906-13. 2008..These data further reinforce the importance of leptin in determining long-term energy homeostasis, and suggest that leptin's effects are modulated by gender and both prenatal and postnatal nutritional status... - Long-term consequences of maternal high-fat feeding on hypothalamic leptin sensitivity and diet-induced obesity in the offspringJacqueline Férézou-Viala
Neurobiologie de l Olfaction et de la Prise Alimentaire, UMR 1197, Bâtiment 447, Universite Paris Sud, F 91 405 Orsay Cedex, France
Am J Physiol Regul Integr Comp Physiol 293:R1056-62. 2007..We conclude that maternal high-fat diet programs a hypothalamic leptin resistance in offspring, which, however, fails to increase the body weight gain until adulthood... - Leptin modulates the expression of secreted and membrane-associated mucins in colonic epithelial cells by targeting PKC, PI3K, and MAPK pathwaysMahmoud El Homsi
INSERM UMR865, Faculté de Médecine R Laennec, 7 rue Guillaume Paradin, 69008 Lyon, France
Am J Physiol Gastrointest Liver Physiol 293:G365-73. 2007..These data are consistent with a role for leptin in regulation of the intestinal barrier function... - Leptin downregulates heat shock protein-70 (HSP-70) gene expression in chicken liver and hypothalamusDenise Figueiredo
Laboratory of Physiology, Immunology and Genetics of Domestic Animals, Catholic University of Leuven, 3001 Heverlee, Belgium
Cell Tissue Res 329:91-101. 2007..Furthermore, HSP-70 gene expression was gender-dependent with significantly (P < 0.05) higher levels in male than in female chickens... - Preparation and characterization of recombinant chicken growth hormone (chGH) and its putative antagonist chGH G119R muteinHelena E Paczoska-Eliasiewicz
Department of Animal Physiology, University of Agriculture, Al. Mickiewicza 24/28, , Poland
Ann N Y Acad Sci 1091:501-8. 2006..These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chGH G119R... - The regulation of stearoyl-CoA desaturase gene expression is tissue specific in chickensSami Dridi
Laboratory of Physiology, Immunology, and Genetics of Domestic Animals, Catholic University of Leuven, KU Leuven, 3001 Heverlee, Belgium
J Endocrinol 192:229-36. 2007..05). In conclusion, SCD is ubiquitously expressed in chickens and it is regulated by leptin, cerulenin, nutritional state, and gender in a tissue-specific manner... - Synergistic action of growth hormone and insulin-like growth factor I (IGF-I) on proliferation and estradiol secretion in porcine granulosa and theca cells cultured alone or in cocultureJolanta Kolodziejczyk
Laboratory of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Ingardena 6, , Poland
Theriogenology 60:559-70. 2003..The promotion of the mitogenic activity in Tc by IGF-I during all stages of follicular development suggests an important role for theca cells in follicular growth... - Divergent effects of leptin on luteinizing hormone and insulin secretion are dose dependentDorota A Zieba
Animal Reproduction Laboratory, Texas A and M University Agricultural Research Station, Beeville, Texas 78102-9410, USA
Exp Biol Med (Maywood) 228:325-30. 2003..Collectively, our results show that leptin has potent positive effects on both LH and insulin secretion in fasted cows, but the anterior pituitary and endocrine pancreas appear to become downregulated in the presence of excess ligand... - Exogenous leptin advances puberty in domestic henHelena Elzbieta Paczoska-Eliasiewicz
Department of Animal Physiology, University of Agriculture, Krakow, Poland
Domest Anim Endocrinol 31:211-26. 2006..In conclusion, we have shown that in female chickens exogenous leptin advances the onset of puberty by attenuation of ovarian apoptosis and enhancement of folliculogenesis... - Insulin and leptin induce Glut4 plasma membrane translocation and glucose uptake in a human neuronal cell line by a phosphatidylinositol 3-kinase- dependent mechanismYacir Benomar
Neuroendocrinologie Moléculaire de la Prise Alimentaire, Neurobiologie de l Olfaction et de la Prise Alimentaire, Institut National de la Recherche Agronomique, Universite Paris XI, Orsay, France
Endocrinology 147:2550-6. 2006..These pathways might contribute to an in vivo glucoregulatory reflex involving a neuronal network and to the anorectic effect of insulin and leptin... - Early signaling interactions between the insulin and leptin pathways in bovine myogenic cellsA Lulu Strat
Department of Animal and Veterinary Science, University of Idaho, Moscow, 8344, USA
Biochim Biophys Acta 1744:164-75. 2005.... - Prenatal influences on leptin sensitivity and susceptibility to diet-induced obesityStefan O Krechowec
Liggins Institute, The University of Auckland, Private Bag 92019, Auckland, New Zealand
J Endocrinol 189:355-63. 2006..Our study suggests that prenatal nutrition can shape future susceptibility to obesity through alterations in leptin sensitivity and changes in energy metabolism during adult life... - Prolactin regulation of neonatal ovine uterine gland morphogenesisKaren D Carpenter
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A and M University, College Station, Texas 77843-2471, USA
Endocrinology 144:110-20. 2003..Collectively, results indicate that PRL regulates endometrial adenogenesis in the neonatal ovine uterus... - The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexinNele Martens
Neuroendocrine Immunology Group, Pharmacology Department, Medical School, Free University of Brussels VUB, 1090, Belgium
Exp Cell Res 298:239-48. 2004..Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton... - Is leptin an important physiological regulator of CRP?Arieh Gertler
Nat Med 13:18-9; author reply 19-21. 2007