Hanna Engelberg Kulka

Summary

Affiliation: The Hebrew University
Country: Israel

Publications

  1. pmc Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions
    Ronen Hazan
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Bacteriol 186:3663-9. 2004
  2. pmc MazF-mediated cell death in Escherichia coli: a point of no return
    Shahar Amitai
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Bacteriol 186:8295-300. 2004
  3. ncbi request reprint mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Cell Sci 118:4327-32. 2005
  4. pmc Induction of Escherichia coli chromosomal mazEF by stressful conditions causes an irreversible loss of viability
    Ilana Kolodkin-Gal
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Bacteriol 188:3420-3. 2006
  5. pmc Bacterial programmed cell death and multicellular behavior in bacteria
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Genet 2:e135. 2006
  6. pmc A differential effect of E. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation
    Ilana Kolodkin-Gal
    Department of Molecular Biology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
    PLoS ONE 4:e6785. 2009
  7. pmc Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins"
    Shahar Amitai
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Genet 5:e1000390. 2009
  8. pmc Novel quorum-sensing peptides mediating interspecies bacterial cell death
    Sathish Kumar
    Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    MBio 4:e00314-13. 2013
  9. pmc Two programmed cell death systems in Escherichia coli: an apoptotic-like death is inhibited by the mazEF-mediated death pathway
    Ariel Erental
    Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel Canada, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Biol 10:e1001281. 2012
  10. ncbi request reprint Bacterial programmed cell death systems as targets for antibiotics
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Trends Microbiol 12:66-71. 2004

Research Grants

  1. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2010
  2. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2007
  3. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2009

Collaborators

  • C Li
  • Jurij Lah
  • R Loris
  • Hanna Engelberg-Kulka
  • Ilana Kolodkin-Gal
  • Shahar Amitai
  • Ronen Hazan
  • Boaz Sat
  • H Engelberg-Kulka
  • Sathish Kumar
  • Ariel Erental
  • Myriam Reches
  • Idith Sharon
  • M Reches
  • Mirit Hananya-Meltabashi
  • Reut Verdiger
  • Ayelet Sacher
  • Ayalla Shlosberg-Fedida
  • Alex Keshet
  • G Glaser
  • Ariel Gaathon
  • Shmuel Carmeli
  • Yussuf Yassin
  • Z Liu
  • Y Klemes
  • S Narasimhan
  • E Aizenman
  • R Schoulaker-Schwarz

Detail Information

Publications22

  1. pmc Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions
    Ronen Hazan
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Bacteriol 186:3663-9. 2004
    ..We also show that this process takes place only during logarithmic growth and requires an intact relA gene...
  2. pmc MazF-mediated cell death in Escherichia coli: a point of no return
    Shahar Amitai
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Bacteriol 186:8295-300. 2004
    ..Our results reported here support our view that the mazEF module mediates cell death and is part of a programmed cell death network...
  3. ncbi request reprint mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Cell Sci 118:4327-32. 2005
    ..The mazEF-mediated death pathway can act as a defense mechanism that prevents the spread of bacterial phage infection, allowing bacterial populations to behave like multicellular organisms...
  4. pmc Induction of Escherichia coli chromosomal mazEF by stressful conditions causes an irreversible loss of viability
    Ilana Kolodkin-Gal
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Bacteriol 188:3420-3. 2006
    ..These results further support our previous conclusion that E. coli mazEF mediation of cell death is not a passive process, but an active and genetically "programmed" death response...
  5. pmc Bacterial programmed cell death and multicellular behavior in bacteria
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Genet 2:e135. 2006
    ..We relate these two bacterial PCD systems to the ways in which bacterial populations resemble multicellular organisms...
  6. pmc A differential effect of E. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation
    Ilana Kolodkin-Gal
    Department of Molecular Biology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
    PLoS ONE 4:e6785. 2009
    ..Cell death governed by mazEF and dinJ-YafQ seems to participate in biofilm formation through a novel mechanism...
  7. pmc Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins"
    Shahar Amitai
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Genet 5:e1000390. 2009
    ....
  8. pmc Novel quorum-sensing peptides mediating interspecies bacterial cell death
    Sathish Kumar
    Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    MBio 4:e00314-13. 2013
    ..Furthermore, each of these peptides provides the basis of a new class of antibiotics triggering death by acting from outside the cell...
  9. pmc Two programmed cell death systems in Escherichia coli: an apoptotic-like death is inhibited by the mazEF-mediated death pathway
    Ariel Erental
    Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel Canada, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Biol 10:e1001281. 2012
    ..Based on these results, we offer a molecular model for the maintenance of an altruistic characteristic in cell populations. In our model, the ALD pathway is inhibited by the altruistic EDF-mazEF-mediated death pathway...
  10. ncbi request reprint Bacterial programmed cell death systems as targets for antibiotics
    Hanna Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Trends Microbiol 12:66-71. 2004
    ..Because multi-drug resistance among bacterial pathogens is becoming more widespread, the results obtained might be useful as a basis for producing alternative drugs...
  11. ncbi request reprint Microbiology. Cannibals defy starvation and avoid sporulation
    Hanna Engelberg-Kulka
    Department of Molecular Biology, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Science 301:467-8. 2003
  12. ncbi request reprint Addiction modules and programmed cell death and antideath in bacterial cultures
    H Engelberg-Kulka
    Department of Molecular Biology, Hebrew University Hadassah Medical School, Jerusalem, Israel
    Annu Rev Microbiol 53:43-70. 1999
    ..Finally, we discuss the similarities between basic features of programmed cell death and antideath in both prokaryotes and eukaryotes and the possibility that they share a common evolutionary origin...
  13. ncbi request reprint An extended Escherichia coli "selenocysteine insertion sequence" (SECIS) as a multifunctional RNA structure
    H Engelberg-Kulka
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Biofactors 14:61-8. 2001
    ..Depending on the availability of selenium the SECIS enables one of two alternatives for the translational machinery: Either selenocysteine incorporation into a polypeptide or termination of the polypeptide chain...
  14. pmc rexB of bacteriophage lambda is an anti-cell death gene
    H Engelberg-Kulka
    Department of Molecular Biology, Hebrew University Hadassah Medical School, Jerusalem 91120 Israel
    Proc Natl Acad Sci U S A 95:15481-6. 1998
    ..Thus, the rex operon may be considered as the "survival operon" of phage lambda...
  15. pmc The Escherichia coli mazEF suicide module mediates thymineless death
    Boaz Sat
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Bacteriol 185:1803-7. 2003
    ..Our results, which describe thymine starvation as a trigger for a built-in death program, have implications for programmed cell death in both prokaryotes and eukaryotes...
  16. pmc The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics
    Ilana Kolodkin-Gal
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS Biol 6:e319. 2008
    ..Furthermore, our results showed that the mode of action of antibiotics was determined by the ability of E. coli cells to communicate through the signaling molecule Extracellular Death Factor (EDF) participating in mazEF induction...
  17. pmc The stationary-phase sigma factor sigma(S) is responsible for the resistance of Escherichia coli stationary-phase cells to mazEF-mediated cell death
    Ilana Kolodkin-Gal
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Bacteriol 191:3177-82. 2009
    ..Deletion of rpoS, the gene encoding sigma(S) from the bacterial chromosome, permitted mazEF-mediated cell death during stationary growth phase...
  18. ncbi request reprint A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli
    Ilana Kolodkin-Gal
    Department of Molecular Biology, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Science 318:652-5. 2007
    ..Structural analysis revealed that EDF is a linear pentapeptide, Asn-Asn-Trp-Asn-Asn. Each of the five amino acids of EDF is important for its activity...
  19. pmc The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli
    Ilana Kolodkin-Gal
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Bacteriol 190:3169-75. 2008
    ..Significant strain differences in EDF production and response explain variations in the induction of mazEF-mediated cell death...
  20. ncbi request reprint Recognition of the intrinsically flexible addiction antidote MazE by a dromedary single domain antibody fragment. Structure, thermodynamics of binding, stability, and influence on interactions with DNA
    Jurij Lah
    Department of Ultrastructure, Vrije Universiteit Brussel, Paardenstraat 65, B 1640 St Genesius Rode, Belgium
    J Biol Chem 278:14101-11. 2003
    ..Both binding and stability studies lead to a picture of MazE solution structure that is significantly more unfolded than the structure observed in a crystal of the MazE-cAbMaz1 complex...
  21. ncbi request reprint Energetics of structural transitions of the addiction antitoxin MazE: is a programmed bacterial cell death dependent on the intrinsically flexible nature of the antitoxins?
    Jurij Lah
    University of Ljubljana, Faculty of Chemistry and Chemical Technology, Askerceva 5, 1000 Ljubljana, Slovenia
    J Biol Chem 280:17397-407. 2005
    ....
  22. ncbi request reprint Crystal structure of the intrinsically flexible addiction antidote MazE
    Remy Loris
    Laboratorium voor Ultrastructuur en Vlaams instituut voor Biotechnologie, Vrije Universiteit Brussel, Gebouw E, Pleinlaan 2, 1050 Brussel, Belgium
    J Biol Chem 278:28252-7. 2003
    ..This hydrophobic core consists exclusively of short aliphatic residues. The folded part of MazE contains a novel DNA binding motif. A model for DNA binding that is consistent with the available biochemical data is presented...

Research Grants4

  1. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2010
    ..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..
  2. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2007
    ..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..
  3. mazEF-mediated programmed cell death network in E. coli
    Hanna Engelberg Kulka; Fiscal Year: 2009
    ..Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem. ..