Amnon Hizi

Summary

Affiliation: Tel Aviv University
Country: Israel

Publications

  1. pmc Inhibition of the integrases of human immunodeficiency viruses type 1 and type 2 by reverse transcriptases
    Iris Oz
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Biochem J 361:557-66. 2002
  2. ncbi request reprint The integrase of the long terminal repeat-retrotransposon tf1 has a chromodomain that modulates integrase activities
    Amnon Hizi
    Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:39086-94. 2005
  3. pmc The reverse transcriptase of the Tf1 retrotransposon has a specific novel activity for generating the RNA self-primer that is functional in cDNA synthesis
    Amnon Hizi
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Virol 82:10906-10. 2008
  4. doi request reprint Retroviral reverse transcriptases (other than those of HIV-1 and murine leukemia virus): a comparison of their molecular and biochemical properties
    Amnon Hizi
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Virus Res 134:203-20. 2008
  5. ncbi request reprint Inhibition of the activities of reverse transcriptase and integrase of human immunodeficiency virus type-1 by peptides derived from the homologous viral protein R (Vpr)
    Iris Oz Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    J Mol Biol 369:1230-43. 2007
  6. ncbi request reprint Inhibition of human immunodeficiency virus type-1 reverse transcriptase by a novel peptide derived from the viral integrase
    Iris Oz Gleenberg
    Department of Cell and Developmental Biology, The Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Arch Biochem Biophys 458:202-12. 2007
  7. ncbi request reprint De novo parallel design, synthesis and evaluation of inhibitors against the reverse transcriptase of human immunodeficiency virus type-1 and drug-resistant variants
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Med Chem 50:2370-84. 2007
  8. ncbi request reprint Expression and characterization of a recombinant novel reverse transcriptase of a porcine endogenous retrovirus
    Orna Avidan
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Israel
    Virology 307:341-57. 2003
  9. ncbi request reprint Expression and characterization of the integrase of bovine immunodeficiency virus
    Orna Avidan
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Virology 371:309-21. 2008
  10. doi request reprint Quantitative analysis of the interactions between HIV-1 integrase and retroviral reverse transcriptases
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochem J 412:163-70. 2008

Collaborators

  • Arie Admon
  • Michal Entin-Meer
  • Neora Yaal-Hahoshen
  • Zvi Hayouka
  • Stuart F J Le Grice
  • Noam Adir
  • Assaf Friedler
  • H L Levin
  • Alon Herschhorn
  • Iris Oz-Gleenberg
  • Iris Oz Gleenberg
  • Orna Avidan
  • Eytan Herzig
  • Shoshana Loya
  • Yehuda Goldgur
  • Moria Shimoni
  • Nickolay Voronin
  • Ziv Sevilya
  • Iris Oz
  • Maya Golan
  • Noa Kirshenboim
  • Klara Post
  • Yelena Britan-Rosich
  • Moshe Kotler
  • Itai Benhar
  • Wayne A Marasco
  • Iafa Keydar
  • Hadar Reichman
  • Ilan Tsarfaty
  • James H Resau
  • Michal Weitman
  • Lena Lerman
  • Abraham Nudelman
  • Ron Bochner
  • Ralf R Tönjes
  • Jianhui Guo
  • Judith G Levin
  • Amit Duvshani
  • Jennifer T Miller
  • Kathryn J Howard
  • Michael D Powell
  • Amira Rudi
  • Yoel Kashman
  • Michal Entin Meer

Detail Information

Publications33

  1. pmc Inhibition of the integrases of human immunodeficiency viruses type 1 and type 2 by reverse transcriptases
    Iris Oz
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Biochem J 361:557-66. 2002
    ..We postulate that sequences and/or three-dimensional protein structures common to RTs interact with INs and inhibit their activities. We show evidence for this hypothesis and discuss the possible sites of IN involved in this interaction...
  2. ncbi request reprint The integrase of the long terminal repeat-retrotransposon tf1 has a chromodomain that modulates integrase activities
    Amnon Hizi
    Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:39086-94. 2005
    ..These results reveal that both the activity and specificity of enzymes can be modulated by their chromodomains...
  3. pmc The reverse transcriptase of the Tf1 retrotransposon has a specific novel activity for generating the RNA self-primer that is functional in cDNA synthesis
    Amnon Hizi
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Virol 82:10906-10. 2008
    ..In all, it is likely that the observed unique mechanism of self-priming in Tf1 represents an early advantageous form of initiating reverse transcription in LTR retroelements without involving cellular tRNAs...
  4. doi request reprint Retroviral reverse transcriptases (other than those of HIV-1 and murine leukemia virus): a comparison of their molecular and biochemical properties
    Amnon Hizi
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Virus Res 134:203-20. 2008
    ..The comparisons between the diverse properties of the various RTs show the common traits that characterize the RTs from all retroviral subfamilies. In addition, the unique features of the specific RTs groups are also discussed...
  5. ncbi request reprint Inhibition of the activities of reverse transcriptase and integrase of human immunodeficiency virus type-1 by peptides derived from the homologous viral protein R (Vpr)
    Iris Oz Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    J Mol Biol 369:1230-43. 2007
    ....
  6. ncbi request reprint Inhibition of human immunodeficiency virus type-1 reverse transcriptase by a novel peptide derived from the viral integrase
    Iris Oz Gleenberg
    Department of Cell and Developmental Biology, The Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Arch Biochem Biophys 458:202-12. 2007
    ..These results can facilitate the development of novel and specific peptide-based HIV-1 RT inhibitors that might help in the fight against AIDS...
  7. ncbi request reprint De novo parallel design, synthesis and evaluation of inhibitors against the reverse transcriptase of human immunodeficiency virus type-1 and drug-resistant variants
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Med Chem 50:2370-84. 2007
    ..Accordingly, 5f can serve as lead for developing novel inhibitors against RT that may be used to suppress HIV-1 growth...
  8. ncbi request reprint Expression and characterization of a recombinant novel reverse transcriptase of a porcine endogenous retrovirus
    Orna Avidan
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Israel
    Virology 307:341-57. 2003
    ..Therefore, the availability of large amounts of the recombinant RT can be useful for a wide screening of novel drugs against infectious PERV...
  9. ncbi request reprint Expression and characterization of the integrase of bovine immunodeficiency virus
    Orna Avidan
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Virology 371:309-21. 2008
    ....
  10. doi request reprint Quantitative analysis of the interactions between HIV-1 integrase and retroviral reverse transcriptases
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochem J 412:163-70. 2008
    ..These modes of direct protein interactions, along with the apparent rate constants calculated and the correlations of the interaction kinetics with the capacity of the RTs to inhibit IN activities, are all discussed...
  11. ncbi request reprint Mutagenesis of cysteine 280 of the reverse transcriptase of human immunodeficiency virus type-1: the effects on the ribonuclease H activity
    Ziv Sevilya
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Mol Biol 327:19-30. 2003
    ..This suggests that the modifications of C280 lead to an impaired affinity of HIV-1 RT towards the RNA-DNA substrate...
  12. doi request reprint Retroviral reverse transcriptases
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel
    Cell Mol Life Sci 67:2717-47. 2010
    ..These include, among other topics, their structures, enzymatic activities, interactions with both viral and host proteins, RT inhibition and resistance to antiretroviral drugs...
  13. ncbi request reprint Peptides derived from the reverse transcriptase of human immunodeficiency virus type 1 as novel inhibitors of the viral integrase
    Iris Oz Gleenberg
    Department of Cell and Developmental Biology, The Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 280:21987-96. 2005
    ..This information can substantially facilitate the development of novel drugs against HIV INs and thus contribute to the fight against AIDS...
  14. ncbi request reprint Expression and characterization of a novel reverse transcriptase of the LTR retrotransposon Tf1
    Noa Kirshenboim
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Virology 366:263-76. 2007
    ..In all, Tf1 RT has a marked infidelity in synthesizing DNA at template ends, a phenomenon that can explain, as discussed herein, some of the features of Tf1 replication in the host cells...
  15. doi request reprint Substrate variations that affect the nucleic acid clamp activity of reverse transcriptases
    Iris Oz-Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    FEBS J 279:1894-903. 2012
    ..These differences shed light on the dissimilarities in catalytic activities of RTs, and suggest that clamp activity may be a potential new target for anti-retroviral drugs...
  16. pmc The removal of RNA primers from DNA synthesized by the reverse transcriptase of the retrotransposon Tf1 is stimulated by Tf1 integrase
    Eytan Herzig
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    J Virol 86:6222-30. 2012
    ..These results highlight possible new functions of Tf1 integrase in the retrotransposon's reverse transcription process...
  17. doi request reprint Template-independent DNA synthesis activity associated with the reverse transcriptase of the long terminal repeat retrotransposon Tf1
    Iris Oz-Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    FEBS J 279:142-53. 2012
    ..The unusually high NTA activity of Tf1 RT can explain why, after completing cDNA synthesis, the in vivo generated Tf1 cDNA has relatively long extra sequences beyond the highly conserved CA at its 3'-ends...
  18. doi request reprint Virtual screening, identification, and biochemical characterization of novel inhibitors of the reverse transcriptase of human immunodeficiency virus type-1
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Med Chem 51:5702-13. 2008
    ..These inhibitors may serve as promising anti-HIV lead compounds...
  19. pmc Mode of inhibition of HIV-1 reverse transcriptase by polyacetylenetriol, a novel inhibitor of RNA- and DNA-directed DNA polymerases
    Shoshana Loya
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochem J 362:685-92. 2002
    ....
  20. ncbi request reprint The catalytic properties of the recombinant reverse transcriptase of bovine immunodeficiency virus
    Orna Avidan
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Virology 351:42-57. 2006
    ..The longer BIV RT version is somewhat less active than the shorter version, suggesting that the extra 74 residues (with homology to dUTPases) might obstruct efficient catalysis...
  21. pmc The role of phenylalanine-119 of the reverse transcriptase of mouse mammary tumour virus in DNA synthesis, ribose selection and drug resistance
    Michal Entin-Meer
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochem J 367:381-91. 2002
    ..Since the only three-dimensional structures of RTs published so far are those of HIV-1 and MLV, it is likely that MMTV RT folds quite similarly to these RTs...
  22. pmc The isolation of novel phage display-derived human recombinant antibodies against CCR5, the major co-receptor of HIV
    Moria Shimoni
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    Viral Immunol 26:277-90. 2013
    ..The experimental approach presented herein for screening for CCR5-specific antibodies can be applicable to screen antibody-presenting phage libraries against any cell-surface expressed protein of interest...
  23. pmc Human endogenous retrovirus (HERV-K) reverse transcriptase as a breast cancer prognostic marker
    Maya Golan
    Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    Neoplasia 10:521-33. 2008
    ..Furthermore, these results provide evidence for the possible involvement of endogenous retrovirus in human breast carcinoma...
  24. ncbi request reprint The mature reverse transcriptase molecules in virions of mouse mammary tumor virus possess protease-derived sequences
    Michal Entin-Meer
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Virology 310:157-62. 2003
    ....
  25. pmc Human immunodeficiency virus type 2 reverse transcriptase activity in model systems that mimic steps in reverse transcription
    Klara Post
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    J Virol 77:7623-34. 2003
    ..This may reflect architectural differences in the primer grip regions in the p66 (HIV-1) and p68 (HIV-2) palm subdomains of the two enzymes. The implications of our findings for antiviral therapy are discussed...
  26. pmc Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis
    Iris Oz-Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
    Nucleic Acids Res 39:1042-53. 2011
    ..These RT properties can shed light on potentially-new roles of RTs in the reverse-transcription process and define new targets for anti-retroviral drugs...
  27. ncbi request reprint Ile178 of HIV-1 reverse transcriptase is critical for inhibiting the viral integrase
    Iris Oz Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochem Biophys Res Commun 364:48-52. 2007
    ..Ile178 was found to be the major contributor to IN inhibition, probably by interacting with IN residue Gly149. As Gly149 is a key IN residue, this inhibition probably results from a steric hindrance of the IN active site...
  28. ncbi request reprint Mechanism of inhibition of HIV-1 reverse transcriptase by the novel broad-range DNA polymerase inhibitor N-{2-[4-(aminosulfonyl)phenyl]ethyl}-2-(2-thienyl)acetamide
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochemistry 47:490-502. 2008
    ....
  29. ncbi request reprint Recombinant human antibodies against the reverse transcriptase of human immunodeficiency virus type-1
    Alon Herschhorn
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Israel
    Biochim Biophys Acta 1648:154-63. 2003
    ..These results indicate that some of the effects of F-6 are mediated by the CDR3 of the heavy chain. The antibodies identified here will be further tested as intrabodies for their capacity to protect human cells from HIV-1 infection...
  30. ncbi request reprint The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus
    Orna Avidan
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    Eur J Biochem 269:859-67. 2002
    ..The pattern of mispair elongation by BLV RT suggests that the in vitro error proneness of BLV RT is closer to that of HIV-1 RT. These fidelity properties are discussed in the context of the various retroviral RTs studied so far...
  31. doi request reprint Strand selections resulting from the combined template-independent DNA synthesis and clamp activities of HIV-1 reverse transcriptase
    Iris Oz-Gleenberg
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Biochem Biophys Res Commun 408:482-8. 2011
    ....
  32. doi request reprint Antibodies and lentiviruses that specifically recognize a T cell epitope derived from HIV-1 Nef protein and presented by HLA-C
    Alon Herschhorn
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    J Immunol 185:7623-32. 2010
    ..Abs and pseudoviruses with such specificity are likely to be highly valuable as building blocks for specific targeting and killing of HIV-1-infected cells...
  33. pmc Inhibiting HIV-1 integrase by shifting its oligomerization equilibrium
    Zvi Hayouka
    Department of Organic Chemistry, Department of Biological Chemistry, Protein Purification Unit, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 104:8316-21. 2007
    ..These peptides are promising anti-HIV lead compounds that modulate oligomerization of IN via a previously uncharacterized mechanism, which bears advantages over the conventional interface dimerization inhibitors...