Affiliation: Tel Aviv University
Shayevitch R, Askayo D, Keydar I, Ast G. The Importance of DNA Methylation of Exons on Alternative Splicing. RNA. 2018;: pubmed publisher
..This is the first direct demonstration that DNA methylation of exon-encoding regions is directly involved in regulation of alternative splicing. ..
Naftelberg S, Schor I, Ast G, Kornblihtt A. Regulation of alternative splicing through coupling with transcription and chromatin structure. Annu Rev Biochem. 2015;84:165-98 pubmed publisher
..We focus on evidence for how the kinetics of RNA polymerase II (RNAPII) elongation and the recruitment of splicing factors and adaptor proteins to chromatin components act in coordination to regulate alternative splicing. ..
Yearim A, Gelfman S, Shayevitch R, Melcer S, Glaich O, Mallm J, et al
. HP1 is involved in regulating the global impact of DNA methylation on alternative splicing. Cell Rep. 2015;10:1122-34 pubmed publisher
..Our results demonstrate DNA methylation's significant global influence on mRNA splicing and identify a specific mechanism of splicing regulation mediated by HP1. ..
Lev Maor G, Yearim A, Ast G. The alternative role of DNA methylation in splicing regulation. Trends Genet. 2015;31:274-80 pubmed publisher
..These two mechanisms, however, regulate only a fraction of such events, implying that more underlying mechanisms remain to be found. ..
Donyo M, Hollander D, Abramovitch Z, Naftelberg S, Ast G. Phosphatidylserine enhances IKBKAP transcription by activating the MAPK/ERK signaling pathway. Hum Mol Genet. 2016;25:1307-17 pubmed publisher
..Therefore, compounds that activate the MAPK/ERK signaling pathway could constitute potential treatments for FD. ..
Keren H, Donyo M, Zeevi D, Maayan C, Pupko T, Ast G. Phosphatidylserine increases IKBKAP levels in familial dysautonomia cells. PLoS ONE. 2010;5:e15884 pubmed publisher
..Our results suggest that PS has potential for use as a therapeutic agent for FD. Understanding its mechanism of action may reveal the mechanism underlying the FD disease. ..
Kfir N, Lev Maor G, Glaich O, Alajem A, Datta A, Sze S, et al
. SF3B1 association with chromatin determines splicing outcomes. Cell Rep. 2015;11:618-29 pubmed publisher
..Our findings suggest that the association of SF3B1 with nucleosomes is functionally important for splice-site recognition and that SF3B1 conveys splicing-relevant information embedded in chromatin structure. ..
Hollander D, Naftelberg S, Lev Maor G, Kornblihtt A, Ast G. How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?. Trends Genet. 2016;32:596-606 pubmed publisher
..This may explain how RNAPII and chromatin are involved in spliceosome assembly and how introns lengthened during evolution with a relatively minimal compromise in splicing. ..