Shulamit Levenberg

Summary

Affiliation: Technion-Israel Institute of Technology
Country: Israel

Publications

  1. ncbi request reprint Engineering vascularized skeletal muscle tissue
    Shulamit Levenberg
    Department of Biomedical Engineering, Technion 32000, Haifa, Israel
    Nat Biotechnol 23:879-84. 2005
  2. ncbi request reprint Tissue engineering of vascularized cardiac muscle from human embryonic stem cells
    Oren Caspi
    Sohnis Family Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, Rappaport Family Institute for Research in the Medical Sciences, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, Israel
    Circ Res 100:263-72. 2007
  3. ncbi request reprint Frontier microfluidic techniques for short and long-term single cell analysis
    Jonathan Avesar
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel 32000
    Lab Chip 14:2161-7. 2014
  4. pmc Engineered vascular beds provide key signals to pancreatic hormone-producing cells
    Keren Kaufman-Francis
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel
    PLoS ONE 7:e40741. 2012
  5. ncbi request reprint Engineering blood vessels from stem cells: recent advances and applications
    Shulamit Levenberg
    Faculty of Biomedical Engineering, Technion, Haifa 32000, Israel
    Curr Opin Biotechnol 16:516-23. 2005
  6. doi request reprint Transplantation of a tissue-engineered human vascularized cardiac muscle
    Ayelet Lesman
    Department of Biomedical Engineering, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part A 16:115-25. 2010
  7. doi request reprint Design of well and groove microchannel bioreactors for cell culture
    Natanel Korin
    Biomedical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    Biotechnol Bioeng 102:1222-30. 2009
  8. pmc An engineered muscle flap for reconstruction of large soft tissue defects
    Yulia Shandalov
    Biomedical Engineering and Medicine Departments and Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Proc Natl Acad Sci U S A 111:6010-5. 2014
  9. doi request reprint Engineering vessel-like networks within multicellular fibrin-based constructs
    Ayelet Lesman
    Department of Bio Medical Engineering, Technion Institute of Technology, Haifa 32000, Israel
    Biomaterials 32:7856-69. 2011
  10. doi request reprint Periodic "flow-stop" perfusion microchannel bioreactors for mammalian and human embryonic stem cell long-term culture
    Natanel Korin
    Biomedical Engineering, Technion Israel Institute of Technology, Haifa, 32000, Israel
    Biomed Microdevices 11:87-94. 2009

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Engineering vascularized skeletal muscle tissue
    Shulamit Levenberg
    Department of Biomedical Engineering, Technion 32000, Haifa, Israel
    Nat Biotechnol 23:879-84. 2005
    ..Prevascularization improved the vascularization, blood perfusion and survival of the muscle tissue constructs after transplantation...
  2. ncbi request reprint Tissue engineering of vascularized cardiac muscle from human embryonic stem cells
    Oren Caspi
    Sohnis Family Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, Rappaport Family Institute for Research in the Medical Sciences, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, Israel
    Circ Res 100:263-72. 2007
    ..In summary, this is the first report of the construction of 3D vascularized human cardiac tissue that may have unique applications for studies of cardiac development, function, and tissue replacement therapy...
  3. ncbi request reprint Frontier microfluidic techniques for short and long-term single cell analysis
    Jonathan Avesar
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel 32000
    Lab Chip 14:2161-7. 2014
    ..Developments in incubation have allowed for long-term cell tracking assays to be performed with single cell resolution. The ability of systems to provide chemical isolation or prolonged growth of adherent cells is also discussed. ..
  4. pmc Engineered vascular beds provide key signals to pancreatic hormone-producing cells
    Keren Kaufman-Francis
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel
    PLoS ONE 7:e40741. 2012
    ..These findings present potential strategies for preparation of transplantable islets with increased survival prospects...
  5. ncbi request reprint Engineering blood vessels from stem cells: recent advances and applications
    Shulamit Levenberg
    Faculty of Biomedical Engineering, Technion, Haifa 32000, Israel
    Curr Opin Biotechnol 16:516-23. 2005
    ..Recently, these cells were successfully used to engineer single vessels and to stimulate capillary networks, both in vitro and in vivo...
  6. doi request reprint Transplantation of a tissue-engineered human vascularized cardiac muscle
    Ayelet Lesman
    Department of Biomedical Engineering, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part A 16:115-25. 2010
    ..By utilizing a multicellular preparation we were able to increase biograft vascularization and to show that the preexisting human vessels can become functional and contribute to tissue perfusion...
  7. doi request reprint Design of well and groove microchannel bioreactors for cell culture
    Natanel Korin
    Biomedical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    Biotechnol Bioeng 102:1222-30. 2009
    ..Altogether, the study demonstrates that the proper design and use of microgroove/well bioreactors may be highly beneficial for cell culture assays...
  8. pmc An engineered muscle flap for reconstruction of large soft tissue defects
    Yulia Shandalov
    Biomedical Engineering and Medicine Departments and Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Proc Natl Acad Sci U S A 111:6010-5. 2014
    ....
  9. doi request reprint Engineering vessel-like networks within multicellular fibrin-based constructs
    Ayelet Lesman
    Department of Bio Medical Engineering, Technion Institute of Technology, Haifa 32000, Israel
    Biomaterials 32:7856-69. 2011
    ..Our findings demonstrate that complex biomaterial platform involving fibrin and PLLA/PLGA synthetic scaffold provide a way to enhancing vascularization in-vitro and in-vivo...
  10. doi request reprint Periodic "flow-stop" perfusion microchannel bioreactors for mammalian and human embryonic stem cell long-term culture
    Natanel Korin
    Biomedical Engineering, Technion Israel Institute of Technology, Haifa, 32000, Israel
    Biomed Microdevices 11:87-94. 2009
    ..g., feeder cells secreted factors) to accumulate locally. Thus the developed method may be suitable for long-term culture of sensitive multi-cellular complexes in microsystems...
  11. doi request reprint A microfluidic traps system supporting prolonged culture of human embryonic stem cells aggregates
    Maria Khoury
    Biomedical Engineering, Technion, Haifa, Israel 32000
    Biomed Microdevices 12:1001-8. 2010
    ..The novel microbioreactor presented here can enhance future embryogenesis research by offering tight control of culturing conditions...
  12. doi request reprint Vascularization shaping the heart
    Ayelet Lesman
    Department of Bio Medical Engineering, The Bruce Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa, Israel
    Ann N Y Acad Sci 1188:46-51. 2010
    ..Such muscle-constructs could contribute significantly to the emerging discipline of cardiovascular regenerative medicine as well as to the study of the important role of tissue vascularization...
  13. doi request reprint Vascularization--the conduit to viable engineered tissues
    Tamar Kaully
    Faculty of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part B Rev 15:159-69. 2009
    ..Current paper reviews the progress in each approach and recent achievements toward vascularization of engineered tissues...
  14. doi request reprint Quantifying continuous-flow dielectrophoretic trapping of cells and micro-particles on micro-electrode array
    Lichen Rozitsky
    Russel Berrie Nanotechnology Institute RBNI, Technion Israel Institute of Technology, Haifa, Israel
    Biomed Microdevices 15:859-65. 2013
    ..Also, the generated trapping percentage curve response over a continuous range of frequencies facilitates sorting and detection based on differences other than just the cross-over frequencies. ..
  15. doi request reprint Infantile aphakic glaucoma: a proposed etiologic role of IL-4 and VEGF
    Inbal Michael
    Faculty of Bio Medical Engineering, Technion Israel Institute of Technology, Haifa, Israel
    J Pediatr Ophthalmol Strabismus 48:98-107. 2011
    ..To identify the factors secreted by lens epithelial cells (LECs) responsible for the altered trabecular meshwork (TM) cells and to compare their effect on monocultured TM cells with that of TM cells co-cultured with LECs...
  16. doi request reprint Regulation of stem cell differentiation by control of retinoic acid gradients in hydrospun 3D scaffold
    Roey Tzezana
    Russell Berrie Nanotechnology Institute, Technion, Haifa 32000, Israel
    Macromol Biosci 12:598-607. 2012
    ..This tool bears powerful potential in establishing in vitro simulation models for better understanding the inner workings of the embryo...
  17. ncbi request reprint A layered ultra-porous scaffold for tissue engineering, created via a hydrospinning method
    Roey Tzezana
    Program of Nanotechnology, Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part C Methods 14:281-8. 2008
    ....
  18. ncbi request reprint Biodegradable scaffold fabricated of electrospun albumin fibers: mechanical and biological characterization
    Nora Nseir
    Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part C Methods 19:257-64. 2013
    ..Thus, albumin scaffolds featuring biologically relevant characteristics pose a readily applicable alternative to synthetic scaffolding materials...
  19. pmc Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
    Shulamit Levenberg
    Faculty of Biomedical Engineering, Technion Institute of Technology, Haifa, Israel
    Nat Protoc 5:1115-26. 2010
    ....
  20. doi request reprint Modeling of flow-induced shear stress applied on 3D cellular scaffolds: Implications for vascular tissue engineering
    Ayelet Lesman
    Department of Bio Medical Engineering, Technion Institute of Technology, Haifa, Israel
    Biotechnol Bioeng 105:645-54. 2010
    ..Our results provide in-depth analysis of the microdynamic environment of cells cultured within 3D environments, and thus provide advanced control over tissue development in vitro...
  21. pmc Endothelial potential of human embryonic stem cells
    Shulamit Levenberg
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    Blood 110:806-14. 2007
    ....
  22. doi request reprint Effect of scaffold stiffness on myoblast differentiation
    Meital Levy-Mishali
    Faculty of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part A 15:935-44. 2009
    ..Hence, optimal scaffold stiffness can be tailored by PLLA/PLGA blending to direct specific stages of myoblast differentiation and organization...
  23. ncbi request reprint Engineering human embryonic stem cell differentiation
    Natanel Korin
    Department of Bio Medical Engineering, Technion, Haifa, Israel, 32000
    Biotechnol Genet Eng Rev 24:243-61. 2007
  24. pmc Improved vascular organization enhances functional integration of engineered skeletal muscle grafts
    Jacob Koffler
    Biomedical Engineering Faculty, Technion Israel Institute of Technology, Haifa 32000, Israel
    Proc Natl Acad Sci U S A 108:14789-94. 2011
    ..The enhanced angiogenic response supports enhanced muscle regeneration, maturation, and integration...
  25. doi request reprint Advanced microfluidic droplet manipulation based on piezoelectric actuation
    Jonathan Shemesh
    Biomedical Engineering, Technion, Haifa, Israel
    Biomed Microdevices 12:907-14. 2010
    ..These advantages simplify the adoption of droplet-based microfluidic technology by the scientific community and provide an ideal platform for single cell assays...
  26. doi request reprint Olfactory bulb-derived cells seeded on 3D scaffolds exhibit neurotrophic factor expression and pro-angiogenic properties
    Jacob Blumenthal
    Department of Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel
    Tissue Eng Part A 19:2284-91. 2013
    ..This strategy will enable more efficient therapeutic usage of OB-derived cells for treatment of SCI. ..
  27. doi request reprint Mechanical control of stem cell differentiation
    Dekel Dado
    Biomedical Engineering, Technion, Haifa, 32000, Israel
    Regen Med 7:101-16. 2012
    ....
  28. doi request reprint Coalescence-assisted generation of single nanoliter droplets with predefined composition
    Jonathan Shemesh
    Russell Berrie Nanotechnology Institute, Technion, Haifa, Israel 32000
    Lab Chip 11:3225-30. 2011
    ..The presented platform offers, for the first time, a means to perform droplet-based high-throughput screening in the nanoliter range...
  29. doi request reprint Experimental and theoretical study of selective protein deposition using focused micro laminar flows
    Avishay Bransky
    Biomedical Engineering, Technion Israel Institute of Technology, Haifa, Israel, 32000
    Biomed Microdevices 10:421-8. 2008
    ..This phenomenon is demonstrated here by achieving particularly precise patterning of cells but may be utilized for numerous other applications as well...
  30. ncbi request reprint A parametric study of human fibroblasts culture in a microchannel bioreactor
    Natanel Korin
    Biomedical Engineering, Technion, Haifa, Israel 32000
    Lab Chip 7:611-7. 2007
    ..The presented results demonstrate the importance of understanding the relationship between design parameters and cell behavior in microscale culture system, which vary from physiological and traditional culture conditions...
  31. doi request reprint A microfluidic droplet generator based on a piezoelectric actuator
    Avishay Bransky
    Biomedical Engineering, Technion, Haifa, Israel 32000
    Lab Chip 9:516-20. 2009
    ..As this ratio increases, droplets tend to be generated in bursts instead of individually. The physical mechanisms involved are discussed, providing insight into optimized design of such systems...
  32. doi request reprint Cell-scaffold mechanical interplay within engineered tissue
    Dekel Dado
    The Faculty of Bio Medical Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    Semin Cell Dev Biol 20:656-64. 2009
    ..Further understanding of the reciprocal effects between cells and scaffold will provide both enhanced knowledge regarding the expected properties of engineered tissue and more competent tissue regeneration techniques...
  33. ncbi request reprint Engineering three-dimensional tissue structures using stem cells
    Janet Zoldan
    Biomedical Engineering Department, Technion, Haifa, Israel
    Methods Enzymol 420:381-91. 2006
    ..This chapter describes first steps toward realizing this goal gathered from our experience in growing and differentiating hESCs in three dimensions...
  34. doi request reprint Interactions between trabecular meshwork cells and lens epithelial cells: a possible mechanism in infantile aphakic glaucoma
    Inbal Michael
    Faculty of Bio Medical Engineering, Lorry I Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion Israel Institute of Technology, Haifa, Israel
    Invest Ophthalmol Vis Sci 49:3981-7. 2008
    ..This study focused on the potential role of retained lens material (specifically, exposed lens epithelial cells [LECs]) in undesired changes of the trabecular meshwork (TM) structure and function...
  35. ncbi request reprint Advances in tissue engineering
    Shulamit Levenberg
    Massachusetts Institute of Technology, Chemical Engineering Department, Cambridge, Massachusetts 02139, USA
    Curr Top Dev Biol 61:113-34. 2004
  36. ncbi request reprint Neurotrophin-induced differentiation of human embryonic stem cells on three-dimensional polymeric scaffolds
    Shulamit Levenberg
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Tissue Eng 11:506-12. 2005
    ..In addition, vascular structures were found throughout the engineered tissues when cultured with the neurotrophins, but not in the presence of RA...
  37. ncbi request reprint Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures
    Ali Khademhosseini
    Division of Biological Engineering, Massachusetts Institute of Technology, 45 Carleton Street, E25 342, Cambridge, MA 02139, USA
    Biomaterials 25:3583-92. 2004
    ..Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell-cell interaction studies and tissue engineering applications...
  38. pmc Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds
    Shulamit Levenberg
    Department of Chemical Engineering, Divisions of Health Sciences and Technology, and Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 100:12741-6. 2003
    ..This approach provides a unique culture system for addressing questions in cell and developmental biology, and provides a potential mechanism for creating viable human tissue structures for therapeutic applications...
  39. pmc Endothelial cells derived from human embryonic stem cells
    Shulamit Levenberg
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:4391-6. 2002
    ....
  40. ncbi request reprint Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells
    Daniel G Anderson
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Biotechnol 22:863-6. 2004
    ..We simultaneously characterize over 1,700 human embryonic stem cell-material interactions and identify a host of unexpected materials effects that offer new levels of control over human embryonic stem cell behavior...