Avigdor Shafferman

Summary

Affiliation: Israel Institute for Biological Research
Country: Israel

Publications

  1. doi request reprint Aging-resistant organophosphate bioscavenger based on polyethylene glycol-conjugated F338A human acetylcholinesterase
    Ohad Mazor
    Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Mol Pharmacol 74:755-63. 2008
  2. pmc Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens
    N Ariel
    Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Infect Immun 71:4563-79. 2003
  3. pmc Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen
    Orit Gat
    Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Infect Immun 74:3987-4001. 2006
  4. ncbi request reprint Functional requirements for the optimal catalytic configuration of the AChE active center
    Avigdor Shafferman
    Department Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Chem Biol Interact 157:123-31. 2005
  5. doi request reprint Characterization of Bacillus anthracis iron-regulated surface determinant (Isd) proteins containing NEAT domains
    Orit Gat
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Mol Microbiol 70:983-99. 2008
  6. doi request reprint HtrA is a major virulence determinant of Bacillus anthracis
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Mol Microbiol 81:1542-59. 2011
  7. pmc Yersinia pestis endowed with increased cytotoxicity is avirulent in a bubonic plague model and induces rapid protection against pneumonic plague
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 4:e5938. 2009
  8. pmc The NlpD lipoprotein is a novel Yersinia pestis virulence factor essential for the development of plague
    Avital Tidhar
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 4:e7023. 2009
  9. doi request reprint Neutralization of Yersinia pestis-mediated macrophage cytotoxicity by anti-LcrV antibodies and its correlation with protective immunity in a mouse model of bubonic plague
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Vaccine 26:1616-25. 2008
  10. doi request reprint T cells play an essential role in anti-F1 mediated rapid protection against bubonic plague
    Yinon Levy
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Vaccine 29:6866-73. 2011

Collaborators

Detail Information

Publications57

  1. doi request reprint Aging-resistant organophosphate bioscavenger based on polyethylene glycol-conjugated F338A human acetylcholinesterase
    Ohad Mazor
    Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Mol Pharmacol 74:755-63. 2008
    ....
  2. pmc Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens
    N Ariel
    Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Infect Immun 71:4563-79. 2003
    ..This study demonstrates that a combination of the bioinformatic and proteomic strategies may be useful in promoting the development of next generation anthrax vaccine...
  3. pmc Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen
    Orit Gat
    Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Infect Immun 74:3987-4001. 2006
    ..We list more than 30 novel B. anthracis immunoreactive virulence-related proteins which could be useful in diagnosis, pathogenesis studies, and future anthrax vaccine development...
  4. ncbi request reprint Functional requirements for the optimal catalytic configuration of the AChE active center
    Avigdor Shafferman
    Department Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Chem Biol Interact 157:123-31. 2005
    ....
  5. doi request reprint Characterization of Bacillus anthracis iron-regulated surface determinant (Isd) proteins containing NEAT domains
    Orit Gat
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Mol Microbiol 70:983-99. 2008
    ..Studies with recombinant IsdK demonstrate specific binding to haemoglobin...
  6. doi request reprint HtrA is a major virulence determinant of Bacillus anthracis
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Mol Microbiol 81:1542-59. 2011
    ..On a practical note, it is demonstrated that the attenuated Vollum ΔhtrA is highly efficacious in protecting guinea pigs against a lethal anthrax challenge...
  7. pmc Yersinia pestis endowed with increased cytotoxicity is avirulent in a bubonic plague model and induces rapid protection against pneumonic plague
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 4:e5938. 2009
    ..These observations have novel implications for the development of vaccines/therapies against Y. pestis and shed new light on the virulence strategies of Y. pestis in nature...
  8. pmc The NlpD lipoprotein is a novel Yersinia pestis virulence factor essential for the development of plague
    Avital Tidhar
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 4:e7023. 2009
    ..Thus it could serve as a basis for a very potent live vaccine against bubonic and pneumonic plague...
  9. doi request reprint Neutralization of Yersinia pestis-mediated macrophage cytotoxicity by anti-LcrV antibodies and its correlation with protective immunity in a mouse model of bubonic plague
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Vaccine 26:1616-25. 2008
    ..The impact of overall findings on the potential use of serum neutralizing activity as a correlate of protective immunity is discussed...
  10. doi request reprint T cells play an essential role in anti-F1 mediated rapid protection against bubonic plague
    Yinon Levy
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Vaccine 29:6866-73. 2011
    ..These data extend our understanding of the immune mechanisms required for the development of a rapid and effective post-exposure therapy against plague...
  11. ncbi request reprint Polyethylene-glycol conjugated recombinant human acetylcholinesterase serves as an efficacious bioscavenger against soman intoxication
    Chanoch Kronman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Toxicology 233:40-6. 2007
    ....
  12. pmc Generation of Yersinia pestis attenuated strains by signature-tagged mutagenesis in search of novel vaccine candidates
    Yehuda Flashner
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, 74100, Israel
    Infect Immun 72:908-15. 2004
    ..This Kimberley53pcm mutant was superior to the EV76 live vaccine strain because it induced 10- to 100-fold-higher antibody titers to the protective V and F1 antigens and because it conferred efficacious protective immunity...
  13. pmc Interaction of Yersinia pestis with macrophages: limitations in YopJ-dependent apoptosis
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona, 74100, Israel
    Infect Immun 74:3239-50. 2006
    ..enterocolitica WA O:8. We suggest that the relative inferiority of Y. pestis in triggering cell death in macrophages may be advantageous for its in vivo propagation in the early stages of infection...
  14. ncbi request reprint Vaccination with plasmid DNA expressing the Yersinia pestis capsular protein F1 protects mice against plague
    Haim Grosfeld
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona, Israel 74100
    Adv Exp Med Biol 529:423-4. 2003
  15. pmc A novel mechanism for antibody-based anthrax toxin neutralization: inhibition of prepore-to-pore conversion
    Adva Mechaly
    Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    J Biol Chem 287:32665-73. 2012
    ..In conclusion, these experiments demonstrate that cAb29 exerts its potent neutralizing activity in a unique manner by blocking the prepore-to-pore conversion process...
  16. ncbi request reprint Is aromaticity essential for trapping the catalytic histidine 447 in human acetylcholinesterase?
    Dana Kaplan
    Department of Biochemistry and Molecular Biology, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Biochemistry 43:3129-36. 2004
    ..The results also underscore the role of the acyl pocket subsite of cholinesterases in maintaining the catalytically viable conformation of the catalytic histidine...
  17. ncbi request reprint Identification of chromosomally encoded membranal polypeptides of Bacillus anthracis by a proteomic analysis: prevalence of proteins containing S-layer homology domains
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Proteomics 4:677-91. 2004
    ....
  18. doi request reprint Next generation OP-bioscavengers: a circulatory long-lived 4-PEG hypolysine mutant of F338A-HuAChE with optimal pharmacokinetics and pseudo-catalytic characteristics
    Chanoch Kronman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, 24 Rueven St, P O Box 19, Ness Ziona 74100, Israel
    Chem Biol Interact 187:253-8. 2010
    ..This selected 4-PEG F338A-AChE can serve as a paradigm for new generation OP-bioscavengers, specifically tailored for prophylactic treatment against toxic OP-agents...
  19. ncbi request reprint The solute-binding component of a putative Mn(II) ABC transporter (MntA) is a novel Bacillus anthracis virulence determinant
    Orit Gat
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, PO Box 19, Ness Ziona, 74100, Israel
    Mol Microbiol 58:533-51. 2005
    ..We propose that MntA is a novel B. anthracis virulence determinant essential for the development of anthrax disease, and that B. anthracisDeltamntA strains have the potential to serve as platform for future live attenuated vaccines...
  20. ncbi request reprint Evaluation of protective immunity induced by Yersinia enterocolitica type-III secretion system mutants
    Emanuelle Mamroud
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona, Israel 74100
    Adv Exp Med Biol 529:425-30. 2003
  21. pmc Differential contribution of Bacillus anthracis toxins to pathogenicity in two animal models
    Haim Levy
    Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel
    Infect Immun 80:2623-31. 2012
    ..anthracis virulence in general and in particular for the design of relevant next-generation anthrax vaccines...
  22. pmc Accommodation of physostigmine and its analogues by acetylcholinesterase is dominated by hydrophobic interactions
    Dov Barak
    Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Biochem J 417:213-22. 2009
    ..The present study demonstrates that certain charged and uncharged ligands, like analogues of physostigmine and physovenine, seem to be accommodated by the enzyme mostly through hydrophobic interactions...
  23. ncbi request reprint Controlled concealment of exposed clearance and immunogenic domains by site-specific polyethylene glycol attachment to acetylcholinesterase hypolysine mutants
    Ofer Cohen
    Department of Biochemistry, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    J Biol Chem 282:35491-501. 2007
    ....
  24. doi request reprint Progress and novel strategies in vaccine development and treatment of anthrax
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Immunol Rev 239:221-36. 2011
    ....
  25. ncbi request reprint In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis
    Orit Gat
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Methods Mol Biol 375:211-33. 2007
    ..Protocols will be provided for application of these methods to analyze the anti-B. anthracis antibody repertoire of hyperimmune sera or sera from convalescent and from DNA-vaccinated animals...
  26. pmc Flexibility versus "rigidity" of the functional architecture of AChE active center
    Avigdor Shafferman
    Department Biochemistry and Molecular Biology, Israel Institute for Biological Research, Ness Ziona, Israel
    Chem Biol Interact 175:166-72. 2008
    ..These studies suggest that the combination of "rigidity" and flexibility within the HuAChE gorge are an essential element of its molecular design...
  27. ncbi request reprint Lessons from functional analysis of AChE covalent and noncovalent inhibitors for design of AD therapeutic agents
    Dov Barak
    Department of Organic Chemistry, Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Chem Biol Interact 157:219-26. 2005
    ..Thus, elucidation of the functional architecture of the HuAChE active center is bound to be of considerable utility in the current effort to design novel covalent AChE inhibitors as therapeutics for Alzheimer's disease (AD)...
  28. pmc Discordance in the effects of Yersinia pestis on the dendritic cell functions manifested by induction of maturation and paralysis of migration
    Baruch Velan
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Infect Immun 74:6365-76. 2006
    ..pestis harboring the plasmid failed to do so. Taken together, these results suggest that virulence plasmid-dependent impairment of DC migration is the major mechanism utilized by Y. pestis to subvert DC function...
  29. ncbi request reprint Comparison of polyethylene glycol-conjugated recombinant human acetylcholinesterase and serum human butyrylcholinesterase as bioscavengers of organophosphate compounds
    Ofer Cohen
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Mol Pharmacol 70:1121-31. 2006
    ..3-1.5 LD50). These findings together with the observations that the PEGylated rHuAChE exhibits unaltered biodistribution and high bioavailability present a case for using PEGylated rHuAChE as a very efficacious bioscavenger of OP agents...
  30. pmc Amino acid domains control the circulatory residence time of primate acetylcholinesterases in rhesus macaques (Macaca mulatta)
    Ofer Cohen
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Biochem J 378:117-28. 2004
    ..This extended residence time is similar to that displayed by native macaque butyrylcholinesterase (9950 min), which is the prevalent cholinesterase form in the circulation of adult macaques...
  31. ncbi request reprint Effects of spontaneous deamidation on the cytotoxic activity of the Bacillus anthracis protective antigen
    Gil Zomber
    Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    J Biol Chem 280:39897-906. 2005
    ..We suggest that PA inactivation during storage is associated with susceptible deamidation sites, which are intimately involved in both mechanisms of PA cleavage by furin and PA-receptor binding...
  32. ncbi request reprint Identification of genes involved in Yersinia pestis virulence by signature-tagged mutagenesis
    Yehuda Flashner
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, P O Box 19, Ness Ziona, Israel 74100
    Adv Exp Med Biol 529:31-3. 2003
  33. pmc Yopp-Expressing Variant ofY. pestis Activates a Potent Innate Immune Response Affording Cross-Protection against Yersiniosis and Tularemia
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 8:e83560. 2013
    ....
  34. pmc A single cidofovir treatment rescues animals at progressive stages of lethal orthopoxvirus disease
    Tomer Israely
    Israel Institute for Biological Research, Ness Ziona, Israel
    Virol J 9:119. 2012
    ..e. either alone or in conjunction with active vaccination, can rescue ECTV infected mice...
  35. pmc Clusters versus affinity-based approaches in F. tularensis whole genome search of CTL epitopes
    Anat Zvi
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 7:e36440. 2012
    ..tularensis dataset of known CTL epitopes. The generality and power of the high density cluster-based approach suggest that it can be a valuable tool for identification of novel CTLs in proteomes of other bacterial pathogens...
  36. ncbi request reprint The aromatic "trapping" of the catalytic histidine is essential for efficient catalysis in acetylcholinesterase
    Dov Barak
    Department of Organic Chemistry, Israel Institute for Biological Research, Ness Ziona, 74100, Israel
    Biochemistry 41:8245-52. 2002
    ....
  37. ncbi request reprint Host-regulated disposition of mammalian AChEs
    Chanoch Kronman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Chem Biol Interact 157:51-5. 2005
    ....
  38. doi request reprint Proteomic studies of Bacillus anthracis
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Future Microbiol 4:983-98. 2009
    ..anthracis protein targets for further evaluation in detection, diagnostics, therapy and prophylaxis, and contributed to the elucidation of some aspects of the pathogenesis of the disease...
  39. pmc Identification of in vivo-expressed immunogenic proteins by serological proteome analysis of the Bacillus anthracis secretome
    Theodor Chitlaru
    Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Infect Immun 75:2841-52. 2007
    ..anthracis strain Vollum spores...
  40. pmc Consequences of delayed ciprofloxacin and doxycycline treatment regimens against Francisella tularensis airway infection
    Shahar Rotem
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    Antimicrob Agents Chemother 56:5406-8. 2012
    ..A delay in onset of both antibiotic treatments allowed the rescue of all LVS-infected animals. However, for animals infected with SchuS4, only ciprofloxacin was efficacious and prolongation of treatment rescued all animals...
  41. pmc Interrelationship between dendritic cell trafficking and Francisella tularensis dissemination following airway infection
    Erez Bar-Haim
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS Pathog 4:e1000211. 2008
    ..tularensis in the early stages of infection, and that DC trafficking plays a role in pathogen dissemination. This underscores the therapeutic potential of DC migration impairing drugs in tularemia treatment...
  42. pmc Contribution of immunological memory to protective immunity conferred by a Bacillus anthracis protective antigen-based vaccine
    Hadar Marcus
    Department of Biotechnology, Israel Institute for Biological Research, 74100 Ness Ziona, Israel
    Infect Immun 72:3471-7. 2004
    ..Therefore, to allow for the establishment of memory-dependent protection prior to the expected onset of disease, booster immunizations should not be used without concomitant antimicrobial treatment in postexposure scenarios...
  43. ncbi request reprint The role of AChE active site gorge in determining stereoselectivity of charged and noncharged VX enantiomers
    Arie Ordentlich
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Chem Biol Interact 157:191-8. 2005
    ..Such different orientations of the charged leaving group in the HuAChE adducts of the VX enantiomers seem to be a consequence of intramolecular interactions with the bulky phosphorus alkoxy group...
  44. pmc Effective protective immunity to Yersinia pestis infection conferred by DNA vaccine coding for derivatives of the F1 capsular antigen
    Haim Grosfeld
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 74100, Israel
    Infect Immun 71:374-83. 2003
    ..Taken together, these observations indicate that a tailored genetic vaccine based on a bacterial protein can be used to confer protection against plague in mice without resorting to regimens involving the use of purified proteins...
  45. pmc Identification and characterization of novel and potent transcription promoters of Francisella tularensis
    Galia Zaide
    Israel Institute for Biological Research, P O Box 19, Ness Ziona 74100, Israel
    Appl Environ Microbiol 77:1608-18. 2011
    ..tularensis intended to gain better understanding of the mechanisms involved in pathogenesis and virulence, as well as for vaccine development studies...
  46. doi request reprint Effective post-exposure protection against lethal orthopoxviruses infection by vaccinia immune globulin involves induction of adaptive immune response
    Shlomo Lustig
    Department of Infectious Diseases, Israel Institute for Biological Research, Israel
    Vaccine 27:1691-9. 2009
    ..Vaccine efficiency is not impaired, providing a non-prohibitive VIG dose is used. Thus, VIG can be used as a prophylactic measure against post-vaccinal complications but could also serve for post-exposure treatment against smallpox...
  47. pmc Whole-genome immunoinformatic analysis of F. tularensis: predicted CTL epitopes clustered in hotspots are prone to elicit a T-cell response
    Anat Zvi
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 6:e20050. 2011
    ..tularensis, could be applied in immunoinformatic analyses of many other complex pathogen genomes...
  48. pmc Protective Immunity against Lethal F. tularensis holarctica LVS Provided by Vaccination with Selected Novel CD8(+) T Cell Epitopes
    Shahar Rotem
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel
    PLoS ONE 9:e85215. 2014
    ..Our approach provides a proof-of-principle for selecting and generating a multi-epitpoe CD8 T cell-stimulating vaccine against a model intracellular bacterium. ..
  49. doi request reprint Postexposure immunization with modified vaccinia virus Ankara or conventional Lister vaccine provides solid protection in a murine model of human smallpox
    Nir Paran
    Israel Institute for Biological Research, Ness Ziona, Israel
    J Infect Dis 199:39-48. 2009
    ..Scenarios of sudden biothreats have prompted demand for rapidly protective vaccination. However, the feasibility of short-term vaccination (i.e., vaccination shortly before exposure) with vaccinia virus (VACV) is uncertain...
  50. pmc Overloading and removal of N-glycosylation targets on human acetylcholinesterase: effects on glycan composition and circulatory residence time
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, 74100, Israel
    Biochem J 363:619-31. 2002
    ..This study provides evidence that glycan loading, together with N-glycan terminal processing and enzyme subunit oligomerization, operate in a hierarchical and concerted manner in determining the pharmacokinetic characteristics of AChE...
  51. ncbi request reprint The NheA component of the non-hemolytic enterotoxin of Bacillus cereus is produced by Bacillus anthracis but is not required for virulence
    Itai Mendelson
    United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA
    Microb Pathog 37:149-54. 2004
    ..anthracis produces and secretes the NheA subunit of NHE. A NheA deletion mutant was not attenuated in the guinea pig suggesting that NheA is not absolutely required for virulence...
  52. pmc Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activities
    Theodor Chitlaru
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona
    J Bacteriol 188:3551-71. 2006
    ....
  53. ncbi request reprint Disparity between Yersinia pestis and Yersinia enterocolitica O:8 in YopJ/YopP-dependent functions
    Ayelet Zauberman
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona
    Adv Exp Med Biol 603:312-20. 2007
    ..pestis and Y. enterocolitica O:8 virulent strains shows some critical disparity in YopJ/YopP-related effects on immune cells. Involvement of yopJ in virulence was evaluated in mouse model of bubonic plague...
  54. ncbi request reprint Construction of a rhamnose mutation in Bacillus anthracis affects adherence to macrophages but not virulence in guinea pigs
    Joel A Bozue
    Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, USAMRIID, Ft Detrick, MD 21702, USA
    Microb Pathog 38:1-12. 2005
    ..These results suggest that the incorporation of rhamnose into the spore coat of B. anthracis is required for optimal interaction with macrophages but is not required for full virulence in this animal model...
  55. pmc Inhibition of human acetyl- and butyrylcholinesterase by novel carbamates of (-)- and (+)-tetrahydrofurobenzofuran and methanobenzodioxepine
    Weiming Luo
    Drug Design and Development Section, Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    J Med Chem 49:2174-85. 2006
    ....
  56. ncbi request reprint Stereoselectivity toward VX is determined by interactions with residues of the acyl pocket as well as of the peripheral anionic site of AChE
    Arie Ordentlich
    Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, 74100 Israel
    Biochemistry 43:11255-65. 2004
    ..The results from kinetic studies and molecular simulations suggest that the differential reactivity toward the VX enantiomers is mainly a result of a different interaction of the charged leaving group with Asp74...
  57. ncbi request reprint Pressure and heat inactivation of recombinant human acetylcholinesterase. Importance of residue E202 for enzyme stability
    Cécile Cléry-Barraud
    Centre de Recherches du Service de Sante des Armees, Unite d Enzymologie, France
    Eur J Biochem 269:4297-307. 2002
    ..In conclusion, pressure and thermal stability of mutants provided evidence that the residue E202 is a determinant of structural and functional stability of HuAChE...