Varda Shoshan-Barmatz

Summary

Affiliation: Ben-Gurion University of the Negev
Country: Israel

Publications

  1. doi request reprint VDAC, a multi-functional mitochondrial protein regulating cell life and death
    Varda Shoshan-Barmatz
    Department of Life Sciences, and the National Institute for Biotechnology in the Negev NIBN, Ben Gurion University, Beer Sheva, Israel
    Mol Aspects Med 31:227-85. 2010
  2. pmc VDAC1-based peptides: novel pro-apoptotic agents and potential therapeutics for B-cell chronic lymphocytic leukemia
    T Prezma
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Beersheva, Israel
    Cell Death Dis 4:e809. 2013
  3. pmc VDAC1: from structure to cancer therapy
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev Beer Sheva, Israel The National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev Beer Sheva, Israel
    Front Oncol 2:164. 2012
  4. ncbi request reprint Mitochondrial VDAC1: function in cell life and death and a target for cancer therapy
    V Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Curr Med Chem 19:714-35. 2012
  5. ncbi request reprint Retina expresses a novel variant of the ryanodine receptor
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Eur J Neurosci 26:3113-25. 2007
  6. doi request reprint Key regions of VDAC1 functioning in apoptosis induction and regulation by hexokinase
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Biochim Biophys Acta 1787:421-30. 2009
  7. doi request reprint Uncovering the role of VDAC in the regulation of cell life and death
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    J Bioenerg Biomembr 40:183-91. 2008
  8. ncbi request reprint The voltage-dependent anion channel (VDAC): function in intracellular signalling, cell life and cell death
    V Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Curr Pharm Des 12:2249-70. 2006
  9. doi request reprint Hexokinase-I protection against apoptotic cell death is mediated via interaction with the voltage-dependent anion channel-1: mapping the site of binding
    Salah Abu-Hamad
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 283:13482-90. 2008
  10. pmc In self-defence: hexokinase promotes voltage-dependent anion channel closure and prevents mitochondria-mediated apoptotic cell death
    Heftsi Azoulay-Zohar
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biochem J 377:347-55. 2004

Collaborators

Detail Information

Publications47

  1. doi request reprint VDAC, a multi-functional mitochondrial protein regulating cell life and death
    Varda Shoshan-Barmatz
    Department of Life Sciences, and the National Institute for Biotechnology in the Negev NIBN, Ben Gurion University, Beer Sheva, Israel
    Mol Aspects Med 31:227-85. 2010
    ..This review also provides insight into the potential of VDAC1 as a rational target for new therapeutics...
  2. pmc VDAC1-based peptides: novel pro-apoptotic agents and potential therapeutics for B-cell chronic lymphocytic leukemia
    T Prezma
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Beersheva, Israel
    Cell Death Dis 4:e809. 2013
    ..In this manner, three optimized peptides suitable for in vivo studies were identified. This study thus reveals the potential of VDAC1-based peptides as an innovative and effective anti-CLL therapy. ..
  3. pmc VDAC1: from structure to cancer therapy
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev Beer Sheva, Israel The National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev Beer Sheva, Israel
    Front Oncol 2:164. 2012
    ..These and other functions point to VDAC1 as a rational target for the development of a new generation of therapeutics...
  4. ncbi request reprint Mitochondrial VDAC1: function in cell life and death and a target for cancer therapy
    V Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Curr Med Chem 19:714-35. 2012
    ..These and other functions point to VDAC1 as being a rational target for the development of a new generation of therapeutics...
  5. ncbi request reprint Retina expresses a novel variant of the ryanodine receptor
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Eur J Neurosci 26:3113-25. 2007
    ..The restricted localization of the outer segment RyR to the disk rim suggests that its activation mechanism involves a coupling between retinal RyR and the cGMP-gated channel...
  6. doi request reprint Key regions of VDAC1 functioning in apoptosis induction and regulation by hexokinase
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Biochim Biophys Acta 1787:421-30. 2009
    ..Displacing HK, serving as the 'guardian of the mitochondrion', from its binding site on VDAC1 may thus be exploited as an approach to cancer therapy...
  7. doi request reprint Uncovering the role of VDAC in the regulation of cell life and death
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    J Bioenerg Biomembr 40:183-91. 2008
    ....
  8. ncbi request reprint The voltage-dependent anion channel (VDAC): function in intracellular signalling, cell life and cell death
    V Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    Curr Pharm Des 12:2249-70. 2006
    ..This review provides insights into the central role of VDAC in cell life and death and emphasizes its function in the regulation of mitochondria-mediated apoptosis and, thereby, its potential as a rational target for new therapeutics...
  9. doi request reprint Hexokinase-I protection against apoptotic cell death is mediated via interaction with the voltage-dependent anion channel-1: mapping the site of binding
    Salah Abu-Hamad
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 283:13482-90. 2008
    ..Finally, these findings suggest that interference with the binding of HK-I to mitochondria by VDAC1-derived peptides may offer a novel strategy by which to potentiate the efficacy of conventional chemotherapeutic agents...
  10. pmc In self-defence: hexokinase promotes voltage-dependent anion channel closure and prevents mitochondria-mediated apoptotic cell death
    Heftsi Azoulay-Zohar
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biochem J 377:347-55. 2004
    ....
  11. doi request reprint Voltage-dependent anion channel 1-based peptides interact with hexokinase to prevent its anti-apoptotic activity
    Laetitia Arzoine
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel 84105
    J Biol Chem 284:3946-55. 2009
    ..Moreover, VDAC1-based peptides interfering with HK-mediated anti-apoptotic activity may potentiate the efficacy of conventional chemotherapeutic agents...
  12. ncbi request reprint Localization of the voltage-dependent anion channel-1 Ca2+-binding sites
    Adrian Israelson
    Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cell Calcium 41:235-44. 2007
    ..Furthermore, AzRu protection against apoptosis involves interaction with these residues. Photoreactive AzRu represents an important tool for identifying novel Ca(2+)-binding proteins and localizing their Ca(2+)-binding sites...
  13. doi request reprint VDAC1 cysteine residues: topology and function in channel activity and apoptosis
    Lior Aram
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Biochem J 427:445-54. 2010
    ....
  14. ncbi request reprint An N-terminal nucleotide-binding site in VDAC1: involvement in regulating mitochondrial function
    Galit Yehezkel
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    J Cell Physiol 212:551-61. 2007
    ..Finally, involvement of the VDAC1-NBS in the control of mitochondrial ATP synthesis, cell growth and viability is discussed...
  15. doi request reprint The VDAC1 N-terminus is essential both for apoptosis and the protective effect of anti-apoptotic proteins
    Salah Abu-Hamad
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
    J Cell Sci 122:1906-16. 2009
    ..These results show VDAC1 to be a component of the apoptosis machinery and offer new insight into the mechanism of cytochrome c release and how anti-apoptotic proteins regulate apoptosis and promote tumor cell survival...
  16. ncbi request reprint Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1
    Adrian Israelson
    Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cell Calcium 43:196-204. 2008
    ..Our results thus clearly indicate that RuR protects against cell death via a direct interaction with VDAC1 to inhibit cytochrome c release and subsequent cell death...
  17. ncbi request reprint Apoptosis is regulated by the VDAC1 N-terminal region and by VDAC oligomerization: release of cytochrome c, AIF and Smac/Diablo
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biochim Biophys Acta 1797:1281-91. 2010
    ....
  18. ncbi request reprint The voltage-dependent anion channel: characterization, modulation, and role in mitochondrial function in cell life and death
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cell Biochem Biophys 39:279-92. 2003
    ....
  19. pmc Voltage-dependent anion channel 1-based peptides interact with Bcl-2 to prevent antiapoptotic activity
    Nir Arbel
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 285:6053-62. 2010
    ..Moreover, these findings suggest that interfering with the binding of Bcl-2 to mitochondria by VDAC1-based peptides may serve to potentiate the efficacy of conventional chemotherapeutic agents...
  20. ncbi request reprint Novel ryanodine-binding properties in mammalian retina
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Int J Biochem Cell Biol 37:1681-95. 2005
    ..Its presence in retina suggests that this protein might play a role in controlling intracellular Ca2+ concentration...
  21. ncbi request reprint Glutamate interacts with VDAC and modulates opening of the mitochondrial permeability transition pore
    Dan Gincel
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Bioenerg Biomembr 36:179-86. 2004
    ..The interaction of L-glutamate with VDAC and its inhibition of VDAC's channel activity and PTP opening suggest that glutamate may also act as an intracellular messenger in the mitochondria-mediated apoptotic pathway...
  22. doi request reprint New fluorescent reagents specific for Ca(2+)-binding proteins
    Danya Ben-Hail
    Department of Life Sciences and the NIBN, Ben Gurion University, Beer Sheva 84105, Israel
    Biochem Biophys Res Commun 426:158-64. 2012
    ..Such reagents will assist in characterizing Ca(2+)-binding proteins, thereby facilitating better understanding of the function of Ca(2+) as a key bio-regulator...
  23. ncbi request reprint Retinal voltage-dependent anion channel: characterization and cellular localization
    Dan Gincel
    Department of Life Sciences and Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
    Invest Ophthalmol Vis Sci 43:2097-104. 2002
    ..To characterize and localize retinal voltage-dependent anion channel (VDAC) and to understand its possible contribution to mitochondrial function and dysfunction...
  24. ncbi request reprint Azido ruthenium: a new photoreactive probe for calcium-binding proteins
    Adrian Israelson
    Department of Life Sciences, The Zlotowski Center for Neuroscience and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Nat Protoc 1:111-7. 2006
    ..The preparation of AzRu takes approximately 2-3 days...
  25. doi request reprint The role of calcium in VDAC1 oligomerization and mitochondria-mediated apoptosis
    Nurit Keinan
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Biochim Biophys Acta 1833:1745-54. 2013
    ..This article is part of a Special Issue entitled: 12th European Symposium on Calcium...
  26. pmc Mediation of the antiapoptotic activity of Bcl-xL protein upon interaction with VDAC1 protein
    Nir Arbel
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 287:23152-61. 2012
    ..These findings suggest that interfering with Bcl-xL binding to the mitochondria by VDAC1-based peptides may serve to induce apoptosis in cancer cells and to potentiate the efficacy of conventional chemotherapeutic agents...
  27. pmc The expression level of the voltage-dependent anion channel controls life and death of the cell
    Salah Abu-Hamad
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Proc Natl Acad Sci U S A 103:5787-92. 2006
    ....
  28. pmc Oligomeric states of the voltage-dependent anion channel and cytochrome c release from mitochondria
    Ran Zalk
    Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
    Biochem J 386:73-83. 2005
    ..Thus the results indicate that VDAC exists in a dynamic equilibrium between dimers and tetramers and suggest that oligomeric VDAC may be involved in mitochondria-mediated apoptosis...
  29. ncbi request reprint A photoactivable probe for calcium binding proteins
    Adrian Israelson
    Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva 84713, Israel
    Chem Biol 12:1169-78. 2005
    ..AzRu is a photoreactive reagent that provides an approach for identification of Ca2+ binding proteins, characterization of their binding sites, and exploration of new Ca2+-dependent processes...
  30. ncbi request reprint Subcellular localization of VDAC in mitochondria and ER in the cerebellum
    Varda Shoshan-Barmatz
    Department of Life Sciences and Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, P O Box 653, Beer Sheva 84105, Israel
    Biochim Biophys Acta 1657:105-14. 2004
    ....
  31. ncbi request reprint Dominant-negative VDAC1 mutants reveal oligomeric VDAC1 to be the active unit in mitochondria-mediated apoptosis
    Asaf Mader
    Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biochem J 429:147-55. 2010
    ..These results suggest that E72Q-VDAC1 has a dominant-negative effect and implies that VDAC1 homo-oligomerization, involving intermolecular interactions, might be involved in the apoptotic process...
  32. doi request reprint VDAC, a multi-functional mitochondrial protein as a pharmacological target
    Varda Shoshan-Barmatz
    Department of Life Sciences and the NIBN, Ben Gurion University, Beer Sheva, Israel
    Mitochondrion 12:24-34. 2012
    ..Additionally, we provide insight into the potential of VDAC1 as a rational target for novel therapeutics...
  33. pmc Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis
    Nurit Keinan
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Mol Cell Biol 30:5698-709. 2010
    ..Thus, targeting the oligomeric status of VDAC, and hence apoptosis, offers a therapeutic strategy for combating cancers and neurodegenerative diseases...
  34. ncbi request reprint Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles
    Ran Zalk
    Department of Life Sciences, National Institute for Biotechnology, Negev and Zlotowski Center for Neuroscience, Ben Gurion University, Life Sciences Building 40, 84105 Beer Sheva, Israel
    FEBS Lett 580:5894-8. 2006
    ..These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems...
  35. doi request reprint Structure-based analysis of VDAC1: N-terminus location, translocation, channel gating and association with anti-apoptotic proteins
    Shay Geula
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biochem J 444:475-85. 2012
    ..N-terminal mobility may thus contribute to channel gating and interaction with anti-apoptotic proteins...
  36. ncbi request reprint Downregulation of voltage-dependent anion channel-1 expression by RNA interference prevents cancer cell growth in vivo
    Inbar Koren
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
    Cancer Biol Ther 9:1046-52. 2010
    ..Taken together, the results of this study demonstrate, for the first time, the anticancer therapeutic potential of VDAC1 downregulation by means of shRNA...
  37. ncbi request reprint Nucleotide-binding sites in the voltage-dependent anion channel: characterization and localization
    Galit Yehezkel
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 281:5938-46. 2006
    ..Thus, the presence of two or more nucleotide-binding sites in VDAC is suggested, and their possible function in the control of VDAC activity, and, thereby, of outer mitochondrial membrane permeability is discussed...
  38. doi request reprint Oligomerization of the mitochondrial protein VDAC1: from structure to function and cancer therapy
    Varda Shoshan-Barmatz
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Prog Mol Biol Transl Sci 117:303-34. 2013
    ....
  39. pmc The synaptic vesicle protein synaptophysin: purification and characterization of its channel activity
    Dan Gincel
    Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Biophys J 83:3223-9. 2002
    ..The availability of large amounts of purified synaptophysin and of its characteristic channel properties might help to establish the role of synaptophysin in synaptic transmission...
  40. pmc Structure-based analysis of VDAC1 protein: defining oligomer contact sites
    Shay Geula
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    J Biol Chem 287:2179-90. 2012
    ..Dissection of VDAC1 dimerization/oligomerization as presented here provides structural insight into the oligomeric status of cellular VDAC1 under physiological and apoptotic conditions...
  41. pmc NCLX is an essential component of mitochondrial Na+/Ca2+ exchange
    Raz Palty
    Department of Physiology, and Zlotowski Center for Neuroscience, Ben Gurion University of Negev, Beer Sheva 84105, Israel
    Proc Natl Acad Sci U S A 107:436-41. 2010
    ..Taken together, our results converge to the conclusion that NCLX is the long-sought mitochondrial Na(+)/Ca(2+) exchanger...
  42. pmc ATP-binding sites in brain p97/VCP (valosin-containing protein), a multifunctional AAA ATPase
    Ran Zalk
    Department of Life Sciences and Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
    Biochem J 374:473-80. 2003
    ..Conformational changes induced by an alternating site mechanism for ATP binding are suggested as a molecular mechanism for coupling ATP binding to the diverse activities of the AAA family...
  43. doi request reprint Measurement of mitochondrial Ca2+ transport mediated by three transport proteins: VDAC1, the Na+/Ca2+ exchanger, and the Ca2+ uniporter
    Danya Ben-Hail
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Cold Spring Harb Protoc 2014:161-6. 2014
    ..The Ca(2+)-transport assays can be performed under various conditions and in combination with inhibitors, allowing detailed characterization of the transport activity of interest. ..
  44. doi request reprint Reconstitution of purified VDAC1 into a lipid bilayer and recording of channel conductance
    Danya Ben-Hail
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Cold Spring Harb Protoc 2014:100-5. 2014
    ..This system is so efficient that the activity of even a single channel can be detected, allowing for study of VDAC1 at the molecular level. ..
  45. doi request reprint Purification of VDAC1 from rat liver mitochondria
    Danya Ben-Hail
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Cold Spring Harb Protoc 2014:94-9. 2014
    ..Furthermore, the isolated mitochondria can be used for assaying the mitochondrial permeability transition pore (MPTP). ..
  46. doi request reprint Assay of Ca2+ transport by VDAC1 reconstituted into liposomes
    Danya Ben-Hail
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Cold Spring Harb Protoc 2014:195-8. 2014
    ..Despite the disadvantage of needing radiolabeled molecules, this system is highly desirable when the transport properties of noncharged molecules and/or active transporters are studied. ..
  47. doi request reprint Assays of mitochondrial Ca2+ transport and Ca2+ efflux via the MPTP
    Danya Ben-Hail
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Cold Spring Harb Protoc 2014:199-201. 2014
    ..Here, we present a protocol for measuring Ca(2+) transport and release in isolated mitochondria. ..