Research Topics
Genomes and Genes | Varda Shoshan-BarmatzSummaryAffiliation: Ben-Gurion University of the Negev Country: Israel Publications
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Publications
VDAC, a multi-functional mitochondrial protein regulating cell life and deathVarda Shoshan-Barmatz
Department of Life Sciences, and the National Institute for Biotechnology in the Negev NIBN, Ben Gurion University, Beer Sheva, Israel
Mol Aspects Med 31:227-85. 2010..This review also provides insight into the potential of VDAC1 as a rational target for new therapeutics...
Mitochondrial VDAC1: function in cell life and death and a target for cancer therapyV Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Curr Med Chem 19:714-35. 2012..These and other functions point to VDAC1 as being a rational target for the development of a new generation of therapeutics...- Retina expresses a novel variant of the ryanodine receptorVarda Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
Eur J Neurosci 26:3113-25. 2007..The restricted localization of the outer segment RyR to the disk rim suggests that its activation mechanism involves a coupling between retinal RyR and the cGMP-gated channel... - Uncovering the role of VDAC in the regulation of cell life and deathVarda Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
J Bioenerg Biomembr 40:183-91. 2008.... - Key regions of VDAC1 functioning in apoptosis induction and regulation by hexokinaseVarda Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
Biochim Biophys Acta 1787:421-30. 2009..Displacing HK, serving as the 'guardian of the mitochondrion', from its binding site on VDAC1 may thus be exploited as an approach to cancer therapy... - The voltage-dependent anion channel (VDAC): function in intracellular signalling, cell life and cell deathV Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
Curr Pharm Des 12:2249-70. 2006..This review provides insights into the central role of VDAC in cell life and death and emphasizes its function in the regulation of mitochondria-mediated apoptosis and, thereby, its potential as a rational target for new therapeutics... - Hexokinase-I protection against apoptotic cell death is mediated via interaction with the voltage-dependent anion channel-1: mapping the site of bindingSalah Abu-Hamad
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
J Biol Chem 283:13482-90. 2008..Finally, these findings suggest that interference with the binding of HK-I to mitochondria by VDAC1-derived peptides may offer a novel strategy by which to potentiate the efficacy of conventional chemotherapeutic agents... - An N-terminal nucleotide-binding site in VDAC1: involvement in regulating mitochondrial functionGalit Yehezkel
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
J Cell Physiol 212:551-61. 2007..Finally, involvement of the VDAC1-NBS in the control of mitochondrial ATP synthesis, cell growth and viability is discussed... - Voltage-dependent anion channel 1-based peptides interact with hexokinase to prevent its anti-apoptotic activityLaetitia Arzoine
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel 84105
J Biol Chem 284:3946-55. 2009..Moreover, VDAC1-based peptides interfering with HK-mediated anti-apoptotic activity may potentiate the efficacy of conventional chemotherapeutic agents... 
In self-defence: hexokinase promotes voltage-dependent anion channel closure and prevents mitochondria-mediated apoptotic cell deathHeftsi Azoulay-Zohar
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Biochem J 377:347-55. 2004....- Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1Adrian Israelson
Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
Cell Calcium 43:196-204. 2008..Our results thus clearly indicate that RuR protects against cell death via a direct interaction with VDAC1 to inhibit cytochrome c release and subsequent cell death... - Apoptosis is regulated by the VDAC1 N-terminal region and by VDAC oligomerization: release of cytochrome c, AIF and Smac/DiabloVarda Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Biochim Biophys Acta 1797:1281-91. 2010.... - The VDAC1 N-terminus is essential both for apoptosis and the protective effect of anti-apoptotic proteinsSalah Abu-Hamad
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel
J Cell Sci 122:1906-16. 2009..These results show VDAC1 to be a component of the apoptosis machinery and offer new insight into the mechanism of cytochrome c release and how anti-apoptotic proteins regulate apoptosis and promote tumor cell survival... - VDAC1 cysteine residues: topology and function in channel activity and apoptosisLior Aram
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
Biochem J 427:445-54. 2010.... - Mediation of the antiapoptotic activity of Bcl-xL protein upon interaction with VDAC1 proteinNir Arbel
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
J Biol Chem 287:23152-61. 2012..These findings suggest that interfering with Bcl-xL binding to the mitochondria by VDAC1-based peptides may serve to induce apoptosis in cancer cells and to potentiate the efficacy of conventional chemotherapeutic agents... - The voltage-dependent anion channel: characterization, modulation, and role in mitochondrial function in cell life and deathVarda Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
Cell Biochem Biophys 39:279-92. 2003.... - The expression level of the voltage-dependent anion channel controls life and death of the cellSalah Abu-Hamad
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Proc Natl Acad Sci U S A 103:5787-92. 2006.... - Novel ryanodine-binding properties in mammalian retinaVarda Shoshan-Barmatz
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Int J Biochem Cell Biol 37:1681-95. 2005..Its presence in retina suggests that this protein might play a role in controlling intracellular Ca2+ concentration... - Voltage-dependent anion channel 1-based peptides interact with Bcl-2 to prevent antiapoptotic activityNir Arbel
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
J Biol Chem 285:6053-62. 2010..Moreover, these findings suggest that interfering with the binding of Bcl-2 to mitochondria by VDAC1-based peptides may serve to potentiate the efficacy of conventional chemotherapeutic agents... - New fluorescent reagents specific for Ca(2+)-binding proteinsDanya Ben-Hail
Department of Life Sciences and the NIBN, Ben Gurion University, Beer Sheva 84105, Israel
Biochem Biophys Res Commun 426:158-64. 2012..Such reagents will assist in characterizing Ca(2+)-binding proteins, thereby facilitating better understanding of the function of Ca(2+) as a key bio-regulator... - Dominant-negative VDAC1 mutants reveal oligomeric VDAC1 to be the active unit in mitochondria-mediated apoptosisAsaf Mader
Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Biochem J 429:147-55. 2010..These results suggest that E72Q-VDAC1 has a dominant-negative effect and implies that VDAC1 homo-oligomerization, involving intermolecular interactions, might be involved in the apoptotic process... - VDAC, a multi-functional mitochondrial protein as a pharmacological targetVarda Shoshan-Barmatz
Department of Life Sciences and the NIBN, Ben Gurion University, Beer Sheva, Israel
Mitochondrion 12:24-34. 2012..Additionally, we provide insight into the potential of VDAC1 as a rational target for novel therapeutics... - Azido ruthenium: a new photoreactive probe for calcium-binding proteinsAdrian Israelson
Department of Life Sciences, The Zlotowski Center for Neuroscience and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Nat Protoc 1:111-7. 2006..The preparation of AzRu takes approximately 2-3 days... - Localization of the voltage-dependent anion channel-1 Ca2+-binding sitesAdrian Israelson
Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
Cell Calcium 41:235-44. 2007..Furthermore, AzRu protection against apoptosis involves interaction with these residues. Photoreactive AzRu represents an important tool for identifying novel Ca(2+)-binding proteins and localizing their Ca(2+)-binding sites... - Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosisNurit Keinan
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
Mol Cell Biol 30:5698-709. 2010..Thus, targeting the oligomeric status of VDAC, and hence apoptosis, offers a therapeutic strategy for combating cancers and neurodegenerative diseases... - Structure-based analysis of VDAC1: N-terminus location, translocation, channel gating and association with anti-apoptotic proteinsShay Geula
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
Biochem J 444:475-85. 2012..N-terminal mobility may thus contribute to channel gating and interaction with anti-apoptotic proteins... - Downregulation of voltage-dependent anion channel-1 expression by RNA interference prevents cancer cell growth in vivoInbar Koren
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva, Israel
Cancer Biol Ther 9:1046-52. 2010..Taken together, the results of this study demonstrate, for the first time, the anticancer therapeutic potential of VDAC1 downregulation by means of shRNA... - Subcellular localization of VDAC in mitochondria and ER in the cerebellumVarda Shoshan-Barmatz
Department of Life Sciences and Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, P O Box 653, Beer Sheva 84105, Israel
Biochim Biophys Acta 1657:105-14. 2004.... - Glutamate interacts with VDAC and modulates opening of the mitochondrial permeability transition poreDan Gincel
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
J Bioenerg Biomembr 36:179-86. 2004..The interaction of L-glutamate with VDAC and its inhibition of VDAC's channel activity and PTP opening suggest that glutamate may also act as an intracellular messenger in the mitochondria-mediated apoptotic pathway... - Structure-based analysis of VDAC1 protein: defining oligomer contact sitesShay Geula
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
J Biol Chem 287:2179-90. 2012..Dissection of VDAC1 dimerization/oligomerization as presented here provides structural insight into the oligomeric status of cellular VDAC1 under physiological and apoptotic conditions... - NCLX is an essential component of mitochondrial Na+/Ca2+ exchangeRaz Palty
Department of Physiology, and Zlotowski Center for Neuroscience, Ben Gurion University of Negev, Beer Sheva 84105, Israel
Proc Natl Acad Sci U S A 107:436-41. 2010..Taken together, our results converge to the conclusion that NCLX is the long-sought mitochondrial Na(+)/Ca(2+) exchanger... - Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesiclesRan Zalk
Department of Life Sciences, National Institute for Biotechnology, Negev and Zlotowski Center for Neuroscience, Ben Gurion University, Life Sciences Building 40, 84105 Beer Sheva, Israel
FEBS Lett 580:5894-8. 2006..These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems... - Nucleotide-binding sites in the voltage-dependent anion channel: characterization and localizationGalit Yehezkel
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
J Biol Chem 281:5938-46. 2006..Thus, the presence of two or more nucleotide-binding sites in VDAC is suggested, and their possible function in the control of VDAC activity, and, thereby, of outer mitochondrial membrane permeability is discussed... - ATP-binding sites in brain p97/VCP (valosin-containing protein), a multifunctional AAA ATPaseRan Zalk
Department of Life Sciences and Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
Biochem J 374:473-80. 2003..Conformational changes induced by an alternating site mechanism for ATP binding are suggested as a molecular mechanism for coupling ATP binding to the diverse activities of the AAA family... - A photoactivable probe for calcium binding proteinsAdrian Israelson
Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva 84713, Israel
Chem Biol 12:1169-78. 2005..AzRu is a photoreactive reagent that provides an approach for identification of Ca2+ binding proteins, characterization of their binding sites, and exploration of new Ca2+-dependent processes... - Oligomeric states of the voltage-dependent anion channel and cytochrome c release from mitochondriaRan Zalk
Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Biochem J 386:73-83. 2005..Thus the results indicate that VDAC exists in a dynamic equilibrium between dimers and tetramers and suggest that oligomeric VDAC may be involved in mitochondria-mediated apoptosis... - Retinal voltage-dependent anion channel: characterization and cellular localizationDan Gincel
Department of Life Sciences and Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel
Invest Ophthalmol Vis Sci 43:2097-104. 2002..CONCLUSIONS: These results suggest that VDAC is involved in PTP activity and/or regulation and thus is an important player in retinal degeneration associated with PTP-mediated mitochondrial dysfunction... - The synaptic vesicle protein synaptophysin: purification and characterization of its channel activityDan Gincel
Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
Biophys J 83:3223-9. 2002..The availability of large amounts of purified synaptophysin and of its characteristic channel properties might help to establish the role of synaptophysin in synaptic transmission...