R D Sleator

Summary

Affiliation: University College Cork
Country: Ireland

Publications

  1. ncbi request reprint Food reformulations for improved health: A potential risk for microbial food safety?
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, College Road, Cork, Ireland
    Med Hypotheses 69:1323-4. 2007
  2. pmc Role for HtrA in stress induction and virulence potential in Listeria monocytogenes
    Helena M Stack
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 71:4241-7. 2005
  3. ncbi request reprint Gut osmolarity: a key environmental cue initiating the gastrointestinal phase of Listeria monocytogenes infection?
    Roy D Sleator
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Ireland
    Med Hypotheses 69:1090-2. 2007
  4. ncbi request reprint New frontiers in probiotic research
    R D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Lett Appl Microbiol 46:143-7. 2008
  5. ncbi request reprint A role for translational control in listerial osmoregulation and strain variation?
    Roy D Sleator
    Foodborne Pathog Dis 4:395-6. 2007
  6. pmc Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract
    Debbie Watson
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    BMC Microbiol 8:176. 2008
  7. pmc Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice
    Michelle Cronin
    Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland
    BMC Microbiol 8:161. 2008
  8. ncbi request reprint Patho-biotechnology; using bad bugs to make good bugs better
    Roy D Sleator
    Alimentary Pharmabiotic Centre, Cork, Ireland
    Sci Prog 90:1-14. 2007
  9. ncbi request reprint 'Bioengineered Bugs' - a patho-biotechnology approach to probiotic research and applications
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, College Road, Cork, Ireland
    Med Hypotheses 70:167-9. 2008
  10. doi request reprint Molecular analysis of the microbial food safety implications of food reformulations for improved health
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Foodborne Pathog Dis 5:499-504. 2008

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Food reformulations for improved health: A potential risk for microbial food safety?
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, College Road, Cork, Ireland
    Med Hypotheses 69:1323-4. 2007
    ..Thus, a better understanding of the microbiological food safety issues associated with product reformulation for increased health are required...
  2. pmc Role for HtrA in stress induction and virulence potential in Listeria monocytogenes
    Helena M Stack
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 71:4241-7. 2005
    ..Our findings suggest that L. monocytogenes lmo0292 encodes an HtrA-like serine protease that is not part of the classical heat shock response but is involved in stress responses and virulence...
  3. ncbi request reprint Gut osmolarity: a key environmental cue initiating the gastrointestinal phase of Listeria monocytogenes infection?
    Roy D Sleator
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Ireland
    Med Hypotheses 69:1090-2. 2007
    ....
  4. ncbi request reprint New frontiers in probiotic research
    R D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Lett Appl Microbiol 46:143-7. 2008
    ....
  5. ncbi request reprint A role for translational control in listerial osmoregulation and strain variation?
    Roy D Sleator
    Foodborne Pathog Dis 4:395-6. 2007
  6. pmc Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract
    Debbie Watson
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    BMC Microbiol 8:176. 2008
    ..Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS) organism Lactococcus lactis NZ9000...
  7. pmc Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice
    Michelle Cronin
    Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland
    BMC Microbiol 8:161. 2008
    ..Herein we describe, for the first time, the development of a non-invasive luciferase-based reporter system for real-time tracking of Bifidobacterium species in vivo...
  8. ncbi request reprint Patho-biotechnology; using bad bugs to make good bugs better
    Roy D Sleator
    Alimentary Pharmabiotic Centre, Cork, Ireland
    Sci Prog 90:1-14. 2007
    ..This approach shows promise for the design of more technologically robust and effective probiotic cultures with improved biotechnological and clinical applications as well as the development of novel vaccine and drug delivery platforms...
  9. ncbi request reprint 'Bioengineered Bugs' - a patho-biotechnology approach to probiotic research and applications
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, College Road, Cork, Ireland
    Med Hypotheses 70:167-9. 2008
    ....
  10. doi request reprint Molecular analysis of the microbial food safety implications of food reformulations for improved health
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Foodborne Pathog Dis 5:499-504. 2008
    ..Herein we review the most recent advances in our understanding of how microbial pathogens adapt to changes in the food composition, and how this information may ultimately be used for the design of effective pathogen control measures...
  11. ncbi request reprint Engineered pharmabiotics with improved therapeutic potential
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Hum Vaccin 4:271-4. 2008
    ....
  12. doi request reprint Metagenomics
    R D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Lett Appl Microbiol 47:361-6. 2008
    ..In this respect, metagenomics has and will continue to play an essential part in the new and evolving area of microbial systems biology...
  13. ncbi request reprint Carnitine enhances the growth of Listeria monocytogenes in infant formula at 7 degrees C
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, College Road, Cork, Ireland
    J Food Prot 72:1293-5. 2009
    ..monocytogenes mutant in formula containing carnitine that was stored at 7 degrees C. Thus, addition of carnitine to baby formula may pose a potential food safety risk...
  14. doi request reprint A role for proline synthesis and transport in Listeria monocytogenes barotolerance
    K M Considine
    Department of Microbiology, University College Cork, Cork, Ireland
    J Appl Microbiol 110:1187-94. 2011
    ..To assess the contribution of proline biosynthesis to listerial barotolerance...
  15. pmc Analysis of the role of OpuC, an osmolyte transport system, in salt tolerance and virulence potential of Listeria monocytogenes
    R D Sleator
    Department of Microbiology and National Food Biotechnology Centre, University College, Cork, Ireland
    Appl Environ Microbiol 67:2692-8. 2001
    ..Altering OpuC resulted in a significant reduction in the ability of Listeria to colonize the upper small intestine and cause subsequent systemic infection following peroral inoculation...
  16. pmc Mutations in the listerial proB gene leading to proline overproduction: effects on salt tolerance and murine infection
    R D Sleator
    Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 67:4560-5. 2001
    ..However, our results suggest that as previously described for proline auxotrophy, proline hyperproduction has no apparent impact on the virulence potential of Listeria...
  17. ncbi request reprint Betaine and carnitine uptake systems in Listeria monocytogenes affect growth and survival in foods and during infection
    R D Sleator
    Department of Microbiology, Biosciences Institute, University College, Cork, Ireland
    J Appl Microbiol 95:839-46. 2003
    ..To establish the relative importance of the osmo- and cryoprotective compounds glycine betaine and carnitine, and their transporters, for listerial growth and survival, in foods and during infection...
  18. pmc Identification and disruption of BetL, a secondary glycine betaine transport system linked to the salt tolerance of Listeria monocytogenes LO28
    R D Sleator
    Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 65:2078-83. 1999
    ..monocytogenes. This mutant is also unable to withstand concentrations of salt as high as can the BetL+ parent, signifying the role of the transporter in Listeria osmotolerance...
  19. pmc Identification and disruption of the proBA locus in Listeria monocytogenes: role of proline biosynthesis in salt tolerance and murine infection
    R D Sleator
    Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 67:2571-7. 2001
    ....
  20. ncbi request reprint Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003
    Vivien M Sheehan
    Department of Microbiology, University College Cork, Ireland
    Microbiology 153:3563-71. 2007
    ..breve UCC2003-BetL(+) exhibited significantly lower levels of systemic infection compared to the control strain following oral inoculation with Listeria monocytogenes...
  21. pmc Specific osmolyte transporters mediate bile tolerance in Listeria monocytogenes
    Debbie Watson
    Alimentary Pharmabiotic Centre, Department of Microbiology, University College Cork, Western Rd, Cork, Ireland
    Infect Immun 77:4895-904. 2009
    ..This study therefore demonstrates a previously unrecognized function for osmolyte uptake systems in bile tolerance in L. monocytogenes...
  22. pmc Development of a novel oral vaccine against Mycobacterium avium paratuberculosis and Johne disease: a patho-biotechnological approach
    C Johnston
    Cork Institute of Technology, Bishopstown, Cork, Ireland
    Bioeng Bugs 1:155-63. 2010
    ....
  23. pmc Contribution of three bile-associated loci, bsh, pva, and btlB, to gastrointestinal persistence and bile tolerance of Listeria monocytogenes
    Maire Begley
    Department of Microbiology, University College Cork, College Road, Cork, Ireland
    Infect Immun 73:894-904. 2005
    ..Finally, animal (murine) studies revealed an important role for both bsh and btlB in the intestinal persistence of L. monocytogenes...
  24. pmc Heterologous expression of BetL, a betaine uptake system, enhances the stress tolerance of Lactobacillus salivarius UCC118
    Vivien M Sheehan
    Department of Microbiology, University College, Cork, Ireland
    Appl Environ Microbiol 72:2170-7. 2006
    ..The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy...
  25. ncbi request reprint Patho-biotechnology: using bad bugs to do good things
    Roy D Sleator
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College, Cork, Ireland
    Curr Opin Biotechnol 17:211-6. 2006
    ..monocytogenes an ideal model organism for demonstrating the patho-biotechnology concept...
  26. doi request reprint The interaction between Listeria monocytogenes and the host gastrointestinal tract
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Microbiology 155:2463-75. 2009
    ..Here we review some of the exciting recent developments in the analysis of the interaction between L. monocytogenes and the host gastrointestinal tract...
  27. ncbi request reprint Bacterial osmoadaptation: the role of osmolytes in bacterial stress and virulence
    Roy D Sleator
    Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
    FEMS Microbiol Rev 26:49-71. 2002
    ..Finally, we examine the current knowledge on the role of these osmostress responsive systems in contributing to the virulence potential of a number of pathogenic bacteria...
  28. pmc A postgenomic appraisal of osmotolerance in Listeria monocytogenes
    Roy D Sleator
    Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland
    Appl Environ Microbiol 69:1-9. 2003
  29. ncbi request reprint A PrfA-regulated bile exclusion system (BilE) is a novel virulence factor in Listeria monocytogenes
    Roy D Sleator
    Department of Microbiology, University College, Cork, Ireland
    Mol Microbiol 55:1183-95. 2005
    ..Thus, this novel bile exclusion locus bilE, coordinately regulated by sigmaB and PrfA, represents a new and important virulence factor in L. monocytogenes...
  30. pmc Transcriptional regulation and posttranslational activity of the betaine transporter BetL in Listeria monocytogenes are controlled by environmental salinity
    Roy D Sleator
    Department of Microbiology and Alimentary Pharmabiotic Centre, University College, Cork, Ireland
    J Bacteriol 185:7140-4. 2003
    ..In addition, BetL was activated rapidly (half-life, 2 min) in response to an osmotic upshift imposed by adding 2% NaCl to 50 mM potassium phosphate buffer...
  31. pmc Role for compatible solutes glycine betaine and L-carnitine in listerial barotolerance
    Mary Smiddy
    Department of Food and Nutritional Sciences, University College, Cork, Ireland
    Appl Environ Microbiol 70:7555-7. 2004
    ..008 to 0.02% with added L-carnitine (5 mM) and to 0.05% with added betaine (5 mM). Furthermore, listerial cells incapable of transporting compatible solutes fail to adapt to high pressure at elevated osmolarity...
  32. doi request reprint Novel listerial genetic loci conferring enhanced barotolerance in Escherichia coli
    K M Considine
    Department of Microbiology, University College Cork, Cork, Ireland
    J Appl Microbiol 110:618-30. 2011
    ..To identify Listeria monocytogenes genes with a role in high-pressure (HP) resistance...
  33. doi request reprint Detection and characterisation of group A rotavirus in asymptomatic piglets in southern Ireland
    P J Collins
    Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, Ireland
    Arch Virol 155:1247-59. 2010
    ..By sequence analysis of the VP8* fragment of the VP4, the Irish viruses were characterised as P[6], P[7], P[13], P[13]/[22], P[26] and P[32]...
  34. doi request reprint High-pressure processing--effects on microbial food safety and food quality
    Kiera M Considine
    Department of Microbiology, University College Cork, Ireland
    FEMS Microbiol Lett 281:1-9. 2008
    ..Herein, we discuss the effects of high-pressure processing on microbial food safety and, to a lesser degree, food quality...
  35. pmc Rational design of improved pharmabiotics
    Roy D Sleator
    Alimentary Pharmabiotic Centre, University College Cork, Ireland
    J Biomed Biotechnol 2009:275287. 2009
    ....
  36. pmc Molecular and physiological analysis of the role of osmolyte transporters BetL, Gbu, and OpuC in growth of Listeria monocytogenes at low temperatures
    Henrike H Wemekamp-Kamphuis
    Laboratory of Food Microbiology, Wageningen University, Wageningen Centre for Food Sciences, Wageningen, The Netherlands
    Appl Environ Microbiol 70:2912-8. 2004
    ..Here, we conclude that betaine and carnitine transport upon low-temperature exposure is mediated via three osmolyte transporters, BetL, Gbu, and OpuC...
  37. ncbi request reprint Designer probiotics: a potential therapeutic for Clostridium difficile?
    Roy D Sleator
    J Med Microbiol 57:793-4. 2008
  38. doi request reprint Battle of the bugs
    Roy D Sleator
    Science 321:1294-5. 2008
  39. pmc Multiple deletions of the osmolyte transporters BetL, Gbu, and OpuC of Listeria monocytogenes affect virulence and growth at high osmolarity
    Henrike H Wemekamp-Kamphuis
    Laboratory of Food Microbiology, Wageningen University, The Netherlands
    Appl Environ Microbiol 68:4710-6. 2002
    ..Indeed, for LO28BCG, no accumulated betaine was found, but carnitine was accumulated in this strain up to 600 micro mol/g (dry weight) of cells, indicating the presence of a possible fourth osmolyte transporter...