Research Topics
Species | Jeremy C SimpsonSummaryAffiliation: University College Dublin Country: Ireland Publications
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Publications
Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathwayJeremy C Simpson
School of Biology and Environmental Science and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland
Nat Cell Biol 14:764-74. 2012..This work provides an important resource for an integrative understanding of global cellular organization and regulation of the secretory pathway in mammalian cells...
Screening the secretion machinery: High throughput imaging approaches to elucidate the secretory pathwayJeremy C Simpson
School of Biology and Environmental Science and Conway Institute of Biomolecular and Biomedical Research, University College Dublin UCD, Dublin 4, Ireland
Semin Cell Dev Biol 20:903-9. 2009..This review highlights how techniques such as high throughput subcellular localisation, RNA interference screening and automated microscopy are now being harnessed to provide new information about secretory pathway function...
Quantifying size-dependent interactions between fluorescently labeled polystyrene nanoparticles and mammalian cellsJuan A Varela
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland
J Nanobiotechnology 10:39. 2012..Due to the novelty of these applications and to ensure their success, a precise characterization of the interactions between NPs and cells is essential...
A method for improved clustering and classification of microscopy images using quantitative co-localization coefficientsVasanth R Singan
School of Biology and Environmental Science and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Belfield, Ireland
BMC Res Notes 5:281. 2012..However, there are currently very few image analysis options able to effectively discriminate proteins with apparently similar distributions in cells, despite this information being important for protein characterization...
Quantitative image analysis approaches for probing Rab GTPase localization and function in mammalian cellsVasanth R Singan
School of Biology and Environmental Science and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
Biochem Soc Trans 40:1389-93. 2012..We give specific emphasis to the importance of the quantitative nature of these recent studies and comment on their potential applicability to a high-throughput format...
High-speed imaging of Rab family small GTPases reveals rare events in nanoparticle trafficking in living cellsPeter Sandin
Centre for BioNano Interactions, School of Chemistry and Chemical Biology and UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
ACS Nano 6:1513-21. 2012....
Cationic nanoparticles induce caspase 3-, 7- and 9-mediated cytotoxicity in a human astrocytoma cell lineMariana G Bexiga
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland
Nanotoxicology 5:557-67. 2011..Together these results suggest that amine-modified polystyrene nanoparticles can mediate cell death through an apoptotic mechanism mediated by damage to the mitochondria...
Dual channel rank-based intensity weighting for quantitative co-localization of microscopy imagesVasanth R Singan
1School of Medicine and Medical Science, University College Dublin, Belfield, Dublin 4, Ireland
BMC Bioinformatics 12:407. 2011..Furthermore, this choice of whether co-occurrence or correlation is the best approach for quantifying co-localization remains controversial...
