David Sheehan

Summary

Affiliation: University College Cork
Country: Ireland

Publications

  1. ncbi request reprint Redox proteomics in the blue mussel Mytilus edulis: carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stress
    Brian McDonagh
    Proteomics Research Group, Environmental Research Institute, University College Cork, Ireland
    Aquat Toxicol 79:325-33. 2006
  2. doi request reprint Online homology modelling as a means of bridging the sequence-structure gap
    David Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Bioeng Bugs 2:299-305. 2011
  3. ncbi request reprint Redox proteomics in study of kidney-associated hypertension: new insights to old diseases
    David Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Antioxid Redox Signal 17:1560-70. 2012
  4. ncbi request reprint Effects of seasonality on xenobiotic and antioxidant defence mechanisms of bivalve molluscs
    D Sheehan
    Department of Biochemistry, University College Cork, Ireland
    Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 123:193-9. 1999
  5. ncbi request reprint Towards structural biology in the genus Mytilus: on-line homology modeling with Geno3D
    D Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
    Mar Environ Res 62:S411-4. 2006
  6. ncbi request reprint The potential of proteomics for providing new insights into environmental impacts on human health
    David Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Rev Environ Health 22:175-94. 2007
  7. pmc Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily
    D Sheehan
    Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Biochem J 360:1-16. 2001
  8. ncbi request reprint Detection of redox-based modification in two-dimensional electrophoresis proteomic separations
    David Sheehan
    Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Biochem Biophys Res Commun 349:455-62. 2006
  9. doi request reprint Proteomic profiling of perturbed protein sulfenation in renal medulla of the spontaneously hypertensive rat
    Raymond Tyther
    Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
    J Proteome Res 9:2678-87. 2010
  10. doi request reprint Covalent selection of the thiol proteome on activated thiol sepharose: a robust tool for redox proteomics
    Wentao Hu
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Talanta 80:1569-75. 2010

Collaborators

  • Brian McDonagh
  • John O'Halloran
  • Mark G J Hartl
  • Kenneth A Dawson
  • John Davenport
  • Johanna Gardeström
  • Michael Kilemade
  • K England
  • M J Smith
  • C E Mothersill
  • P A Kroon
  • Andreas Prevodnik
  • Karl Lilja
  • Iseult Lynch
  • Maria J Bebianno
  • Tina Elfwing
  • Sara Tedesco
  • Wentao Hu
  • Hugh Doyle
  • Raymond Tyther
  • Julian Blasco
  • Shane McGoldrick
  • Louis Charles Rainville
  • Siobhán M O'Sullivan
  • Vera Dowling
  • Grégoire Herzog
  • M Kiely
  • Gareth Redmond
  • Ahmad Ahmeda
  • Edward Johns
  • Suze Chora
  • S Tedesco
  • D A Shilling
  • Michèle Roméo
  • Vera A Dowling
  • Sharon Ní Shúilleabháin
  • Nora O'Brien
  • Geraldine M Walsh
  • Frank N A M van Pelt
  • Colm Lyons
  • Grainne Darmody
  • Sharon Lynch
  • Sarah Culloty
  • B M Coughlan
  • Darragh Carolan
  • Sonia Ramirez-Garcia
  • Ana Coelho Varela
  • Daniela Iacopino
  • Irene O'Donovan
  • Sarah Keane
  • G J Speijers
  • Roberto Faedda
  • J Gry
  • J C Larsen
  • Amandine Roger
  • L J Black
  • M Kapsokefalou
  • P C Hollman
  • Goffredo Petrone
  • Anne O'Keefe
  • Catherine Keane
  • José Antonio Bárcena
  • J Plumb
  • Santa Olga Cacciola
  • Damien W M Arrigan
  • P Finglas
  • H Doyle
  • Mireille Starita-Geribaldi
  • G Redmond
  • R Tyther
  • F Shanahan
  • H P Redmond
  • E G Kavanagh
  • Roberta F Colman
  • Pascal C Hoarau
  • Ronan M McCarthy
  • Frank van Pelt
  • Melissa A Vargo
  • Tommie V McCarthy
  • Nora M O'Brien
  • Maria Davoren
  • Fiona M Lyng
  • Anthony Kinsella
  • Sarah O'Neill
  • Niamh Moran
  • Michael Tedengren
  • N M O'Brien
  • S J O'Reilly
  • C Morthersill
  • F N A M Van Pelt

Detail Information

Publications45

  1. ncbi request reprint Redox proteomics in the blue mussel Mytilus edulis: carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stress
    Brian McDonagh
    Proteomics Research Group, Environmental Research Institute, University College Cork, Ireland
    Aquat Toxicol 79:325-33. 2006
    ..In digestive gland, this latter ratio was higher than in gill and increased in response to menadione and CdCl(2). Ubiquitination may provide a marker for acute onset of oxidative stress in bivalves...
  2. doi request reprint Online homology modelling as a means of bridging the sequence-structure gap
    David Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Bioeng Bugs 2:299-305. 2011
    ..1mvo), the phosphorylation domain of an outer membrane protein PhoP from Bacillus subtilis, will be described...
  3. ncbi request reprint Redox proteomics in study of kidney-associated hypertension: new insights to old diseases
    David Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Antioxid Redox Signal 17:1560-70. 2012
    ..In comparison with other organs, kidney has an unusually wide range of oxidative status, ranging from the well-perfused cortex to near-anoxic medulla...
  4. ncbi request reprint Effects of seasonality on xenobiotic and antioxidant defence mechanisms of bivalve molluscs
    D Sheehan
    Department of Biochemistry, University College Cork, Ireland
    Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 123:193-9. 1999
    ..Where such molecules show seasonal variation, however, this should be incorporated into interpretation of biomonitoring studies by the use of appropriate controls...
  5. ncbi request reprint Towards structural biology in the genus Mytilus: on-line homology modeling with Geno3D
    D Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
    Mar Environ Res 62:S411-4. 2006
    ..Analysis suggests that they are structurally plausible with low RMSDs relative to targets. Ramachandran analysis suggests a small percentage of disallowed dihedral angles (in the range 0-6.86% for 15 of 17 models)...
  6. ncbi request reprint The potential of proteomics for providing new insights into environmental impacts on human health
    David Sheehan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Rev Environ Health 22:175-94. 2007
    ..Proteomics will continue to be an important toolkit to help address the growing environmental threat posed by nanoparticles and endocrine disrupting agents...
  7. pmc Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily
    D Sheehan
    Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Biochem J 360:1-16. 2001
    ..The implications of this classification with regard to the evolution of GSTs are discussed...
  8. ncbi request reprint Detection of redox-based modification in two-dimensional electrophoresis proteomic separations
    David Sheehan
    Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Biochem Biophys Res Commun 349:455-62. 2006
    ..This article describes key methodologies now available for identification of redox-based modifications in proteins separated by 2D SDS-PAGE...
  9. doi request reprint Proteomic profiling of perturbed protein sulfenation in renal medulla of the spontaneously hypertensive rat
    Raymond Tyther
    Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
    J Proteome Res 9:2678-87. 2010
    ..Enhanced sulfenation may represent perturbed redox signaling in SHR medulla, or simply enhanced ROS generation...
  10. doi request reprint Covalent selection of the thiol proteome on activated thiol sepharose: a robust tool for redox proteomics
    Wentao Hu
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Talanta 80:1569-75. 2010
    ..This revealed that some proteins present in controls were absent in pro-oxidant-treated extracts which we attribute to thiol oxidation. ATS has significant potential in enrichment for -SH-containing proteins in redox proteomics...
  11. doi request reprint Shotgun redox proteomics in sub-proteomes trapped on functionalised beads: Identification of proteins targeted by oxidative stress
    Wentao Hu
    Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
    Mar Environ Res 69:S25-7. 2010
    ..This method may be generally useful in ecotoxicology for identification of oxidative stress targets...
  12. doi request reprint Oxidative stress and toxicity of gold nanoparticles in Mytilus edulis
    Sara Tedesco
    Environmental Research Institute of University College Cork, Cork, Ireland
    Aquat Toxicol 100:178-86. 2010
    ..This study suggests that M. edulis is a suitable model animal for environmental toxicology studies of nanoparticles...
  13. ncbi request reprint Protein carbonylation and heat shock response in Ruditapes decussatus following p,p'-dichlorodiphenyldichloroethylene (DDE) exposure: a proteomic approach reveals that DDE causes oxidative stress
    Vera Dowling
    Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
    Aquat Toxicol 77:11-8. 2006
    ..These results demonstrate for the first time that DDE causes tissue-specific formation of reactive oxygen species in clams...
  14. ncbi request reprint Carbonylation and glutathionylation of proteins in the blue mussel Mytilus edulis detected by proteomic analysis and Western blotting: Actin as a target for oxidative stress
    Brian McDonagh
    Proteomics Research Group, Department of Biochemistry and Environmental Research Institute, University College Cork, Cork, Ireland
    Aquat Toxicol 73:315-26. 2005
    ..edulis. Actin is a major target for both glutathionylation and carbonylation under oxidative stress conditions...
  15. doi request reprint Exposure of the blue mussel, Mytilus edulis, to gold nanoparticles and the pro-oxidant menadione
    Sara Tedesco
    Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
    Comp Biochem Physiol C Toxicol Pharmacol 151:167-74. 2010
    ..Our results suggest that GNP causes a modest level of oxidative stress sufficient to oxidize thiols in glutathione and proteins but without causing lipid peroxidation or induction of thioredoxin reductase activity...
  16. doi request reprint Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-Sepharose
    Wentao Hu
    Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
    Anal Biochem 398:245-53. 2010
    ..coli. ATS provides a convenient and rapid way to select thiol-containing proteins...
  17. doi request reprint Gold nanoparticles and oxidative stress in the blue mussel, Mytilus edulis
    Sara Tedesco
    Proteomics Research Laboratory, Department of Biochemistry, University College Cork, Cork, Ireland
    Methods Mol Biol 1028:197-203. 2013
    ..After scanning for IAF-associated fluorescence, gels are stained with colloidal coomassie. A ratio of fluorescence to protein stain is calculated revealing that thiol oxidation increases with GNP treatment...
  18. ncbi request reprint Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive rats
    Raymond Tyther
    Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
    Proteomics 7:4555-64. 2007
    ..Our results suggest that kidney medulla is subject to enhanced nitrosative and oxidative stress, and that resulting protein modifications may contribute to the progression of hypertension...
  19. ncbi request reprint Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L
    Mark G J Hartl
    Environmental Research Institute, University College Cork, Ireland
    Mar Environ Res 64:191-208. 2007
    ..maximus is a suitable sentinel species for the assessment of moderately contaminated sediments and therefore allows for the further development of this model for future, ecologically relevant, field studies...
  20. ncbi request reprint Proteomics as a route to identification of toxicity targets in environmental toxicology
    Vera A Dowling
    Environmental Research Institute and Proteomics Research Group, Department of Biochemistry, University College, Cork, Ireland
    Proteomics 6:5597-604. 2006
    ..This review summarises the main approaches currently used in this area and assesses the potential of proteomics for identification of novel toxicity targets...
  21. ncbi request reprint Effect of oxidative stress on protein thiols in the blue mussel Mytilus edulis: proteomic identification of target proteins
    Brian McDonagh
    Proteomics Research Group, Department of Biochemistry and Environmental Research Institute, University College Cork, Cork, Ireland
    Proteomics 7:3395-403. 2007
    ..Since many environmental pollutants cause oxidative stress, studies on PDI and structural proteins may be particularly relevant to understanding toxicity in this popular sentinel species...
  22. ncbi request reprint Glutathione transferase-like proteins encoded in genomes of yeasts and fungi: insights into evolution of a multifunctional protein superfamily
    Shane McGoldrick
    Department of Biochemistry and Analytical and Biological Chemistry Research Facility, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    FEMS Microbiol Lett 242:1-12. 2005
    ..We suggest that GST-like sequences are widespread in fungi, participating in a wide range of functions. They probably evolved by a process similar to domain "shuffling"...
  23. doi request reprint EuroFIR eBASIS: application for health claims submissions and evaluations
    M Kiely
    School of Food and Nutritional Sciences, University College Cork, Ireland
    Eur J Clin Nutr 64:S101-7. 2010
    ..This report evaluates the eBASIS biological effects database in the preparation and evaluation of health claims dossiers...
  24. ncbi request reprint Chemical modification at subunit 1 of rat kidney Alpha class glutathione transferase with 2,3,5,6-tetrachloro-1,4-benzoquinone: close structural connectivity between glutathione conjugation activity and non-substrate ligand binding
    Siobhán M O'Sullivan
    Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Biochem Pharmacol 71:1629-36. 2006
    ..Homology modelling of rGSTA1-1 modified at Cys-17 of one subunit revealed only modest structural perturbations in the second subunit and tends to exclude global structural effects...
  25. ncbi request reprint Variability of heat shock proteins and glutathione S-transferase in gill and digestive gland of blue mussel, Mytilus edulis
    Colm Lyons
    Environmental Research Institute, University College Cork, Ireland
    Mar Environ Res 56:585-97. 2003
    ..10- and 3-fold, respectively, at Tjärnö compared to the other sites which we attribute to exposure to more widely fluctuating pollution levels...
  26. ncbi request reprint The 110kDa glutathione transferase of Yarrowia lipolytica is encoded by a homologue of the TEF3 gene from Saccharomyces cerevisiae: cloning, expression, and homology modeling of the recombinant protein
    Shane McGoldrick
    Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Biochem Biophys Res Commun 337:1125-32. 2005
    ..Partial tryptic digestion released two fragments of M(r) 22 and 18kDa, which we interpret as N- and C-terminal domains. Homology modeling confirmed that the N-terminal domain of Y. lipolytica TEF4 encodes a GST-like protein...
  27. ncbi request reprint Redox proteomics in the mussel, Mytilus edulis
    B McDonagh
    Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
    Mar Environ Res 62:S101-4. 2006
    ..Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms...
  28. ncbi request reprint Effects of oxidative stress on protein thiols and disulphides in Mytilus edulis revealed by proteomics: actin and protein disulphide isomerase are redox targets
    B McDonagh
    Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
    Mar Environ Res 66:193-5. 2008
    ..Protein disulphide isomerase (PDI) was labelled both as -SH and -S-S-, underlining its involvement in the redox status of the animal. A glutathione transferase (GST P1-1) forms an inter-chain disulphide bridge in response to menadione...
  29. ncbi request reprint Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: a redox proteomic investigation
    Wentao Hu
    Environmental Research Institute and Department of Biochemistry, University College Cork, Ireland
    Chemosphere 108:289-99. 2014
    ....
  30. ncbi request reprint Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna
    Louis Charles Rainville
    Proteomics Research Group, School of Biochemistry and Cell Biology, University College Cork, Western Gateway Building 3 99, Western Road, Cork, Ireland
    Analyst 139:1678-86. 2014
    ....
  31. ncbi request reprint Fast protein liquid chromatography
    David Sheehan
    Department of Biochemistry and Analytical and Biological Chemistry Research Facility, University College Cork, Mardyke, Ireland
    Methods Mol Biol 244:253-8. 2004
  32. doi request reprint Ion-transfer voltammetric behavior of protein digests at liquid/liquid interfaces
    Grégoire Herzog
    Tyndall National Institute, Lee Maltings, University College Cork, Cork, Ireland
    Anal Chem 82:258-64. 2010
    ....
  33. doi request reprint Effects of contaminated sediment from Cork Harbour, Ireland on the cytochrome P450 system of turbot
    M Kilemade
    Department of Zoology, Ecology and Plant Science, University College Cork, Ireland
    Ecotoxicol Environ Saf 72:747-55. 2009
    ..P450 activities were generally significantly higher than those exposed to reference site sediment. Liver was the most responsive tissue with significantly greater P450 activities compared with gill and intestinal tissues...
  34. ncbi request reprint Implications of seasonal priming and reproductive activity on the interpretation of Comet assay data derived from the clam, Tapes semidecussatus Reeves 1864, exposed to contaminated sediments
    M G J Hartl
    Environmental Research Institute, University College Cork, Ireland
    Mar Environ Res 57:295-310. 2004
    ..Furthermore, the degree of DNA damage in clams exposed to contaminated sediments was higher in autumn and winter compared to spring and summer, suggesting an effect of seasonal priming...
  35. ncbi request reprint An assessment of the pollutant status of surficial sediment in Cork Harbour in the South East of Ireland with particular reference to polycyclic aromatic hydrocarbons
    M Kilemade
    Environmental Research Institute, University College Cork, Cork, Ireland
    Mar Pollut Bull 49:1084-96. 2004
    ..A similar pattern was observed with the other contaminants however, these compounds, with the exception of the heavy metals, all tended to be detected at concentrations on or below detection limits...
  36. ncbi request reprint Genotoxicity of field-collected inter-tidal sediments from Cork Harbor, Ireland, to juvenile turbot (Scophthalmus maximus L.) as measured by the Comet assay
    M F Kilemade
    Environmental Research Institute, University College, Cork, Ireland
    Environ Mol Mutagen 44:56-64. 2004
    ..Results from the study indicate a relationship between the presence of genotoxicants in sediment and DNA damage. This finding was encouraging with regard to the potential use of the Comet assay as part of a marine biomonitoring strategy...
  37. ncbi request reprint Gold nanoparticles and oxidative stress in Mytilus edulis
    S Tedesco
    Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
    Mar Environ Res 66:131-3. 2008
    ..2DE gels were performed on gill proteins (menadione; GNP/menadione). Our results reveal that GNP may induce oxidative stress...
  38. ncbi request reprint The genetics of osteoporosis: vitamin D receptor gene polymorphisms and circulating osteocalcin in healthy Irish adults
    D Sheehan
    Department of Nutrition, University College Cork, Ireland
    Ir J Med Sci 170:54-7. 2001
    ..There is conflicting evidence of linkage between the VDR genotype and differences in bone mineral density. No such study has been carried out on a resident Irish population...
  39. ncbi request reprint Detecting genotoxicity using the Comet assay following chronic exposure of Manila clam Tapes semidecussatus to polluted estuarine sediments
    B M Coughlan
    Environmental Research Institute, University College Cork, Cork, Ireland
    Mar Pollut Bull 44:1359-65. 2002
    ..We conclude that the Comet assay is a useful tool for the detection of DNA damage in clams chronically exposed to polluted sediments...
  40. pmc Salmonella typhimurium stimulation combined with tumour-derived heat shock proteins induces potent dendritic cell anti-tumour responses in a murine model
    D A Shilling
    Alimentary Pharmabiotic Centre, National University of Ireland, Cork, Ireland
    Clin Exp Immunol 149:109-16. 2007
    ..Dendritic cells primed with a proinflammatory bacterial stimulus and tumour-associated antigens induce a protective anti-tumour immune response in this murine model...
  41. ncbi request reprint Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic Sea
    Andreas Prevodnik
    School of Life Sciences, Sodertorn University College, S 141 89 Huddinge, Sweden
    Aquat Toxicol 82:63-71. 2007
    ....
  42. ncbi request reprint A two-species biomarker model for the assessment of sediment toxicity in the marine and estuarine environment using the comet assay
    Mark G J Hartl
    J Environ Sci Health A Tox Hazard Subst Environ Eng 41:939-53. 2006
    ..This suggests that turbot, rather than clams, are more suitable as a sentinel species for the assessment of genotoxic impact of low-level contamination in aquatic sediments and highlights the need for a two- or multi-species approach...
  43. ncbi request reprint Identification of a multixenobiotic resistance mechanism in primary cultured epidermal cells from Oncorhynchus mykiss and the effects of environmental complex mixtures on its activity
    Sharon Ní Shúilleabháin
    Radiation and Environmental Science Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland
    Aquat Toxicol 73:115-27. 2005
    ..The findings of the functional study also demonstrated that environmental pollutants, which interfere with P-gp function, could be identified using this model...
  44. ncbi request reprint Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus
    Suze Chora
    University of Algarve, CIMA, Faculty of Marine and Environmental Sciences, Campus de Gambelas, Faro, Portugal
    Mar Environ Res 66:95-7. 2008
    ..In this approach, changes in protein structure provide options for affinity selection of sub-proteomes for 2D SDS-PAGE, simplifying the detection of protein biomarkers using proteomic approach...
  45. ncbi request reprint Redox modulation of integrin [correction of integin] alpha IIb beta 3 involves a novel allosteric regulation of its thiol isomerase activity
    Geraldine M Walsh
    Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland
    Biochemistry 43:473-80. 2004
    ..Thus, redox agents simultaneously modulate the thiol isomerase activity of purified alphaIIbbeta3 and its active conformation in intact platelets, suggesting a molecular mechanism for integrin regulation...