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Genomes and Genes | David SheehanSummaryAffiliation: University College Cork Country: Ireland Publications
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Publications
Redox proteomics in the blue mussel Mytilus edulis: carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stressBrian McDonagh
Proteomics Research Group, Environmental Research Institute, University College Cork, Ireland
Aquat Toxicol 79:325-33. 2006..In digestive gland, this latter ratio was higher than in gill and increased in response to menadione and CdCl(2). Ubiquitination may provide a marker for acute onset of oxidative stress in bivalves...
Online homology modelling as a means of bridging the sequence-structure gapDavid Sheehan
Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Bioeng Bugs 2:299-305. 2011..1mvo), the phosphorylation domain of an outer membrane protein PhoP from Bacillus subtilis, will be described...- Redox proteomics in study of kidney-associated hypertension: new insights to old diseasesDavid Sheehan
Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
Antioxid Redox Signal 17:1560-70. 2012..Proteomics approaches have been taken to study of human kidney tissue, serum/plasma, urine, and animal models of hypertension... - Detection of redox-based modification in two-dimensional electrophoresis proteomic separationsDavid Sheehan
Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Biochem Biophys Res Commun 349:455-62. 2006..This article describes key methodologies now available for identification of redox-based modifications in proteins separated by 2D SDS-PAGE... - The potential of proteomics for providing new insights into environmental impacts on human healthDavid Sheehan
Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Rev Environ Health 22:175-94. 2007..Proteomics will continue to be an important toolkit to help address the growing environmental threat posed by nanoparticles and endocrine disrupting agents... - Effects of seasonality on xenobiotic and antioxidant defence mechanisms of bivalve molluscsD Sheehan
Department of Biochemistry, University College Cork, Ireland
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 123:193-9. 1999..Where such molecules show seasonal variation, however, this should be incorporated into interpretation of biomonitoring studies by the use of appropriate controls... - Towards structural biology in the genus Mytilus: on-line homology modeling with Geno3DD Sheehan
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
Mar Environ Res 62:S411-4. 2006..Analysis suggests that they are structurally plausible with low RMSDs relative to targets. Ramachandran analysis suggests a small percentage of disallowed dihedral angles (in the range 0-6.86% for 15 of 17 models)... 
Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamilyD Sheehan
Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Biochem J 360:1-16. 2001..The implications of this classification with regard to the evolution of GSTs are discussed...- Proteomic profiling of perturbed protein sulfenation in renal medulla of the spontaneously hypertensive ratRaymond Tyther
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
J Proteome Res 9:2678-87. 2010..Enhanced sulfenation may represent perturbed redox signaling in SHR medulla, or simply enhanced ROS generation... - Shotgun redox proteomics in sub-proteomes trapped on functionalised beads: Identification of proteins targeted by oxidative stressWentao Hu
Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
Mar Environ Res 69:S25-7. 2010..This method may be generally useful in ecotoxicology for identification of oxidative stress targets... - Covalent selection of the thiol proteome on activated thiol sepharose: a robust tool for redox proteomicsWentao Hu
Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
Talanta 80:1569-75. 2010..This revealed that some proteins present in controls were absent in pro-oxidant-treated extracts which we attribute to thiol oxidation. ATS has significant potential in enrichment for -SH-containing proteins in redox proteomics... - Oxidative stress and toxicity of gold nanoparticles in Mytilus edulisSara Tedesco
Environmental Research Institute of University College Cork, Cork, Ireland
Aquat Toxicol 100:178-86. 2010..This study suggests that M. edulis is a suitable model animal for environmental toxicology studies of nanoparticles... - Carbonylation and glutathionylation of proteins in the blue mussel Mytilus edulis detected by proteomic analysis and Western blotting: Actin as a target for oxidative stressBrian McDonagh
Proteomics Research Group, Department of Biochemistry and Environmental Research Institute, University College Cork, Cork, Ireland
Aquat Toxicol 73:315-26. 2005..edulis. Actin is a major target for both glutathionylation and carbonylation under oxidative stress conditions... - Exposure of the blue mussel, Mytilus edulis, to gold nanoparticles and the pro-oxidant menadioneSara Tedesco
Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
Comp Biochem Physiol C Toxicol Pharmacol 151:167-74. 2010..Our results suggest that GNP causes a modest level of oxidative stress sufficient to oxidize thiols in glutathione and proteins but without causing lipid peroxidation or induction of thioredoxin reductase activity... - Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-SepharoseWentao Hu
Proteomics Research Group, Department of Biochemistry, University College Cork, Cork, Ireland
Anal Biochem 398:245-53. 2010..coli. ATS provides a convenient and rapid way to select thiol-containing proteins... - Protein carbonylation and heat shock response in Ruditapes decussatus following p,p'-dichlorodiphenyldichloroethylene (DDE) exposure: a proteomic approach reveals that DDE causes oxidative stressVera Dowling
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
Aquat Toxicol 77:11-8. 2006..These results demonstrate for the first time that DDE causes tissue-specific formation of reactive oxygen species in clams... - Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus LMark G J Hartl
Environmental Research Institute, University College Cork, Ireland
Mar Environ Res 64:191-208. 2007..maximus is a suitable sentinel species for the assessment of moderately contaminated sediments and therefore allows for the further development of this model for future, ecologically relevant, field studies... - Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive ratsRaymond Tyther
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland
Proteomics 7:4555-64. 2007..Our results suggest that kidney medulla is subject to enhanced nitrosative and oxidative stress, and that resulting protein modifications may contribute to the progression of hypertension... - Proteomics as a route to identification of toxicity targets in environmental toxicologyVera A Dowling
Environmental Research Institute and Proteomics Research Group, Department of Biochemistry, University College, Cork, Ireland
Proteomics 6:5597-604. 2006..This review summarises the main approaches currently used in this area and assesses the potential of proteomics for identification of novel toxicity targets... - Effect of oxidative stress on protein thiols in the blue mussel Mytilus edulis: proteomic identification of target proteinsBrian McDonagh
Proteomics Research Group, Department of Biochemistry and Environmental Research Institute, University College Cork, Cork, Ireland
Proteomics 7:3395-403. 2007..Since many environmental pollutants cause oxidative stress, studies on PDI and structural proteins may be particularly relevant to understanding toxicity in this popular sentinel species... - Glutathione transferase-like proteins encoded in genomes of yeasts and fungi: insights into evolution of a multifunctional protein superfamilyShane McGoldrick
Department of Biochemistry and Analytical and Biological Chemistry Research Facility, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
FEMS Microbiol Lett 242:1-12. 2005..We suggest that GST-like sequences are widespread in fungi, participating in a wide range of functions. They probably evolved by a process similar to domain "shuffling"... - EuroFIR eBASIS: application for health claims submissions and evaluationsM Kiely
School of Food and Nutritional Sciences, University College Cork, Ireland
Eur J Clin Nutr 64:S101-7. 2010..This report evaluates the eBASIS biological effects database in the preparation and evaluation of health claims dossiers... - Variability of heat shock proteins and glutathione S-transferase in gill and digestive gland of blue mussel, Mytilus edulisColm Lyons
Environmental Research Institute, University College Cork, Ireland
Mar Environ Res 56:585-97. 2003..10- and 3-fold, respectively, at Tjärnö compared to the other sites which we attribute to exposure to more widely fluctuating pollution levels... - The 110kDa glutathione transferase of Yarrowia lipolytica is encoded by a homologue of the TEF3 gene from Saccharomyces cerevisiae: cloning, expression, and homology modeling of the recombinant proteinShane McGoldrick
Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Biochem Biophys Res Commun 337:1125-32. 2005..Partial tryptic digestion released two fragments of M(r) 22 and 18kDa, which we interpret as N- and C-terminal domains. Homology modeling confirmed that the N-terminal domain of Y. lipolytica TEF4 encodes a GST-like protein... - Redox proteomics in the mussel, Mytilus edulisB McDonagh
Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Mar Environ Res 62:S101-4. 2006..Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms... - Effects of oxidative stress on protein thiols and disulphides in Mytilus edulis revealed by proteomics: actin and protein disulphide isomerase are redox targetsB McDonagh
Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
Mar Environ Res 66:193-5. 2008..Protein disulphide isomerase (PDI) was labelled both as -SH and -S-S-, underlining its involvement in the redox status of the animal. A glutathione transferase (GST P1-1) forms an inter-chain disulphide bridge in response to menadione... - Chemical modification at subunit 1 of rat kidney Alpha class glutathione transferase with 2,3,5,6-tetrachloro-1,4-benzoquinone: close structural connectivity between glutathione conjugation activity and non-substrate ligand bindingRonan M McCarthy
Proteomics Research Group, Department of Biochemistry, University College Cork, Lee Maltings, Prospect Row, Mardyke, Cork, Ireland
Biochem Pharmacol 71:1629-36. 2006..Homology modelling of rGSTA1-1 modified at Cys-17 of one subunit revealed only modest structural perturbations in the second subunit and tends to exclude global structural effects... - Ion-transfer voltammetric behavior of protein digests at liquid/liquid interfacesGrégoire Herzog
Tyndall National Institute, Lee Maltings, University College Cork, Cork, Ireland
Anal Chem 82:258-64. 2010.... - Fast protein liquid chromatographyDavid Sheehan
Department of Biochemistry and Analytical and Biological Chemistry Research Facility, University College Cork, Mardyke, Ireland
Methods Mol Biol 244:253-8. 2004 - Effects of contaminated sediment from Cork Harbour, Ireland on the cytochrome P450 system of turbotM Kilemade
Department of Zoology, Ecology and Plant Science, University College Cork, Ireland
Ecotoxicol Environ Saf 72:747-55. 2009..P450 activities were generally significantly higher than those exposed to reference site sediment. Liver was the most responsive tissue with significantly greater P450 activities compared with gill and intestinal tissues... - Detecting genotoxicity using the Comet assay following chronic exposure of Manila clam Tapes semidecussatus to polluted estuarine sedimentsB M Coughlan
Environmental Research Institute, University College Cork, Cork, Ireland
Mar Pollut Bull 44:1359-65. 2002..We conclude that the Comet assay is a useful tool for the detection of DNA damage in clams chronically exposed to polluted sediments... - An assessment of the pollutant status of surficial sediment in Cork Harbour in the South East of Ireland with particular reference to polycyclic aromatic hydrocarbonsM Kilemade
Environmental Research Institute, University College Cork, Cork, Ireland
Mar Pollut Bull 49:1084-96. 2004..A similar pattern was observed with the other contaminants however, these compounds, with the exception of the heavy metals, all tended to be detected at concentrations on or below detection limits... - Genotoxicity of field-collected inter-tidal sediments from Cork Harbor, Ireland, to juvenile turbot (Scophthalmus maximus L.) as measured by the Comet assayM F Kilemade
Environmental Research Institute, University College, Cork, Ireland
Environ Mol Mutagen 44:56-64. 2004..Results from the study indicate a relationship between the presence of genotoxicants in sediment and DNA damage. This finding was encouraging with regard to the potential use of the Comet assay as part of a marine biomonitoring strategy... - Salmonella typhimurium stimulation combined with tumour-derived heat shock proteins induces potent dendritic cell anti-tumour responses in a murine modelD A Shilling
Alimentary Pharmabiotic Centre, National University of Ireland, Cork, Ireland
Clin Exp Immunol 149:109-16. 2007..Dendritic cells primed with a proinflammatory bacterial stimulus and tumour-associated antigens induce a protective anti-tumour immune response in this murine model... - Implications of seasonal priming and reproductive activity on the interpretation of Comet assay data derived from the clam, Tapes semidecussatus Reeves 1864, exposed to contaminated sedimentsM G J Hartl
Environmental Research Institute, University College Cork, Ireland
Mar Environ Res 57:295-310. 2004..Furthermore, the degree of DNA damage in clams exposed to contaminated sediments was higher in autumn and winter compared to spring and summer, suggesting an effect of seasonal priming... - The genetics of osteoporosis: vitamin D receptor gene polymorphisms and circulating osteocalcin in healthy Irish adultsD Sheehan
Department of Nutrition, University College Cork, Ireland
Ir J Med Sci 170:54-7. 2001..There is conflicting evidence of linkage between the VDR genotype and differences in bone mineral density. No such study has been carried out on a resident Irish population... - Gold nanoparticles and oxidative stress in Mytilus edulisS Tedesco
Proteomics Research Group, Department of Biochemistry, Environmental Research Institute, University College Cork, Cork, Ireland
Mar Environ Res 66:131-3. 2008..2DE gels were performed on gill proteins (menadione; GNP/menadione). Our results reveal that GNP may induce oxidative stress... - Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic SeaAndreas Prevodnik
School of Life Sciences, Sodertorn University College, S 141 89 Huddinge, Sweden
Aquat Toxicol 82:63-71. 2007.... - Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatusSuze Chora
University of Algarve, CIMA, Faculty of Marine and Environmental Sciences, Campus de Gambelas, Faro, Portugal
Mar Environ Res 66:95-7. 2008..In this approach, changes in protein structure provide options for affinity selection of sub-proteomes for 2D SDS-PAGE, simplifying the detection of protein biomarkers using proteomic approach... - Redox modulation of integrin [correction of integin] alpha IIb beta 3 involves a novel allosteric regulation of its thiol isomerase activityGeraldine M Walsh
Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland
Biochemistry 43:473-80. 2004..Thus, redox agents simultaneously modulate the thiol isomerase activity of purified alphaIIbbeta3 and its active conformation in intact platelets, suggesting a molecular mechanism for integrin regulation... - Identification of a multixenobiotic resistance mechanism in primary cultured epidermal cells from Oncorhynchus mykiss and the effects of environmental complex mixtures on its activitySharon Ní Shúilleabháin
Radiation and Environmental Science Centre, Focas Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland
Aquat Toxicol 73:115-27. 2005..The findings of the functional study also demonstrated that environmental pollutants, which interfere with P-gp function, could be identified using this model... - A two-species biomarker model for the assessment of sediment toxicity in the marine and estuarine environment using the comet assayMark G J Hartl
J Environ Sci Health A Tox Hazard Subst Environ Eng 41:939-53. 2006..This suggests that turbot, rather than clams, are more suitable as a sentinel species for the assessment of genotoxic impact of low-level contamination in aquatic sediments and highlights the need for a two- or multi-species approach...