Michael Freeley

Summary

Affiliation: Trinity College
Country: Ireland

Publications

  1. doi RNAi Screening with Self-Delivering, Synthetic siRNAs for Identification of Genes That Regulate Primary Human T Cell Migration
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland
    J Biomol Screen 20:943-56. 2015
  2. doi The two hit hypothesis: an improved method for siRNA-mediated gene silencing in stimulated primary human T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland Electronic address
    J Immunol Methods 396:116-27. 2013
  3. doi L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland
    J Immunol 188:6357-70. 2012
  4. ncbi Loss of PTEN expression does not contribute to PDK-1 activity and PKC activation-loop phosphorylation in Jurkat leukaemic T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 19:2444-57. 2007
  5. doi Regulation of Protein Kinase C function by phosphorylation on conserved and non-conserved sites
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 23:753-62. 2011
  6. ncbi Stimulus-induced phosphorylation of PKC theta at the C-terminal hydrophobic-motif in human T lymphocytes
    Michael Freeley
    Department of Biochemistry, Royal College of Surgeons in Ireland, 123 St Stephens Green, Dublin 2, Ireland
    Biochem Biophys Res Commun 334:619-30. 2005
  7. doi Analysis of dynamic tyrosine phosphoproteome in LFA-1 triggered migrating T-cells
    Navin K Verma
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Ireland
    J Cell Physiol 226:1489-98. 2011
  8. doi A high-content analysis toolbox permits dissection of diverse signaling pathways for T lymphocyte polarization
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    J Biomol Screen 15:541-55. 2010
  9. ncbi CD44 cross-linking induces protein kinase C-regulated migration of human T lymphocytes
    Aine Fanning
    Department of Clinical Medicine, Trinity College, and Dublin Molecular Medicine Centre, James s Street, Dublin 8, Ireland
    Int Immunol 17:449-58. 2005
  10. ncbi The hepatitis C envelope 2 protein inhibits LFA-1-transduced protein kinase C signaling for T-lymphocyte migration
    Yuri Volkov
    Dublin Molecular Medicine Centre and Department of Clinical Medicine, Trinity Centre College, Ireland
    Gastroenterology 130:482-92. 2006

Collaborators

Detail Information

Publications17

  1. doi RNAi Screening with Self-Delivering, Synthetic siRNAs for Identification of Genes That Regulate Primary Human T Cell Migration
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland
    J Biomol Screen 20:943-56. 2015
    ....
  2. doi The two hit hypothesis: an improved method for siRNA-mediated gene silencing in stimulated primary human T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland Electronic address
    J Immunol Methods 396:116-27. 2013
    ..This new and improved two-hit nucleofection method for siRNA-mediated gene silencing in stimulated primary human T cells will therefore permit the investigation of genes and signalling pathways in the T cell immune response...
  3. doi L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland
    J Immunol 188:6357-70. 2012
    ....
  4. ncbi Loss of PTEN expression does not contribute to PDK-1 activity and PKC activation-loop phosphorylation in Jurkat leukaemic T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 19:2444-57. 2007
    ..We conclude therefore that loss of PTEN expression in Jurkat T cells does not impact on the PDK-1/PKC pathway and that only a subset of kinases, such as PKB/Akt, are perturbed as a consequence PTEN loss...
  5. doi Regulation of Protein Kinase C function by phosphorylation on conserved and non-conserved sites
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 23:753-62. 2011
    ..Here we discuss the role of phosphorylation in controlling PKC function and outline our current understanding of the mechanisms that regulate these phosphorylation sites...
  6. ncbi Stimulus-induced phosphorylation of PKC theta at the C-terminal hydrophobic-motif in human T lymphocytes
    Michael Freeley
    Department of Biochemistry, Royal College of Surgeons in Ireland, 123 St Stephens Green, Dublin 2, Ireland
    Biochem Biophys Res Commun 334:619-30. 2005
    ..However, hydrophobic-motif phosphorylation does not appear to control membrane translocation, suggesting that this site may control other aspects of PKC theta signalling...
  7. doi Analysis of dynamic tyrosine phosphoproteome in LFA-1 triggered migrating T-cells
    Navin K Verma
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Ireland
    J Cell Physiol 226:1489-98. 2011
    ....
  8. doi A high-content analysis toolbox permits dissection of diverse signaling pathways for T lymphocyte polarization
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    J Biomol Screen 15:541-55. 2010
    ..The assay described here may be used in HCA strategies such as RNAi screening assays to elucidate the signaling pathways and molecules that regulate integrin/adhesion receptor-mediated T lymphocyte polarization...
  9. ncbi CD44 cross-linking induces protein kinase C-regulated migration of human T lymphocytes
    Aine Fanning
    Department of Clinical Medicine, Trinity College, and Dublin Molecular Medicine Centre, James s Street, Dublin 8, Ireland
    Int Immunol 17:449-58. 2005
    ..This suggests potential convergence of intracellular signaling pathways induced via CD44 and LFA-1 integrin...
  10. ncbi The hepatitis C envelope 2 protein inhibits LFA-1-transduced protein kinase C signaling for T-lymphocyte migration
    Yuri Volkov
    Dublin Molecular Medicine Centre and Department of Clinical Medicine, Trinity Centre College, Ireland
    Gastroenterology 130:482-92. 2006
    ..The aim of this study was to investigate the effect of the hepatitis C envelope protein E2 on LFA-1-stimulated T-cell migration and macrophage inflammatory protein (MIP-1alpha, MIP-1beta) secretion...
  11. pmc Phosphorylation of Rab5a protein by protein kinase Cϵ is crucial for T-cell migration
    Seow Theng Ong
    From the From the Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 8, Ireland
    J Biol Chem 289:19420-34. 2014
    ..We present a novel mechanism by which a PKCϵ-Rab5a-Rac1 axis regulates cytoskeleton remodeling and T-cell migration, both of which are central for the adaptive immune response. ..
  12. doi Advances in siRNA delivery to T-cells: potential clinical applications for inflammatory disease, cancer and infection
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland
    Biochem J 455:133-47. 2013
    ..We also discuss the potential therapeutic benefits of manipulating T-cells using siRNAs for the treatment of human diseases...
  13. doi Hepatitis C virus targets the T cell secretory machinery as a mechanism of immune evasion
    Danijela Petrovic
    Department of Clinical Medicine and Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland
    Hepatology 53:1846-53. 2011
    ..HCV E2 further inhibited the secretion of other cytokines, including interferon-γ...
  14. doi Candidate Gene Knockdown in Celiac Disease
    Ben Molloy
    Department of Medicine, Institute of Molecular Medicine, Trinity College Dublin, St James s Hospital, Dublin 8, Ireland
    Methods Mol Biol 1326:159-73. 2015
    ..This chapter outlines protocols for examining the effects of RNAi on candidate genes and subsequent changes to migratory phenotype, transmigration, and adhesion. ..
  15. doi Protein kinase C: a regulator of cytoskeleton remodelling and T-cell migration
    Aideen Long
    Department of Clinical Medicine, Trinity College Dublin, Dublin 2, Ireland
    Biochem Soc Trans 42:1490-7. 2014
    ....
  16. ncbi Comment on "PDK1 nucleates T cell receptor-induced signaling complex for NF-kappaB activation"
    Thomas Gruber
    Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Austria
    Science 312:55; author reply 55. 2006
    ..Reports, 1 April 2005, p. 114) that the Thr-538 phosphorylation of PKC is regulated by T cell receptor activation. Other mechanisms, such as autophosphorylation of Thr-219, might orchestrate the cellular function of PKC in T cells...