Michael Freeley

Summary

Affiliation: Trinity College
Country: Ireland

Publications

  1. doi The two hit hypothesis: an improved method for siRNA-mediated gene silencing in stimulated primary human T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland Electronic address
    J Immunol Methods 396:116-27. 2013
  2. ncbi L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland
    J Immunol 188:6357-70. 2012
  3. doi Regulation of Protein Kinase C function by phosphorylation on conserved and non-conserved sites
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 23:753-62. 2011
  4. ncbi Loss of PTEN expression does not contribute to PDK-1 activity and PKC activation-loop phosphorylation in Jurkat leukaemic T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 19:2444-57. 2007
  5. ncbi Stimulus-induced phosphorylation of PKC theta at the C-terminal hydrophobic-motif in human T lymphocytes
    Michael Freeley
    Department of Biochemistry, Royal College of Surgeons in Ireland, 123 St Stephens Green, Dublin 2, Ireland
    Biochem Biophys Res Commun 334:619-30. 2005
  6. ncbi A high-content analysis toolbox permits dissection of diverse signaling pathways for T lymphocyte polarization
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    J Biomol Screen 15:541-55. 2010
  7. doi Analysis of dynamic tyrosine phosphoproteome in LFA-1 triggered migrating T-cells
    Navin K Verma
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Ireland
    J Cell Physiol 226:1489-98. 2011
  8. ncbi CD44 cross-linking induces protein kinase C-regulated migration of human T lymphocytes
    Aine Fanning
    Department of Clinical Medicine, Trinity College, and Dublin Molecular Medicine Centre, James s Street, Dublin 8, Ireland
    Int Immunol 17:449-58. 2005
  9. ncbi The hepatitis C envelope 2 protein inhibits LFA-1-transduced protein kinase C signaling for T-lymphocyte migration
    Yuri Volkov
    Dublin Molecular Medicine Centre and Department of Clinical Medicine, Trinity Centre College, Ireland
    Gastroenterology 130:482-92. 2006
  10. ncbi Phosphorylation of rab5a protein by protein kinase cϵ is crucial for T-cell migration
    Seow Theng Ong
    From the From the Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 8, Ireland
    J Biol Chem 289:19420-34. 2014

Collaborators

Detail Information

Publications14

  1. doi The two hit hypothesis: an improved method for siRNA-mediated gene silencing in stimulated primary human T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland Electronic address
    J Immunol Methods 396:116-27. 2013
    ..This new and improved two-hit nucleofection method for siRNA-mediated gene silencing in stimulated primary human T cells will therefore permit the investigation of genes and signalling pathways in the T cell immune response...
  2. ncbi L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 2, Ireland
    J Immunol 188:6357-70. 2012
    ....
  3. doi Regulation of Protein Kinase C function by phosphorylation on conserved and non-conserved sites
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 23:753-62. 2011
    ..Here we discuss the role of phosphorylation in controlling PKC function and outline our current understanding of the mechanisms that regulate these phosphorylation sites...
  4. ncbi Loss of PTEN expression does not contribute to PDK-1 activity and PKC activation-loop phosphorylation in Jurkat leukaemic T cells
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    Cell Signal 19:2444-57. 2007
    ..We conclude therefore that loss of PTEN expression in Jurkat T cells does not impact on the PDK-1/PKC pathway and that only a subset of kinases, such as PKB/Akt, are perturbed as a consequence PTEN loss...
  5. ncbi Stimulus-induced phosphorylation of PKC theta at the C-terminal hydrophobic-motif in human T lymphocytes
    Michael Freeley
    Department of Biochemistry, Royal College of Surgeons in Ireland, 123 St Stephens Green, Dublin 2, Ireland
    Biochem Biophys Res Commun 334:619-30. 2005
    ..However, hydrophobic-motif phosphorylation does not appear to control membrane translocation, suggesting that this site may control other aspects of PKC theta signalling...
  6. ncbi A high-content analysis toolbox permits dissection of diverse signaling pathways for T lymphocyte polarization
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College, Dublin, Ireland
    J Biomol Screen 15:541-55. 2010
    ..The assay described here may be used in HCA strategies such as RNAi screening assays to elucidate the signaling pathways and molecules that regulate integrin/adhesion receptor-mediated T lymphocyte polarization...
  7. doi Analysis of dynamic tyrosine phosphoproteome in LFA-1 triggered migrating T-cells
    Navin K Verma
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Ireland
    J Cell Physiol 226:1489-98. 2011
    ....
  8. ncbi CD44 cross-linking induces protein kinase C-regulated migration of human T lymphocytes
    Aine Fanning
    Department of Clinical Medicine, Trinity College, and Dublin Molecular Medicine Centre, James s Street, Dublin 8, Ireland
    Int Immunol 17:449-58. 2005
    ..This suggests potential convergence of intracellular signaling pathways induced via CD44 and LFA-1 integrin...
  9. ncbi The hepatitis C envelope 2 protein inhibits LFA-1-transduced protein kinase C signaling for T-lymphocyte migration
    Yuri Volkov
    Dublin Molecular Medicine Centre and Department of Clinical Medicine, Trinity Centre College, Ireland
    Gastroenterology 130:482-92. 2006
    ..The aim of this study was to investigate the effect of the hepatitis C envelope protein E2 on LFA-1-stimulated T-cell migration and macrophage inflammatory protein (MIP-1alpha, MIP-1beta) secretion...
  10. ncbi Phosphorylation of rab5a protein by protein kinase cϵ is crucial for T-cell migration
    Seow Theng Ong
    From the From the Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin 8, Ireland
    J Biol Chem 289:19420-34. 2014
    ..We present a novel mechanism by which a PKCϵ-Rab5a-Rac1 axis regulates cytoskeleton remodeling and T-cell migration, both of which are central for the adaptive immune response. ..
  11. doi Advances in siRNA delivery to T-cells: potential clinical applications for inflammatory disease, cancer and infection
    Michael Freeley
    Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland
    Biochem J 455:133-47. 2013
    ..We also discuss the potential therapeutic benefits of manipulating T-cells using siRNAs for the treatment of human diseases...
  12. doi Hepatitis C virus targets the T cell secretory machinery as a mechanism of immune evasion
    Danijela Petrovic
    Department of Clinical Medicine and Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland
    Hepatology 53:1846-53. 2011
    ..HCV E2 further inhibited the secretion of other cytokines, including interferon-γ...
  13. ncbi Comment on "PDK1 nucleates T cell receptor-induced signaling complex for NF-kappaB activation"
    Thomas Gruber
    Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Austria
    Science 312:55; author reply 55. 2006
    ..Reports, 1 April 2005, p. 114) that the Thr-538 phosphorylation of PKC is regulated by T cell receptor activation. Other mechanisms, such as autophosphorylation of Thr-219, might orchestrate the cellular function of PKC in T cells...