Research Topics
Genomes and Genes | Kay OhlendieckSummaryAffiliation: National University of Ireland Country: Ireland Publications
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Detail Information
Publications
Proteomics of skeletal muscle glycolysisKay Ohlendieck
Muscle Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Biochim Biophys Acta 1804:2089-101. 2010....- Proteomic identification of biomarkers of skeletal muscle disordersKay Ohlendieck
Muscle Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Biomark Med 7:169-86. 2013..These mass spectrometric studies have clearly established skeletal muscle proteomics as a reliable method for the identification of novel indicators of neuromuscular diseases... 
Skeletal muscle proteomics: current approaches, technical challenges and emerging techniquesKay Ohlendieck
Muscle Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Skelet Muscle 1:6. 2011..abstract:..- Proteomics of skeletal muscle differentiation, neuromuscular disorders and fiber agingKay Ohlendieck
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Expert Rev Proteomics 7:283-96. 2010..New muscle markers will be crucial for the development of improved diagnostics, the monitoring of disease progression, evaluation of drug action and the identification of novel therapeutic targets... 
Aging skeletal muscle shows a drastic increase in the small heat shock proteins alphaB-crystallin/HspB5 and cvHsp/HspB7Philip Doran
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Eur J Cell Biol 86:629-40. 2007..These findings indicate that the increase in muscle-specific small heat shock proteins constitutes an essential cellular response to fibre aging and might therefore be a novel therapeutic option to treat sarcopenia of old age...- Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient musclePhilip Doran
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Biochim Biophys Acta 1764:773-85. 2006.... - Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscleKathleen O'Connell
Department of Biology, National University of Ireland, Co Kildare, Ireland
Int J Mol Med 20:145-53. 2007.... - Proteomic profiling of x-linked muscular dystrophyCaroline Lewis
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
J Muscle Res Cell Motil 30:267-9. 2009.... - Lectin-based proteomic profiling of aged skeletal muscle: decreased pyruvate kinase isozyme M1 exhibits drastically increased levels of N-glycosylationKathleen O'Connell
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Eur J Cell Biol 87:793-805. 2008..This agrees with the idea that abnormal post-translational modifications in key metabolic enzymes may be involved in the conversion of aged muscle to slower twitch patterns and a drastic shift to more aerobic-oxidative metabolism... - Drastic increase of myosin light chain MLC-2 in senescent skeletal muscle indicates fast-to-slow fibre transition in sarcopenia of old ageJoan Gannon
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Eur J Cell Biol 88:685-700. 2009.... - Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscleKathleen O'Connell
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Exp Gerontol 43:958-61. 2008..This may trigger abnormal luminal Ca(2+)-buffering and/or decreased plasmalemmal Ca(2+)-removal, which could exacerbate impaired signaling or disturbed intracellular ion balance in aged fibres, thereby causing contractile weakness... - Phosphoproteomic analysis of aged skeletal muscleJoan Gannon
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Int J Mol Med 22:33-42. 2008..This confirms that sarcopenia of old age is a complex neuromuscular pathology that is associated with drastic changes in the abundance and structure of key muscle proteins... - Mass spectrometric identification of dystrophin isoform Dp427 by on-membrane digestion of sarcolemma from skeletal muscleCaroline Lewis
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Anal Biochem 404:197-203. 2010..On-membrane digestion has the potential to develop into the method of choice for studying these classes of proteins, whose presence is otherwise missed by conventional gel electrophoresis-based proteomics... - Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal musclePhilip Doran
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Eur J Biochem 271:3943-52. 2004..The previously described impaired Ca(2+) buffering capacity of the dystrophic sarcoplasmic reticulum is probably caused by a reduction in luminal Ca(2+)-binding proteins, including calsequestrin... - Proteomic profiling of antisense-induced exon skipping reveals reversal of pathobiochemical abnormalities in dystrophic mdx diaphragmPhilip Doran
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Proteomics 9:671-85. 2009..This clearly establishes the exon-skipping approach as a realistic treatment strategy for diminishing diverse downstream alterations in dystrophinopathy... - Proteomic profiling of pathological and aged skeletal muscle fibres by peptide mass fingerprinting (Review)Philip Doran
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Int J Mol Med 19:547-64. 2007..Proteomics is one of the most promising new analytical ways of comparing large muscle protein complements and has the potential to decisively improve modern biochemical and biomedical research into neuromuscular disorders... - Profiling of age-related changes in the tibialis anterior muscle proteome of the mdx mouse model of dystrophinopathySteven Carberry
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
J Biomed Biotechnol 2012:691641. 2012..The identification of novel indicators of progressive muscular dystrophy might be useful for the establishment of a muscle subtype-specific biomarker signature of dystrophinopathy... - Mass spectrometric characterization of the sarcoplasmic reticulum from rabbit skeletal muscle by on-membrane digestionLisa Staunton
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Protein Pept Lett 19:252-63. 2012..Hence, on-membrane digestion is highly suitable as the method of choice for studying integral and high-molecular-mass proteins in proteomic studies... - Opposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysisPhilip Doran
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Proteomics 8:364-77. 2008.... - Proteomic profiling of non-obese type 2 diabetic skeletal muscleEdel Mullen
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Int J Mol Med 25:445-58. 2010..Reliable markers could be used for improving diagnostics, monitoring of disease progression and therapeutic evaluations... - Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscleKathleen O'Connell
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Proteomics 9:5509-24. 2009..This suggests that mitochondrial markers are reliable candidates that should be included in the future establishment of a biomarker signature of skeletal muscle aging... - Proteome analysis of the dystrophin-deficient MDX diaphragm reveals a drastic increase in the heat shock protein cvHSPPhilip Doran
Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland
Proteomics 6:4610-21. 2006..Stress response appears to be an important cellular mechanism in dystrophic muscle and may be exploitable as a new approach to counteract muscle degeneration... - Differential expression of the fast skeletal muscle proteome following chronic low-frequency stimulationPamela Donoghue
Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland
Biochim Biophys Acta 1752:166-76. 2005.... - Proteomics reveals drastic increase of extracellular matrix proteins collagen and dermatopontin in the aged mdx diaphragm model of Duchenne muscular dystrophySteven Carberry
Department of Biology, National University of Ireland, Maynooth, Kildare, Republic of Ireland
Int J Mol Med 30:229-34. 2012..These proteomic findings establish the aged mdx diaphragm as an excellent model system for studying secondary effects of dystrophin deficiency in skeletal muscle tissue... - Proteomic analysis of dystrophic muscleCaroline Lewis
Department of Biology, National University of Ireland Maynooth, Maynooth, Co Kildare, Ireland
Methods Mol Biol 798:357-69. 2012.... - Proteomic profiling of naturally protected extraocular muscles from the dystrophin-deficient mdx mouseCaroline Lewis
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Biochem Biophys Res Commun 396:1024-9. 2010..Interestingly, the adaptive mechanisms that cause the sparing of extraocular fibers seem to be closely linked to an enhanced cellular stress response... - Identification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscleLisa Staunton
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Mol Biosyst 7:2480-9. 2011.... - Proteomic analysis of muscle affected by motor neuron degeneration: the wobbler mouse model of amyotrophic lateral sclerosisLisa Staunton
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Biochem Biophys Res Commun 406:595-600. 2011..The establishment of novel disease-specific biomarkers of motor neuron disease might be helpful in the design of improved diagnostic tools and the identification of novel therapeutic targets... - Skeletal muscle tissue from the Goto-Kakizaki rat model of type-2 diabetes exhibits increased levels of the small heat shock protein Hsp27Edel Mullen
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Mol Med Rep 4:229-36. 2011..Increased levels of molecular chaperones suggest considerably enhanced cellular stress levels in diabetic muscle fibres... - Mass spectrometry-based proteomic analysis of middle-aged vs. aged vastus lateralis reveals increased levels of carbonic anhydrase isoform 3 in senescent human skeletal muscleLisa Staunton
Department of Biology, National University of Ireland, Maynooth, Kildare, Ireland
Int J Mol Med 30:723-33. 2012..Increased levels of CA are indicative of an increased demand of CO₂-removal in senescent muscle, and also suggest age-related fibre type shifting to slower-contracting muscles during human aging... - Proteomic profiling of animal models mimicking skeletal muscle disordersPhilip Doran
Department of Biology, National University of Ireland, Maynooth Co Kildare, Ireland
Proteomics Clin Appl 1:1169-84. 2007.... - Oligomerisation of sarcoplasmic reticulum Ca2+-ATPase monomers from skeletal muscleDaniela Schreiber
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Protein Pept Lett 14:219-26. 2007..This suggests a preferential complex formation via homo-dimerization, rather than interactions with established oligomeric structures... - Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle AgingLisa Staunton
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
J Aging Res 2011:908035. 2011.... - Early effects of denervation on Ca(2+)-handling proteins in skeletal musclePamela Donoghue
Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
Int J Mol Med 13:767-72. 2004..Hence, changes in muscle activity appear to have a profound effect on the abundance and isoform expression pattern of Ca(2+)-handling elements... - Drastic reduction in the luminal Ca2+ -binding proteins calsequestrin and sarcalumenin in dystrophin-deficient cardiac muscleJames Lohan
Department of Biology, National University of Ireland, Maynooth, Co. Kildare
Biochim Biophys Acta 1689:252-8. 2004..Abnormal Ca2+ -handling appears to play a major role in the molecular pathogenesis of the cardiac involvement in X-linked muscular dystrophy... - Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile ProteinsCaroline Lewis
Department of Biology, National University of Ireland, Maynooth, Kildare, Ireland
J Biomed Biotechnol 2010:648501. 2010..Hence, the lack of cardiac dystrophin appears to trigger a generally perturbed protein expression pattern in the MDX heart, affecting especially energy metabolism and contractile proteins... - Extraction of membrane proteinsKay Ohlendieck
Department of Biology, National University of Ireland, Maynooth
Methods Mol Biol 244:283-93. 2004 - Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophyPaul Dowling
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland
Biochem J 379:479-88. 2004..This confirms the idea that abnormal Ca2+ cycling is involved in Ca2+-induced myonecrosis. Hence, manipulating disturbed Ca2+ handling might represent new modes of abolishing proteolytic degradation in muscular dystrophy... - Ca2+-regulatory muscle proteins in the alcohol-fed ratKay Ohlendieck
Department of Biology, National University of Ireland, Maynooth, Co Kildare
Metabolism 52:1102-12. 2003..0950). We conclude that upregulation of SERCA1 protein and Ca(2+)-ATPase activity may be an adaptive mechanism and/or a contributory process in the pathology of alcohol-induced muscle disease...