Shyam Sundar

Summary

Country: India

Publications

  1. ncbi request reprint Cure of antimony-unresponsive Indian post-kala-azar dermal leishmaniasis with oral miltefosine
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    Trans R Soc Trop Med Hyg 100:698-700. 2006
  2. pmc Rapid, noninvasive diagnosis of visceral leishmaniasis in India: comparison of two immunochromatographic strip tests for detection of anti-K39 antibody
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    J Clin Microbiol 44:251-3. 2006
  3. ncbi request reprint Treatment of visceral leishmaniasis
    Shyam Sundar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    Expert Opin Pharmacother 6:2821-9. 2005
  4. pmc Availability of miltefosine for the treatment of kala-azar in India
    Shyam Sundar
    Institute of Medical Sciences, Banaras Hindu University, 6 SK Gupta Nagar, Lanka, Varanasi 221 005, India
    Bull World Health Organ 83:394-5. 2005
  5. doi request reprint Molecular and serological markers of Leishmania donovani infection in healthy individuals from endemic areas of Bihar, India
    Pankaj Srivastava
    Infectious Disease Research Laboratory, Banaras Hindu University, Varanasi, India
    Trop Med Int Health 18:548-54. 2013
  6. doi request reprint Comparison of short-course multidrug treatment with standard therapy for visceral leishmaniasis in India: an open-label, non-inferiority, randomised controlled trial
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Lancet 377:477-86. 2011
  7. doi request reprint Post-kala-azar dermal leishmaniasis in visceral leishmaniasis-endemic communities in Bihar, India
    Rudra Pratap Singh
    Banaras Hindu University, Varanasi, India Institute of Tropical Medicine, Antwerp, Belgium Barcelona Centre for International Health Research CRESIB, Hospital Clínic Universitat de Barcelona, Barcelona, Spain Geneva University Hospitals, Geneva, Switzerland
    Trop Med Int Health 17:1345-8. 2012
  8. pmc Genetic and functional evaluation of the role of DLL1 in susceptibility to visceral leishmaniasis in India
    Sanjana Mehrotra
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, OS 221 005, India
    Infect Genet Evol 12:1195-201. 2012
  9. pmc Genetic and functional evaluation of the role of CXCR1 and CXCR2 in susceptibility to visceral leishmaniasis in north-east India
    Sanjana Mehrotra
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    BMC Med Genet 12:162. 2011
  10. doi request reprint Single-dose liposomal amphotericin B for visceral leishmaniasis in India
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    N Engl J Med 362:504-12. 2010

Collaborators

Detail Information

Publications125 found, 100 shown here

  1. ncbi request reprint Cure of antimony-unresponsive Indian post-kala-azar dermal leishmaniasis with oral miltefosine
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    Trans R Soc Trop Med Hyg 100:698-700. 2006
    ..Oral miltefosine appears to be an important alternative for the treatment of PKDL in India and confirmatory studies in controlled clinical trials are needed...
  2. pmc Rapid, noninvasive diagnosis of visceral leishmaniasis in India: comparison of two immunochromatographic strip tests for detection of anti-K39 antibody
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    J Clin Microbiol 44:251-3. 2006
    ..yielded comparable results for symptomatic infection and identified apparent subclinical infection in 15 to 32% of healthy residents in a region where visceral leishmaniasis is highly endemic...
  3. ncbi request reprint Treatment of visceral leishmaniasis
    Shyam Sundar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    Expert Opin Pharmacother 6:2821-9. 2005
    ..A shift from monotherapy to multi-drug combinations of short courses delivered at no or affordable cost, through directly observed therapy, seems to be the only way to develop the treatment of this disease...
  4. pmc Availability of miltefosine for the treatment of kala-azar in India
    Shyam Sundar
    Institute of Medical Sciences, Banaras Hindu University, 6 SK Gupta Nagar, Lanka, Varanasi 221 005, India
    Bull World Health Organ 83:394-5. 2005
  5. doi request reprint Molecular and serological markers of Leishmania donovani infection in healthy individuals from endemic areas of Bihar, India
    Pankaj Srivastava
    Infectious Disease Research Laboratory, Banaras Hindu University, Varanasi, India
    Trop Med Int Health 18:548-54. 2013
    ..Recent epidemiological reports indicate that asymptomatic human infections with Leishmania donovani, the causative agent of visceral leishmaniasis or Kala-azar (KA), occur frequently in India. We explored markers of infection...
  6. doi request reprint Comparison of short-course multidrug treatment with standard therapy for visceral leishmaniasis in India: an open-label, non-inferiority, randomised controlled trial
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Lancet 377:477-86. 2011
    ..Improved treatment approaches are needed for visceral leishmaniasis. We assessed the efficacy and safety of three potential short-course combination treatments compared with the standard monotherapy in India...
  7. doi request reprint Post-kala-azar dermal leishmaniasis in visceral leishmaniasis-endemic communities in Bihar, India
    Rudra Pratap Singh
    Banaras Hindu University, Varanasi, India Institute of Tropical Medicine, Antwerp, Belgium Barcelona Centre for International Health Research CRESIB, Hospital Clínic Universitat de Barcelona, Barcelona, Spain Geneva University Hospitals, Geneva, Switzerland
    Trop Med Int Health 17:1345-8. 2012
    ..The prevalence of confirmed PKDL cases was 4.4 per 10 000 individuals and 7.8 if probable cases were also considered. The clinical history and treatment of the post-kala-azar dermal leishmaniasis cases are discussed. ..
  8. pmc Genetic and functional evaluation of the role of DLL1 in susceptibility to visceral leishmaniasis in India
    Sanjana Mehrotra
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, OS 221 005, India
    Infect Genet Evol 12:1195-201. 2012
    ....
  9. pmc Genetic and functional evaluation of the role of CXCR1 and CXCR2 in susceptibility to visceral leishmaniasis in north-east India
    Sanjana Mehrotra
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    BMC Med Genet 12:162. 2011
    ..Variants at CXCR1 and CXCR2 have been associated with susceptibility to cutaneous and mucocutaneous leishmaniasis in Brazil. Here we investigate the role of CXCR1/CXCR2 in visceral leishmaniasis (VL) in India...
  10. doi request reprint Single-dose liposomal amphotericin B for visceral leishmaniasis in India
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    N Engl J Med 362:504-12. 2010
    ..We wanted to determine whether the efficacy of a single infusion of liposomal amphotericin B was inferior to conventional parenteral therapy, consisting of 15 alternate-day infusions of amphotericin B deoxycholate...
  11. pmc A multiattribute utility evaluation of different methods for the detection of enteric protozoa causing diarrhea in AIDS patients
    Lekha Tuli
    Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    BMC Microbiol 10:11. 2010
    ..Thus, a diagnostic protocol was devised for rapid, sensitive and cost effective identification of the opportunistic enteric protozoa...
  12. pmc CD8 T cell exhaustion in human visceral leishmaniasis
    Shalini Gautam
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
    J Infect Dis 209:290-9. 2014
    ..We suggest CD8 T cells are driven to anergy/exhaustion in human VL, which affect their ability to contribute to protective immune responses. ..
  13. pmc Comparative evaluation of blood and serum samples in rapid immunochromatographic tests for visceral leishmaniasis
    Dinesh Kumar
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
    J Clin Microbiol 51:3955-9. 2013
    ..We conclude that whole blood is an adequate alternative for serum in RDTs for VL, with sensitivities and specificities comparable to those obtained in serum samples, provided that the test kit is of overall good quality. ..
  14. pmc In vitro susceptibility of Leishmania donovani to miltefosine in Indian visceral leishmaniasis
    Vijay Kumar Prajapati
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, UP, India Unit of Epidemiology and Control of Tropical Diseases, Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India Unit of Molecular Parasitology, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Department of Biomedical Sciences, Antwerp University, Antwerp, Belgium
    Am J Trop Med Hyg 89:750-4. 2013
    ..Our work highlights the need for careful monitoring of MIL susceptibility for implementation in national VL elimination programs...
  15. doi request reprint Leishmaniasis: an update of current pharmacotherapy
    Shyam Sundar
    Banaras Hindu University, Institute of Medical Sciences, Department of Medicine, Varanasi 221 005, India
    Expert Opin Pharmacother 14:53-63. 2013
    ..The treatment of VL is challenging. The duration of treatment is long, and drugs are toxic thereby needing monitoring and hospitalization...
  16. pmc Reassessment of immune correlates in human visceral leishmaniasis as defined by cytokine release in whole blood
    Om Prakash Singh
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Clin Vaccine Immunol 19:961-6. 2012
    ....
  17. doi request reprint Efficacy of miltefosine in the treatment of visceral leishmaniasis in India after a decade of use
    Shyam Sundar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Clin Infect Dis 55:543-50. 2012
    ..Its unrestricted use has raised concern about its continued effectiveness. This study evaluates the efficacy and safety of miltefosine for the treatment of VL after a decade of use in India...
  18. pmc IL-10 neutralization promotes parasite clearance in splenic aspirate cells from patients with visceral leishmaniasis
    Shalini Gautam
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    J Infect Dis 204:1134-7. 2011
    ..These findings provide direct support for targeting IL-10 as an approach to therapy in human VL...
  19. ncbi request reprint Oral miltefosine treatment in children with mild to moderate Indian visceral leishmaniasis
    Shyam Sundar
    Institutes of Medical Sciences, Banaras Hindu University, 6 SK Gupta Nagar, Varanasi 221005, India
    Pediatr Infect Dis J 22:434-8. 2003
    ..Because approximately one-half of the visceral leishmaniasis patients worldwide are children, we performed a Phase I/II dose ranging study in the pediatric population in India...
  20. doi request reprint Antileishmanial activity of nano-amphotericin B deoxycholate
    Krishna Das Manandhar
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    J Antimicrob Chemother 62:376-80. 2008
    ..The aim of the present study was to compare the efficacy of a nano form of amphotericin B deoxycholate with that of conventional amphotericin B deoxycholate for the treatment of visceral leishmaniasis...
  21. pmc Targeted killing of Leishmania donovani in vivo and in vitro with amphotericin B attached to functionalized carbon nanotubes
    Vijay Kumar Prajapati
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    J Antimicrob Chemother 66:874-9. 2011
    ..This study describes the antileishmanial efficacy of the novel drug formulation of amphotericin B (AmB) attached to functionalized carbon nanotubes (f-CNTs) and compares it with AmB...
  22. pmc Leishmania specific CD4 T cells release IFNγ that limits parasite replication in patients with visceral leishmaniasis
    Rajiv Kumar
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India Department of Immunology and Infection, Queensland Institute of Medical Research, Herston, Queensland, Australia
    PLoS Negl Trop Dis 8:e3198. 2014
    ..Most importantly, blockade of IFNγ in ex-vivo splenic aspirate cultures demonstrated that despite the progressive nature of their disease, the endogenous IFNγ produced in patients with active VL serves to limit parasite growth. ..
  23. pmc Seasonal variation in the prevalence of sand flies infected with Leishmania donovani
    Puja Tiwary
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
    PLoS ONE 8:e61370. 2013
    ..Estimation of the infectivity in the vector population, collected in different seasons, may be useful to better understanding the transmission dynamics of VL as well as to plan vector control measures...
  24. ncbi request reprint Amphotericin B colloidal dispersion for the treatment of Indian visceral leishmaniasis
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Clin Infect Dis 42:608-13. 2006
    ..However, cost is a major constraint in the use of lipid formulations of amphotericin B. Amphotericin B colloidal dispersion (ABCD), which is a less expensive lipid formulation, has not been tested for the treatment of VL in India...
  25. doi request reprint Short-course paromomycin treatment of visceral leishmaniasis in India: 14-day vs 21-day treatment
    Shyam Sundar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Clin Infect Dis 49:914-8. 2009
    ..Treatment of visceral leishmaniasis (VL) is far from satisfactory. There is an urgent need for a therapy that is efficacious, safe, affordable, and of short duration...
  26. ncbi request reprint Amphotericin B treatment for Indian visceral leishmaniasis: response to 15 daily versus alternate-day infusions
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Banaras Hindu University, Institute of Medical Sciences, Varanasi, India
    Clin Infect Dis 45:556-61. 2007
    ..daily administration) and dose (1 vs. 0.75 mg/kg) and to determine whether the duration of amphotericin B treatment in Bihar, India, can be shortened to 15 days...
  27. doi request reprint New treatment approach in Indian visceral leishmaniasis: single-dose liposomal amphotericin B followed by short-course oral miltefosine
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Banaras Hindu University, Institute of Medical Sciences, Varanasi, India
    Clin Infect Dis 47:1000-6. 2008
    ....
  28. ncbi request reprint Oral miltefosine for the treatment of Indian visceral leishmaniasis
    Shyam Sundar
    Kala azar Medical Research Center, Banaras Hindu University, Varanasi, India
    Trans R Soc Trop Med Hyg 100:S26-33. 2006
    ..5 mg/kg daily for 28 days) was established with a cure rate (94%) similar to that seen in adults. Miltefosine is the first oral antileishmanial drug with a high degree of safety and efficacy for the treatment of VL...
  29. pmc Persistence of Leishmania donovani antibodies in past visceral leishmaniasis cases in India
    Kamlesh Gidwani
    Banaras Hindu University, Varanasi, India
    Clin Vaccine Immunol 18:346-8. 2011
    ..Antibody titers remained high for an extended period of time in past cases of VL. These results highlight the need to carefully elicit the history of patients with VL symptoms...
  30. pmc In vitro antileishmanial drug susceptibility of clinical isolates from patients with Indian visceral leishmaniasis--status of newly introduced drugs
    Vijay Kumar Prajapati
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 87:655-7. 2012
    ....
  31. pmc Identification and characterization of a novel Leishmania donovani antigen for serodiagnosis of visceral leishmaniasis
    Subodh Kumar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University Varanasi, India
    Am J Trop Med Hyg 86:601-5. 2012
    ..Further analysis showed that it is a member of the heat shock protein family of 70 kDa, designated as BHUP1, and has great potential in the diagnosis of VL...
  32. pmc An oral formulation of amphotericin B attached to functionalized carbon nanotubes is an effective treatment for experimental visceral leishmaniasis
    Vijay Kumar Prajapati
    Department of Medicine, Infectious Disease Research Laboratory, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    J Infect Dis 205:333-6. 2012
    ..In this study, using the hamster model, we have shown that this novel formulation of AmB can be administered orally, resulting in 99% inhibition of parasite growth following a 5-day course at 15 mg/kg body weight...
  33. pmc Serological markers of sand fly exposure to evaluate insecticidal nets against visceral leishmaniasis in India and Nepal: a cluster-randomized trial
    Kamlesh Gidwani
    Banaras Hindu University, Varanasi, India
    PLoS Negl Trop Dis 5:e1296. 2011
    ..This study quantified the effect of LNs on exposure to the sand fly vector of VL in India and Nepal during a two year community intervention trial...
  34. ncbi request reprint Injectable paromomycin for Visceral leishmaniasis in India
    Shyam Sundar
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
    N Engl J Med 356:2571-81. 2007
    ..Safe, effective, and affordable new therapies are needed. We conducted a randomized, controlled, phase 3 open-label study comparing paromomycin, an aminoglycoside, with amphotericin B, the present standard of care in Bihar, India...
  35. pmc Unusual case of resistance to amphotericin B in visceral leishmaniasis in a region in India where leishmaniasis is not endemic
    Pankaj Srivastava
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    J Clin Microbiol 49:3088-91. 2011
    ..Overexpression and underexpression of proteins were observed in the 65- to 80-kDa range and at 20 kDa, respectively...
  36. pmc Quantitative PCR in epidemiology for early detection of visceral leishmaniasis cases in India
    Medhavi Sudarshan
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    PLoS Negl Trop Dis 8:e3366. 2014
    ..This study was designed to assess whether quantitative PCR (qPCR) can be used for detection of asymptomatic or early Leishmania donovani infection and as a predictor of progression to symptomatic disease...
  37. pmc Health & Demographic Surveillance System profile: the Muzaffarpur-TMRC Health and Demographic Surveillance System
    Paritosh Malaviya
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India, Institute of Tropical Medicine, Antwerp, Belgium and Barcelona Centre for International Health Research, Hospital Clínic Universitat de Barcelona, Barcelona, Spain Institute of Medical Sciences, Banaras Hindu University, Varanasi, India, Institute of Tropical Medicine, Antwerp, Belgium and Barcelona Centre for International Health Research, Hospital Clínic Universitat de Barcelona, Barcelona, Spain
    Int J Epidemiol 43:1450-7. 2014
    ..Queries related to research collaborations and data sharing can be sent to Dr Shyam Sundar at [drshyamsundar@hotmail.com].
  38. pmc A novel 12.6-kDa protein of Leishmania donovani for the diagnosis of Indian visceral leishmaniasis
    Dinesh Kumar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
    Vector Borne Zoonotic Dis 11:1359-64. 2011
    ..Unfortunately, up to 32% healthy individuals from endemic region test positive with this antigen. There is an urgent need to search for a more specific antigen with sensitivity similar to rK39...
  39. doi request reprint Oral miltefosine for Indian post-kala-azar dermal leishmaniasis: a randomised trial
    Shyam Sundar
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Trop Med Int Health 18:96-100. 2013
    ..Our objective was to evaluate oral miltefosine for its potential to provide effective as well as tolerable treatment for this disease...
  40. pmc Prevalence of sand flies and Leishmania donovani infection in a natural population of female Phlebotomus argentipes in Bihar State, India
    Puja Tiwary
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP, India
    Vector Borne Zoonotic Dis 12:467-72. 2012
    ..Sergentomyia species are the most common species of sand fly. Knowledge of the infection rate in female P. argentipes may help in predicting severity of disease and in vector elimination programs...
  41. doi request reprint Lay perceptions of kala-azar, mosquitoes and bed nets in Bihar, India
    Rabindra N Mishra
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Trop Med Int Health 15:36-41. 2010
    ....
  42. ncbi request reprint Evaluation of leishmanin skin test in Indian visceral leishmaniasis
    Kamlesh Gidwani
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 80:566-7. 2009
    ..Also, a lack of sensitivity of the LST was observed in cured VL patients. An LST antigen produced from L. donovani strains from India should be explored...
  43. pmc Evaluation of ex vivo human immune response against candidate antigens for a visceral leishmaniasis vaccine
    Rajiv Kumar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 82:808-13. 2010
    ..Further studies may help prioritize antigens for clinical development of a subunit vaccine against VL...
  44. pmc Evaluation of rk39 immunochromatographic test with urine for diagnosis of visceral leishmaniasis
    Jaya Chakravarty
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, U P, India
    Trans R Soc Trop Med Hyg 105:537-9. 2011
    ..With serum, sensitivity was 100% whereas the specificity was 100%, 92.4% and 95.55% for the respective control groups. In the present format, the immunochromatographic strips cannot be used for the diagnosis of VL using urine samples...
  45. ncbi request reprint Detection of leishmanial antigen in the urine of patients with visceral leishmaniasis by a latex agglutination test
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 73:269-71. 2005
    ..Positive and negative predictive values were 0.994 and 0.788, respectively. KAtex is a promising test, and in a simplified and improved format it could be applied meaningfully in the diagnosis of VL...
  46. pmc Study of parasite kinetics with antileishmanial drugs using real-time quantitative PCR in Indian visceral leishmaniasis
    Medhavi Sudarshan
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, UP, India
    J Antimicrob Chemother 66:1751-5. 2011
    ..This study describes parasite kinetics in the blood of visceral leishmaniasis patients treated with liposomal amphotericin B (L-AmB) or a preformed fat emulsion of amphotericin B (ApL) using real-time quantitative PCR (qPCR)...
  47. ncbi request reprint Amphotericin B treatment for Indian visceral leishmaniasis: conventional versus lipid formulations
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Banaras Hindu University, Institute of Medical Sciences, Varanasi, India
    Clin Infect Dis 38:377-83. 2004
    ....
  48. ncbi request reprint Visceral leishmaniasis - current therapeutic modalities
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences Banaras Hindu University, Varanasi, India
    Indian J Med Res 123:345-52. 2006
    ....
  49. ncbi request reprint Oral miltefosine for Indian visceral leishmaniasis
    Shyam Sundar
    Institutes of Medical Sciences, Banaras Hindu University, Varanasi, India
    N Engl J Med 347:1739-46. 2002
    ..We performed a clinical trial in India comparing miltefosine with the most effective standard treatment, amphotericin B...
  50. ncbi request reprint Endocrine changes in male HIV patients
    L P Meena
    Department of General Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP
    J Assoc Physicians India 59:365-6, 371. 2011
    ..To determine the frequency of adrenal, thyroid and gonadal dysfunction in HIV positive male patients and to evaluate the endocrine function at different level of CD4 cell counts...
  51. pmc PCR-RFLP based method for molecular differentiation of sand fly species Phlebotomus argentipes, Phlebotomus papatasi, and Sergentomyia babu found in India
    Puja Tiwary
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, U P, India
    J Med Entomol 49:1515-8. 2012
    ..Each of the restriction enzyme produced a species specific restriction patterns, which can easily be used to identify specific sand fly species. This technique can be used in the identification of sand fly species...
  52. ncbi request reprint Longitudinal seroepidemiologic study of visceral leishmaniasis in hyperendemic regions of Bihar, India
    Kamlesh Gidwani
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 80:345-6. 2009
    ..However, there was no significant difference in disease conversion among children (5-15 years of age) and adults (> 15 years of age). Seropositivity among asymptomatic persons is not a predictor for development of VL...
  53. ncbi request reprint High incidence of zidovudine induced anaemia in HIV infected patients in eastern India
    Dipti Agarwal
    ART Center, Department of Medicine, Institute of Medical Science, Banaras Hindu University, Varanasi, India
    Indian J Med Res 132:386-9. 2010
    ..This study was aimed at determining the prevalence of ZDV induced anaemia in HIV infected patients initiated on ZDV containing antiretroviral therapy regimen and also to find out the correlates, if any, for causing ZDV induced anaemia...
  54. pmc Noninvasive molecular diagnosis of human visceral leishmaniasis
    Manisha Vaish
    Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    J Clin Microbiol 49:2003-5. 2011
    ..This method is simple and well tolerated and has good potential for development, showing 83% sensitivity with 90.56% specificity in control groups...
  55. ncbi request reprint Compararative evaluation of rK9, rK26 and rK39 antigens in the serodiagnosis of Indian visceral leishmaniasis
    Tribhuban Mohan Mohapatra
    Department of Microbiology, Institute of Medical Sciences, BHU, Varanasi, India
    J Infect Dev Ctries 4:114-7. 2010
    ..chagasi) and crude soluble antigen (CSA) for the serodiagnosis of Indian visceral leishmaniasis (VL) patients by quantitative ELISA...
  56. pmc Evaluation of blood agar microtiter plates for culturing leishmania parasites to titrate parasite burden in spleen and peripheral blood of patients with visceral leishmaniasis
    Radheshyam Maurya
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    J Clin Microbiol 48:1932-4. 2010
    ..Plates could be easily monitored, and growth could be rapidly detected. Moreover, parasite number may be estimated using this technique...
  57. pmc Diagnosis of Indian visceral leishmaniasis by nucleic acid detection using PCR
    Pankaj Srivastava
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    PLoS ONE 6:e19304. 2011
    ..The present study was planned to design a Leishmania specific diagnostic assay and to evaluate its sensitivity and specificity on a sample size, which to the best of our knowledge is the largest ever screened in one study...
  58. pmc Interferon-gamma release assay (modified QuantiFERON) as a potential marker of infection for Leishmania donovani, a proof of concept study
    Kamlesh Gidwani
    Banaras Hindu University, Varanasi, India
    PLoS Negl Trop Dis 5:e1042. 2011
    ..We propose an interferon-gamma release assay (IFN-γRA) as a novel marker for latent L. donovani infection...
  59. doi request reprint Ambisome plus miltefosine for Indian patients with kala-azar
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    Trans R Soc Trop Med Hyg 105:115-7. 2011
    ..Clinical Trials.gov identification number: NCT00371995...
  60. pmc Identification and Characterization of a Novel, 37-Kilodalton Leishmania donovani antigen for diagnosis of Indian visceral leishmaniasis
    Subodh Kumar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    Clin Vaccine Immunol 18:772-5. 2011
    ..The antigen identified via a proteomics-based approach has a strong potential for further development as a diagnostic tool for VL...
  61. pmc Correlation between CD4 counts of HIV patients and enteric protozoan in different seasons - an experience of a tertiary care hospital in Varanasi (India)
    Lekha Tuli
    Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    BMC Gastroenterol 8:36. 2008
    ..This study is the first of its kind in this area where an attempt was made to correlate seasonal variation and intestinal protozoan infestations...
  62. pmc Immunoblot analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral leishmaniasis: its usefulness in prognosis
    Promod Kumar
    Department of Biochemistry, Faculty of Science, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    Clin Diagn Lab Immunol 9:1119-23. 2002
    ..Moreover, the persistence of reactivity with the 65- and 70-kDa bands in the sera of all groups shows its promise as a diagnostic and prognostic tool...
  63. ncbi request reprint Adenosine deaminase activity in sera of patients with visceral leishmaniasis in India
    Kiran Tripathi
    Department of Microbiology, Banaras Hindu University, Varanasi, India
    Clin Chim Acta 388:135-8. 2008
    ....
  64. ncbi request reprint Advances in the treatment of leishmaniasis
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Curr Opin Infect Dis 15:593-8. 2002
    ..In recent years, there has been a steady erosion in the efficacy of pentavalent antimony to cure visceral leishmaniasis in Bihar, India. In addition, several new antileishmanial formulations have become available...
  65. ncbi request reprint Paromomycin in the treatment of leishmaniasis
    Shyam Sundar
    Banaras Hindu University, Institute of Medical Sciences, Department of Medicine, Varanasi 221005, India
    Expert Opin Investig Drugs 17:787-94. 2008
    ..All these factors increase the cost of the treatment. The development of resistance to pentavalent antimonials in patients with visceral leishmaniasis in North Bihar, India, has added another dimension to the problem...
  66. pmc Evaluation of two novel rapid rKE16 antigen-based tests for diagnosis of visceral leishmaniasis in India
    Manisha Vaish
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
    J Clin Microbiol 50:3091-2. 2012
    ....
  67. ncbi request reprint Recent advances in the diagnosis and treatment of kala-azar
    Shyam Sundar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India
    Natl Med J India 25:85-9. 2012
    ..Early diagnosis, complete treatment of cases, integrated vector management, effective disease surveillance, and clinical and operational research should be the five key components of the strategy to achieve this goal...
  68. pmc Pharmacokinetics of oral sitamaquine taken with or without food and safety and efficacy for treatment of visceral leishmaniais: a randomized study in Bihar, India
    Shyam Sundar
    Kala Azar Medical Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 84:892-900. 2011
    ..8-94.4%) for sitamaquine and 95% (95% confidence interval = 75.1-99.9) for AmB. Sitamaquine can be taken regardless of food intake, was generally well tolerated, and showed potential efficacy in patients with visceral leishmaniasis...
  69. pmc Visceral leishmaniasis in Muzaffarpur district, Bihar, India from 1990 to 2008
    Paritosh Malaviya
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    PLoS ONE 6:e14751. 2011
    ..To understand the VL seasonality, annual and monthly variations of VL incidence and its relationship to meteorological variables, the numbers of VL cases reported in Muzaffarpur district, Bihar, India from 1990 to 2008 were studied...
  70. ncbi request reprint Ultrasonography for diagnosis of abdominal tuberculosis in HIV infected people
    Dipti Agarwal
    ART Center, Department of Medicine, Institute of Medical Science, Banaras Hindu University, Varanasi, India
    Indian J Med Res 132:77-80. 2010
    ..There is an increasing incidence of abdominal tuberculosis with the advent of HIV infection. This study was aimed at determining the pattern of presentation of abdominal tuberculosis on ultrasonography (USG) in HIV positive patients...
  71. doi request reprint The epidemiology of Leishmania donovani infection in high transmission foci in India
    Shri P Singh
    Banaras Hindu University, Varanasi, India
    Trop Med Int Health 15:12-20. 2010
    ..The prevalence of asymptomatic infections with Leishmania donovani and their role in transmission dynamics are not well understood. We report data from a sero-survey in Bihar...
  72. doi request reprint Household cost-of-illness of visceral leishmaniasis in Bihar, India
    Shyam Sundar
    Banaras Hindu University, Varanasi, India
    Trop Med Int Health 15:50-4. 2010
    ..To determine the cost of kala-azar (KA) to patients in Bihar, India...
  73. ncbi request reprint NADH-oxidase, NADPH-oxidase and myeloperoxidase activity of visceral leishmaniasis patients
    Promod Kumar
    Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, India
    J Med Microbiol 51:832-6. 2002
    ..These results suggest that low NADH-oxidase, NADPH-oxidase and myeloperoxidase activities may account for persistence of Leishmania parasites in visceral leishmaniasis...
  74. pmc rK39 antigen for the diagnosis of visceral leishmaniasis by using human saliva
    Manisha Vaish
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 86:598-600. 2012
    ..In serum, specificity was 97%, 88.5%, and 89% by ELISA and 100%, 94.7%, and 95.5% by ICT in nonendemic, endemic, and different diseases, respectively. Saliva is not suitable for diagnosis of VL because of low sensitivity...
  75. pmc Diagnosis of visceral leishmaniasis
    Pankaj Srivastava
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Trans R Soc Trop Med Hyg 105:1-6. 2011
    ..A simple, rapid, non invasive, accurate and cost effective marker of active VL, which can be used in field conditions, is necessary to improve diagnosis of VL...
  76. doi request reprint A clinical trial to evaluate the safety and immunogenicity of the LEISH-F1+MPL-SE vaccine for use in the prevention of visceral leishmaniasis
    Jaya Chakravarty
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Vaccine 29:3531-7. 2011
    ..This clinical trial shows that the LEISH-F1+MPL-SE vaccine is safe and immunogenic in healthy subjects with and without history of previous infection with Leishmania donovani...
  77. ncbi request reprint Knowledge, attitude, and practices related to Kala-azar in a rural area of Bihar state, India
    Shri P Singh
    Kala azar Medical Research Center, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Am J Trop Med Hyg 75:505-8. 2006
    ..Poor knowledge of the study subjects about the disease and breeding sites of the vector underscores the need for health educational campaigns if the elimination program is to succeed...
  78. ncbi request reprint Echocardiographic evaluation of cardiac status in Indian visceral leishmaniasis patients
    Rakesh Shrivastava
    Kala azar Medical Research Center, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
    Trans R Soc Trop Med Hyg 101:429-32. 2007
    ..Effusions were small, haemodynamically insignificant and resolved spontaneously. We conclude that pericardial effusion may occur in VL patients with heavy parasitaemia...
  79. pmc Antimony toxicity
    Shyam Sundar
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    Int J Environ Res Public Health 7:4267-77. 2010
    ..Quality control of each batch of drugs produced and regular monitoring for toxicity is required when antimonials are used therapeutically...
  80. pmc Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India
    Pankaj Srivastava
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, UP, India
    Infect Genet Evol 10:1145-50. 2010
    ..and L. donovani in this isolate. We consider that mixed infection may be present in the patients themselves, Leptomonas persisting in them because of the immuno-suppression associated with Kala-azar...
  81. pmc Genetic heterogeneity in clinical isolates of Leishmania donovani from India
    Pankaj Srivastava
    Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP, India 221005
    J Clin Microbiol 49:3687-90. 2011
    ..In contrast to earlier reports, our data suggest that significant genetic polymorphisms exist in L. donovani strains in Bihar, India. Our results confirm the presence of 2 zymodemes in India...
  82. ncbi request reprint Diagnosis and treatment of Indian visceral leishmaniasis
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005
    J Assoc Physicians India 51:195-201. 2003
  83. pmc No evidence for association between SLC11A1 and visceral leishmaniasis in India
    Sanjana Mehrotra
    Institute of Medical Sciences, Banaras Hindu University, Varanasi, OS 221 005, India
    BMC Med Genet 12:71. 2011
    ..Most studies undertaken to date were under-powered, and none has been replicated within a population. Association with tuberculosis has replicated variably across populations. Here we investigate SLC11A1 and VL in India...
  84. pmc Options for active case detection of visceral leishmaniasis in endemic districts of India, Nepal and Bangladesh, comparing yield, feasibility and costs
    Shri Prakash Singh
    Department of Community Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    PLoS Negl Trop Dis 5:e960. 2011
    ..This intervention study tests the yield, feasibility and cost of 4 different active case detection (ACD) strategies (camp, index case, incentive and blanket approach) in VL endemic districts of India, Nepal and Bangladesh...
  85. pmc Nano-structured nickel oxide based DNA biosensor for detection of visceral leishmaniasis (Kala-azar)
    Swati Mohan
    School of Materials Science and Technology, Institute of Technology, Banaras Hindu University, Varanasi, India
    Analyst 136:2845-51. 2011
    ..The observed results hold promise not only for diagnosis of Kala-azar patients but also hold enormous potential of the nano-NiO based probe for development of stable and sensitive biosensors...
  86. pmc Drug resistance in leishmaniasis
    Jaya Chakravarty
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    J Glob Infect Dis 2:167-76. 2010
    ..Regimens of simultaneous or sequential combinations should be seriously considered to limit the emergence of resistance...
  87. pmc Laboratory diagnosis of visceral leishmaniasis
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Banaras Hindu University, Institute of Medical Sciences, Varanasi 221 005, India
    Clin Diagn Lab Immunol 9:951-8. 2002
  88. pmc Diarrhea, CD4 counts and enteric infections in a hospital - based cohort of HIV-infected patients around Varanasi, India
    Suresh V S Attili
    Department of Medicine Institute of Medical Sciences, BHU, Varanasi 221005, India
    BMC Infect Dis 6:39. 2006
    ....
  89. ncbi request reprint Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda
    Philippe J Guerin
    Norwegian Institute of Public Health, Norway
    Lancet Infect Dis 2:494-501. 2002
    ..This article reviews the current situation and perspectives for diagnosis, treatment, and control of visceral leishmaniasis, and lists some priorities for research and development...
  90. ncbi request reprint Treatment options for visceral leishmaniasis: a systematic review of clinical studies done in India, 1980-2004
    Piero L Olliaro
    UNICEF UNDP World Bank WHO Special Programme on Research and Training in Tropical Diseases, WHO, Geneva, Switzerland
    Lancet Infect Dis 5:763-74. 2005
    ..India, Bangladesh, and Nepal agreed recently to undertake measures towards the elimination of visceral leishmaniasis. The lessons learnt in Bihar could help inform policy decisions both regionally and elsewhere...
  91. pmc Use of Leishmania donovani field isolates expressing the luciferase reporter gene in in vitro drug screening
    - Ashutosh
    Division of Biochemistry, Central Drug Research Institute, Chatter Manzil Palace, M G Road, Lucknow 226 001 UP, India
    Antimicrob Agents Chemother 49:3776-83. 2005
    ..The system is in routine use at the Central Drug Research Institute, Lucknow, India, for high-throughput screening of newly synthesized compounds...
  92. ncbi request reprint Drug uptake and modulation of drug resistance in Leishmania by an aquaglyceroporin
    Benjamin Gourbal
    Department of Biochemistry and Molecular Biology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:31010-7. 2004
    ..major, and the resulting cells became 10-fold more resistant to Sb(III). This is the first report of the uptake of a metalloid drug by an aquaglyceroporin in Leishmania, suggesting a strategy to reverse resistance in the field...
  93. ncbi request reprint Refractoriness to the treatment of sodium stibogluconate in Indian kala-azar field isolates persist in in vitro and in vivo experimental models
    Anuradha Dube
    Division of Parasitology, Central Drug Research Institute, Post Box No 173, Lucknow 226 001, India
    Parasitol Res 96:216-23. 2005
    ..Two isolates were also transfected with green fluorescent protein (GFP) for the development of in vitro assay for studying antileishmanial activities of new and reference drugs in macrophages by flow cytometry...
  94. ncbi request reprint Efficacy and tolerability of miltefosine for childhood visceral leishmaniasis in India
    Sujit K Bhattacharya
    Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, India
    Clin Infect Dis 38:217-21. 2004
    ..This trial indicates that miltefosine is as effective and well tolerated in Indian children with VL as in adults and that it can be recommended as the first choice for treatment of childhood VL in India...
  95. ncbi request reprint Challenges in the management of visceral leishmaniasis
    Shyam Sundar
    Indian Pediatr 42:523-6. 2005
  96. ncbi request reprint Variable degree of alternative complement pathway-mediated hemolysis in Indian visceral leishmaniasis induced by differential expression of 9-O-acetylated sialoglycans
    Anil Kumar Chava
    Immunobiology Division, Indian Institute of Chemical Biology, Jadavpur, Kolkata, India
    J Infect Dis 189:1257-64. 2004
    ..We assessed the capacity of this glycotope to influence hemolysis via activation of the alternative complement pathway in patients with VL, compared with that in healthy control subjects...
  97. ncbi request reprint A phase II dose-ranging study of sitamaquine for the treatment of visceral leishmaniasis in India
    Tara K Jha
    Kala azar Research Center, Muzaffarpur, India
    Am J Trop Med Hyg 73:1005-11. 2005
    ..Nephrotic syndrome (3% [3 of 120]) and glomerulonephritis (2% [2 of 120]) were also reported and require further investigation. Oral sitamaquine demonstrated efficacy in Indian VL and was well tolerated...
  98. ncbi request reprint Diagnosis of kala-azar--an important stride
    Shyam Sundar
    J Assoc Physicians India 51:753-5. 2003
  99. ncbi request reprint Phase 4 trial of miltefosine for the treatment of Indian visceral leishmaniasis
    Sujit Kumar Bhattacharya
    Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, India
    J Infect Dis 196:591-8. 2007
    ..Hospital-based studies have shown that oral miltefosine is a highly effective treatment for VL both in adults and in children...
  100. ncbi request reprint Possibility of membrane modification as a mechanism of antimony resistance in Leishmania donovani
    Hema Kothari
    Drug Target Discovery and Development Division, Central Drug Research Institute, Lucknow, India
    Parasitol Int 56:77-80. 2007
    ....
  101. pmc Inhibition of ABC transporters abolishes antimony resistance in Leishmania Infection
    Jayati Mookerjee Basu
    Department of Immunology, Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata 700032, WB, India
    Antimicrob Agents Chemother 52:1080-93. 2008
    ..Our results also indicate that lovastatin, which can inhibit both P-gp and MRP1, might be beneficial for reverting Sb resistance in leishmaniasis as well as drug resistance in other clinical situations, including cancer...