Poonam Salotra

Summary

Country: India

Publications

  1. pmc Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen
    P Salotra
    Molecular Biology Lab, Institute of Pathology ICMR, Safdarjung Hospital, New Delhi 110 029, India
    Clin Diagn Lab Immunol 9:370-3. 2002
  2. pmc Foxp3 and IL-10 expression correlates with parasite burden in lesional tissues of post kala azar dermal leishmaniasis (PKDL) patients
    Gajendra Kumar Katara
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    PLoS Negl Trop Dis 5:e1171. 2011
  3. pmc Evidence for involvement of Th17 type responses in post kala azar dermal leishmaniasis (PKDL)
    Gajendra Kumar Katara
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    PLoS Negl Trop Dis 6:e1703. 2012
  4. pmc Quantification of parasite load in clinical samples of leishmaniasis patients: IL-10 level correlates with parasite load in visceral leishmaniasis
    Sandeep Verma
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    PLoS ONE 5:e10107. 2010
  5. pmc Drug susceptibility in Leishmania isolates following miltefosine treatment in cases of visceral leishmaniasis and post kala-azar dermal leishmaniasis
    Vasundhra Bhandari
    National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India
    PLoS Negl Trop Dis 6:e1657. 2012
  6. pmc Analysis of localized immune responses reveals presence of Th17 and Treg cells in cutaneous leishmaniasis due to Leishmania tropica
    Gajendra Kumar Katara
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    BMC Immunol 14:52. 2013
  7. ncbi request reprint Heat-stress induced modulation of protein phosphorylation in virulent promastigotes of Leishmania donovani
    P Salotra
    Molecular Biology Laboratory, Institute of Pathology ICMR, New Delhi, India
    Int J Biochem Cell Biol 32:309-16. 2000
  8. ncbi request reprint Challenges in the diagnosis of post kala-azar dermal leishmaniasis
    Poonam Salotra
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    Indian J Med Res 123:295-310. 2006
  9. ncbi request reprint Upregulation of surface proteins in Leishmania donovani isolated from patients of post kala-azar dermal leishmaniasis
    Poonam Salotra
    Molecular Biology Laboratory, Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    Microbes Infect 8:637-44. 2006
  10. pmc Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis
    P Salotra
    Molecular Biology Lab, Institute of Pathology ICMR, Safdarjung Hospital Campus, Poat Box 4909, Safdarjung Hospital, New Delhi 110 029, India
    J Clin Microbiol 39:849-54. 2001

Collaborators

Detail Information

Publications49

  1. pmc Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen
    P Salotra
    Molecular Biology Lab, Institute of Pathology ICMR, Safdarjung Hospital, New Delhi 110 029, India
    Clin Diagn Lab Immunol 9:370-3. 2002
    ..5%) and specificity (93.7%) compared to the other two antigens used. The data demonstrate that ELISA with crude or recombinant antigen k39 provides a relatively simple and less-invasive test for the reliable diagnosis of PKDL...
  2. pmc Foxp3 and IL-10 expression correlates with parasite burden in lesional tissues of post kala azar dermal leishmaniasis (PKDL) patients
    Gajendra Kumar Katara
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    PLoS Negl Trop Dis 5:e1171. 2011
    ..The precise immunological cause of PKDL outcome remains obscure. However, overlapping counter regulatory responses with elevated IFN-γ and IL-10 are reported...
  3. pmc Evidence for involvement of Th17 type responses in post kala azar dermal leishmaniasis (PKDL)
    Gajendra Kumar Katara
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    PLoS Negl Trop Dis 6:e1703. 2012
    ..Parallel functioning of counter acting immune responses (Th1/Th2) reflects a complex immunological scenario, suggesting the involvement of additional regulatory molecules in the disease pathogenesis...
  4. pmc Quantification of parasite load in clinical samples of leishmaniasis patients: IL-10 level correlates with parasite load in visceral leishmaniasis
    Sandeep Verma
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    PLoS ONE 5:e10107. 2010
    ..The assay is applicable for diagnosis as well as prognosis of both VL and PKDL, providing a simple molecular tool to monitor the efficacy of antileishmanial drugs or vaccines...
  5. pmc Drug susceptibility in Leishmania isolates following miltefosine treatment in cases of visceral leishmaniasis and post kala-azar dermal leishmaniasis
    Vasundhra Bhandari
    National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India
    PLoS Negl Trop Dis 6:e1657. 2012
    ..Surveillance of MIL susceptibility in natural populations of Leishmania donovani is vital to preserve it and support the VL elimination program...
  6. pmc Analysis of localized immune responses reveals presence of Th17 and Treg cells in cutaneous leishmaniasis due to Leishmania tropica
    Gajendra Kumar Katara
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    BMC Immunol 14:52. 2013
    ..tropica infection. Expression of selected molecules was evaluated by real time PCR in dermal lesion tissues at pre- and post treatment stages. Plasma IL-17 level was estimated by sandwich ELISA...
  7. ncbi request reprint Heat-stress induced modulation of protein phosphorylation in virulent promastigotes of Leishmania donovani
    P Salotra
    Molecular Biology Laboratory, Institute of Pathology ICMR, New Delhi, India
    Int J Biochem Cell Biol 32:309-16. 2000
    ....
  8. ncbi request reprint Challenges in the diagnosis of post kala-azar dermal leishmaniasis
    Poonam Salotra
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    Indian J Med Res 123:295-310. 2006
    ..Diagnosis of PKDL presents a challenge due to low parasite burden in the lesions. In this article we have reviewed the recent advances in the development of immunological and molecular methods for diagnosis of PKDL...
  9. ncbi request reprint Upregulation of surface proteins in Leishmania donovani isolated from patients of post kala-azar dermal leishmaniasis
    Poonam Salotra
    Molecular Biology Laboratory, Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    Microbes Infect 8:637-44. 2006
    ..Such differentially expressed genes hold the key to understanding the parasite genetic factors that contribute to the persistence after clinical cure of VL...
  10. pmc Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis
    P Salotra
    Molecular Biology Lab, Institute of Pathology ICMR, Safdarjung Hospital Campus, Poat Box 4909, Safdarjung Hospital, New Delhi 110 029, India
    J Clin Microbiol 39:849-54. 2001
    ..Thus, this PCR assay represents a tool for the diagnosis of KA and PKDL in Indian patients in a noninvasive manner, with simultaneous species identification of parasites in clinical samples...
  11. ncbi request reprint A simple and sensitive test for field diagnosis of post kala-azar dermal leishmaniasis
    P Salotra
    Molecular Biology Laboratory, Institute of Pathology ICMR, Safdarjung Hospital Campus, Post Box 4909, New Delhi 110, 029, India
    Br J Dermatol 145:630-2. 2001
    ..Current methods for diagnosis of post kala-azar dermal leishmaniasis (PKDL) do not offer adequate sensitivity and specificity...
  12. ncbi request reprint Rapid & reliable diagnostic tests for visceral leishmaniasis
    Poonam Salotra
    Institute of Pathology ICMR Safdarjung Hospital Campus New Delhi 110029, India
    Indian J Med Res 122:464-7. 2005
  13. pmc Evaluation of localized and systemic immune responses in cutaneous leishmaniasis caused by Leishmania tropica: interleukin-8, monocyte chemotactic protein-1 and nitric oxide are major regulatory factors
    Rajesh Kumar
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi
    Immunology 130:193-201. 2010
    ..These data suggest that IL-8 is an effector immune-determinant in the progression of CL, whereas NO facilitates the parasite killing by macrophages via MCP-1-mediated stimulation...
  14. doi request reprint Biomarkers of antimony resistance: need for expression analysis of multiple genes to distinguish resistance phenotype in clinical isolates of Leishmania donovani
    Dhiraj Kumar
    National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India
    Parasitol Res 111:223-30. 2012
    ..As none of the genes exhibit an absolute correlation with phenotype, targeted expression analysis of a set of genes should be considered as biomarker for distinguishing the antimony-resistant and antimony-sensitive parasite...
  15. doi request reprint Comparative in vivo expression of amastigote up regulated Leishmania genes in three different forms of Leishmaniasis
    Paresh Sharma
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    Parasitol Int 59:262-4. 2010
    ..Assessment of in vivo mRNA level highlighted substantial differences in gene expression patterns, providing an indication of the genes involved in pathogenesis in the three different forms of Leishmaniasis...
  16. pmc In vitro susceptibility of field isolates of Leishmania donovani to Miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity
    Dhiraj Kumar
    Institute of Pathology, Safdarjung Hospital Campus, New Delhi, India
    Antimicrob Agents Chemother 53:835-8. 2009
    ..The results of gene expression analysis of LdMT and LdRos3 were suggestive of alternate mechanisms of Miltefosine susceptibility in the isolates...
  17. ncbi request reprint Overexpression of histone H2A modulates drug susceptibility in Leishmania parasites
    Ruchi Singh
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Int J Antimicrob Agents 36:50-7. 2010
    ..Moreover, H2A overexpression resulted in a significant decrease in susceptibility towards other antileishmanial drugs currently in use, i.e. amphotericin B and miltefosine, pointing to its role in drug resistance...
  18. ncbi request reprint Arbitrary-primed PCR for genomic fingerprinting and identification of differentially regulated genes in Indian isolates of Leishmania donovani
    G Sreenivas
    Institute of Pathology, Safdarjung Hospital, New Delhi 110029, India
    Exp Parasitol 106:110-8. 2004
    ..The study demonstrates the utility of random genome sampling methods in genomic fingerprinting and in identifying differentially transcribed sequences that could potentially contribute to parasite virulence...
  19. doi request reprint Presence of anti-Lepp12 antibody: a marker for diagnostic and prognostic evaluation of visceral leishmaniasis
    Dhiraj Kumar
    Molecular Biology Laboratory, Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Trans R Soc Trop Med Hyg 102:167-71. 2008
    ..rLepp12 protein provides a useful reagent for highly sensitive and specific diagnosis of KA. Additionally, rLepp12 appears to have potential as a prognostic marker for the infection...
  20. doi request reprint Increased parasite surface antigen-2 expression in clinical isolates of Leishmania donovani augments antimony resistance
    Vasundhra Bhandari
    National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India
    Biochem Biophys Res Commun 440:646-51. 2013
    ..The study established the role of PSA-2 gene in SAG resistance and its potential as a biomarker to distinguish resistant and sensitive clinical isolates of L. donovani...
  21. doi request reprint Correlation of parasitic load with interleukin-4 response in patients with cutaneous leishmaniasis due to Leishmania tropica
    Rajesh Kumar
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    FEMS Immunol Med Microbiol 57:239-46. 2009
    ..Upregulation of IL-4 was correlated with a higher parasite burden in early lesions of CL, which may be involved in the pathogenesis of CL by inhibiting a protective immune response...
  22. pmc Antimony-resistant clinical isolates of Leishmania donovani are susceptible to paromomycin and sitamaquine
    Arpita Kulshrestha
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Antimicrob Agents Chemother 55:2916-21. 2011
    ..Paromomycin, exhibiting higher efficacy against SAG-resistant parasites and having a distinct mechanism of action, appears to be a promising drug for combination therapy...
  23. doi request reprint Validation of a simple resazurin-based promastigote assay for the routine monitoring of miltefosine susceptibility in clinical isolates of Leishmania donovani
    Arpita Kulshrestha
    National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi 110029, India
    Parasitol Res 112:825-8. 2013
    ..Thus, our results support the utility of resazurin assay as a simplified biological tool for MIL susceptibility monitoring in clinical isolates from MIL-treated VL/PKDL patients...
  24. doi request reprint Immune response following miltefosine therapy in a patient with post-kala-azar dermal leishmaniasis
    Nasim Akhtar Ansari
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110 029, India
    Trans R Soc Trop Med Hyg 102:1160-2. 2008
    ..A significant increase in IFNgamma and CD40 levels seen after miltefosine therapy could enhance parasite clearance. The patient remained normal after 18 months of follow-up...
  25. ncbi request reprint Cutaneous leishmaniasis caused by Leishmania tropica in Bikaner, India: parasite identification and characterization using molecular and immunologic tools
    Rajesh Kumar
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    Am J Trop Med Hyg 76:896-901. 2007
    ..3% (25/28). Except for the kDNA PCR, all other assays were 100% specific. This study provides the first comprehensive molecular and immunologic studies of CL in India...
  26. pmc DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans
    Gannavaram Sreenivas
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    J Clin Microbiol 42:1739-41. 2004
    ....
  27. ncbi request reprint Visceral leishmaniasis, or kala azar (KA): high incidence of refractoriness to antimony is contributed by anthroponotic transmission via post-KA dermal leishmaniasis
    Ruchi Singh
    Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, Jiwaji University, Gwalior, India
    J Infect Dis 194:302-6. 2006
    ..05) than that for SAG-resistant KA isolates (15.7 microg/mL). SAG-resistant PKDL isolates may contribute to KA's increased refractoriness to SAG, via anthroponotic transmission of SAG-resistant strains...
  28. pmc Potential of direct agglutination test based on promastigote and amastigote antigens for serodiagnosis of post-kala-azar dermal leishmaniasis
    Ruchi Singh
    Institute of Pathology ICMR, New Delhi 110029, India
    Clin Diagn Lab Immunol 12:1191-4. 2005
    ..Thus, DAT provides a simple, reliable, and inexpensive test for PKDL diagnosis with potential applicability in field conditions...
  29. ncbi request reprint Genetic typing reveals monomorphism between antimony sensitive and resistant Leishmania donovani isolates from visceral leishmaniasis or post kala-azar dermal leishmaniasis cases in India
    B V Subba Raju
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Parasitol Res 111:1559-68. 2012
    ..Further, the Indian isolates were found closest to the Kenyan isolates of L. donovani on the basis of fragment analysis of microsatellite markers...
  30. doi request reprint Hypopigmented post-kala-azar dermal leishmaniasis
    V Ramesh
    Department of Dermatology and STD, Safdarjang Hospital and Vardhman Mahavir Medical College, All India Institute of Medical Sciences, New Delhi, India
    Int J Dermatol 47:414-6. 2008
  31. ncbi request reprint Short communication: post-kala-azar dermal leishmaniasis--an appraisal
    V Ramesh
    Department of Dermatology and Regional STD Centre, and Institute of Pathology ICMR, Safdarjang Hospital and V M Medical College, New Delhi, India
    Trop Med Int Health 12:848-51. 2007
    ..This short descriptive paper assesses diagnostics, clinical characteristics and therapy of both Indian and African post-kala-azar dermal leishmaniasis...
  32. pmc Nested PCR assay for detection of Leishmania donovani in slit aspirates from post-kala-azar dermal Leishmaniasis Lesions
    Gannavaram Sreenivas
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, India
    J Clin Microbiol 42:1777-8. 2004
    ..PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner...
  33. pmc In vitro susceptibility of Leishmania donovani to miltefosine in Indian visceral leishmaniasis
    Vijay Kumar Prajapati
    Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, UP, India Unit of Epidemiology and Control of Tropical Diseases, Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India Unit of Molecular Parasitology, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Department of Biomedical Sciences, Antwerp University, Antwerp, Belgium
    Am J Trop Med Hyg 89:750-4. 2013
    ..Our work highlights the need for careful monitoring of MIL susceptibility for implementation in national VL elimination programs...
  34. doi request reprint Diagnosis of visceral leishmaniasis: developments over the last decade
    Gurumurthy Srividya
    Molecular Biology Laboratory, National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Parasitol Res 110:1065-78. 2012
    ..This review summarizes the recent developments in VL diagnostics, drawing attention towards the need for standardization of the diagnostics across the affected regions...
  35. doi request reprint Cutaneous leishmaniasis in Nepal: Leishmania major as a cause
    Rajesh Kumar
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Trans R Soc Trop Med Hyg 102:202-3. 2008
    ..The patient was cured with sodium stibogluconate. This report suggests that the extent to which L. major causes CL in the Indian subcontinent needs to be monitored...
  36. doi request reprint A retrospective study of intravenous sodium stibogluconate alone and in combinations with allopurinol, rifampicin, and an immunomodulator in the treatment of Indian post-kala-azar dermal leishmaniasis
    V Ramesh
    Department of Dermatology and Regional STD Center and Institute of Pathology ICMR, Safdarjang Hospital and VM Medical College, New Delhi 110 029, India
    Indian J Dermatol Venereol Leprol 76:138-44. 2010
    ....
  37. ncbi request reprint Reliable diagnosis of post-kala-azar dermal leishmaniasis (PKDL) using slit aspirate specimen to avoid invasive sampling procedures
    Sandeep Verma
    National Institute of Pathology Indian Council of Medical Research, New Delhi, India
    Trop Med Int Health 18:268-75. 2013
    ..We evaluated the utility of minimally invasive slit aspirate specimen for serological and molecular diagnosis of PKDL. We compared the PKDL diagnosis using slit aspirate and skin biopsy specimens from the same patients...
  38. ncbi request reprint Interferon (IFN)-gamma , tumor necrosis factor-alpha , interleukin-6, and IFN-gamma receptor 1 are the major immunological determinants associated with post-kala azar dermal leishmaniasis
    Nasim A Ansari
    Institute of Pathology, Safdarjung Hospital Campus, New Delhi 110029, India
    J Infect Dis 194:958-65. 2006
    ..Therefore, unfavorable clinical evolution in PKDL may not be due to the absence of an intralesional Th1 response but rather may be due to the presence of counteracting cytokines along with the down-modulation of IFN-gamma R1...
  39. doi request reprint Application of loop-mediated isothermal amplification assay for the sensitive and rapid diagnosis of visceral leishmaniasis and post-kala-azar dermal leishmaniasis
    Sandeep Verma
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Diagn Microbiol Infect Dis 75:390-5. 2013
    ..L.) infantum, L. (L.) tropica, and L. (L.) major. This is the first comprehensive clinical study demonstrating the applicability of the LAMP assay for a rapid and reliable molecular diagnosis of VL and PKDL...
  40. ncbi request reprint Circulating nitric oxide and C-reactive protein levels in Indian kala azar patients: correlation with clinical outcome
    Nasim Akhtar Ansari
    Molecular Biology Lab, Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Clin Immunol 122:343-8. 2007
    ..Hence, data implies that the parasites survive in spite of the presence of effector molecules, and the excessive release of IFN-gamma and NO could be associated with the progression of the disease...
  41. ncbi request reprint Elevated levels of interferon-gamma, interleukin-10, and interleukin-6 during active disease in Indian kala azar
    Nasim Akhtar Ansari
    Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Clin Immunol 119:339-45. 2006
    ..The results depict that type1 response is not depressed during active KA and suggest the possibility that unresponsiveness to type1 stimuli may prevail...
  42. doi request reprint Comparative transcript expression analysis of miltefosine-sensitive and miltefosine-resistant Leishmania donovani
    Arpita Kulshrestha
    National Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi, 110029, India
    Parasitol Res 113:1171-84. 2014
    ..The study provided the comprehensive insight into the underlying mechanism of miltefosine resistance in L. donovani that may be useful to design strategies to increase lifespan of this important oral antileishmanial drug...
  43. pmc Elucidation of cellular mechanisms involved in experimental paromomycin resistance in Leishmania donovani
    Vasundhra Bhandari
    National Institute of Pathology, Indian Council of Medical Research, Safdarjung Hospital Campus, New Delhi, India
    Antimicrob Agents Chemother 58:2580-5. 2014
    ..The data implicated the roles of altered membrane fluidity, decreased drug accumulation, increased expression of ABC transporters, and greater tolerance of parasites to host defense mechanisms in conferring PMM resistance in Leishmania. ..
  44. ncbi request reprint An unusual presentation of post-kala-azar dermal leishmaniasis
    V Ramesh
    Department of Dermatology, Safdarjang Hospital, New Delhi 110 029, India
    Trop Doct 37:172-3. 2007
    ..An atypical presentation of post-kala-azar dermal leishmaniasis is reported to highlight the importance of suspecting dermatosis in patients from areas endemic for kala-azar...
  45. doi request reprint Deletion of mitochondrial associated ubiquitin fold modifier protein Ufm1 in Leishmania donovani results in loss of β-oxidation of fatty acids and blocks cell division in the amastigote stage
    Sreenivas Gannavaram
    Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA
    Mol Microbiol 86:187-98. 2012
    ..Therefore, these studies suggest the importance of Ufm1 in Leishmania pathogenesis with larger impact on other organisms and further provide an opportunity to test Ufm1(-/-) parasites as drug and vaccine targets...
  46. ncbi request reprint Nerve involvement in Indian post kala-azar dermal leishmaniasis
    Sujay Khandpur
    Acta Derm Venereol 84:245-6. 2004
  47. ncbi request reprint The application of gene expression microarray technology to kinetoplastid research
    Robert C Duncan
    Division of Emerging and Transfusion Transmitted Diseases, Office of Blood Research and Review, CBER, FDA, Bethesda, MD, USA
    Curr Mol Med 4:611-21. 2004
    ....
  48. doi request reprint Indian mucosal leishmaniasis due to Leishmania donovani infection
    Gomathy Sethuraman
    N Engl J Med 358:313-5. 2008
  49. pmc A novel semiquantitative fluorescence-based multiplex polymerase chain reaction assay for rapid simultaneous detection of bacterial and parasitic pathogens from blood
    Angamuthu Selvapandiyan
    Division of Emerging and Transfusion Transmitted Disease, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    J Mol Diagn 7:268-75. 2005
    ..The time necessary for performing this assay including sample preparation was less than 1.5 hours, making this a potentially useful method for rapidly diagnosing and monitoring the efficacy of drugs or vaccines in infected individuals...