Williams D, Slayden R, Amin A, Martinez A, Pittman T, Mira A, et al
. Implications of high level pseudogene transcription in Mycobacterium leprae. BMC Genomics. 2009;10:397 pubmed publisher
..Preliminary evidence suggests that some pseudogenes are expressed. Therefore, defining pseudogene transcriptional and translational potentials of this genome should increase our understanding of their impact on M. leprae physiology...
Godbole A, Ahmed W, Bhat R, Bradley E, Ekins S, Nagaraja V. Targeting Mycobacterium tuberculosis topoisomerase I by small-molecule inhibitors. Antimicrob Agents Chemother. 2015;59:1549-57 pubmed publisher
..smegmatis topoisomerase I, as leads for the development of improved molecules to combat mycobacterial infections. Moreover, targeting metal coordination in topoisomerases might be a general strategy to develop new lead molecules. ..
Ahmed W, Menon S, Karthik P, Nagaraja V. Autoregulation of topoisomerase I expression by supercoiling sensitive transcription. Nucleic Acids Res. 2016;44:1541-52 pubmed publisher
..We propose that negative supercoiling induced DNA twisting/writhing align the -35 and -10 elements to facilitate the optimal transcription of topoI. ..
Ghosh S, Padmanabhan B, Godbole A, Tare P, Ahmed W, Vasu K, et al
. Transcriptional regulation of topology modulators and transcription regulators of Mycobacterium tuberculosis. Biochem Biophys Res Commun. 2016;475:257-63 pubmed publisher
..These data provide the first glimpse into the specific adaptive changes in the expression of genes involved in transcription and DNA topology modulation in Mtb. ..
Ghosh S, Mallick B, Nagaraja V. Direct regulation of topoisomerase activity by a nucleoid-associated protein. Nucleic Acids Res. 2014;42:11156-65 pubmed publisher
..The species-specific physical and functional cooperation between MtHU and TopoI may be the key to achieve the DNA relaxation levels needed to maintain the optimal superhelical density of mycobacterial genomes. ..
Ahmed W, Menon S, Karthik P, Nagaraja V. Reduction in DNA topoisomerase I level affects growth, phenotype and nucleoid architecture of Mycobacterium smegmatis. Microbiology. 2015;161:341-53 pubmed publisher
..A decrease in TopoI level led to altered RNAP occupancy and impaired transcription elongation, causing severe downstream effects. ..
Ekins S, Godbole A, KÃ©ri G, Orfi L, Pato J, Bhat R, et al
. Machine learning and docking models for Mycobacterium tuberculosis topoisomerase I. Tuberculosis (Edinb). 2017;103:52-60 pubmed publisher
..We demonstrate the experimental inhibition of Mttopo I by small molecule inhibitors and show that the enzyme can be readily targeted for lead molecule development. ..
Suarez M, Valencia J, Cadena C, Maiti R, Datta C, Puerto G, et al
. Diarylethenes Display In Vitro Anti-TB Activity and Are Efficient Hits Targeting the Mycobacterium tuberculosis HU Protein. Molecules. 2017;22: pubmed publisher
..The most selective hit (SI = 81), demonstrated inhibition of M. tuberculosis HU protein involved in maintaining bacterial genome architecture. ..
Nagamalleswari E, Nagaraja V. Draft Genome Sequence of Klebsiella pneumoniae OK8, a Multidrug-Resistant Mouse and Human Pathogen. Genome Announc. 2017;5: pubmed publisher
..We report here the draft genome sequence of Klebsiella pneumoniae OK8, a multidrug-resistant strain which was isolated in 1976 from a human and is known to be a mouse pathogen. ..