R Tuteja

..This review presents an update of the studies carried out in malaria using DNA vaccine approach, the challenges, and the future prospects...

..These detailed studies may help to identify a parasite-specific enzyme, which could be a potential drug target to treat malaria. The various steps at which this probable drug can act are also discussed...

..Further characterization of these helicases will help in understanding physiological significance of these helicases in filarial parasites, which in future can be utilized for chemotherapy of parasitic infection...

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..These studies demonstrate the characterization of a functional subunit of SPC from P. falciparum and should make an important contribution in our better understanding of the complex process of protein translocation in the parasite...

..In this review we present an outline of the method and updated information on the applications of SAGE technology to various cancers...

..In this review, we present an outline of the method, various bioinformatics methods for data analysis and general applications of this important technology...

..One feasible target might be helicases, which are important unwinding enzymes and required for almost all the nucleic acid metabolism in the malaria parasite...

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..Type I signal peptidases have been found in bacteria, archaea, fungi, plants, and animals. In this review, I present an overview of bacterial type I signal peptidases and describe some of their properties in detail...

..These studies demonstrate that these factors are structurally and functionally conserved. These studies will contribute to understand the important process and components of translation complex in the malaria parasite...

..falciparum. The detailed studies of these helicases will help in identifying a specific enzyme, which could be used as potential target to control the replication and transmission of the malaria parasite...

..This article will focus on members of the DExD/H-box proteins involved specially in splicing in the malaria parasite Plasmodium falciparum...

..These include the technical challenges of improving delivery and/or potency so that low doses of DNA can achieve the efficacy of conventional vaccines...

..The mechanism underlying the regulation of all the diverse functions of Ku is still obscure...

..We further show that the immune responses generated by plasmid pcDNA-ORF2 can be boosted with virus-like particles composed of the ORF2 protein expressed through a baculovirus expression system...

..These studies show that these TFs are slowly emerging to have defined roles in the regulation of gene expression in the parasite...

..This review describes the biochemistry of various repair processes and summarizes the clinical features and molecular mechanisms underlying these disorders...

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..In the present study we have reported the detailed characterization of PfU52 from P. falciparum and these results advance the knowledge regarding the function of UAP56 in general...

..These studies indicate that PfH45 is an indispensable enzyme that is essential for growth, and probably survival, of P. falciparum...

..In plants it is developmentally, cell-cycle, and light regulated. The regulation of all these functions of a single protein seems to be a challenging puzzle...

..These observations suggest that PDH65 may be involved both in rDNA transcription and in the early stages of pre-rRNA processing...

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..This inhibition could be due to binding of these drugs to DNA, thereby impeding the movement of the helicase for unwinding action which may be their most important pharmacological function against cancer cells...

..Our results revealed that expression of helicase genes is profoundly regulated under various stress conditions. The helicases identified in this study lay a foundation for the in depth characterization of each helicase type...

..This article describes the insight underlying the DNA damage and repair pathways in plants. The comet assay to measure the DNA damage and the role of DNA repair helicases such as XPD and XPB are also covered...

..The properties of most of the known DNA helicases from prokaryotic and eukaryotic systems, including viruses and bacteriophages, are summarized in tables...

..These studies suggest that besides the conserved helicase domain the highly variable N-terminal region also contributes in the activity of the protein...

..Motif 1 (EVF) is conserved in Ps.SEO1, Vicia faba (Vf.For1) and Medicago truncatula (Mt.SEO3); motif 2 (KKED) is well conserved across all forisomes proteins and motif 3 (IGYIGNP) is conserved in Ps.SEO1 and Vf.For1...

..5 to 5.0 microM respectively. These compounds distinctively inhibit the helicase activity probably by forming complex with DNA and obstructing enzyme movement...

..In this manuscript we provide an overview of UvrD family of helicases and bioinformatics analysis of UvrD from P. falciparum...

..The genes containing helicase motifs may have evolved from a common ancestor. In this review we cover the conserved motifs, structural information, mechanism of DNA unwinding and translocation, and functional aspects of DNA helicases...

..In the present study, we report some of the important activities of the encoded protein...

..Its bipolar helicase activities may also be involved in distinct cellular processes in stressed conditions...

..The cross-talk between plant MAP kinases is also discussed to bring out the complexity within this three-component module...

..We have also shown the various steps of translocation in a hypothetical model...

..Thus, it would appear that the kinetics of Ag recognition dominate over equilibrium binding criteria both in epitope-driven repertoire selection and Ab maturation in a humoral response...

..This study should make important contribution to our better understanding of DNA transaction in plants, mechanism of DNA unwinding, and the mechanism by which these ligands can disturb genome integrity...

..Isolation of this new DNA helicase should make an important contribution to our better understanding of DNA transaction in plants...

..This discovery should make an important contribution to a better understanding of DNA replication in plants...

..These compounds also inhibited parasite growth in culture. This is the first study to show inhibition of growth of the parasite by the dsRNA of a helicase, and most probably this is due to interference with cognate mRNA expression...

..Using this approach, we show that helicases are indispensable enzymes, which are required for growth and most probably survival of the malaria parasite...