C E Chitnis

Summary

Affiliation: International Centre for Genetic Engineering and Biotechnology
Country: India

Publications

  1. ncbi request reprint Molecular analysis of the cytoadherence phenotype of a Plasmodium falciparum field isolate that binds intercellular adhesion molecule-1
    Rana Chattopadhyay
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Biochem Parasitol 133:255-65. 2004
  2. pmc Distinct external signals trigger sequential release of apical organelles during erythrocyte invasion by malaria parasites
    Shailja Singh
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
    PLoS Pathog 6:e1000746. 2010
  3. pmc Association of severe malaria outcomes with platelet-mediated clumping and adhesion to a novel host receptor
    Alfredo Mayor
    Barcelona Centre for International Health Research CRESIB, Hospital Clínic Institut d Investigacions Biomèdiques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, Barcelona, Spain
    PLoS ONE 6:e19422. 2011
  4. pmc Structural insights into chondroitin sulphate A binding Duffy-binding-like domains from Plasmodium falciparum: implications for intervention strategies against placental malaria
    Jasmita Gill
    Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology ICGEB, New Delhi, India
    Malar J 8:67. 2009
  5. pmc Genome-wide SNP genotyping highlights the role of natural selection in Plasmodium falciparum population divergence
    Daniel E Neafsey
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    Genome Biol 9:R171. 2008
  6. ncbi request reprint Molecular insights into receptors used by malaria parasites for erythrocyte invasion
    C E Chitnis
    Malaria Research Group, International Center for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi, India
    Curr Opin Hematol 8:85-91. 2001
  7. ncbi request reprint Targeting the Plasmodium vivax Duffy-binding protein
    Chetan E Chitnis
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India
    Trends Parasitol 24:29-34. 2008
  8. pmc Immunogenicity of Duffy binding-like domains that bind chondroitin sulfate A and protection against pregnancy-associated malaria
    Nivedita Bir
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Infect Immun 74:5955-63. 2006
  9. ncbi request reprint Bacterially expressed and refolded receptor binding domain of Plasmodium falciparum EBA-175 elicits invasion inhibitory antibodies
    Kailash C Pandey
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, P O Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Biochem Parasitol 123:23-33. 2002
  10. ncbi request reprint Structural basis for Duffy recognition by the malaria parasite Duffy-binding-like domain
    Saurabh Kumar Singh
    Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Nature 439:741-4. 2006

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Molecular analysis of the cytoadherence phenotype of a Plasmodium falciparum field isolate that binds intercellular adhesion molecule-1
    Rana Chattopadhyay
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Biochem Parasitol 133:255-65. 2004
    ..falciparum isolates use a structurally conserved domain to bind ICAM-1. It thus appears that functional constraints may place limits on the extent of sequence diversity in receptor-binding domains of PfEMP-1...
  2. pmc Distinct external signals trigger sequential release of apical organelles during erythrocyte invasion by malaria parasites
    Shailja Singh
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
    PLoS Pathog 6:e1000746. 2010
    ..Signaling pathway components involved in apical organelle discharge may serve as novel targets for drug development since inhibition of microneme and rhoptry secretion can block invasion and limit blood-stage parasite growth...
  3. pmc Association of severe malaria outcomes with platelet-mediated clumping and adhesion to a novel host receptor
    Alfredo Mayor
    Barcelona Centre for International Health Research CRESIB, Hospital Clínic Institut d Investigacions Biomèdiques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, Barcelona, Spain
    PLoS ONE 6:e19422. 2011
    ..Identification of P. falciparum cytoadherence phenotypes that are associated with severe malaria may lead to the development of novel strategies against life-threatening malaria...
  4. pmc Structural insights into chondroitin sulphate A binding Duffy-binding-like domains from Plasmodium falciparum: implications for intervention strategies against placental malaria
    Jasmita Gill
    Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology ICGEB, New Delhi, India
    Malar J 8:67. 2009
    ..Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) encoded by the var2CSA gene is believed to be the main parasite ligand for CSA-mediated placental binding...
  5. pmc Genome-wide SNP genotyping highlights the role of natural selection in Plasmodium falciparum population divergence
    Daniel E Neafsey
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    Genome Biol 9:R171. 2008
    ..To that end, we have developed a high throughput single nucleotide polymorphism (SNP) genotyping platform for P. falciparum...
  6. ncbi request reprint Molecular insights into receptors used by malaria parasites for erythrocyte invasion
    C E Chitnis
    Malaria Research Group, International Center for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi, India
    Curr Opin Hematol 8:85-91. 2001
    ..Clear understanding of these interactions is important for the rational design of vaccines that attempt to inhibit invasion and prevent malaria...
  7. ncbi request reprint Targeting the Plasmodium vivax Duffy-binding protein
    Chetan E Chitnis
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India
    Trends Parasitol 24:29-34. 2008
    ..Here, we review data on the interaction of PvRII with DARC and explore the potential of targeting this crucial receptor-ligand interaction to develop new intervention strategies against P. vivax...
  8. pmc Immunogenicity of Duffy binding-like domains that bind chondroitin sulfate A and protection against pregnancy-associated malaria
    Nivedita Bir
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Infect Immun 74:5955-63. 2006
    ..Such cross-reactive epitopes within CSA-binding DBL domains can form the basis for a vaccine that provides protection against PAM...
  9. ncbi request reprint Bacterially expressed and refolded receptor binding domain of Plasmodium falciparum EBA-175 elicits invasion inhibitory antibodies
    Kailash C Pandey
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, P O Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Biochem Parasitol 123:23-33. 2002
    ..falciparum field isolate that invades erythrocytes using multiple pathways. These observations support the development of recombinant PfF2 as a vaccine candidate for P. falciparum malaria...
  10. ncbi request reprint Structural basis for Duffy recognition by the malaria parasite Duffy-binding-like domain
    Saurabh Kumar Singh
    Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Nature 439:741-4. 2006
    ..vivax. Our comparative structural analysis of Pkalpha-DBL and P. falciparum EBA-175 provides a framework for the understanding of malaria parasite DBLs, and may affect the development of new prophylactic and therapeutic strategies...
  11. ncbi request reprint Evaluation of immune responses elicited in mice against a recombinant malaria vaccine based on Plasmodium vivax Duffy binding protein
    Syed Shams Yazdani
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, P O Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Vaccine 22:3727-37. 2004
    ....
  12. pmc Definition of structural elements in Plasmodium vivax and P. knowlesi Duffy-binding domains necessary for erythrocyte invasion
    Saurabh K Singh
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
    Biochem J 374:193-8. 2003
    ..These studies pave the way for understanding the architecture of Duffy-binding domains and their interactions with host receptors...
  13. ncbi request reprint Antibodies raised against receptor-binding domain of Plasmodium knowlesi Duffy binding protein inhibit erythrocyte invasion
    Agam P Singh
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi, India
    Mol Biochem Parasitol 121:21-31. 2002
    ..knowlesi invasion of both human and rhesus erythrocytes. These data provide support for the development of recombinant vaccines based on the homologous binding domains of P. vivax Duffy binding protein and P. falciparum EBA-175...
  14. ncbi request reprint Immunogenicity of Plasmodium vivax combination subunit vaccine formulated with human compatible adjuvants in mice
    Yengkhom Sangeeta Devi
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, P O Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Vaccine 25:5166-74. 2007
    ..The results of the present study provide a rationale for a combination vaccine against P. vivax malaria based on PvMSP1(19) and PvRII...
  15. ncbi request reprint Receptor-binding residues lie in central regions of Duffy-binding-like domains involved in red cell invasion and cytoadherence by malaria parasites
    Alfredo Mayor
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, PO Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Blood 105:2557-63. 2005
    ..Determination of functional regions within DBL domains is the first step toward understanding the structure-function bases for their interaction with diverse host receptors...
  16. ncbi request reprint Mapping binding residues in the Plasmodium vivax domain that binds Duffy antigen during red cell invasion
    Dhiraj Hans
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Microbiol 55:1423-34. 2005
    ..Genetic diversity studies have shown that six of the seven binding residues identified in PvRII are conserved in P. vivax field isolates, which provides support for their role in interaction with DARC...
  17. ncbi request reprint Sequence polymorphisms in the receptor-binding domain of Plasmodium falciparum EBA-175: implications for malaria vaccine development
    Anitha Mamillapalli
    Malaria Research Centre, New Delhi, India
    Mol Biochem Parasitol 146:120-3. 2006
  18. pmc Plasmodium falciparum infection elicits both variant-specific and cross-reactive antibodies against variant surface antigens
    Rana Chattopadhyay
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, New Delhi, India
    Infect Immun 71:597-604. 2003
    ..Analysis of PfEMP-1 sequences suggests a molecular basis for the observed cross-reactivity...
  19. ncbi request reprint Host cell invasion by malaria parasites
    C E Chitnis
    Malaria Group, International Centre for Genetic Engineering and Biology, 110017, New Delhi, India
    Parasitol Today 16:411-5. 2000
    ....
  20. ncbi request reprint Structural analogs of sialic acid interfere with the binding of erythrocyte binding antigen-175 to glycophorin A, an interaction crucial for erythrocyte invasion by Plasmodium falciparum
    Ritica Bharara
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, P O Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Biochem Parasitol 138:123-9. 2004
    ..Such compounds may form part of inhibitor cocktails, which aim to block invasion of erythrocytes by P. falciparum...
  21. ncbi request reprint A high cell density fermentation strategy to produce recombinant malarial antigen in E. coli
    Syed Shams Yazdani
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Biotechnol Lett 26:1891-5. 2004
    ..After refolding, PvRII was greater than 98% pure, homogeneous and functionally active in that it specifically bound Duffy positive human red cells...
  22. ncbi request reprint Improvement in yield and purity of a recombinant malaria vaccine candidate based on the receptor-binding domain of Plasmodium vivax Duffy binding protein by codon optimization
    Syed Shams Yazdani
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi, India
    Biotechnol Lett 28:1109-14. 2006
    ..Expression level of recombinant PvRII was 10% of the total cellular proteins. Truncated PvRII products, seen when the native PvRII gene was expressed, were absent in case of synthetic gene...
  23. ncbi request reprint Targeted deletion of Plasmodium knowlesi Duffy binding protein confirms its role in junction formation during invasion
    Agam P Singh
    Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
    Mol Microbiol 55:1925-34. 2005
    ..These studies provide support for development of intervention strategies that target EBPs to inhibit junction formation and block erythrocyte invasion by malaria parasites...
  24. ncbi request reprint Immune responses to asexual blood-stages of malaria parasites
    S S Yazdani
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, New Delhi, India
    Curr Mol Med 6:187-203. 2006
    ..falciparum endemic areas and review their role in providing protection against malaria. In addition, we review the dual potential of cellular and innate immune responses to control parasite multiplication and promote pathology...
  25. ncbi request reprint A Plasmodium yoelii yoelii erythrocyte binding protein that uses Duffy binding-like domain for invasion: a rodent model for studying erythrocyte invasion
    C Durga Prasad
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India
    Mol Biochem Parasitol 128:101-5. 2003
  26. ncbi request reprint Immunogenicity of a recombinant malaria vaccine based on receptor binding domain of Plasmodium falciparum EBA-175
    Priyabrata Pattnaik
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, P O Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
    Vaccine 25:806-13. 2007
    ....
  27. doi request reprint Localization of apical sushi protein in Plasmodium falciparum merozoites
    Anand Srivastava
    Malaria Group, International Centre for Genetic Engineering and Biotechnology ICGEB, Aruna Asaf Ali Marg, New Delhi 110067, India
    Mol Biochem Parasitol 174:66-9. 2010
    ..falciparum merozoites. In the present study, we have used indirect immunofluorescence assays and immunoelectron microscopy to demonstrate that PfASP is localized in the neck of rhoptries and not in micronemes as previously described...
  28. pmc Plasmodium falciparum uses gC1qR/HABP1/p32 as a receptor to bind to vascular endothelium and for platelet-mediated clumping
    Anup Kumar Biswas
    Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
    PLoS Pathog 3:1271-80. 2007
    ..Given the association of adhesion to vascular endothelium and platelet-mediated clumping with severe disease, adhesion to gC1qR/HABP1/p32 by P. falciparum IRBCs may play an important role in malaria pathogenesis...
  29. ncbi request reprint Immunogenicity and protective efficacy of recombinant vaccine based on the receptor-binding domain of the Plasmodium vivax Duffy binding protein in Aotus monkeys
    Myriam Arevalo-Herrera
    Institute of Immunology, Universidad del Valle, Cali, Colombia
    Am J Trop Med Hyg 73:25-31. 2005
    ..Given the immunogenicity and partial protection observed, further studies are needed to optimize the PvRII vaccine formulation with adjuvants suitable for human use...
  30. pmc Naturally acquired Duffy-binding protein-specific binding inhibitory antibodies confer protection from blood-stage Plasmodium vivax infection
    Christopher L King
    Case Western Reserve University Center for Global Health and Diseases, Cleveland, OH 44106
    Proc Natl Acad Sci U S A 105:8363-8. 2008
    ..vivax strains characterized by six diverse PvDBPII haplotypes. These observations demonstrate a functional correlate of protective immunity in vivo and provide support for developing a vaccine against P. vivax malaria based on PvDBPII...
  31. pmc Impact of intermittent preventive treatment with sulfadoxine-pyrimethamine on antibody responses to erythrocytic-stage Plasmodium falciparum antigens in infants in Mozambique
    Diana Quelhas
    Centro de Investigação em Saúde da Manhiça CISM, Manhiça, Mozambique
    Clin Vaccine Immunol 15:1282-91. 2008
    ..IPTi with SP given through the EPI reduces the frequency of malarial illness while allowing the development of naturally acquired antibody responses to P. falciparum antigens...
  32. pmc Epitope-specific humoral immunity to Plasmodium vivax Duffy binding protein
    Jia Xainli
    Center for Global Health and Diseases, Case Western Reserve University, Cleveland, Ohio 44106 4983, USA
    Infect Immun 71:2508-15. 2003
    ..In conclusion, these results demonstrate age-dependent and variant-specific antibody responses to DBPII and implicate this molecule in partial acquired immunity to P. vivax in populations in endemic areas...
  33. ncbi request reprint Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax
    Mayara B Barbedo
    Departamento de Analises Clinicas e Toxicologicas, Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo, Súo Paulo, Brasil
    Mem Inst Oswaldo Cruz 102:335-9. 2007
    ..The reason for that is presently unknown but it may contribute to the high risk of re-infection in individuals living in the endemic areas...
  34. ncbi request reprint Detection of a Plasmodium vivax erythrocyte binding protein by flow cytometry
    Tuan M Tran
    Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, Georgia 30329, USA
    Cytometry A 63:59-66. 2005
    ..vivax Duffy binding protein region II (PvDBP-RII) recombinant protein as a model. This protein binds to all erythrocytes that express the Duffy receptor (Fy) and discriminates binding between normocytes and reticulocytes...
  35. ncbi request reprint Sulphated tyrosines mediate association of chemokines and Plasmodium vivax Duffy binding protein with the Duffy antigen/receptor for chemokines (DARC)
    Hyeryun Choe
    Perlmutter Laboratory, Children s Hospital, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 55:1413-22. 2005
    ..These data are consistent with a role for tyrosine sulphation in the association of many or most chemokines with their receptors, and identify a key molecular determinant of erythrocyte invasion by P. vivax...
  36. ncbi request reprint Antibody response to Plasmodium vivax antigens in Fy-negative individuals from the Colombian Pacific coast
    Socrates Herrera
    Malaria Vaccine and Drug Development Center, Cali, Colombia
    Am J Trop Med Hyg 73:44-9. 2005
    ..Thus, Fy-negative individuals from malaria-endemic areas can be used to study the immune response to the P. vivax liver phase without interference of the erythrocytic cycle...
  37. ncbi request reprint Comparison of IgG reactivities to Plasmodium vivax merozoite invasion antigens in a Brazilian Amazon population
    Tuan M Tran
    Emory Vaccine Center and Yerkes National Primate Research Center, Emory University, Atlanta, Georgia 30329, USA
    Am J Trop Med Hyg 73:244-55. 2005
    ....
  38. ncbi request reprint Pfcrt haplotypes and in-vivo chloroquine response in Sundergarh district, Orissa, India
    Sudhanshu S Pati
    Department of Biochemistry and Molecular Biology, Ispat General Hospital, Rourkela 769005, Orissa, India
    Trans R Soc Trop Med Hyg 101:650-4. 2007
    ..The CQ-sensitive haplotypes were mostly CVMNK in 10 samples; CVIEK in 2 samples. Both Southeast Asian and South American haplotypes were present, with the latter predominating...
  39. ncbi request reprint Divergent binding sites on intercellular adhesion molecule-1 (ICAM-1) for variant Plasmodium falciparum isolates
    Man Tsuey Tse
    Liverpool School of Tropical Medicine, Pembroke Place, UK
    Mol Microbiol 51:1039-49. 2004
    ..The definition of common binding determinants and the elucidation of links between ICAM-1 binding phenotype and disease will provide new leads in the design of therapeutic interventions...
  40. ncbi request reprint Expression and characterization of human malaria parasite Plasmodium falciparum origin recognition complex subunit 1
    Parul Mehra
    Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 67, India
    Biochem Biophys Res Commun 337:955-66. 2005
    ..These observations strongly suggest that PfORC1 might be involved in DNA replication initiation during the blood stage of the parasitic life cycle...