Research Topics
| R GuptaSummaryCountry: India Publications
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Detail Information
Publications
Bacterial alkaline proteases: molecular approaches and industrial applicationsR Gupta
Department of Microbiology, University of Delhi South Campus, Benito Juarez Marg, New Delhi 110 021, India
Appl Microbiol Biotechnol 59:15-32. 2002....
An overview on fermentation, downstream processing and properties of microbial alkaline proteasesR Gupta
Department of Microbiology, University of Delhi South Campus, Benito Juarez Marg, New Delhi 110 021, India
Appl Microbiol Biotechnol 60:381-95. 2002..Several industrially important proteases have been subjected to crystallization to extensively study their molecular homology and three-dimensional structures...
Bacterial lipases: an overview of production, purification and biochemical propertiesR Gupta
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, 110 021 New Delhi, India
Appl Microbiol Biotechnol 64:763-81. 2004..The latest trend in lipase research is the development of novel and improved lipases through molecular approaches such as directed evolution and exploring natural communities by the metagenomic approach...
Cullen's sign in amoebic liver abscessA Misra
Gastroenterology Unit, Department of Medicine, MLN Medical College, Allahabad, India
Postgrad Med J 78:427-8. 2002..Ultrasound of her abdomen showed a large liver abscess with subhepatic collection. She had Cullen's sign. The liver abscess was managed by percutaneous catheter drainage...
Statistical medium optimization and production of a hyperthermostable lipase from Burkholderia cepacia in a bioreactorP Rathi
Department of Microbiology, University of Delhi South Campus, New Delhi, India
J Appl Microbiol 93:930-6. 2002..SIGNIFICANCE AND IMPACT OF THE STUDY: Statistical optimization allows quick optimization of a large number of variables. It also provides a deep insight into the regulatory role of various parameters involved in enzyme production...
Biocatalytic potential of Fusarium globulosum lipase in selective acetylation/deacetylation reactions and in ester synthesisR Gulati
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110 021, India
J Appl Microbiol 90:609-13. 2001..These compounds find widespread uses as surfactants, bioactive analogues, antioxidants and flavour components in the food, detergent, pharmaceutical and cosmetic industries...
Gene cloning, expression, and characterization of the Sac7 proteins from the hyperthermophile Sulfolobus acidocaldariusJ G McAfee
Department of Medical Biochemistry, School of Medicine, Southern Illinois University, Carbondale 62901 4413, USA
Biochemistry 34:10063-77. 1995..Differential scanning calorimetry demonstrates that the native protein is extremely thermostable and unfolds reversibly at pH 6.0 with a Tm of approximately 100 degrees C, while the recombinant protein unfolds at 92.7 degrees C...
Positive ion electrospray ionization mass spectrometry of double-stranded DNA/drug complexesR Gupta
Department of Chemistry, University of Wollongong, NSW 2522, Australia
Rapid Commun Mass Spectrom 15:2472-80. 2001..This is in agreement with solution studies and with other recent gas phase results. These data contrast with many earlier ESI-MS studies of dsDNA and DNA/drug complexes in which ions from ssDNA are also normally observed...
Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolateS S Kanwar
Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India
Protein Expr Purif 46:421-8. 2006..The lipase had a Vmax and Km of 0.44 mmol mg(-1)min(-1) and 28 mM for hydrolysis of pNPP, and 0.7 mmol mg(-1)min(-1) and 32 mM for hydrolysis of pNPC, respectively...
Effect of ethanol variation on the internal environment of sol-gel bulk and thin films with agingR Gupta
Division of Biocybernetics, Institute of Nuclear Medicine and Allied Sciences, Delhi 110 054, India
Biosens Bioelectron 21:549-56. 2005..These results are likely to have strong implications in designing the internal environment for applications in biosensors...
Identification of serine protease as a major allergen of Curvularia lunataR Gupta
Institute of Genomics and Integrative Biology, Delhi, India
Allergy 59:421-7. 2004..CONCLUSION: A major glycoprotein allergen Cur l 1 was purified to homogeneity from C. lunata. Amino terminal sequence and biochemical assays identified it as a serine protease...
Molecular characterization of a tyrosine-specific protein phosphatase encoded by a stress-responsive gene in ArabidopsisQ Xu
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
Plant Cell 10:849-57. 1998..In summary, we have identified AtPTP1 as a tyrosine-specific protein phosphatase that may function in stress responses of higher plants...
Cloning, expression, crystallization and preliminary X-ray analysis of the DNA-binding protein Sso10a from Sulfolobus solfataricusM J Teale
Chemistry Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA
Acta Crystallogr D Biol Crystallogr 59:1320-2. 2003..9 A(3) Da(-1) and the solvent content is approximately 57%. Complete X-ray diffraction native data were collected from a single crystal and processed to 2.15 A...
Prediction of human protein function according to Gene Ontology categoriesL J Jensen
Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, The Technical University of Denmark, DK 2800 Lyngby, Denmark
Bioinformatics 19:635-42. 2003..This allows for prediction of the function for orphan proteins where no homologs can be found. Using this method we propose two novel receptors in the human genome, and further demonstrate chromosomal clustering of related proteins...
Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPAG Mer
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Cell 103:449-56. 2000..Our findings support a hand-off model for the assembly and coordination of different components of the DNA repair machinery...
Defining the learning curve for the Focused Abdominal Sonogram for Trauma (FAST) examination: implications for credentialingV H Gracias
Division of Traumatology and Surgical Critical Care, The University of Pennsylvania Medical Center, Philadelphia, USA
Am Surg 67:364-8. 2001..The learning curve for FAST starts to flatten out at 30 to 100 examinations. Training and credentialing policies should consider these findings to optimize patient care...
Environmental effect on the reoccurrence of Alternaria alternata on Trianthema portulacastrumR Gupta
Applied Mycology Lab, University of Delhi, India
J Environ Biol 22:83-6. 2001..Pathogen Alternaria alternata was observed on this host for three years continuously i.e. 1997 to 1999. The incidence of the pathogen depended on rainfall and temperature...
Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tractM A Palladino
Department of Biology, Monmouth University, West Long Branch, NJ 07764, USA
Biol Reprod 76:958-64. 2007..The abundant expression of a majority of TLR family members together with expression of TLR adaptors and activation targets provides strong evidence that TLRs play important roles in innate immunity of the male reproductive tract...
