Ravi Bhushan

Summary

Country: India

Publications

  1. ncbi request reprint Indirect resolution of enantiomers of penicillamine by TLC and HPLC using Marfey's reagent and its variants
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 21:1064-8. 2007
  2. doi request reprint High-performance liquid chromatographic enantioseparation of (RS)-bupropion using isothiocyanate-based chiral derivatizing reagents
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 27:956-9. 2013
  3. doi request reprint Application of optically pure amines as chiral auxiliaries to develop trichloro-s-triazine-based new chiral derivatizing reagents for reversed-phase high-performance liquid chromatographic enantioseparation of DL-selenomethionine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 27:968-73. 2013
  4. doi request reprint Indirect enantioseparation of proteinogenic amino acids using naproxen-based chiral derivatizing reagent and HPLC
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 27:750-6. 2013
  5. doi request reprint (S)-Naproxen as a platform to develop chiral derivatizing reagent for reversed-phase high-performance liquid chromatographic enantioseparation of analytes having a carbonyl functional group
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:1582-8. 2012
  6. doi request reprint Amino acids as chiral selectors in enantioresolution by liquid chromatography
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, India
    Biomed Chromatogr 26:962-71. 2012
  7. doi request reprint HPLC enantioresolution of (R,S)-baclofen using three newly synthesized dichloro-s-triazine reagents having amines and five others having amino acids as chiral auxiliaries
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:743-8. 2012
  8. doi request reprint Application of amino acid amides as chiral auxiliaries in difluoro dinitro benzene and cyanuric chloride moieties for high-performance liquid-chromatographic enantioseparation of selenomethionine and its mixture with methionine and cysteine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Amino Acids 42:1417-23. 2012
  9. doi request reprint Application of cyanuric chloride-based six new chiral derivatizing reagents having amino acids and amino acid amides as chiral auxiliaries for enantioresolution of proteinogenic amino acids by reversed-phase high-performance liquid chromatography
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Amino Acids 42:1371-8. 2012
  10. doi request reprint Validated high-performance liquid chromatographic enantioseparation of selenomethionine using isothiocyanate based chiral derivatizing reagents
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:471-5. 2012

Collaborators

Detail Information

Publications66

  1. ncbi request reprint Indirect resolution of enantiomers of penicillamine by TLC and HPLC using Marfey's reagent and its variants
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 21:1064-8. 2007
    ..The method was validated for linearity, repeatability, limit of detection and limit of quantification...
  2. doi request reprint High-performance liquid chromatographic enantioseparation of (RS)-bupropion using isothiocyanate-based chiral derivatizing reagents
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 27:956-9. 2013
    ..Derivatization conditions were optimized and the method was validated for accuracy, precision and limit of detection. The limit of detection was found to be 0.040-0.043 µg/mL for each of the diastereomers prepared with (S)-NEIT...
  3. doi request reprint Application of optically pure amines as chiral auxiliaries to develop trichloro-s-triazine-based new chiral derivatizing reagents for reversed-phase high-performance liquid chromatographic enantioseparation of DL-selenomethionine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 27:968-73. 2013
    ..The separation behaviors in terms of retention times and resolutions were compared. The separation method was validated for limit of detection, linearity, accuracy, precision, and recovery...
  4. doi request reprint Indirect enantioseparation of proteinogenic amino acids using naproxen-based chiral derivatizing reagent and HPLC
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 27:750-6. 2013
    ..Detection was carried out at 231 nm and sharp peaks were obtained. The method was validated for accuracy, precision and limit of detection...
  5. doi request reprint (S)-Naproxen as a platform to develop chiral derivatizing reagent for reversed-phase high-performance liquid chromatographic enantioseparation of analytes having a carbonyl functional group
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:1582-8. 2012
    ..The diastereomers were detected at 231 nm. The method was validated for accuracy, precision, and limit of detection (LOD). For a series of hydrazones the LOD was found to be in the range 1.62-1.65 pmol/mL...
  6. doi request reprint Amino acids as chiral selectors in enantioresolution by liquid chromatography
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, India
    Biomed Chromatogr 26:962-71. 2012
    ..Enantiomeric resolution of β-blockers, nonsteroidal anti-inflammatories, amino acids (and their derivatives) and certain other compounds is discussed...
  7. doi request reprint HPLC enantioresolution of (R,S)-baclofen using three newly synthesized dichloro-s-triazine reagents having amines and five others having amino acids as chiral auxiliaries
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:743-8. 2012
    ..Chromatographic data obtained for the two sets of diastereomers were compared among themselves and among the two groups. The method was validated for limit of detection, linearity, accuracy and precision...
  8. doi request reprint Application of amino acid amides as chiral auxiliaries in difluoro dinitro benzene and cyanuric chloride moieties for high-performance liquid-chromatographic enantioseparation of selenomethionine and its mixture with methionine and cysteine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Amino Acids 42:1417-23. 2012
    ..TFA (0.1%)--MeCN in different compositions. The method was validated for accuracy, precision, and limit of detection...
  9. doi request reprint Application of cyanuric chloride-based six new chiral derivatizing reagents having amino acids and amino acid amides as chiral auxiliaries for enantioresolution of proteinogenic amino acids by reversed-phase high-performance liquid chromatography
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Amino Acids 42:1371-8. 2012
    ....
  10. doi request reprint Validated high-performance liquid chromatographic enantioseparation of selenomethionine using isothiocyanate based chiral derivatizing reagents
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:471-5. 2012
    ..The method was validated for accuracy, precision and limit of detection. The mechanism of separation is also discussed...
  11. doi request reprint Analytical and preparative enantioseparation of DL-penicillamine and DL-cysteine by high-performance liquid chromatography on alpha-acid glycoprotein and beta-cyclodextrin columns using ninhydrin as a reversible tagging reagent
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    J Chromatogr A 1216:3413-7. 2009
    ..The detection was carried out using photodiode array detection system at 231 nm. The limits of detection were found to be 0.01% and 0.004% for spirothiazolidine carboxylic acid and DNP derivatives, respectively...
  12. doi request reprint Reversed-phase high performance liquid chromatographic separation of diastereomers of beta-amino alcohols and microwave assisted synthesis of Marfey's reagent, its chiral variants and diastereomers
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    J Chromatogr A 1216:2592-6. 2009
    ..In general, amino acid variants of MR provided better separation of diastereomers in comparison to amino acid amide variants. The method was also found successful for the separation of 20 diastereomers from a mixture...
  13. doi request reprint Synthesis of succinimidyl-(S)-naproxen ester and its application for indirect enantioresolution of penicillamine by reversed-phase high-performance liquid chromatography
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1209:174-8. 2008
    ..0, 5mM)-acetonitrile (linear gradient (30min) of acetonitrile from 30 to 70%). Excellent separation was achieved with gradient mobile phase. The detection limit was at pmol level...
  14. doi request reprint Reversed-phase liquid chromatographic determination of enantiomers of atenolol in rat plasma using derivatization with Marfey's reagent
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 23:787-91. 2009
    ..It was shown that the R-isomer eluted before the S-isomer. The method was validated for linearity, repeatability, limits of detection and limit of quantification (LOQ). Recovery of ATE at LOQ was 92.8% for (R)-ATE and 92.6% for (S)-ATE...
  15. doi request reprint Analysis of multicomponent mixture and simultaneous enantioresolution of proteinogenic and non-proteinogenic amino acids by reversed-phase high-performance liquid chromatography using chiral variants of Sanger's reagent
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Anal Bioanal Chem 394:1697-705. 2009
    ..The method was validated for accuracy, precision, limit of detection (LOD), and limit of quantification. LOD is 0.001% impurity of D-enantiomer...
  16. doi request reprint Reversed-phase high-performance liquid chromatographic enantioresolution of six beta-blockers using dinitrophenyl-L-Pro-N-hydroxysuccinimide ester, N-succinimidyl-(S)-2-(6-methoxynaphth-2-yl) propionate and twelve variants of Sanger's reagent as chiral de
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 23:1291-9. 2009
    ..The diastereomers were separated by RP-HPLC. The method was validated for linearity, accuracy, limit of detection and limit of quantification...
  17. doi request reprint Synthesis of dinitrophenyl-L-Pro-N-hydroxysuccinimide ester and four new variants of Sanger's reagent having chiral amines and their application for enantioresolution of mexiletine using reversed-phase high-performance liquid chromatography
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    J Chromatogr A 1216:5769-73. 2009
    ..The method was validated for linearity, accuracy, limit of detection (LOD) and limit of quantification (LOQ)...
  18. doi request reprint Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B12 hydrol
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1216:7941-5. 2009
    ..Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B(12) hydrolysate...
  19. doi request reprint Enantioresolution of dl-penicillamine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 24:66-82. 2010
    ..In addition, application of PenA and its derivatives as chiral selector for enentioresolution of certain other compounds has also been discussed...
  20. doi request reprint Different approaches of impregnation for resolution of enantiomers of atenolol, propranolol and salbutamol using Cu(II)-L-amino acid complexes for ligand exchange on commercial thin layer chromatographic plates
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1217:1395-8. 2010
    ..The detection limit is 0.18 microg for each enantiomer...
  21. doi request reprint Application of (S)-N-(4-Nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as a chiral derivatizing reagent for reversed-phase high-performance liquid chromatographic separation of diastereomers of amino alcohols, non-protein amino acids, and PenA
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology, Roorkee, 247667, India
    Amino Acids 39:549-54. 2010
    ..The detection was at 205 nm and sharp peaks were obtained. The reagent used is comparatively economic than the other derivatizing reagents. Method validation was also done...
  22. doi request reprint Reversed-phase liquid chromatographic resolution of diastereomers of protein and non-protein amino acids prepared with newly synthesized chiral derivatizing reagents based on cyanuric chloride
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Amino Acids 40:403-9. 2011
    ..The reaction conditions were optimized for the synthesis of diastereomers using one MCT reagent. The separation method was validated for limit of detection, linearity, accuracy, precision, and recovery...
  23. doi request reprint Indirect enantioresolution of (R,S)-mexiletine by reversed-phase high-performance liquid chromatography via diastereomerization with [(S,S)-O,O'-di-p-toluoyl tartaric acid anhydride], (S)-naproxen and nine chiral reagents synthesized as variants of Marfey
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 25:398-404. 2011
    ..The method was validated for linearity, accuracy, limit of detection and limit of quantification. The limit of detection was found in the range of 10-30  pmol...
  24. doi request reprint Indirect reversed-phase high-performance liquid chromatographic and direct thin-layer chromatographic enantioresolution of (R,S)-Cinacalcet
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, India
    Biomed Chromatogr 25:674-9. 2011
    ..The limit of detection was found to be 60 pmol in HPLC while in TLC it was found to be in the range of 0.26-0.28 µg for each enantiomers...
  25. doi request reprint Microwave-assisted synthesis and reversed-phase high-performance liquid chromatographic separation of diastereomers of (R,S)-baclofen using ten chiral derivatizing reagents designed from trichloro-s-triazine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    J Chromatogr A 1217:6382-7. 2010
    ..52-0.75% and 0.64-1.32%, respectively. The recovery was 97.2-98.9% for (R)- and 97.0-98.9% for (S)-baclofen. The limit of detection was 1.63 ng/ml and 1.52 ng/ml for (R)- and (S)-baclofen, respectively...
  26. doi request reprint High-performance liquid chromatographic enantioseparation of (R,S)-fluoxetine using Marfey's reagent and (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as chiral derivatizing reagents along with direct thin-layer chromatographic resolutio
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 24:1152-8. 2010
    ..The diastereomers were separated using RP-HPLC. The effect of flow rate and TFA concentration on resolution was studied. The diastereomers obtained by derivatization with FDNP-L-Met-NH₂ were also separated by RP-TLC...
  27. doi request reprint Reversed-phase high-performance liquid chromatographic separation of diastereomers of (R,S)-mexiletine prepared by microwave irradiation with four new chiral derivatizing reagents based on trichloro-s-triazine having amino acids as chiral auxiliaries and
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1217:7669-76. 2010
    ..The method was validated for limit of detection, linearity, accuracy and precision...
  28. doi request reprint Synthesis of (S)-naproxen-benzotriazole and its application as chiral derivatizing reagent for microwave-assisted synthesis and indirect high performance liquid chromatographic separation of diastereomers of penicillamine, cysteine and homocysteine
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1218:3648-53. 2011
    ..5)-acetonitrile (30-65% within 30 min). The method was validated for accuracy, precision, and limit of detection...
  29. doi request reprint Enantioresolution of five β-blockers by reversed-phase high-performance liquid chromatography using fifteen chiral derivatizing reagents having amino acids or their amides as chiral auxiliaries on a cyanuric chloride platform
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 26:239-46. 2012
    ..e. amino acids or amino acid amides) and achiral substituents (i.e. chlorine or methoxy group) in the CDRs. The method was validated for detection limit, linearity, accuracy and precision...
  30. doi request reprint Subunit structure of glycinin and its molecular species based on RP-HPLC, gel electrophoresis and SEC studies
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 22:1296-303. 2008
    ..Glycinin and glycinin II were found to have 12 subunits each while glycinin I showed six subunits. Molecular weight and weight ratio in each case were also determined...
  31. ncbi request reprint Resolution of (+/-)-ibuprofen using (-)-brucine as a chiral selector by thin layer chromatography
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 18:838-40. 2004
    ..Spots were located in an iodine chamber. The detection limit was 4.9 microg. The effect of concentration of the chiral selector, temperature and pH on resolution has been studied...
  32. ncbi request reprint Marfey's reagent for chiral amino acid analysis: a review
    R Bhushan
    Interdisciplinary Research Center IFZ, Justus Liebig Universitat Giessen, Giessen, Germany
    Amino Acids 27:231-47. 2004
    ..Use of MR for preparing CSPs for direct enantiomeric resolution has also been included...
  33. ncbi request reprint Thin-layer chromatography separation of enantiomers of verapamil using macrocyclic antibiotic as a chiral selector
    R Bhushan
    Department of Chemistry, Indian Institute of Technology, Roorkee 247 667, India
    Biomed Chromatogr 19:474-8. 2005
    ..The effects of chiral selector, temperature and pH on resolution were also studied. The spots were detected with iodine vapors and the detection limit was found to be 0.074 microg of each enantiomers...
  34. ncbi request reprint Liquid chromatographic separation and UV determination of certain antihypertensive agents
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 20:217-24. 2006
    ..Recovery was in the range 82-93%. Two combination of drugs, viz. amlodipine+ramipril and amlodipine+enalapril, were separated by the three modes of liquid chromatography. The percentage recovery was in the range 80-92% by open column...
  35. ncbi request reprint HPLC resolution of thioridazine enantiomers from pharmaceutical dosage form using cyclodextrin-based chiral stationary phase
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    J Chromatogr B Analyt Technol Biomed Life Sci 837:133-7. 2006
    ..The detection limit was found to be 10 microg (5 microg of each enantiomer). The enantiomeric purity of each of the resolved isomers was verified by optical rotation...
  36. ncbi request reprint Reversed-phase high-performance liquid chromatographic, gel electrophoretic and size exclusion chromatographic studies of subunit structure of arachin and its molecular species
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, India
    Biomed Chromatogr 20:561-8. 2006
    ..The subunits of arachin I were allowed to reconstitute by removing SDS. Eight combinations were tried for studying the reconstitution pattern. Molecular weight and weight ratio in each case were also determined...
  37. ncbi request reprint Reversed-phase high-performance liquid chromatographic, size exclusion chromatographic and polyacrylamide gel electrophoretic studies of glycinin: evidence for molecular species and their association-dissociation
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 21:1245-51. 2007
    ..Glycinin II also did not dissociate under varying conditions of time, pH and ionic strength of buffer. Various species so dissociated were isolated, purified and characterized...
  38. doi request reprint Synthesis of chiral hydrazine reagents and their application for liquid chromatographic separation of carbonyl compounds via diastereomer formation
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1190:86-94. 2008
    ..Studies related to effects of structural modification in reagents and analytes on chromatographic behavior of diastereomers were also analyzed...
  39. doi request reprint Indirect resolution of baclofen enantiomers from pharmaceutical dosage form by reversed-phase liquid chromatography after derivatization with Marfey's reagent and its structural variants
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 22:906-11. 2008
    ..The effect of flow rate, acetonitrile content and TFA concentration on resolution was studied. The method was validated for linearity, repeatability, limit of detection and limit of quantification...
  40. doi request reprint Indirect enantioseparation of alpha-amino acids by reversed-phase liquid chromatography using new chiral derivatizing reagents synthesized from s-triazine chloride
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr A 1201:35-42. 2008
    ..Effects of structural modifications in reagents on chromatographic properties were investigated. Separation mechanism of diastereomers was proposed in light of both MCT and DCT reagents...
  41. doi request reprint Chromatographic separation of enantiomers of non-protein alpha-amino acids after derivatization with Marfey's reagent and its four variants
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Amino Acids 36:571-9. 2009
    ..The effects of TFA concentration, flow rate and run time on HPLC separation were studied...
  42. doi request reprint Direct enantiomeric TLC resolution of dl-penicillamine using (R)-mandelic acid and l-tartaric acid as chiral impregnating reagents and as chiral mobile phase additive
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    Biomed Chromatogr 22:1237-42. 2008
    ..The spots were detected with iodine vapors and the detection limits were found to be 0.12 microg for each enantiomer of penicillamine with L-tartaric acid, under both the conditions, and 0.11 microg with (R)-mandelic acid...
  43. ncbi request reprint Liquid chromatographic analysis of certain commercial formulations for non-opioid analgesics
    R Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India
    Biomed Chromatogr 21:1284-90. 2007
    ..These were extracted, isolated, purified and recrystallized and were characterized by melting point, lambda(max) and IR. Quantitative determination was carried out using HPLC and TLC supplemented with UV spectrophotometry...
  44. doi request reprint HPLC enantioseparation of racemic bupropion, baclofen and etodolac: modification of conventional ligand exchange approach by pre-column formation of chiral ligand exchange complexes
    Manisha Singh
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 30:1728-1732. 2016
    ..Baseline separation of the two enantiomers was obtained with a resolution of 6.63 in <15 min. Copyright © 2016 John Wiley & Sons, Ltd...
  45. ncbi request reprint Direct enantiomeric resolution of (+/-)-atenolol, (+/-)-metoprolol, and (+/-)-propranolol by impregnated TLC using L-aspartic acid as chiral selector
    R Bhushan
    Department of Chemistry, Indian Insitute of Technology, Roorkee 247 667, India
    Biomed Chromatogr 17:226-30. 2003
    ..The spots were detected with iodine and the detection limits were found to be 0.26 microg for atenolol and 0.23 microg for each of metoprolol and propranolol as racemate...
  46. ncbi request reprint Direct resolution of (+/-)-ephedrine and atropine into their enantiomers by impregnated TLC
    R Bhushan
    Department of Chemistry, University of Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 15:151-4. 2001
    ..The effects of concentration of the impregnating reagent, temperature and pH on resolution have been studied...
  47. pmc Resolution of Enantiomers of (RS)-Baclofen by Ligand-Exchange Thin-Layer Chromatography
    Manisha Singh
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr Sci 54:842-6. 2016
    ..L-Trp proved to be a good ligand using a common mobile phase in each case...
  48. doi request reprint Direct TLC resolution of atenolol and propranolol into their enantiomers using three different chiral selectors as impregnating reagents
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 22:1028-34. 2008
    ..Spots were detected using iodine vapour. The TLC method was validated for linearity, limit of detection and limit of quantification. The influence of pH, temperature and concentration of chiral selector was studied...
  49. ncbi request reprint Resolution of enantiomers of DL-amino acids on silica gel plates impregnated with optically pure (-)-quinine
    R Bhushan
    Department of Chemistry, University of Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 15:433-6. 2001
    ..9 and 3.7 microg. The effects of concentration of impregnating reagent, temperature and pH on resolution of enantiomers have been studied in details...
  50. ncbi request reprint Separation of amino acids, their derivatives and enantiomers by impregnated TLC
    R Bhushan
    Department of Chemistry, University of Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 15:155-65. 2001
    ....
  51. ncbi request reprint Direct thin layer chromatography enantioresolution of some basic DL-amino acids using a pharmaceutical industry waste as chiral impregnating reagent
    R Bhushan
    Department of Chemistry, University of Roorkee, India
    J Pharm Biomed Anal 21:1143-7. 2000
    ..Different combinations of acetonitrile-methanol-water were found to be successful in resolving the DL-amino acids. The spots were detected by ninhydrin (0.2% in acetone) and the detection limit was 0.66 microg...
  52. ncbi request reprint Sediment deposition rates on the continental margins of the eastern Arabian Sea using 210Pb, 137Cs and 14C
    B L Somayajulu
    Physical Research Laboratory, Ahmedabad, India
    Sci Total Environ 237:429-39. 1999
    ..004 to 0.13 cm/year. For long-term chronology (< or = 50,000 years) AMS dating of well-cleaned planktonic foraminifera is most suited...
  53. ncbi request reprint Liquid chromatographic separation of some PTH-amino acids
    R Bhushan
    Department of Chemistry, University of Roorkee, India
    Biomed Chromatogr 12:322-5. 1998
    ..Acetonitrile and sodium acetate buffer of pH 4.0 was used for reversed phase chromatography while for normal phase TLC combinations of chloroform-acetonitrile and chloroform-tetrahydrofuran were applied...
  54. doi request reprint (RS)-Propranolol: enantioseparation by HPLC using newly synthesized (S)-levofloxacin-based reagent, absolute configuration of diastereomers and recovery of native enantiomers by detagging
    Shiv Alwera
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 30:1223-33. 2016
    ..With the systematic application of various analytical techniques, absolute configuration of the diastereomers (and the native enantiomers) of (RS)-propranolol was established. Copyright © 2016 John Wiley & Sons, Ltd. ..
  55. doi request reprint Analytical and semi-preparative enantioresolution of (RS)-ketorolac from pharmaceutical formulation and in human plasma by HPLC
    Manohar Lal
    Department of Chemistry, Lovely Professional University, Phagwara Punjab, 144411, India
    Biomed Chromatogr 30:1526-34. 2016
    ..The limit of detection and quantitation values were 5 and 15.5 ng/mL for each enantiomer of (RS)-Ket, respectively. Copyright © 2016 John Wiley & Sons, Ltd. ..
  56. doi request reprint Liquid chromatographic enantioseparation of three beta-adrenolytics using new derivatizing reagents synthesized from (S)-ketoprofen and confirmation of configuration of diastereomers
    Shiv Alwera
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 30:1772-1781. 2016
    ..RP HPLC conditions for separation were optimized and the separation method was validated. The limit of detection values were 0.308 and 0.302 ng mL-1 . Copyright © 2016 John Wiley & Sons, Ltd...
  57. ncbi request reprint Improved thin layer chromatographic resolution of PTH amino acids with some new solvent systems
    R Bhushan
    Department of Chemistry, University of Roorkee, India
    Biomed Chromatogr 8:69-72. 1994
    ..3:8, v/v), for the resolution of a mixture of 18 PTH amino acids have been reported. Using these solvent systems, various combinations of PTH amino acids which had previously posed resolution problems have been resolved and identified...
  58. ncbi request reprint Improved TLC systems for rapid resolution of phenyl thiohydantoin amino acids
    R Bhushan
    Department of Chemistry, University of Roorkee, India
    Biomed Chromatogr 3:43-5. 1989
    ..Two more solvent systems, viz chloroform + acetic acid (27:3, v/v) and chloroform + methanol (30:4, v/v), are developed to resolve phenylthiodantoin derivatives of aspartic and glutamic acids...
  59. doi request reprint A Rapid, Robust and Ultra-Sensitive HPLC Enantioseparation of β-Amino Alcohols
    Rituraj Dubey
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India
    J Chromatogr Sci 53:1366-72. 2015
    ..Excellent resolution to a pmol detection limit level was achieved. The developed HPLC method for enantioseparation of β-amino alcohols is the first-ever report of its own kind having such a high detection sensitivity. ..
  60. ncbi request reprint Enantiomeric Resolution of (RS)-Naproxen and application of (S)-Naproxen in the direct and indirect enantioseparation of racemic compounds by liquid chromatography: A review
    Sonika Batra
    Department of Chemistry, Indian Institute of Technology Roorkee, 247667, India
    Curr Med Chem . 2016
    ....
  61. doi request reprint Resolution and isolation of enantiomers of (±)-isoxsuprine using thin silica gel layers impregnated with L-glutamic acid, comparison of separation of its diastereomers prepared with chiral derivatizing reagents having L-amino acids as chiral auxiliaries
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 29:357-65. 2015
    ..The conditions of derivatization and chromatographic separation were optimized. The method was validated for accuracy, precision, limit of detection and limit of quantification...
  62. doi request reprint Amino acids as chiral auxiliaries in cyanuric chloride-based chiral derivatizing agents for enantioseparation by liquid chromatography
    Sonika Batra
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 28:1532-46. 2014
    ..Derivatization methods are particularly required when a chromophore is to be introduced in low UV absorbing molecules, for their detection...
  63. doi request reprint Liquid chromatographic enantioseparation of (RS)-mexiletine and (RS)-fluoxetine using chiral derivatizing reagents synthesized with (S)-naproxen moiety
    Sonika Batra
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 28:815-25. 2014
    ..The conditions for derivatization and chromatographic separation were optimized. The method was validated for linearity, repeatability, limit of detection and limit of quantification...
  64. doi request reprint Indirect enantioseparation of selenomethionine by reversed-phase high-performance liquid chromatography using a newly synthesized chiral derivatizing reagent based on (S)-naproxen moiety
    Ravi Bhushan
    Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India
    Biomed Chromatogr 28:106-11. 2014
    ..Optimized structures of the two diastereomers were drawn using the Gaussian 09 Rev. A.02 program and hybrid density functional B3LYP with 6-31G basis set to explain the separation mechanism...
  65. ncbi request reprint Separation of cephalosporins on thin silica gel layers impregnated with transition metal ions and by reversed-phase TLC
    Ravi Bhushan
    Department of Chemistry, University of Roorkee, Roorkee 247 667, India
    Biomed Chromatogr 16:165-74. 2002
    ..Activation time of thin layer plates impregnated with 0.1% FeSO(4) was found to affect both hR(F) values and resolution of cephalosporins. New solvent systems are reported for both normal phase and reversed-phase TLC...
  66. ncbi request reprint Rapid vertical mixing rates in deep waters of the Andaman Basin
    Koushik Dutta
    Planetary and Geosciences Division, Physical Research Laboratory, Navrangpura, Ahmedabad 380 009, India
    Sci Total Environ 384:401-8. 2007
    ..In addition, Andaman Basin is underlain with a young crust and is known for its high heat flow, which also could contribute to the high vertical mixing...