Bela Gyurcsik

Summary

Affiliation: University of Szeged
Location: Szeged, Hungary
URL: http://www.staff.u-szeged.hu/~gyurcsik/
Summary:
Bioinorganic chemistry, artificial metallonucleases.

Publications

  1. Gyurcsik B, Czene A. Towards artificial metallonucleases for gene therapy: recent advances and new perspectives. Future Med Chem. 2011;3:1935-66 pubmed publisher
    ..The results collected provide useful guidance for engineering highly controlled enzymes for use in gene therapy. ..
  2. Lakatos A, Gyurcsik B, Nagy N, Csendes Z, Wéber E, Fülöp L, et al. Histidine-rich branched peptides as Cu(II) and Zn(II) chelators with potential therapeutic application in Alzheimer's disease. Dalton Trans. 2012;41:1713-26 pubmed publisher
    ..Our results suggest that branched peptides containing potential metal-binding sites may be suitable metal chelators for reducing the risk of amyloid plaque formation in Alzheimer's disease. ..
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    Gyurcsik B, Vosekalna I, Larsen E. CD spectroscopic study on the speciation and solution structure of copper(II) complexes of some tripeptides in combination with potentiometric and spectrophotometric results. J Inorg Biochem. 2001;85:89-98 pubmed
    ..The CD spectra strongly support the coordination also of the C-terminal part of the peptide in the CuLH(-1) complex of GGA, and even the deprotonation of the Ala peptide group instead of that belonging to Gly cannot be excluded. ..
  4. Jancsó A, Szunyogh D, Larsen F, Thulstrup P, Christensen N, Gyurcsik B, et al. Towards the role of metal ions in the structural variability of proteins: CdII speciation of a metal ion binding loop motif. Metallomics. 2011;3:1331-9 pubmed publisher
    ..In an equilibrium of several co-existing species the peptide is exchanging between a number of structures also in its metal ion bound state(s), as indicated by NMR and PAC data. ..
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    Jancso A, Paksi Z, Jakab N, Gyurcsik B, Rockenbauer A, Gajda T. Solution chemical properties and catecholase-like activity of the copper(II)-Ac-His-His-Gly-His-OH system, a relevant functional model for copper containing oxidases. Dalton Trans. 2005;:3187-94 pubmed
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    Sebek J, Gyurcsik B, Sebestik J, Kejík Z, Bednarova L, Bour P. Interpretation of synchrotron radiation circular dichroism spectra of anionic, cationic, and zwitterionic dialanine forms. J Phys Chem A. 2007;111:2750-60 pubmed
    ..Future peptide and protein conformational studies based on circular dichroic spectroscopy can be reliable only if such effects of molecular dynamics, solvent structure, and polar solvent-solute interactions are taken into account. ..
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    Jakab N, Gyurcsik B, Kortvelyesi T, Vosekalna I, Jensen J, Larsen E. Design of histidine containing peptides for better understanding of their coordination mode toward copper(II) by CD spectroscopy. J Inorg Biochem. 2007;101:1376-85 pubmed
    ..CD spectra strongly suggest the participation of the imidazole nitrogen both in the Cu(2)H(-2)L(2) and CuH(-2)L complexes. ..
  8. Jancsó A, Kolozsi A, Gyurcsik B, Nagy N, Gajda T. Probing the Cu(2+) and Zn(2+) binding affinity of histidine-rich glycoprotein. J Inorg Biochem. 2009;103:1634-43 pubmed publisher
    ..Whether the observed cooperativity has similarities to the cooperative metal binding feature of HRG or the two phenomena have different sources is a question yet to be clarified. ..
  9. Kolozsi A, Lakatos A, Galbács G, Madsen A, Larsen E, Gyurcsik B. A pH-metric, UV, NMR, and X-ray crystallographic study on arsenous acid reacting with dithioerythritol. Inorg Chem. 2008;47:3832-40 pubmed publisher
    ..In such alkaline solutions, most probably, rearrangement of the bonding scheme occurs, resulting in ligands being bound to the arsenic(III) through the oxygen donor atoms. ..

Detail Information

Publications24

  1. request reprint
    Gyurcsik B. [Modeling of potential metal binding sites of glycoproteins]. Acta Pharm Hung. 2000;70:146-59 pubmed
  2. Nagy G, Gyurcsik B, Hoffmann E, Kortvelyesi T. Theoretical design of a specific DNA-Zinc-finger protein interaction with semi-empirical quantum chemical methods. J Mol Graph Model. 2011;29:928-34 pubmed publisher
    ..The stacking interactions between the nucleic bases were also characterized in the DNA duplexes. ..
  3. Szabó Plánka T, Gyurcsik B, Pálinkó I, Nagy N, Rockenbauer A, Sípoš R, et al. Effect of fluorine position on the coordinating ability of fluorosalicylic acids--an experimental study complemented with computations. J Inorg Biochem. 2011;105:75-83 pubmed publisher
    ..For the interpretation of these findings, ab initio and semi-empirical quantum chemical calculations were carried out for the ligand molecules, isolated and surrounded by water molecules, respectively. ..
  4. Kolozsi A, Vosekalna I, Martinek T, Larsen E, Gyurcsik B. Copper(II) and zinc(II) ion binding properties of a MAP type branched ligand with histidines as surface functionalities. Dalton Trans. 2009;:5647-54 pubmed publisher
    ..The second metal ion is probably surrounded by two amide nitrogens and two others either from amino or imidazole donor groups. ..
  5. Jakab I, Lorincz O, Jancsó A, Gajda T, Gyurcsik B. Approaching the minimal metal ion binding peptide for structural and functional metalloenzyme mimicking. Dalton Trans. 2008;:6987-95 pubmed publisher
  6. Jakab N, Jancsó A, Gajda T, Gyurcsik B, Rockenbauer A. Copper(II), nickel(II) and zinc(II) complexes of N-acetyl-His-Pro-His-His-NH2: equilibria, solution structure and enzyme mimicking. J Inorg Biochem. 2008;102:1438-48 pubmed publisher
    ..At pH 10 an octahedral NiH(-2)L species is present at first, which transforms slowly to a yellow square planar complex. ..
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    Szabó Plánka T, Gyurcsik B, Nagy N, Rockenbauer A, Sípoš R, Sima J, et al. Complexation of 5-fluorosalicylic acid with copper(II): a pH-potentiometric, UV-vis spectroscopic, and electron spin resonance study by the two-dimensional simulation of spectra. J Inorg Biochem. 2008;102:101-9 pubmed
    ..In frozen solutions, the ESR analysis revealed a slight rhombic distortion of coordination polyhedra for the latter three species. ..
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    Gyurcsik B, Haruki H, Takahashi T, Mihara H, Nagata K. Binding modes of the precursor of adenovirus major core protein VII to DNA and template activating factor I: implication for the mechanism of remodeling of the adenovirus chromatin. Biochemistry. 2006;45:303-13 pubmed
    ..This binding mode suggests the formation of the ternary complexes among DNA, protein VII, and TAF-I. On the basis of the binding modes in binary systems, we discussed the remodeling mechanism of the Ad core in early phases of infection. ..
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    Haruki H, Gyurcsik B, Okuwaki M, Nagata K. Ternary complex formation between DNA-adenovirus core protein VII and TAF-Ibeta/SET, an acidic molecular chaperone. FEBS Lett. 2003;555:521-7 pubmed
    ..In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA-protein VII complexes. ..
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    Dörnyei Á, Kilyén M, Kiss T, Gyurcsik B, Laczkó I, Pécsváradi A, et al. The effects of Al(III) speciation on the activity of trypsin. J Inorg Biochem. 2003;97:118-23 pubmed
    ..These results strongly suggest that there is no direct involvement of Al(III) in the enzymatic reactions of trypsin. ..
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    Burger K, Illes J, Gyurcsik B, Gazdag M, Forrai E, Dekany I, et al. Metal ion coordination of macromolecular bioligands: formation of zinc(II) complex of hyaluronic acid. Carbohydr Res. 2001;332:197-207 pubmed
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    Gyurcsik B, Gajda T, Nagy L, Burger K. [Structural and equilibrium studies of the proton and copper(II) complexes of N-D-gluconylglycine]. Acta Pharm Hung. 1993;63:115-27 pubmed
    ..At pH > 9, further base-consuming processes start as an indication of the deprotonation of other alcoholic hydroxy groups of the sugar moiety or the formation of mixed hydroxy complexes. ..
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    Gyurcsik B. Rescue of the activity of HNH nuclease mutants - towards controlled enzymes for gene therapy. Curr Protein Pept Sci. 2016;17:191-7 pubmed
    ..Here we collect the results on the properties of the HNH nucleases that allow for their redesign into enzymes with possible therapeutic applications. ..
  14. Nemeth E, Balogh R, Borsos K, Czene A, Thulstrup P, Gyurcsik B. Intrinsic protein disorder could be overlooked in cocrystallization conditions: An SRCD case study. Protein Sci. 2016;25:1977-1988 pubmed publisher
    ..The growing evidence on the importance of protein intrinsic disorder thus, demands more extensive complementary experiments in solution phase with the unligated form of the protein of interest. ..
  15. Tóth E, May N, Rockenbauer A, Peintler G, Gyurcsik B. Exploring the boundaries of direct detection and characterization of labile isomers - a case study of copper(ii)-dipeptide systems. Dalton Trans. 2017;46:8157-8166 pubmed publisher
    ..This study exemplifies the necessity of using temperature dependent multiple methodologies for a reliable description of similar systems for upstream applications. ..