Peter Varnai

Summary

Affiliation: Semmelweis University
Country: Hungary

Publications

  1. pmc Live cell imaging of phosphoinositides with expressed inositide binding protein domains
    Peter Varnai
    Department of Physiology, Semmelweis University Faculty of Medicine, Budapest, H 1088 Budapest, Puskin utca 9, Hungary, Bethesda, MD 20892, USA
    Methods 46:167-76. 2008
  2. ncbi request reprint Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domains
    Peter Varnai
    Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bldg 49, Rm 6A35, 49 Convent Drive, Bethesda, MD, USA
    Pflugers Arch 455:69-82. 2007
  3. ncbi request reprint Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130
    Peter Varnai
    Endocrinology and Reproduction Research Branch, NICHD National Institutes of Health, 49 Convent Drive, Bldg 49, Bethesda, MD 20892, USA
    J Biol Chem 277:27412-22. 2002
  4. pmc Dependence of STIM1/Orai1-mediated calcium entry on plasma membrane phosphoinositides
    Marek K Korzeniowski
    Sections on molecular signal transduction, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:21027-35. 2009
  5. ncbi request reprint Selective cellular effects of overexpressed pleckstrin-homology domains that recognize PtdIns(3,4,5)P3 suggest their interaction with protein binding partners
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 118:4879-88. 2005
  6. pmc Mapping of the localization of type 1 angiotensin receptor in membrane microdomains using bioluminescence resonance energy transfer-based sensors
    Andras Balla
    Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
    J Biol Chem 287:9090-9. 2012
  7. pmc Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:7859-64. 2005
  8. doi request reprint Allosteric interactions within the AT₁ angiotensin receptor homodimer: role of the conserved DRY motif
    Bence Szalai
    Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary
    Biochem Pharmacol 84:477-85. 2012
  9. pmc Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 175:377-82. 2006
  10. ncbi request reprint The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme
    Sang Bong Lee
    Laboratory of Cell Signaling, NHLI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:24362-71. 2004

Collaborators

Detail Information

Publications30

  1. pmc Live cell imaging of phosphoinositides with expressed inositide binding protein domains
    Peter Varnai
    Department of Physiology, Semmelweis University Faculty of Medicine, Budapest, H 1088 Budapest, Puskin utca 9, Hungary, Bethesda, MD 20892, USA
    Methods 46:167-76. 2008
    ....
  2. ncbi request reprint Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domains
    Peter Varnai
    Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bldg 49, Rm 6A35, 49 Convent Drive, Bethesda, MD, USA
    Pflugers Arch 455:69-82. 2007
    ..In this review, we will summarize our efforts to improve our tools in studying phosphoinositide dynamics and discuss our views on the values of these methods compared to other options currently used or being explored...
  3. ncbi request reprint Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130
    Peter Varnai
    Endocrinology and Reproduction Research Branch, NICHD National Institutes of Health, 49 Convent Drive, Bldg 49, Bethesda, MD 20892, USA
    J Biol Chem 277:27412-22. 2002
    ....
  4. pmc Dependence of STIM1/Orai1-mediated calcium entry on plasma membrane phosphoinositides
    Marek K Korzeniowski
    Sections on molecular signal transduction, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:21027-35. 2009
    ..These results suggest an inositide requirement of Orai1 activation but not STIM1 movements and indicate that PtdIns4P rather than PtdIns(4,5)P2 is a likely determinant of Orai1 channel activity...
  5. ncbi request reprint Selective cellular effects of overexpressed pleckstrin-homology domains that recognize PtdIns(3,4,5)P3 suggest their interaction with protein binding partners
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 118:4879-88. 2005
    ....
  6. pmc Mapping of the localization of type 1 angiotensin receptor in membrane microdomains using bioluminescence resonance energy transfer-based sensors
    Andras Balla
    Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
    J Biol Chem 287:9090-9. 2012
    ....
  7. pmc Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:7859-64. 2005
    ....
  8. doi request reprint Allosteric interactions within the AT₁ angiotensin receptor homodimer: role of the conserved DRY motif
    Bence Szalai
    Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary
    Biochem Pharmacol 84:477-85. 2012
    ..These data suggest that allosteric interactions in the homodimers of AT₁R significantly affect the function of the non-stimulated protomer, and the conserved DRY motif has a crucial role in these interactions...
  9. pmc Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 175:377-82. 2006
    ....
  10. ncbi request reprint The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme
    Sang Bong Lee
    Laboratory of Cell Signaling, NHLI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:24362-71. 2004
    ....
  11. ncbi request reprint Endocytosis of the AT1A angiotensin receptor is independent of ubiquitylation of its cytoplasmic serine/threonine-rich region
    Balázs Mihalik
    Department of Physiology, Faculty of Medicine, Semmelweis University, P O Box 259, H 1444 Budapest, Hungary
    Int J Biochem Cell Biol 35:992-1002. 2003
    ....
  12. ncbi request reprint Visualization and manipulation of plasma membrane-endoplasmic reticulum contact sites indicates the presence of additional molecular components within the STIM1-Orai1 Complex
    Peter Varnai
    Section on Molecular Signal Transduction, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    J Biol Chem 282:29678-90. 2007
    ..These studies are the first to detect juxtaposed areas between the ER and the plasma membrane in live cells, revealing novel details of STIM1-Orai1 interactions...
  13. pmc Maintenance of hormone-sensitive phosphoinositide pools in the plasma membrane requires phosphatidylinositol 4-kinase IIIalpha
    Andras Balla
    Section on Molecular Signal Transduction, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 19:711-21. 2008
    ....
  14. pmc Paracrine transactivation of the CB1 cannabinoid receptor by AT1 angiotensin and other Gq/11 protein-coupled receptors
    Gabor Turu
    Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
    J Biol Chem 284:16914-21. 2009
    ..These data suggest that, in addition to their retrograde neurotransmitter role, endocannabinoids have much broader paracrine mediator functions during activation of G(q/11)-coupled receptors...
  15. pmc Visualization of cellular phosphoinositide pools with GFP-fused protein-domains
    Tamas Balla
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2009
    ..This unit summarizes the design and practical use of these constructs and also reviews important considerations for interpretation of the data obtained by this technique...
  16. pmc Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains
    Lee Ann Cohen
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, and Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 18:2244-53. 2007
    ..This interaction was direct and required both inositol phospholipids and GTP. We propose a model of sequential Arf activation at the PM whereby Arf6-GTP recruits ARNO family GEFs for further activation of other Arf isoforms...
  17. ncbi request reprint Live cell imaging of phosphoinositide dynamics with fluorescent protein domains
    Peter Varnai
    Endocrinology and Reproduction Research Branch, NICHD, National Institutes of Health, Bethesda, MD 20892 4510, USA
    Biochim Biophys Acta 1761:957-67. 2006
    ..The most important value of these debates is that they also challenge our preconceived views of how phosphoinositides regulate cellular functions...
  18. pmc Acute depletion of plasma membrane phosphatidylinositol 4,5-bisphosphate impairs specific steps in endocytosis of the G-protein-coupled receptor
    Dániel J Tóth
    Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary
    J Cell Sci 125:2185-97. 2012
    ..Our data suggest that in the absence of PtdIns(4,5)P(2), these receptors move into clathrin-coated membrane structures, but these are not cleaved efficiently and hence cannot reach the early endosomal compartment...
  19. pmc Demonstration of angiotensin II-induced Ras activation in the trans-Golgi network and endoplasmic reticulum using bioluminescence resonance energy transfer-based biosensors
    Andras Balla
    Department of Physiology, Faculty of Medicine, Semmelweis University, H 1444 Budapest, Hungary
    J Biol Chem 286:5319-27. 2011
    ..These data suggest that AngII can stimulate Ras activity in the TGN and ER with a G protein-dependent mechanism, which does not require β-arrestin-mediated signaling, receptor internalization, and EGF receptor transactivation...
  20. pmc STIM and Orai: the long-awaited constituents of store-operated calcium entry
    Peter Varnai
    Department of Physiology, Semmelweis University Faculty of Medicine, H 1088, Puskin u 9, Budapest, Hungary
    Trends Pharmacol Sci 30:118-28. 2009
    ..Here, we briefly summarize recent developments in this exciting area of Ca(2+) signaling...
  21. pmc Activation of STIM1-Orai1 involves an intramolecular switching mechanism
    Marek K Korzeniowski
    Section on Molecular Signal Transduction, Program for Developmental Neuroscience, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Sci Signal 3:ra82. 2010
    ..This evolutionarily conserved mechanism of STIM1 activation resembles the regulation of protein kinases by intramolecular silencing through pseudosubstrate binding...
  22. ncbi request reprint Visualizing cellular phosphoinositide pools with GFP-fused protein-modules
    Tamas Balla
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Sci STKE 2002:pl3. 2002
    ..Here, we summarize our experience in designing and using these constructs and review our position concerning the interpretation of the data obtained by this technique...
  23. pmc Acute manipulation of Golgi phosphoinositides to assess their importance in cellular trafficking and signaling
    Zsofia Szentpetery
    Section on Molecular Signal Transduction, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:8225-30. 2010
    ..This unique approach will allow further studies on the role of phosphoinositides in endocytic compartments that have evaded detection using the conventional long-term manipulations of inositide kinase and phosphatase activities...
  24. doi request reprint Calcium entry is regulated by Zn2+ in relation to extracellular ionic environment in human airway epithelial cells
    Dóra Hargitai
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Respir Physiol Neurobiol 170:67-75. 2010
    ..In conclusion, Ca(2+) entry is finely regulated by external ionic environment. Therefore, we speculate that properly compiled aerosols could influence efficacy of zinc-based therapy in CF...
  25. pmc The effect of OPA1 on mitochondrial Ca²⁺ signaling
    László Fülöp
    Department of Physiology, Faculty of Medicine, Semmelweis University, Hungarian Academy of Sciences, Budapest, Hungary
    PLoS ONE 6:e25199. 2011
    ..Altogether, our observations reveal the significance of OPA1 in the control of mitochondrial Ca²⁺ metabolism...
  26. pmc Relating underrepresented genomic DNA patterns and tiRNAs: the rule behind the observation and beyond
    Miklos Cserzo
    Department of Physiology, Semmelweis University, Budapest, Tuzolto Street, 37 47, 1094, Hungary, EU
    Biol Direct 5:56. 2010
    ..The exact role of these molecules in gene expression control is mostly unknown at present, while their importance is generally recognized...
  27. ncbi request reprint Mechanisms of angiotensin II-mediated regulation of aldosterone synthase expression in H295R human adrenocortical and rat adrenal glomerulosa cells
    Maria Szekeres
    Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary
    Mol Cell Endocrinol 302:244-53. 2009
    ....
  28. doi request reprint Chronic repeated restraint stress increases prolactin-releasing peptide/tyrosine-hydroxylase ratio with gender-related differences in the rat brain
    Zsuzsanna E Toth
    Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Histology and Embryology of the Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary
    J Neurochem 104:653-66. 2008
    ....
  29. doi request reprint Redox state of the endoplasmic reticulum is controlled by Ero1L-alpha and intraluminal calcium
    Balázs Enyedi
    Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary
    Antioxid Redox Signal 13:721-9. 2010
    ..We also found that calcium mobilization from intracellular stores induces a decrease in ER H(2)O(2) level, suggesting a complex interplay between redox and calcium signaling in the mammalian ER...
  30. ncbi request reprint AT1 receptor blocker-insensitive mutant AT1A angiotensin receptors reveal the presence of G protein-independent signaling in C9 cells
    Laszlo Szidonya
    Department of Physiology, Semmelweis University, Budapest, Hungary
    Biochem Pharmacol 73:1582-92. 2007
    ....