Research Topics
Species | LASZLO CSANADYSummaryAffiliation: Semmelweis University Country: Hungary Publications
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Detail Information
Publications
Application of rate-equilibrium free energy relationship analysis to nonequilibrium ion channel gating mechanismsLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
J Gen Physiol 134:129-36. 2009..Additionally, REFER analysis provides limited information about the transition-state structures for gating schemes that are known to be nonequilibrium cycles...
Electrophysiological, biochemical, and bioinformatic methods for studying CFTR channel gating and its regulationLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
Methods Mol Biol 741:443-69. 2011....- Four Ca2+ ions activate TRPM2 channels by binding in deep crevices near the pore but intracellularly of the gateLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
J Gen Physiol 133:189-203. 2009..Our results suggest that in intact cells that contain micromolar ADPR a single brief puff of Ca(2+) likely triggers prolonged, self-sustained TRPM2 activity... - Strict coupling between CFTR's catalytic cycle and gating of its Cl- ion pore revealed by distributions of open channel burst durationsLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, Budapest H 1094, Hungary
Proc Natl Acad Sci U S A 107:1241-6. 2010.... - Thermodynamics of CFTR channel gating: a spreading conformational change initiates an irreversible gating cycleLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, 1088 Budapest, Hungary
J Gen Physiol 128:523-33. 2006.... - Functional roles of nonconserved structural segments in CFTR's NH2-terminal nucleotide binding domainLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, 1088 Budapest, Hungary
J Gen Physiol 125:43-55. 2005..In contrast, 633+668 channel function was indistinguishable from WT at both macroscopic and microscopic levels. We conclude that neither nonconserved segment is an essential element of PKA- or nucleotide-dependent regulation... - Mutant cycles at CFTR's non-canonical ATP-binding site support little interface separation during gatingAndras Szollosi
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
J Gen Physiol 137:549-62. 2011..These results provide independent support for a gating model in which ATP-bound composite site 1 remains closed throughout the gating cycle... - Preferential phosphorylation of R-domain Serine 768 dampens activation of CFTR channels by PKALASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, Budapest, Germany
J Gen Physiol 125:171-86. 2005..The observed reduced sensitivity to activation by [PKA] imparted by Ser 768 might serve to ensure activation of WT CFTR by strong stimuli while dampening responses to weak signals... - Pore collapse underlies irreversible inactivation of TRPM2 cation channel currentsBalazs Toth
Department of Medical Biochemistry, Semmelweis University, Budapest H 1094, Hungary
Proc Natl Acad Sci U S A 109:13440-5. 2012..The noninactivating TRPM2 variant will be invaluable for gating studies... - Involvement of F1296 and N1303 of CFTR in induced-fit conformational change in response to ATP binding at NBD2Andras Szollosi
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
J Gen Physiol 136:407-23. 2010.... - Antagonistic regulation of native Ca2+- and ATP-sensitive cation channels in brain capillaries by nucleotides and decavanadateLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, and Neurochemical Group of the Hungarian Academy of Sciences, Budapest, Hungary
J Gen Physiol 123:743-57. 2004..Decavanadate now provides a valuable tool for studying native CA-NSC channels and for screening cloned channels... - Ca(2+)- and voltage-dependent gating of Ca(2+)- and ATP-sensitive cationic channels in brain capillary endotheliumLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
Biophys J 85:313-27. 2003..We conclude that the biophysical properties of gating of this channel are remarkably similar to those of large-conductance Ca(2+)-activated K(+) channels... - A novel kinetic assay of mitochondrial ATP-ADP exchange rate mediated by the ANTChristos Chinopoulos
Department of Medical Biochemistry, Semmelweis University, Neurobiochemical Group, Hungarian Academy of Sciences, Szentagothai Knowledge Center, Budapest, Hungary
Biophys J 96:2490-504. 2009..Finally, by comparing the ATP-ADP steady-state exchange rate to the amount of the ANT in rat brain synaptic, brain nonsynaptic, heart and liver mitochondria, we provide molecular turnover numbers for the known ANT isotypes... - Identification of direct and indirect effectors of the transient receptor potential melastatin 2 (TRPM2) cation channelBalazs Toth
Department of Medical Biochemistry, Semmelweis University, Budapest H 1094, Hungary
J Biol Chem 285:30091-102. 2010..H(2)O(2), cADPR, and NAADP did not enhance activation by ADPR. Considering intracellular concentrations of these compounds, none of them are likely to directly affect the TRPM2 channel protein in a physiological context... - Statistical evaluation of ion-channel gating models based on distributions of log-likelihood ratiosLASZLO CSANADY
Department of Medical Biochemistry, Semmelweis University, and Neurochemical Group of the Hungarian Academy of Sciences, Budapest, Hungary
Biophys J 90:3523-45. 2006..These observations are explained by the distributions of DeltaLL when Xi or Xi(R) is true... - Mitoxantrone is expelled by the ABCG2 multidrug transporter directly from the plasma membraneLaszlo Homolya
Membrane Biology Research Group, Semmelweis University, Hungarian Academy of Sciences, Diószegi u 64, H 1113 Budapest, Hungary
Biochim Biophys Acta 1808:154-63. 2011..On the basis of the kinetic analysis, drug extrusion from the cytoplasm can be excluded, thus, our results indicate that ABCG2 extrudes mitoxantrone directly from the plasma membrane...