Maria A Deli

Summary

Affiliation: Biological Research Centre
Location: Szeged, Hungary
URL: http://www.szbk.u-szeged.hu/biophys_blood_brain_barrier_pathology_and_pharmacology.php
Summary:
blood-brain barrier pathology and pharmacology
drug targeting to brain
nasal delivery
cell culture models
toxicity tests11117517

Publications

  1. doi Oxygen-glucose deprivation increases the enzymatic activity and the microvesicle-mediated release of ectonucleotidases in the cells composing the blood-brain barrier
    Stefania Ceruti
    Laboratory of Molecular and Cellular Pharmacology of Purinergic Transmission, Department of Pharmacological Sciences, Universita degli Studi di Milano, Via Balzaretti, 9, 20133 Milan, Italy
    Neurochem Int 59:259-71. 2011
  2. ncbi Pericytes from brain microvessels strengthen the barrier integrity in primary cultures of rat brain endothelial cells
    Shinsuke Nakagawa
    Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1 12 4 Sakamoto, Nagasaki 852 8523, Japan
    Cell Mol Neurobiol 27:687-94. 2007
  3. ncbi Highly activated c-fos expression in specific brain regions (ependyma, circumventricular organs, choroid plexus) of histidine decarboxylase deficient mice in response to formalin-induced acute pain
    Miklos Palkovits
    Neuromorphological and Neuroendocrine Research Laboratory of the Hungarian Academy of Sciences, Department of Anatomy, Semmelweis University, Budapest, Hungary
    Neuropharmacology 53:101-12. 2007
  4. ncbi The NMDA and AMPA/KA receptors are involved in glutamate-induced alterations of occludin expression and phosphorylation in brain endothelial cells
    Ibolya E Andras
    Molecular Neuroscience and Vascular Biology Laboratory, Department of Surgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
    J Cereb Blood Flow Metab 27:1431-43. 2007
  5. ncbi Pentosan polysulfate protects brain endothelial cells against bacterial lipopolysaccharide-induced damages
    Szilvia Veszelka
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
    Neurochem Int 50:219-28. 2007
  6. ncbi Permeability studies on in vitro blood-brain barrier models: physiology, pathology, and pharmacology
    Máiria A Deli
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari korut 62, H 6726 Szeged, Hungary
    Cell Mol Neurobiol 25:59-127. 2005
  7. ncbi Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific properties
    N Perriere
    CNRS UMR 7157, INSERM U705, University Paris 7, University Paris 5, Hopital Fernand Widal, Paris, France
    J Neurochem 93:279-89. 2005
  8. ncbi Signaling mechanisms of HIV-1 Tat-induced alterations of claudin-5 expression in brain endothelial cells
    Ibolya E Andras
    Department of Surgery, University of Kentucky, Lexington, Kentucky 40536, USA
    J Cereb Blood Flow Metab 25:1159-70. 2005
  9. ncbi Chronic adrenomedullin treatment improves blood-brain barrier function but has no effects on expression of tight junction proteins
    B Kis
    Department of Physiology and Pharmacology, Wake Forest University, School of Medicine, Winston Salem, NC 27157, USA
    Acta Neurochir Suppl 86:565-8. 2003
  10. ncbi Human serum amyloid P component attenuates the bacterial lipopolysaccharide-induced increase in blood-brain barrier permeability in mice
    Szilvia Veszelka
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
    Neurosci Lett 352:57-60. 2003

Collaborators

Detail Information

Publications59

  1. doi Oxygen-glucose deprivation increases the enzymatic activity and the microvesicle-mediated release of ectonucleotidases in the cells composing the blood-brain barrier
    Stefania Ceruti
    Laboratory of Molecular and Cellular Pharmacology of Purinergic Transmission, Department of Pharmacological Sciences, Universita degli Studi di Milano, Via Balzaretti, 9, 20133 Milan, Italy
    Neurochem Int 59:259-71. 2011
    ..This in vitro model is therefore useful to study the role of extracellular nucleotides in modulating BBB responses to ischemic events, and to develop new effective purinergic-based approaches for brain ischemia...
  2. ncbi Pericytes from brain microvessels strengthen the barrier integrity in primary cultures of rat brain endothelial cells
    Shinsuke Nakagawa
    Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1 12 4 Sakamoto, Nagasaki 852 8523, Japan
    Cell Mol Neurobiol 27:687-94. 2007
    ..6) The influence of pericytes on the BBB properties of brain endothelial cells may be as important as that of astrocytes and could be exploited in the construction of better BBB models...
  3. ncbi Highly activated c-fos expression in specific brain regions (ependyma, circumventricular organs, choroid plexus) of histidine decarboxylase deficient mice in response to formalin-induced acute pain
    Miklos Palkovits
    Neuromorphological and Neuroendocrine Research Laboratory of the Hungarian Academy of Sciences, Department of Anatomy, Semmelweis University, Budapest, Hungary
    Neuropharmacology 53:101-12. 2007
    ....
  4. ncbi The NMDA and AMPA/KA receptors are involved in glutamate-induced alterations of occludin expression and phosphorylation in brain endothelial cells
    Ibolya E Andras
    Molecular Neuroscience and Vascular Biology Laboratory, Department of Surgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
    J Cereb Blood Flow Metab 27:1431-43. 2007
    ..These findings indicate the opposite effects of the NMDA and AMPA/KA receptors on occludin phosphorylation and disruption of the BBB functions...
  5. ncbi Pentosan polysulfate protects brain endothelial cells against bacterial lipopolysaccharide-induced damages
    Szilvia Veszelka
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
    Neurochem Int 50:219-28. 2007
    ..The protective effect of pentosan for brain endothelium can be of therapeutical significance in bacterial infections affecting the BBB...
  6. ncbi Permeability studies on in vitro blood-brain barrier models: physiology, pathology, and pharmacology
    Máiria A Deli
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari korut 62, H 6726 Szeged, Hungary
    Cell Mol Neurobiol 25:59-127. 2005
    ....
  7. ncbi Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific properties
    N Perriere
    CNRS UMR 7157, INSERM U705, University Paris 7, University Paris 5, Hopital Fernand Widal, Paris, France
    J Neurochem 93:279-89. 2005
    ..In conclusion, this method of RBEC purification will allow the production of in vitro models of the rat blood-brain barrier for cellular and molecular biology studies as well as pharmacological investigations...
  8. ncbi Signaling mechanisms of HIV-1 Tat-induced alterations of claudin-5 expression in brain endothelial cells
    Ibolya E Andras
    Department of Surgery, University of Kentucky, Lexington, Kentucky 40536, USA
    J Cereb Blood Flow Metab 25:1159-70. 2005
    ..Because claudins constitute the major backbone of tight junctions, the present data are relevant to the disturbances of the BBB in the course of HIV-1 infection...
  9. ncbi Chronic adrenomedullin treatment improves blood-brain barrier function but has no effects on expression of tight junction proteins
    B Kis
    Department of Physiology and Pharmacology, Wake Forest University, School of Medicine, Winston Salem, NC 27157, USA
    Acta Neurochir Suppl 86:565-8. 2003
    ....
  10. ncbi Human serum amyloid P component attenuates the bacterial lipopolysaccharide-induced increase in blood-brain barrier permeability in mice
    Szilvia Veszelka
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
    Neurosci Lett 352:57-60. 2003
    ..Our data indicate that human SAP may counteract the toxic effects of LPS during septic shock...
  11. ncbi HIV-1 Tat protein alters tight junction protein expression and distribution in cultured brain endothelial cells
    Ibolya E Andras
    Department of Surgery, University of Kentucky, Lexington, Kentucky, USA
    J Neurosci Res 74:255-65. 2003
    ..Because tight junction proteins are critical for the barrier function of the BBB, such alterations can lead to disturbances of the BBB integrity and contribute to HIV trafficking into the brain...
  12. ncbi Signalling pathways regulating the tight junction permeability in the blood-brain barrier
    I A Krizbai
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, P O Box 521, H 6701 Szeged, Hungary
    Cell Mol Biol (Noisy-le-grand) 49:23-31. 2003
    ....
  13. ncbi Vascular changes play a role in the pathogenesis of necrotizing enterocolitis in asphyxiated newborn pigs
    B Gellen
    Department of Paediatrics, University of Szeged, Koranyi fasor 14 15, 6720 Szeged, Hungary
    Pediatr Surg Int 19:380-4. 2003
    ..In conclusion, severe vasomotor changes during asphyxia may result in mesenteric endothelial dysfunction implicated in increased vascular permeability, edema formation, and development of NEC in asphyxiated piglets...
  14. ncbi Effect of nicotine and polyaromtic hydrocarbons on cerebral endothelial cells
    Pilaiwanwadee Hutamekalin
    Institute of Biophysics, Biological Research Centre, Temesvari krt 62, 6726 Szeged, Hungary
    Cell Biol Int 32:198-209. 2008
    ..Our experiments suggest that tobacco smoking may affect the junctional complex of CECs, and that this effect is enhanced by oxidative stress...
  15. doi Epithelial and endothelial barriers in the olfactory region of the nasal cavity of the rat
    Hartwig Wolburg
    Institute of Pathology, University of Tubingen, Liebermeisterstrasse 8, 72076, Tubingen, Germany
    Histochem Cell Biol 130:127-40. 2008
    ..The tight junctions of the OECs are discussed to be responsible for micro-compartmentalization within the olfactory fiber tract providing a benefit for axonal growth...
  16. doi Potential use of tight junction modulators to reversibly open membranous barriers and improve drug delivery
    Maria A Deli
    Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
    Biochim Biophys Acta 1788:892-910. 2009
    ....
  17. doi Randomly methylated β-cyclodextrin derivatives enhance taxol permeability through human intestinal epithelial Caco-2 cell monolayer
    Ferenc Fenyvesi
    Department of Pharmaceutical Technology, Faculty of Pharmacy, Medical and Health Science Center, University of Debrecen, H 4010 Debrecen, Hungary
    J Pharm Sci 100:4734-44. 2011
    ..In conclusion, second-generation derivatives of methyl-β-cyclodextrin, especially MaRAMEB, enhanced taxol permeability across Caco-2 cells with less toxicity and similar effectiveness as RAMEB...
  18. ncbi Patented in vitro blood-brain barrier models in CNS drug discovery
    Andrea Tóth
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary
    Recent Pat CNS Drug Discov 6:107-18. 2011
    ..Though no "ideal in vitro BBB model" has been constructed yet, the currently available models provide valuable information on BBB permeability and are useful tools in CNS drug discovery...
  19. doi Glial cells drive preconditioning-induced blood-brain barrier protection
    Raffaella Gesuete
    Laboratory of Inflammation and Nervous System Diseases, Mario Negri Institute, Via G La Masa 19, 20156 Milan, Italy
    Stroke 42:1445-53. 2011
    ..The cerebrovascular contribution to ischemic preconditioning (IPC) has been scarcely explored. Using in vivo and in vitro approaches, we investigated the involvement of the blood-brain barrier and the role of its cellular components...
  20. doi Applicability of sucrose laurate as surfactant in solid dispersions prepared by melt technology
    Angéla Szuts
    Department of Pharmaceutical Technology, University of Szeged, H 6720 Szeged, Eotvos u 6, Hungary
    Int J Pharm 410:107-10. 2011
    ..The effective final concentrations of D1216 in the dissolution experiments proved to be non-toxic towards CaCo-2 cells. The results suggest the potential use of D1216 in innovative internal pharmaceutical formulations...
  21. ncbi Intracellular transport of Toxoplasma gondii through the blood-brain barrier
    Sabrina M Lachenmaier
    Institute of Microbiology and Hygiene, Charite Medical School Berlin, Campus Benjamin Franklin, Berlin, Germany
    J Neuroimmunol 232:119-30. 2011
    ..gondii across the BBB. T. gondii type I and type II strains induced similar migration patterns of antigen-presenting cells across the in vitro BBB...
  22. doi Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters
    Bence György
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Blood 117:e39-48. 2011
    ..These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical laboratory assessment of the presence and biologic functions of MPs in health and disease...
  23. doi Protection of the blood-brain barrier by pentosan against amyloid-β-induced toxicity
    Maria A Deli
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
    J Alzheimers Dis 22:777-94. 2010
    ..The present data further support the toxic effects of amyloid peptides on brain endothelial cells, and can contribute to the development of molecules protecting the blood-brain barrier in Alzheimer's disease...
  24. ncbi Pitavastatin strengthens the barrier integrity in primary cultures of rat brain endothelial cells
    Yoichi Morofuji
    Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1 7 1 Sakamoto, Nagasaki 852 8501, Japan
    Cell Mol Neurobiol 30:727-35. 2010
    ..The BBB-stabilizing effect of pitavastatin may be mediated partly through inhibition of the mevalonate pathway and subsequent up-regulation of claudin-5 expression...
  25. doi Intranasal delivery of human beta-amyloid peptide in rats: effective brain targeting
    Eszter Sipos
    Department of Medical Chemistry, University of Szeged, Szeged, Hungary
    Cell Mol Neurobiol 30:405-13. 2010
    ....
  26. doi Human truncated tau is using a different mechanism from amyloid-beta to damage the blood-brain barrier
    Andrej Kovac
    Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovak Republic
    J Alzheimers Dis 18:897-906. 2009
    ..This study for the first time uncovers a novel toxic gain of function of misfolded tau that could contribute to the cerebral microvascular damage in human tauopathies...
  27. ncbi A new blood-brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes
    Shinsuke Nakagawa
    Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto, Japan
    Neurochem Int 54:253-63. 2009
    ..The new BBB model, which is the first model to incorporate pericytes in a triple co-culture setting, can be a useful tool for research on BBB physiology and pathology and to test candidate compounds for centrally acting drugs...
  28. doi Sodium hyaluronate as a mucoadhesive component in nasal formulation enhances delivery of molecules to brain tissue
    Sándor Horvát
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmaceutical Technology, University of Szeged, Szeged, Hungary
    Eur J Pharm Biopharm 72:252-9. 2009
    ..Hyaluronan, a non-toxic biomolecule used as a mucoadhesive in a nasal formulation, increased the brain penetration of a hydrophilic compound, the size of a peptide, via the nasal route...
  29. ncbi N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine increases the permeability of primary mouse cerebral endothelial cell monolayers
    M A Deli
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy, Temesvari korut 62, H 6726 Szeged, Hungary
    Inflamm Res 52:S39-40. 2003
  30. ncbi Adrenomedullin, an autocrine mediator of blood-brain barrier function
    Bela Kis
    Department of Physiology and Pharmacology, Wake Forest University, Medical Center Boulevard, Winston Salem, NC 27157, USA
    Hypertens Res 26:S61-70. 2003
    ....
  31. ncbi Transient forebrain ischemia increases the blood-brain barrier permeability for albumin in stroke-prone spontaneously hypertensive rats
    Csongor S Abrahám
    Department of Pharmacology 1, Nagasaki University School of Medicine, 1 12 4 Sakamoto, Nagasaki 852 8523, Japan
    Cell Mol Neurobiol 22:455-62. 2002
    ..4. Extravasation of serum macromolecules may contribute to neuronal loss and development of hypertensive encephalopathy in SHRSP...
  32. ncbi Experimental acute pancreatitis results in increased blood-brain barrier permeability in the rat: a potential role for tumor necrosis factor and interleukin 6
    G Farkas
    Department of Surgery, Albert Szent Gyorgyi University Medical School, Szeged, Hungary
    Neurosci Lett 242:147-50. 1998
    ..We conclude that cytokines, such as TNF and IL-6, may contribute to the vasogenic brain edema formation during acute pancreatitis...
  33. ncbi Intracarotid tumor necrosis factor-alpha administration increases the blood-brain barrier permeability in cerebral cortex of the newborn pig: quantitative aspects of double-labelling studies and confocal laser scanning analysis
    C S Abraham
    Department of Pediatrics, Albert Szent Gyorgyi Medical University, Szeged, Hungary
    Neurosci Lett 208:85-8. 1996
    ..We conclude that significant increases in intravascular TNF-alpha-concentration during neonatal infections may result in vasogenic brain edema formation...
  34. ncbi Expression of glutamate receptors on cultured cerebral endothelial cells
    I A Krizbai
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged
    J Neurosci Res 54:814-9. 1998
    ..This effect could be prevented by the NMDA blocker MK-801. The presence of multiple glutamate receptor types may confer a finely tuned responsiveness of the cerebral endothelium to glutamate in physiological and pathological conditions...
  35. ncbi Adrenomedullin and migraine
    Kis Béla
    Headache 42:1064-5. 2002
  36. ncbi Cerebral endothelial cells are a major source of adrenomedullin
    B Kis
    Department of Physiology, University of Occupational and Environmental Health, Kitakyushu, Japan
    J Neuroendocrinol 14:283-93. 2002
    ..Cerebral endothelial cells are a potential source of adrenomedullin in the central nervous system, where adrenomedullin can also be involved in the regulation of neuroendocrine functions...
  37. ncbi Adrenomedullin in the cerebral circulation
    B Kis
    Department of Physiology, University of Occupational and Environmental Health, 807 8555, Kitakyushu, Japan
    Peptides 22:1825-34. 2001
    ..In this review, we describe current knowledge about the origin and possible role of adrenomedullin in the regulation of cerebral circulation and blood-brain barrier functions...
  38. ncbi Adrenomedullin regulates blood-brain barrier functions in vitro
    B Kis
    Department of Physiology, University of Occupational and Environmental Health, Iseigaoka 1-1 Yahatanishi-ku, Kitakyushu 807-8555, Japan
    Neuroreport 12:4139-42. 2001
    ..AM treatment resulted in functional activation of P-glycoprotein efflux pump in vitro. Our results indicate that AM as an autocrine mediator plays an important role in the regulation of BBB properties of the cerebral endothelial cells...
  39. ncbi Asphyxia-induced release of alpha-melanocyte-stimulating hormone in newborn pigs
    J Kovacs
    Department of Pediatrics, University of Szeged, Koranyi fasor 14 15, H 6720 Szeged, Hungary
    Peptides 22:1049-53. 2001
    ..05) reduced by 50% during postasphyxial reperfusion. Our data indicate an asphyxia-induced release of alpha-MSH, and suggest a discordant regulation of plasma and CSF concentrations in newborn pigs...
  40. ncbi Tissue plasminogen activator inhibits P-glycoprotein activity in brain endothelial cells
    M A Deli
    Department of Pharmacology 1, Nagasaki University School of Medicine, 1 12 4 Sakamoto, 852 8523, Nagasaki, Japan
    Eur J Pharmacol 411:R3-R5. 2001
    ..Elevation of intra-endothelial cyclic AMP concentration and stimulation of protein kinase C increased P-glycoprotein activity in GP8 cells and also attenuated the tissue plasminogen activator-induced inhibition...
  41. ncbi Pentosan polysulfate regulates scavenger receptor-mediated, but not fluid-phase, endocytosis in immortalized cerebral endothelial cells
    M A Deli
    Department of Pharmacology 1, Nagasaki University School of Medicine, Japan
    Cell Mol Neurobiol 20:731-45. 2000
    ..5. In conclusion. PPS decreased the binding and increased the uptake of DiI-AcLDL in cerebral endothelial cells, an effect not mimicked by the other polyanions investigated...
  42. ncbi Effects of N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine on the blood-brain barrier permeability in the rat
    M A Deli
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari korut 62, H 6701, Szeged, Hungary
    Eur J Pharmacol 387:63-72. 2000
    ..In conclusion, DPPE induced an increased permeability in the rat, which supports a role for histamine, as an intracellular messenger, in the regulation of the blood-brain barrier characteristics...
  43. ncbi 4-(2-Aminoethyl)benzenesulfonyl fluoride attenuates tumor-necrosis-factor-alpha-induced blood-brain barrier opening
    P Megyeri
    Department of Pediatrics, Albert Szent Gyorgyi Medical University, Szeged, Hungary
    Eur J Pharmacol 374:207-11. 1999
    ..2 mg/kg AEBSF could significantly (P < 0.05) decrease the change in Evan's blue-albumin (MW = 67,000) transport in two regions. In conclusion, AEBSF attenuates vasogenic brain edema formation...
  44. ncbi Vasoactive substances produced by cultured rat brain endothelial cells
    B Kis
    Department of Pathophysiology, Albert Szent Gyorgyi Medical University, Szeged, Hungary
    Eur J Pharmacol 368:35-42. 1999
    ..The capacity of cerebral endothelium to regulate vascular tone by production of dilator and constrictor substances can be preserved under certain circumstances in immortalized cell lines...