Genomes and Genes
Maria A Deli
Affiliation: Biological Research Centre
Location: Szeged, Hungary
blood-brain barrier pathology and pharmacology
drug targeting to brain
cell culture models
- Oxygen-glucose deprivation increases the enzymatic activity and the microvesicle-mediated release of ectonucleotidases in the cells composing the blood-brain barrierStefania Ceruti
Laboratory of Molecular and Cellular Pharmacology of Purinergic Transmission, Department of Pharmacological Sciences, Universita degli Studi di Milano, Via Balzaretti, 9, 20133 Milan, Italy
Neurochem Int 59:259-71. 2011..This in vitro model is therefore useful to study the role of extracellular nucleotides in modulating BBB responses to ischemic events, and to develop new effective purinergic-based approaches for brain ischemia...
- Pericytes from brain microvessels strengthen the barrier integrity in primary cultures of rat brain endothelial cellsShinsuke Nakagawa
Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1 12 4 Sakamoto, Nagasaki 852 8523, Japan
Cell Mol Neurobiol 27:687-94. 2007..6) The influence of pericytes on the BBB properties of brain endothelial cells may be as important as that of astrocytes and could be exploited in the construction of better BBB models...
- Highly activated c-fos expression in specific brain regions (ependyma, circumventricular organs, choroid plexus) of histidine decarboxylase deficient mice in response to formalin-induced acute painMiklos Palkovits
Neuromorphological and Neuroendocrine Research Laboratory of the Hungarian Academy of Sciences, Department of Anatomy, Semmelweis University, Budapest, Hungary
Neuropharmacology 53:101-12. 2007....
- The NMDA and AMPA/KA receptors are involved in glutamate-induced alterations of occludin expression and phosphorylation in brain endothelial cellsIbolya E Andras
Molecular Neuroscience and Vascular Biology Laboratory, Department of Surgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA
J Cereb Blood Flow Metab 27:1431-43. 2007..These findings indicate the opposite effects of the NMDA and AMPA/KA receptors on occludin phosphorylation and disruption of the BBB functions...
- Pentosan polysulfate protects brain endothelial cells against bacterial lipopolysaccharide-induced damagesSzilvia Veszelka
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
Neurochem Int 50:219-28. 2007..The protective effect of pentosan for brain endothelium can be of therapeutical significance in bacterial infections affecting the BBB...
- Permeability studies on in vitro blood-brain barrier models: physiology, pathology, and pharmacologyMáiria A Deli
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari korut 62, H 6726 Szeged, Hungary
Cell Mol Neurobiol 25:59-127. 2005....
- Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific propertiesN Perriere
CNRS UMR 7157, INSERM U705, University Paris 7, University Paris 5, Hopital Fernand Widal, Paris, France
J Neurochem 93:279-89. 2005..In conclusion, this method of RBEC purification will allow the production of in vitro models of the rat blood-brain barrier for cellular and molecular biology studies as well as pharmacological investigations...
- Signaling mechanisms of HIV-1 Tat-induced alterations of claudin-5 expression in brain endothelial cellsIbolya E Andras
Department of Surgery, University of Kentucky, Lexington, Kentucky 40536, USA
J Cereb Blood Flow Metab 25:1159-70. 2005..Because claudins constitute the major backbone of tight junctions, the present data are relevant to the disturbances of the BBB in the course of HIV-1 infection...
- Chronic adrenomedullin treatment improves blood-brain barrier function but has no effects on expression of tight junction proteinsB Kis
Department of Physiology and Pharmacology, Wake Forest University, School of Medicine, Winston Salem, NC 27157, USA
Acta Neurochir Suppl 86:565-8. 2003....
- Human serum amyloid P component attenuates the bacterial lipopolysaccharide-induced increase in blood-brain barrier permeability in miceSzilvia Veszelka
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, , H-6726 Szeged, Hungary
Neurosci Lett 352:57-60. 2003..Our data indicate that human SAP may counteract the toxic effects of LPS during septic shock...
- HIV-1 Tat protein alters tight junction protein expression and distribution in cultured brain endothelial cellsIbolya E Andras
Department of Surgery, University of Kentucky, Lexington, Kentucky, USA
J Neurosci Res 74:255-65. 2003..Because tight junction proteins are critical for the barrier function of the BBB, such alterations can lead to disturbances of the BBB integrity and contribute to HIV trafficking into the brain...
- Signalling pathways regulating the tight junction permeability in the blood-brain barrierI A Krizbai
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, P O Box 521, H 6701 Szeged, Hungary
Cell Mol Biol (Noisy-le-grand) 49:23-31. 2003....
- Vascular changes play a role in the pathogenesis of necrotizing enterocolitis in asphyxiated newborn pigsB Gellen
Department of Paediatrics, University of Szeged, Koranyi fasor 14 15, 6720 Szeged, Hungary
Pediatr Surg Int 19:380-4. 2003..In conclusion, severe vasomotor changes during asphyxia may result in mesenteric endothelial dysfunction implicated in increased vascular permeability, edema formation, and development of NEC in asphyxiated piglets...
- Effect of nicotine and polyaromtic hydrocarbons on cerebral endothelial cellsPilaiwanwadee Hutamekalin
Institute of Biophysics, Biological Research Centre, Temesvari krt 62, 6726 Szeged, Hungary
Cell Biol Int 32:198-209. 2008..Our experiments suggest that tobacco smoking may affect the junctional complex of CECs, and that this effect is enhanced by oxidative stress...
- Epithelial and endothelial barriers in the olfactory region of the nasal cavity of the ratHartwig Wolburg
Institute of Pathology, University of Tubingen, Liebermeisterstrasse 8, 72076, Tubingen, Germany
Histochem Cell Biol 130:127-40. 2008..The tight junctions of the OECs are discussed to be responsible for micro-compartmentalization within the olfactory fiber tract providing a benefit for axonal growth...
- Potential use of tight junction modulators to reversibly open membranous barriers and improve drug deliveryMaria A Deli
Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt 62, H 6726 Szeged, Hungary
Biochim Biophys Acta 1788:892-910. 2009....
- Randomly methylated β-cyclodextrin derivatives enhance taxol permeability through human intestinal epithelial Caco-2 cell monolayerFerenc Fenyvesi
Department of Pharmaceutical Technology, Faculty of Pharmacy, Medical and Health Science Center, University of Debrecen, H 4010 Debrecen, Hungary
J Pharm Sci 100:4734-44. 2011..In conclusion, second-generation derivatives of methyl-β-cyclodextrin, especially MaRAMEB, enhanced taxol permeability across Caco-2 cells with less toxicity and similar effectiveness as RAMEB...
- Patented in vitro blood-brain barrier models in CNS drug discoveryAndrea Tóth
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary
Recent Pat CNS Drug Discov 6:107-18. 2011..Though no "ideal in vitro BBB model" has been constructed yet, the currently available models provide valuable information on BBB permeability and are useful tools in CNS drug discovery...
- Glial cells drive preconditioning-induced blood-brain barrier protectionRaffaella Gesuete
Laboratory of Inflammation and Nervous System Diseases, Mario Negri Institute, Via G La Masa 19, 20156 Milan, Italy
Stroke 42:1445-53. 2011..The cerebrovascular contribution to ischemic preconditioning (IPC) has been scarcely explored. Using in vivo and in vitro approaches, we investigated the involvement of the blood-brain barrier and the role of its cellular components...
- Applicability of sucrose laurate as surfactant in solid dispersions prepared by melt technologyAngéla Szuts
Department of Pharmaceutical Technology, University of Szeged, H 6720 Szeged, Eotvos u 6, Hungary
Int J Pharm 410:107-10. 2011..The effective final concentrations of D1216 in the dissolution experiments proved to be non-toxic towards CaCo-2 cells. The results suggest the potential use of D1216 in innovative internal pharmaceutical formulations...
- Intracellular transport of Toxoplasma gondii through the blood-brain barrierSabrina M Lachenmaier
Institute of Microbiology and Hygiene, Charite Medical School Berlin, Campus Benjamin Franklin, Berlin, Germany
J Neuroimmunol 232:119-30. 2011..gondii across the BBB. T. gondii type I and type II strains induced similar migration patterns of antigen-presenting cells across the in vitro BBB...
- Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parametersBence György
Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
Blood 117:e39-48. 2011..These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical laboratory assessment of the presence and biologic functions of MPs in health and disease...
- Protection of the blood-brain barrier by pentosan against amyloid-β-induced toxicityMaria A Deli
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
J Alzheimers Dis 22:777-94. 2010..The present data further support the toxic effects of amyloid peptides on brain endothelial cells, and can contribute to the development of molecules protecting the blood-brain barrier in Alzheimer's disease...
- Pitavastatin strengthens the barrier integrity in primary cultures of rat brain endothelial cellsYoichi Morofuji
Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1 7 1 Sakamoto, Nagasaki 852 8501, Japan
Cell Mol Neurobiol 30:727-35. 2010..The BBB-stabilizing effect of pitavastatin may be mediated partly through inhibition of the mevalonate pathway and subsequent up-regulation of claudin-5 expression...
- Intranasal delivery of human beta-amyloid peptide in rats: effective brain targetingEszter Sipos
Department of Medical Chemistry, University of Szeged, Szeged, Hungary
Cell Mol Neurobiol 30:405-13. 2010....
- Human truncated tau is using a different mechanism from amyloid-beta to damage the blood-brain barrierAndrej Kovac
Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovak Republic
J Alzheimers Dis 18:897-906. 2009..This study for the first time uncovers a novel toxic gain of function of misfolded tau that could contribute to the cerebral microvascular damage in human tauopathies...
- A new blood-brain barrier model using primary rat brain endothelial cells, pericytes and astrocytesShinsuke Nakagawa
Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto, Japan
Neurochem Int 54:253-63. 2009..The new BBB model, which is the first model to incorporate pericytes in a triple co-culture setting, can be a useful tool for research on BBB physiology and pathology and to test candidate compounds for centrally acting drugs...
- Sodium hyaluronate as a mucoadhesive component in nasal formulation enhances delivery of molecules to brain tissueSándor Horvát
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmaceutical Technology, University of Szeged, Szeged, Hungary
Eur J Pharm Biopharm 72:252-9. 2009..Hyaluronan, a non-toxic biomolecule used as a mucoadhesive in a nasal formulation, increased the brain penetration of a hydrophilic compound, the size of a peptide, via the nasal route...
- N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine increases the permeability of primary mouse cerebral endothelial cell monolayersM A Deli
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy, , H-6726 Szeged, Hungary
Inflamm Res 52:S39-40. 2003
- Adrenomedullin, an autocrine mediator of blood-brain barrier functionBela Kis
Department of Physiology and Pharmacology, Wake Forest University, Medical Center Boulevard, Winston Salem, NC 27157, USA
Hypertens Res 26:S61-70. 2003....
- Transient forebrain ischemia increases the blood-brain barrier permeability for albumin in stroke-prone spontaneously hypertensive ratsCsongor S Abrahám
Department of Pharmacology 1, Nagasaki University School of Medicine, 1 12 4 Sakamoto, Nagasaki 852 8523, Japan
Cell Mol Neurobiol 22:455-62. 2002..4. Extravasation of serum macromolecules may contribute to neuronal loss and development of hypertensive encephalopathy in SHRSP...
- Experimental acute pancreatitis results in increased blood-brain barrier permeability in the rat: a potential role for tumor necrosis factor and interleukin 6G Farkas
Department of Surgery, Albert Szent Gyorgyi University Medical School, Szeged, Hungary
Neurosci Lett 242:147-50. 1998..We conclude that cytokines, such as TNF and IL-6, may contribute to the vasogenic brain edema formation during acute pancreatitis...
- Intracarotid tumor necrosis factor-alpha administration increases the blood-brain barrier permeability in cerebral cortex of the newborn pig: quantitative aspects of double-labelling studies and confocal laser scanning analysisC S Abraham
Department of Pediatrics, Albert Szent Gyorgyi Medical University, Szeged, Hungary
Neurosci Lett 208:85-8. 1996..We conclude that significant increases in intravascular TNF-alpha-concentration during neonatal infections may result in vasogenic brain edema formation...
- Expression of glutamate receptors on cultured cerebral endothelial cellsI A Krizbai
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, Szeged
J Neurosci Res 54:814-9. 1998..This effect could be prevented by the NMDA blocker MK-801. The presence of multiple glutamate receptor types may confer a finely tuned responsiveness of the cerebral endothelium to glutamate in physiological and pathological conditions...
- Adrenomedullin and migraineKis Béla
Headache 42:1064-5. 2002
- Cerebral endothelial cells are a major source of adrenomedullinB Kis
Department of Physiology, University of Occupational and Environmental Health, Kitakyushu, Japan
J Neuroendocrinol 14:283-93. 2002..Cerebral endothelial cells are a potential source of adrenomedullin in the central nervous system, where adrenomedullin can also be involved in the regulation of neuroendocrine functions...
- Adrenomedullin in the cerebral circulationB Kis
Department of Physiology, University of Occupational and Environmental Health, 807 8555, Kitakyushu, Japan
Peptides 22:1825-34. 2001..In this review, we describe current knowledge about the origin and possible role of adrenomedullin in the regulation of cerebral circulation and blood-brain barrier functions...
- Adrenomedullin regulates blood-brain barrier functions in vitroB Kis
Department of Physiology, University of Occupational and Environmental Health, Iseigaoka 1-1 Yahatanishi-ku, Kitakyushu 807-8555, Japan
Neuroreport 12:4139-42. 2001..AM treatment resulted in functional activation of P-glycoprotein efflux pump in vitro. Our results indicate that AM as an autocrine mediator plays an important role in the regulation of BBB properties of the cerebral endothelial cells...
- Asphyxia-induced release of alpha-melanocyte-stimulating hormone in newborn pigsJ Kovacs
Department of Pediatrics, University of Szeged, Koranyi fasor 14 15, H 6720 Szeged, Hungary
Peptides 22:1049-53. 2001..05) reduced by 50% during postasphyxial reperfusion. Our data indicate an asphyxia-induced release of alpha-MSH, and suggest a discordant regulation of plasma and CSF concentrations in newborn pigs...
- Tissue plasminogen activator inhibits P-glycoprotein activity in brain endothelial cellsM A Deli
Department of Pharmacology 1, Nagasaki University School of Medicine, 1 12 4 Sakamoto, 852 8523, Nagasaki, Japan
Eur J Pharmacol 411:R3-R5. 2001..Elevation of intra-endothelial cyclic AMP concentration and stimulation of protein kinase C increased P-glycoprotein activity in GP8 cells and also attenuated the tissue plasminogen activator-induced inhibition...
- Pentosan polysulfate regulates scavenger receptor-mediated, but not fluid-phase, endocytosis in immortalized cerebral endothelial cellsM A Deli
Department of Pharmacology 1, Nagasaki University School of Medicine, Japan
Cell Mol Neurobiol 20:731-45. 2000..5. In conclusion. PPS decreased the binding and increased the uptake of DiI-AcLDL in cerebral endothelial cells, an effect not mimicked by the other polyanions investigated...
- Effects of N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine on the blood-brain barrier permeability in the ratM A Deli
Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari korut 62, H 6701, Szeged, Hungary
Eur J Pharmacol 387:63-72. 2000..In conclusion, DPPE induced an increased permeability in the rat, which supports a role for histamine, as an intracellular messenger, in the regulation of the blood-brain barrier characteristics...
- 4-(2-Aminoethyl)benzenesulfonyl fluoride attenuates tumor-necrosis-factor-alpha-induced blood-brain barrier openingP Megyeri
Department of Pediatrics, Albert Szent Gyorgyi Medical University, Szeged, Hungary
Eur J Pharmacol 374:207-11. 1999..2 mg/kg AEBSF could significantly (P < 0.05) decrease the change in Evan's blue-albumin (MW = 67,000) transport in two regions. In conclusion, AEBSF attenuates vasogenic brain edema formation...
- Vasoactive substances produced by cultured rat brain endothelial cellsB Kis
Department of Pathophysiology, Albert Szent Gyorgyi Medical University, Szeged, Hungary
Eur J Pharmacol 368:35-42. 1999..The capacity of cerebral endothelium to regulate vascular tone by production of dilator and constrictor substances can be preserved under certain circumstances in immortalized cell lines...