Stephen L Hoffman

Summary

Publications

  1. pmc Controlled human malaria infections by intradermal injection of cryopreserved Plasmodium falciparum sporozoites
    Meta Roestenberg
    Radboud University Nijmegen Medical Center, Department of Medical Microbiology, Nijmegen, The Netherlands
    Am J Trop Med Hyg 88:5-13. 2013
  2. pmc Establishment of an in vitro assay for assessing the effects of drugs on the liver stages of Plasmodium vivax malaria
    Rana Chattopadhyay
    Sanaria Inc, Rockville, Maryland, United States of America
    PLoS ONE 5:e14275. 2010
  3. pmc Plasmodium yoelii-infected A. stephensi inefficiently transmit malaria compared to intravenous route
    Solomon Conteh
    Sanaria Inc, Rockville, Maryland, United States of America
    PLoS ONE 5:e8947. 2010
  4. pmc Cross-protection between attenuated Plasmodium berghei and P. yoelii sporozoites
    M Sedegah
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Parasite Immunol 29:559-65. 2007
  5. pmc Haemoglobin dynamics in Papuan and non-Papuan adults in northeast Papua, Indonesia, with acute, uncomplicated vivax or falciparum malaria
    Walter R J Taylor
    The US Navy Medical Research Unit no 2 NAMRU 2, Jakarta, Indonesia
    Malar J 12:209. 2013
  6. pmc Changes in the total leukocyte and platelet counts in Papuan and non Papuan adults from northeast Papua infected with acute Plasmodium vivax or uncomplicated Plasmodium falciparum malaria
    Walter R J Taylor
    The US Navy Medical Research Unit no 2, Jakarta, Indonesia
    Malar J 7:259. 2008
  7. pmc Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor
    Jonathan A Chappel
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Malar J 3:28. 2004
  8. ncbi request reprint Development of a metabolically active, non-replicating sporozoite vaccine to prevent Plasmodium falciparum malaria
    Stephen L Hoffman
    Sanaria Inc, Rockville, MD, USA
    Hum Vaccin 6:97-106. 2010
  9. doi request reprint Plasmodium: mammalian codon optimization of malaria plasmid DNA vaccines enhances antibody responses but not T cell responses nor protective immunity
    Carlota Dobano
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Exp Parasitol 122:112-23. 2009
  10. pmc Boosting of DNA vaccine-elicited gamma interferon responses in humans by exposure to malaria parasites
    Ruobing Wang
    Sanaria, Inc, 12115 Parklawn Drive, Suite L, Rockville, MD 20852, USA
    Infect Immun 73:2863-72. 2005

Research Grants

Detail Information

Publications50

  1. pmc Controlled human malaria infections by intradermal injection of cryopreserved Plasmodium falciparum sporozoites
    Meta Roestenberg
    Radboud University Nijmegen Medical Center, Department of Medical Microbiology, Nijmegen, The Netherlands
    Am J Trop Med Hyg 88:5-13. 2013
    ..After further optimization, the use of such PfSPZ may facilitate and accelerate clinical development of novel malaria drugs and vaccines...
  2. pmc Establishment of an in vitro assay for assessing the effects of drugs on the liver stages of Plasmodium vivax malaria
    Rana Chattopadhyay
    Sanaria Inc, Rockville, Maryland, United States of America
    PLoS ONE 5:e14275. 2010
    ..We have also identified small parasite forms that may be hypnozoites. These data provide the foundation for finalizing a medium throughput, high content assay to identify new drugs for the elimination of all Pv liver stages...
  3. pmc Plasmodium yoelii-infected A. stephensi inefficiently transmit malaria compared to intravenous route
    Solomon Conteh
    Sanaria Inc, Rockville, Maryland, United States of America
    PLoS ONE 5:e8947. 2010
    ....
  4. pmc Cross-protection between attenuated Plasmodium berghei and P. yoelii sporozoites
    M Sedegah
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Parasite Immunol 29:559-65. 2007
    ..berghei, but no protection against P. yoelii. The cross-protection data in BALB/c mice support testing a human vaccine based on attenuated PfSPZ for its efficacy against P. vivax...
  5. pmc Haemoglobin dynamics in Papuan and non-Papuan adults in northeast Papua, Indonesia, with acute, uncomplicated vivax or falciparum malaria
    Walter R J Taylor
    The US Navy Medical Research Unit no 2 NAMRU 2, Jakarta, Indonesia
    Malar J 12:209. 2013
    ..Haemoglobin (Hb) recovers slowly in malaria and may be influenced by naturally acquired immunity. Hb recovery was compared in malaria immune, indigenous Papuan and non-Papuan adults with limited malaria exposure...
  6. pmc Changes in the total leukocyte and platelet counts in Papuan and non Papuan adults from northeast Papua infected with acute Plasmodium vivax or uncomplicated Plasmodium falciparum malaria
    Walter R J Taylor
    The US Navy Medical Research Unit no 2, Jakarta, Indonesia
    Malar J 7:259. 2008
    ..There are limited data on the evolution of the leukocyte and platelet counts in malaria patients...
  7. pmc Molecular dissection of the human antibody response to the structural repeat epitope of Plasmodium falciparum sporozoite from a protected donor
    Jonathan A Chappel
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Malar J 3:28. 2004
    ..In individuals immunized by the bites of irradiated Anopheles mosquitoes carrying P. falciparum sporozoites in their salivary glands, the anti-repeat response dominates and is thought by many to play a role in protective immunity...
  8. ncbi request reprint Development of a metabolically active, non-replicating sporozoite vaccine to prevent Plasmodium falciparum malaria
    Stephen L Hoffman
    Sanaria Inc, Rockville, MD, USA
    Hum Vaccin 6:97-106. 2010
    ..A medium term goal is to design and build a facility for manufacturing highly potent and stable vaccine for pivotal Phase 3 studies and commercial launch...
  9. doi request reprint Plasmodium: mammalian codon optimization of malaria plasmid DNA vaccines enhances antibody responses but not T cell responses nor protective immunity
    Carlota Dobano
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Exp Parasitol 122:112-23. 2009
    ..These data demonstrate that the effect of mammalian codon optimization is antigen-dependent and may not be beneficial for vaccines designed to induce T cell dependent protective immunity in this malaria model...
  10. pmc Boosting of DNA vaccine-elicited gamma interferon responses in humans by exposure to malaria parasites
    Ruobing Wang
    Sanaria, Inc, 12115 Parklawn Drive, Suite L, Rockville, MD 20852, USA
    Infect Immun 73:2863-72. 2005
    ....
  11. ncbi request reprint Induction in humans of CD8+ and CD4+ T cell and antibody responses by sequential immunization with malaria DNA and recombinant protein
    Ruobing Wang
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    J Immunol 172:5561-9. 2004
    ..The induction in humans of the three primary Ag-specific adaptive immune responses establishes a strategy for developing immunization regimens against diseases in desperate need of vaccines...
  12. ncbi request reprint Successful induction of CD8 T cell-dependent protection against malaria by sequential immunization with DNA and recombinant poxvirus of neonatal mice born to immune mothers
    Martha Sedegah
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    J Immunol 171:3148-53. 2003
    ..yoelii parasites or immunized with the vaccine were protected and had excellent T cell responses. These data support assessment of this immunization strategy in neonates/young infants in areas in which malaria exacts its greatest toll...
  13. pmc Protection of rhesus macaques against lethal Plasmodium knowlesi malaria by a heterologous DNA priming and poxvirus boosting immunization regimen
    William O Rogers
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Infect Immun 70:4329-35. 2002
    ..These findings demonstrate that a multistage, multiantigen, DNA priming and poxvirus boosting vaccine regimen can protect nonhuman primates from an otherwise lethal malaria sporozoite challenge...
  14. pmc High-throughput generation of P. falciparum functional molecules by recombinational cloning
    João Carlos Aguiar
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Genome Res 14:2076-82. 2004
    ..falciparum genome and will provide for a resource for the malaria resource community distributed through public repositories...
  15. pmc Clinical trial in healthy malaria-naïve adults to evaluate the safety, tolerability, immunogenicity and efficacy of MuStDO5, a five-gene, sporozoite/hepatic stage Plasmodium falciparum DNA vaccine combined with escalating dose human GM-CSF DNA
    Thomas L Richie
    Naval Medical Research Center Silver Spring, MD, USA
    Hum Vaccin Immunother 8:1564-84. 2012
    ..It also laid the foundation for priming with DNA plasmids and boosting with recombinant viruses, an approach known for nearly 15 y to enhance the immunogenicity and protective efficacy of DNA vaccines...
  16. ncbi request reprint Anemia in parasite- and recombinant protein-immunized aotus monkeys infected with Plasmodium falciparum
    Trevor R Jones
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 66:672-9. 2002
    ..Partial immunity-whether induced by repeated immunization with whole parasites or with vaccine-seems important to the development of anemia...
  17. pmc Comparative genomics of the neglected human malaria parasite Plasmodium vivax
    Jane M Carlton
    The Institute for Genomic Research J Craig Venter Institute, 9704 Medical Research Drive, Rockville, Maryland 20850, USA
    Nature 455:757-63. 2008
    ..Completion of the P. vivax genome provides the scientific community with a valuable resource that can be used to advance investigation into this neglected species...
  18. ncbi request reprint Absence of antigenic competition in Aotus monkeys immunized with Plasmodium falciparum DNA vaccines delivered as a mixture
    Trevor R Jones
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Vaccine 20:1675-80. 2002
    ..The fourth immunization with the trivalent vaccine increased the mean antibody in IFAT by more than five-fold...
  19. doi request reprint The Effects of radiation on the safety and protective efficacy of an attenuated Plasmodium yoelii sporozoite malaria vaccine
    Rana Chattopadhyay
    Sanaria Inc, 9800 Medical Center Drive, Rockville, MD 20850, United States
    Vaccine 27:3675-80. 2009
    ..These results support the contention that a radiation attenuated sporozoite vaccine for malaria will be safe and effective at a range of radiation doses...
  20. pmc CpG oligodeoxynucleotide and Montanide ISA 51 adjuvant combination enhanced the protective efficacy of a subunit malaria vaccine
    Sanjai Kumar
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Infect Immun 72:949-57. 2004
    ..Our results indicate that this potent adjuvant formulation should be further evaluated for use in clinical trials of recombinant malarial vaccine candidates...
  21. ncbi request reprint Immunological responses of neonates and infants to DNA vaccines
    Martha Sedegah
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Methods Mol Med 127:239-51. 2006
    ..These data support assessment of this immunization strategy in neonates/young infants in areas where malaria exacts the greatest toll...
  22. ncbi request reprint Safety and clinical outcome of experimental challenge of human volunteers with Plasmodium falciparum-infected mosquitoes: an update
    Judith E Epstein
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, 20910, USA
    J Infect Dis 196:145-54. 2007
    ..In 1997, an analysis of experience with 118 volunteers indicated that mosquito inoculation of P. falciparum could be a safe, well-tolerated, reproducible, and efficient method of challenge...
  23. ncbi request reprint Nucleic acid vaccines against malaria
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Chem Immunol 80:308-21. 2002
  24. pmc Persistence of protective immunity to malaria induced by DNA priming and poxvirus boosting: characterization of effector and memory CD8(+)-T-cell populations
    Martha Sedegah
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
    Infect Immun 70:3493-9. 2002
    ..These results demonstrate that a heterologous priming and boosting vaccination can provide lasting protection against malaria in this model system...
  25. pmc BAFF and BAFF receptor levels correlate with B cell subset activation and redistribution in controlled human malaria infection
    Anja Scholzen
    Department of Medical Microbiology, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands
    J Immunol 192:3719-29. 2014
    ..These findings suggest an important role of BAFF in facilitating B cell subset proliferation and redistribution as a consequence of malaria-induced immune activation. ..
  26. ncbi request reprint Safety, tolerability, and antibody responses in humans after sequential immunization with a PfCSP DNA vaccine followed by the recombinant protein vaccine RTS,S/AS02A
    Judith E Epstein
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Vaccine 22:1592-603. 2004
    ....
  27. pmc Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Proc Natl Acad Sci U S A 100:9952-7. 2003
    ..The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens...
  28. pmc NF135.C10: a new Plasmodium falciparum clone for controlled human malaria infections
    Anne C Teirlinck
    Department of Medical Microbiology, Radboud University Nijmegen Medical Center, The Netherlands
    J Infect Dis 207:656-60. 2013
    ..C10 and 4 of 5 challenged with NF54 developed parasitemia as detected with microscopy. The 2 strains induced similar clinical signs and symptoms as well as cellular immunological responses...
  29. ncbi request reprint Parasitology. Malaria--from infants to genomics to vaccines
    Carole A Long
    Malaria Vaccine Development Unit, National Institutes of Health, Rockville, MD 20852, USA
    Science 297:345-7. 2002
  30. ncbi request reprint PfSPATR, a Plasmodium falciparum protein containing an altered thrombospondin type I repeat domain is expressed at several stages of the parasite life cycle and is the target of inhibitory antibodies
    Rana Chattopadhyay
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
    J Biol Chem 278:25977-81. 2003
    ..falciparum sporozoites specifically recognized the expression of this protein on transfected COS-7 cells. These data support the evaluation of this protein as a vaccine candidate...
  31. ncbi request reprint Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii
    Jane M Carlton
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:512-9. 2002
    ..This is the first genome sequence of a model eukaryotic parasite, and it provides insight into the use of such systems in the modelling of Plasmodium biology and disease...
  32. pmc Successful human infection with P. falciparum using three aseptic Anopheles stephensi mosquitoes: a new model for controlled human malaria infection
    Matthew B Laurens
    Howard Hughes Medical Institute Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 8:e68969. 2013
    ..If qPCR continues to be shown to be highly specific, and can be made to be practical, rapid, and standardized, it should be considered as an alternative for diagnosis...
  33. doi request reprint Plasmodium liver load following parenteral sporozoite administration in rodents
    Ivo H Ploemen
    Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Vaccine 31:3410-6. 2013
    ..Our findings provide a rationale for the design of clinical trials to optimize needle and syringe administration of Plasmodium falciparum sporozoites...
  34. pmc Plasmodium falciparum malaria challenge by the bite of aseptic Anopheles stephensi mosquitoes: results of a randomized infectivity trial
    Kirsten E Lyke
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 5:e13490. 2010
    ..The standard model relies on the bite of five infected mosquitoes to induce malaria. We examined the efficacy of malaria transmission using mosquitoes raised aseptically in compliance with current Good Manufacturing Practices (cGMPs)...
  35. pmc Gene disruption of Plasmodium falciparum p52 results in attenuation of malaria liver stage development in cultured primary human hepatocytes
    Ben C L van Schaijk
    Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 3:e3549. 2008
    ..falciparum and rodent malaria Plasmodium species generates parasites that become similarly arrested during liver stage development and these results pave the way for further development of GAS for human use...
  36. pmc Genome sequence of the human malaria parasite Plasmodium falciparum
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:498-511. 2002
    ..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
  37. ncbi request reprint Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:531-4. 2002
    ..We report here the nucleotide sequences of chromosomes 10, 11 and 14, and a re-analysis of the chromosome 2 sequence. These chromosomes represent about 35% of the 23-megabase P. falciparum genome...
  38. pmc Delineation of stage specific expression of Plasmodium falciparum EBA-175 by biologically functional region II monoclonal antibodies
    B Kim Lee Sim
    Protein Potential LLC, Rockville, Maryland, United States of America
    PLoS ONE 6:e18393. 2011
    ..Functional relationships between F1 and F2 domains and characterization of EBA-175 were studied using specific monoclonal antibodies (mAbs) against these domains...
  39. ncbi request reprint Safety, tolerability, and lack of antibody responses after administration of a PfCSP DNA malaria vaccine via needle or needle-free jet injection, and comparison of intramuscular and combination intramuscular/intradermal routes
    Judith E Epstein
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    Hum Gene Ther 13:1551-60. 2002
    ....
  40. ncbi request reprint Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites
    Stephen L Hoffman
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20850, USA
    J Infect Dis 185:1155-64. 2002
    ....
  41. ncbi request reprint A multilateral effort to develop DNA vaccines against falciparum malaria
    Sanjai Kumar
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    Trends Parasitol 18:129-35. 2002
    ....
  42. pmc Type II fatty acid biosynthesis is essential for Plasmodium falciparum sporozoite development in the midgut of Anopheles mosquitoes
    Ben C L van Schaijk
    Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Eukaryot Cell 13:550-9. 2014
    ..berghei and P. yoelii, where the FAS-II pathway is known to be required for normal parasite progression through the liver stage but is not required for oocyst development in the Anopheles mosquito midgut. ..
  43. ncbi request reprint Plasmodium, human and Anopheles genomics and malaria
    Stephen L Hoffman
    Celera Genomics, 45 West Gude Drive, Rockville, Maryland 20850, USA
    Nature 415:702-9. 2002
    ....
  44. ncbi request reprint The genome sequence of the malaria mosquito Anopheles gambiae
    Robert A Holt
    Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA
    Science 298:129-49. 2002
    ..An expressed sequence tag analysis of genes regulated by blood feeding provided insights into the physiological adaptations of a hematophagous insect...
  45. ncbi request reprint Laser capture microdissection and molecular analysis of Plasmodium yoelii liver-stage parasites
    John B Sacci
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Mol Biochem Parasitol 119:285-9. 2002
  46. ncbi request reprint Seasonal malaria attack rates in infants and young children in northern Ghana
    J Kevin Baird
    Naval Medical Research Center, Silver Spring, Maryland, USA
    Am J Trop Med Hyg 66:280-6. 2002
    ..These findings bear upon the design of malaria drug and vaccine trials in holoendemic areas...
  47. pmc Comparative immunopeptidomics of humans and their pathogens
    Sorin Istrail
    Celera Genomics, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 101:13268-72. 2004
    ....
  48. ncbi request reprint A DNA vaccine encoding the 42 kDa C-terminus of merozoite surface protein 1 of Plasmodium falciparum induces antibody, interferon-gamma and cytotoxic T cell responses in rhesus monkeys: immuno-stimulatory effects of granulocyte macrophage-colony stimulati
    Sanjai Kumar
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    Immunol Lett 81:13-24. 2002
    ..The GM-CSF plasmid or protein appears to be less potent as an adjuvant in rhesus monkeys than each is in mice, and more work is needed to determine if GM-CSF can be a useful adjuvant in DNA vaccination of primates...
  49. pmc Idiopathic acute myocarditis during treatment for controlled human malaria infection: a case report
    Maurits Pa van Meer
    Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands
    Malar J 13:38. 2014
    ..Definitive aetiology and pathophysiological mechanism for the myocarditis have not been established. ..
  50. doi request reprint Hark back: passive immunotherapy for influenza and other serious infections
    Thomas C Luke
    Henry Jackson Foundation, Naval Medical Research Center, Silver Spring, MD, USA
    Crit Care Med 38:e66-73. 2010
    ..Past, current, and future uses of immunotherapy and current advisory body recommendations for this approach are presented...

Research Grants8

  1. Potency of cryopreserved, irradiated sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2005
    ..The project will also provide a method for cryopreserving unirradiated sporozoites that could be used to challenge volunteers by injection with P. falciparum in vaccine studies worldwide. ..
  2. Genetically Attenuated Malaria Sporozote Vaccine
    Stephen Hoffman; Fiscal Year: 2007
    ..Success in this project has the potential to lead to development of a safe, effective, attenuated malaria vaccine. ..
  3. Attenuated Malaria Sporozoite Vaccine
    Stephen Hoffman; Fiscal Year: 2007
    ..Success in these first clinical trials will lead to a massive development effort leading to licensure and deployment of the vaccine. ..
  4. Lyophilization of Plasmodium falciparum sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2007
    ..Additionally, a dried vaccine may accelerate a concerted world-wide effort to eradicate this devastating disease. ..
  5. Universal Attenuated Malaria Sporozoite Vaccine and Challenge System
    Stephen Hoffman; Fiscal Year: 2007
    ..Success in this project will significantly decrease the cost of development and time to market for this malaria vaccine. ..