Niels Høiby

Summary

Publications

  1. pmc Investigation of the presence of human or bovine respiratory syncytial virus in the lungs of mink (Neovison vison) with hemorrhagic pneumonia due to Pseudomonas aeruginosa
    Charlotte M Salomonsen
    National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, DK 1870, Frederiksberg C, Denmark
    Acta Vet Scand 54:70. 2012
  2. pmc Bacterial adaptation during chronic infection revealed by independent component analysis of transcriptomic data
    Lei Yang
    Department of Systems Biology, Technical University of Denmark, DK 2800, Lyngby, Denmark
    BMC Microbiol 11:184. 2011
  3. doi request reprint Antibiotic resistance of bacterial biofilms
    Niels Høiby
    Department of Clinical Microbiology 9301, Juliane Mariesvej 22, Rigshospitalet, 2100 Copenhagen, Denmark
    Int J Antimicrob Agents 35:322-32. 2010
  4. doi request reprint Pseudomonas aeruginosa biofilms in cystic fibrosis
    Niels Høiby
    Department of Clinical Microbiology 9301, Rigshospitalet, University of Copenhagen, Juliane Maries Vej 22, Copenhagen, Denmark
    Future Microbiol 5:1663-74. 2010
  5. pmc The clinical impact of bacterial biofilms
    Niels Høiby
    Department of Clinical Microbiology, Rigshospitalet
    Int J Oral Sci 3:55-65. 2011
  6. pmc Recent advances in the treatment of Pseudomonas aeruginosa infections in cystic fibrosis
    Niels Høiby
    Department of Clinical Microbiology 9301, Rigshospitalet and ISIM, University of Copenhagen, Juliane Maries Vej 22, DK 2100 Copenhagen, Denmark
    BMC Med 9:32. 2011
  7. doi request reprint Augmented effect of early antibiotic treatment in mice with experimental lung infections due to sequentially adapted mucoid strains of Pseudomonas aeruginosa
    Maria Van Gennip
    Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
    J Antimicrob Chemother 64:1241-50. 2009
  8. doi request reprint Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
    Baoleri Lee
    Department of International Health, Immunology and Microbiology, Panum Institute, University of Copenhagen, Denmark
    APMIS 119:263-74. 2011
  9. pmc Novel experimental Pseudomonas aeruginosa lung infection model mimicking long-term host-pathogen interactions in cystic fibrosis
    Claus Moser
    Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    APMIS 117:95-107. 2009
  10. pmc Novel mouse model of chronic Pseudomonas aeruginosa lung infection mimicking cystic fibrosis
    Nadine Hoffmann
    Department of Bacteriology, Institute for Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Copenhagen DK 2200, Denmark
    Infect Immun 73:2504-14. 2005

Collaborators

Detail Information

Publications78

  1. pmc Investigation of the presence of human or bovine respiratory syncytial virus in the lungs of mink (Neovison vison) with hemorrhagic pneumonia due to Pseudomonas aeruginosa
    Charlotte M Salomonsen
    National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, DK 1870, Frederiksberg C, Denmark
    Acta Vet Scand 54:70. 2012
    ..Human respiratory syncytial virus (RSV) has been shown to augment infection with P. aeruginosa in mice and to promote adhesion of P. aeruginosa to human respiratory cells...
  2. pmc Bacterial adaptation during chronic infection revealed by independent component analysis of transcriptomic data
    Lei Yang
    Department of Systems Biology, Technical University of Denmark, DK 2800, Lyngby, Denmark
    BMC Microbiol 11:184. 2011
    ..However, exploitation of the complex, noisy and high-dimensional transcriptomic dataset is difficult and often hindered by low statistical power...
  3. doi request reprint Antibiotic resistance of bacterial biofilms
    Niels Høiby
    Department of Clinical Microbiology 9301, Juliane Mariesvej 22, Rigshospitalet, 2100 Copenhagen, Denmark
    Int J Antimicrob Agents 35:322-32. 2010
    ..A promising strategy may be the use of enzymes that can dissolve the biofilm matrix (e.g. DNase and alginate lyase) as well as quorum-sensing inhibitors that increase biofilm susceptibility to antibiotics...
  4. doi request reprint Pseudomonas aeruginosa biofilms in cystic fibrosis
    Niels Høiby
    Department of Clinical Microbiology 9301, Rigshospitalet, University of Copenhagen, Juliane Maries Vej 22, Copenhagen, Denmark
    Future Microbiol 5:1663-74. 2010
    ..aeruginosa biofilms. Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy, and they can be treated by chronic suppressive therapy...
  5. pmc The clinical impact of bacterial biofilms
    Niels Høiby
    Department of Clinical Microbiology, Rigshospitalet
    Int J Oral Sci 3:55-65. 2011
    ..Promising strategies may include the use of compounds which can dissolve the biofilm matrix and quorum sensing inhibitors, which increases biofilm susceptibility to antibiotics and phagocytosis...
  6. pmc Recent advances in the treatment of Pseudomonas aeruginosa infections in cystic fibrosis
    Niels Høiby
    Department of Clinical Microbiology 9301, Rigshospitalet and ISIM, University of Copenhagen, Juliane Maries Vej 22, DK 2100 Copenhagen, Denmark
    BMC Med 9:32. 2011
    ..aeruginosa infection did not improve lung function. Here I review the recent advances in the treatment of P. aeruginosa lung infections with a focus on inhalation treatments targeted at prophylaxis and chronic suppressive therapy...
  7. doi request reprint Augmented effect of early antibiotic treatment in mice with experimental lung infections due to sequentially adapted mucoid strains of Pseudomonas aeruginosa
    Maria Van Gennip
    Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
    J Antimicrob Chemother 64:1241-50. 2009
    ..The model reflects the differentiated behaviour of Pseudomonas aeruginosa mucoid strains isolated from the lungs of one chronically infected cystic fibrosis (CF) patient at different time periods during chronic lung infection...
  8. doi request reprint Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
    Baoleri Lee
    Department of International Health, Immunology and Microbiology, Panum Institute, University of Copenhagen, Denmark
    APMIS 119:263-74. 2011
    ..aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment...
  9. pmc Novel experimental Pseudomonas aeruginosa lung infection model mimicking long-term host-pathogen interactions in cystic fibrosis
    Claus Moser
    Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    APMIS 117:95-107. 2009
    ..In conclusion, adaptation of P. aeruginosa in CF is reflected by changed ability to establish lung infection and results in distinct host responses to mucoid and non-mucoid phenotypes...
  10. pmc Novel mouse model of chronic Pseudomonas aeruginosa lung infection mimicking cystic fibrosis
    Nadine Hoffmann
    Department of Bacteriology, Institute for Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Copenhagen DK 2200, Denmark
    Infect Immun 73:2504-14. 2005
    ..Therefore, the mouse model provides an improved method for evaluating the interaction between mucoid P. aeruginosa, the host, and antibacterial therapy...
  11. ncbi request reprint Epidemic spread of Pandoraea apista, a new pathogen causing severe lung disease in cystic fibrosis patients
    Inger Merete Jørgensen
    Department of Paediatrics, Danish Cystic Fibrosis Center, Rigshospitalet, Copenhagen Ø, Denmark
    Pediatr Pulmonol 36:439-46. 2003
    ..P. apista must therefore be considered a new and sometimes important pathogen for CF patients. Cohort isolation prevented further spread of P. apista in our CF center...
  12. pmc Azithromycin blocks quorum sensing and alginate polymer formation and increases the sensitivity to serum and stationary-growth-phase killing of Pseudomonas aeruginosa and attenuates chronic P. aeruginosa lung infection in Cftr(-/-) mice
    Nadine Hoffmann
    Department of Clinical Microbiology, Rigshospitalet, Institute for Medical Microbiology and Immunology, Panum Institute 24 1, University of Copenhagen, DK 2100, Copenhagen, Denmark
    Antimicrob Agents Chemother 51:3677-87. 2007
    ..aeruginosa, impairs its ability to form fully polymerized alginate biofilms, and increases its sensitivity to complement and stationary-phase killing, which may explain the clinical efficacy of AZM...
  13. pmc In vivo pharmacokinetics/pharmacodynamics of colistin and imipenem in Pseudomonas aeruginosa biofilm infection
    Wang Hengzhuang
    Department of Clinical Microbiology, Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark
    Antimicrob Agents Chemother 56:2683-90. 2012
    ..89) than planktonic cell infections (R(2) = 0.38). The postantibiotic effect (PAE) of colistin and imipenem was shorter in biofilm infections than planktonic cell infections in this model...
  14. pmc Ajoene, a sulfur-rich molecule from garlic, inhibits genes controlled by quorum sensing
    Tim Holm Jakobsen
    Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    Antimicrob Agents Chemother 56:2314-25. 2012
    ..aeruginosa was detected in ajoene-treated mice compared to a nontreated control group. This study adds to the list of examples demonstrating the potential of QS-interfering compounds in the treatment of bacterial infections...
  15. pmc High β-lactamase levels change the pharmacodynamics of β-lactam antibiotics in Pseudomonas aeruginosa biofilms
    Wang Hengzhuang
    Department of Clinical Microbiology, Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark
    Antimicrob Agents Chemother 57:196-204. 2013
    ..The inoculum effect of ceftazidime for the β-lactamase-overproducing mutant PAΔDDh2Dh3 biofilms was more obvious than for PAO1 biofilms, with a requirement of higher antibiotic concentration and a longer period of treatment...
  16. pmc Interactions between polymorphonuclear leukocytes and Pseudomonas aeruginosa biofilms on silicone implants in vivo
    Maria Van Gennip
    Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    Infect Immun 80:2601-7. 2012
    ..Here we present a novel method to study a pathogen-host interaction in detail. The data presented provide the first direct, high-resolution visualization of the failure of PMNs to protect against bacterial biofilms...
  17. pmc Food as a source for quorum sensing inhibitors: iberin from horseradish revealed as a quorum sensing inhibitor of Pseudomonas aeruginosa
    Tim Holm Jakobsen
    Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    Appl Environ Microbiol 78:2410-21. 2012
    ..Real-time PCR (RT-PCR) and DNA microarray studies showed that iberin specifically blocks expression of QS-regulated genes in P. aeruginosa...
  18. doi request reprint Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients
    Thomas Bjarnsholt
    Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Denmark
    Pediatr Pulmonol 44:547-58. 2009
    ..This strongly suggests that the conductive zone serves as a bacterial reservoir where the bacteria are organized in mucoid biofilms within the mucus, protected against antibiotics and host defenses...
  19. pmc Pseudomonas aeruginosa biofilms exposed to imipenem exhibit changes in global gene expression and beta-lactamase and alginate production
    Niels Bagge
    Department of Clinical Microbiology, Rigshospitalet, and Department of Bacteriology, Institute for Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Denmark
    Antimicrob Agents Chemother 48:1175-87. 2004
    ..g., an increased biofilm volume. Increased levels of alginate production may be an unintended adverse consequence of imipenem treatment in CF patients...
  20. doi request reprint Pseudomonas aeruginosa biofilm aggravates skin inflammatory response in BALB/c mice in a novel chronic wound model
    Hannah Trøstrup
    Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    Wound Repair Regen 21:292-9. 2013
    ..The results showed an aggravating impact of local inflammation induced by PA biofilms. In conclusion, our findings indicate that improved infection control of chronic wounds reduces the inflammatory response and may improve healing...
  21. ncbi request reprint Rapid necrotic killing of polymorphonuclear leukocytes is caused by quorum-sensing-controlled production of rhamnolipid by Pseudomonas aeruginosa
    Peter Ø Jensen
    Department of Clinical Microbiology, Rigshospitalet, DK 2100 Copenhagen Ø, Denmark
    Microbiology 153:1329-38. 2007
    ..aeruginosa by disabling the PMNs, which are a major first line of defence of the host. Furthermore, the study emphasizes the inhibition of QS as a target for the treatment of infections with P. aeruginosa...
  22. pmc Quorum sensing and virulence of Pseudomonas aeruginosa during lung infection of cystic fibrosis patients
    Thomas Bjarnsholt
    Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 5:e10115. 2010
    ..As seen from the present data, we can conclude that P. aeruginosa and particularly the mucoid strains do not lose the QS regulation or the ability to produce rhamnolipids until the late stage of the chronic infection...
  23. doi request reprint Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation
    Hong Wu
    Department of Clinical Microbiology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    FEMS Immunol Med Microbiol 62:49-56. 2011
    ..aeruginosa PAO1 by airway phagocytes, but did not affect phagocytosis of a PAO1-filM mutant. Our study suggests that ginseng treatment may help to eradicate the biofilm-associated chronic infections caused by P. aeruginosa...
  24. pmc Effects of intratracheal administration of novispirin G10 on a rat model of mucoid Pseudomonas aeruginosa lung infection
    Zhijun Song
    Department of Clinical Microbiology, Afsnit 9301, University Hospital of Copenhagen, Rigshospitalet, Juliane Maries Vej 22, DK 2100 Copenhagen Ø, Denmark
    Antimicrob Agents Chemother 49:3868-74. 2005
    ..0002). Lung cytokine responses in the treated group were significantly lower than in the control group. The results suggest that novispirin G10 might be useful in treating antibiotic-resistant P. aeruginosa lung infections...
  25. pmc Clearance of Pseudomonas aeruginosa foreign-body biofilm infections through reduction of the cyclic Di-GMP level in the bacteria
    Louise D Christensen
    Department of International Health, Immunology, and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
    Infect Immun 81:2705-13. 2013
    ..The present work provides proof of the concept that modulation of the c-di-GMP level in bacteria is a viable strategy for biofilm control. ..
  26. ncbi request reprint Serum concentrations of GM-CSF and G-CSF correlate with the Th1/Th2 cytokine response in cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection
    Claus Moser
    Department of Clinical Microbiology, Rigshospitalet, University Hospital, Copenhagen, Denmark
    APMIS 113:400-9. 2005
    ..The results indicate involvement of endogenous GM-CSF, G-CSF and IL-3 in the skewed Th response in CF, and change to a Th1-dominated response might be achieved with GM-CSF treatment...
  27. pmc Occurrence of hypermutable Pseudomonas aeruginosa in cystic fibrosis patients is associated with the oxidative stress caused by chronic lung inflammation
    Oana Ciofu
    Institute for Medical Microbiology and Immunology, University of Copenhagen, Panum Institute, IMMI 24 1, Blegdamsvej 3, 2200 Copenhagen N, Denmark
    Antimicrob Agents Chemother 49:2276-82. 2005
    ..The hypermutable phenotype can associate with mutations that confer adaptation of the bacteria in the lung and persistence of the infection...
  28. doi request reprint Colonisation and infection of the paranasal sinuses in cystic fibrosis patients is accompanied by a reduced PMN response
    Helle Krogh Johansen
    Department of Clinical Microbiology, Afsnit 9301, Rigshospitalet, Juliane Maries Vej 22, DK 2100 Copenhagen Ø, Denmark
    J Cyst Fibros 11:525-31. 2012
    ..We studied whether the sinuses might be foci for Pseudomonas aeruginosa lung infection...
  29. doi request reprint Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model
    Louise D Christensen
    Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, DK 2200 Copenhagen, Denmark
    J Antimicrob Chemother 67:1198-206. 2012
    ..We extended these results to an in vivo P. aeruginosa foreign-body biofilm model. The effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion...
  30. doi request reprint Poor antioxidant status exacerbates oxidative stress and inflammatory response to Pseudomonas aeruginosa lung infection in guinea pigs
    Peter Ø Jensen
    Department of Clinical Microbiology, University Hospital, Copenhagen, Denmark
    Basic Clin Pharmacol Toxicol 110:353-8. 2012
    ..Our data show that poor antioxidant status exacerbates the outcome of biofilm-related infections...
  31. pmc Phenotypes of non-attached Pseudomonas aeruginosa aggregates resemble surface attached biofilm
    Morten Alhede
    Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 6:e27943. 2011
    ....
  32. pmc Inactivation of the rhlA gene in Pseudomonas aeruginosa prevents rhamnolipid production, disabling the protection against polymorphonuclear leukocytes
    Maria Van Gennip
    Institute of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    APMIS 117:537-46. 2009
    ..Conclusively, the results support our model that rhamnolipids are key protective agents of P. aeruginosa against PMNs...
  33. pmc Heterogeneity of biofilms formed by nonmucoid Pseudomonas aeruginosa isolates from patients with cystic fibrosis
    Baoleri Lee
    Department of Clinical Microbiology, Rigshospitalet, Institute for Medical Microbiology and Immunology, University of Copenhagen, DK 2100 Copenhagen, Denmark
    J Clin Microbiol 43:5247-55. 2005
    ..The results suggest that the adherent characteristic of in vitro biofilm development was not essential for the longitudinal survival of nonmucoid P. aeruginosa during chronic lung colonization...
  34. doi request reprint Spread of colistin resistant non-mucoid Pseudomonas aeruginosa among chronically infected Danish cystic fibrosis patients
    Helle Krogh Johansen
    Department of Clinical Microbiology, Dept 9301 and Danish Cystic fibrosis Centre, Dept 5003, Rigshospitalet, Copenhagen, Denmark
    J Cyst Fibros 7:391-7. 2008
    ..Colistin resistant Pseudomonas aeruginosa have rarely been reported in cystic fibrosis (CF) patients...
  35. doi request reprint Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants
    Oana Ciofu
    Institute of International Health, Immunology and Microbiology, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Microbiology 154:103-13. 2008
    ..In conclusion, most non-mucoid isolates from chronically infected CF patients are revertants and the mechanism of revertance is algT-independent in the CF lung...
  36. pmc Extracellular DNA shields against aminoglycosides in Pseudomonas aeruginosa biofilms
    Wen Chi Chiang
    Department of International Health, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
    Antimicrob Agents Chemother 57:2352-61. 2013
    ..aeruginosa biofilms but rather by a protective shield effect of the extracellular DNA...
  37. ncbi request reprint Ginseng modulates the immune response by induction of interleukin-12 production
    Maria Waldorff Larsen
    Department of Clinical Microbiology, University Hospital Rigshospitalet, afsnit 7602, Copenhagen, Denmark
    APMIS 112:369-73. 2004
    ..The enhanced IL-12 production could induce a stronger Th1 response, resulting in better protection against infection with a variety of pathogens...
  38. doi request reprint Phenotypes selected during chronic lung infection in cystic fibrosis patients: implications for the treatment of Pseudomonas aeruginosa biofilm infections
    Oana Ciofu
    Department of International Health, Immunology and Microbiology, Unit of Bacteriology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    FEMS Immunol Med Microbiol 65:215-25. 2012
    ..In conclusion, these data underline the importance of biofilm prevention strategies by early aggressive antibiotic prophylaxis or therapy before phenotypic diversification during chronic lung infection of patients with cystic fibrosis...
  39. ncbi request reprint Effects of quorum-sensing on immunoglobulin G responses in a rat model of chronic lung infection with Pseudomonas aeruginosa
    Hong Wu
    Department of Clinical Microbiology, Afsnit 9301, University Hospital of Copenhagen, Rigshospitalet, Juliane Maries Vej 22, 2100, Copenhagen, Denmark
    Microbes Infect 6:34-7. 2004
    ..The results indicate that quorum signals contribute to the persistence of the infection and influence the immune response...
  40. pmc Dynamics and spatial distribution of beta-lactamase expression in Pseudomonas aeruginosa biofilms
    Niels Bagge
    Department of Clinical Microbiology, Rigshospitalet, and Department of Bacteriology, Institute for Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Denmark
    Antimicrob Agents Chemother 48:1168-74. 2004
    ..Thus, our experiments show that P. aeruginosa cells growing in biofilms constitute a heterogeneous population unit which may create different antibiotic-selective environments for the bacteria in the biofilm...
  41. ncbi request reprint Understanding bacterial biofilms in patients with cystic fibrosis: current and innovative approaches to potential therapies
    Niels Høiby
    Department of Clinical Microbiology and Danish Cystic Fibrosis Centre in Copenhagen, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
    J Cyst Fibros 1:249-54. 2002
    ..The new knowledge of the mechanisms involved in biofilm formation opens up new possibilities for therapeutic intervention strategies involving e.g. inhibitors of quorum sensing...
  42. doi request reprint Targeting quorum sensing in Pseudomonas aeruginosa biofilms: current and emerging inhibitors
    Tim Holm Jakobsen
    Costerton Biofilm Center, Department of International Health, Immunology and Microbiology, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Future Microbiol 8:901-21. 2013
    ....
  43. doi request reprint Respiratory bacterial infections in cystic fibrosis
    Oana Ciofu
    Institute of International Health, Immunology and Microbiology, Unit of Bacteriology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
    Curr Opin Pulm Med 19:251-8. 2013
    ..The purpose of this review is to summarize the knowledge on disease and treatment of infection with CF-related pathogens...
  44. ncbi request reprint Microbial pathogenesis and biofilm development
    Andreas Reisner
    BioCentrum DTU, Technical University of Denmark, Lyngbyt, Copenhagen, Denmark
    Contrib Microbiol 12:114-31. 2005
  45. doi request reprint Applying insights from biofilm biology to drug development - can a new approach be developed?
    Thomas Bjarnsholt
    1 Department of International Health, Immunology and Microbiology, Costerton Biofilm Centre Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark 2 Department of Clinical Microbiology, Rigshospitalet, 2100 Copenhagen Ø, Denmark
    Nat Rev Drug Discov 12:791-808. 2013
    ....
  46. ncbi request reprint Antibodies against beta-lactamase can improve ceftazidime treatment of lung infection with beta-lactam-resistant Pseudomonas aeruginosa in a rat model of chronic lung infection
    Oana Ciofu
    Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
    APMIS 110:881-91. 2002
    ..Our study showed that a beta ab with beta-lactamase inhibitory activity raised by immunization with beta-lactamase can improve the outcome of treatment with ceftazidime of resistant P. aeruginosa in a rat model of chronic lung infection...
  47. doi request reprint Genetic adaptation of Pseudomonas aeruginosa during chronic lung infection of patients with cystic fibrosis: strong and weak mutators with heterogeneous genetic backgrounds emerge in mucA and/or lasR mutants
    Oana Ciofu
    Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Denmark
    Microbiology 156:1108-19. 2010
    ..Similar evolutionary pathways concordant with adaptive radiation were observed in different clonal lineages of P. aeruginosa from CF patients...
  48. ncbi request reprint Cytokine modulating effect of ginseng treatment in a mouse model of Pseudomonas aeruginosa lung infection
    Zhijun Song
    Department of Clinical Microbiology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    J Cyst Fibros 2:112-9. 2003
    ..We therefore believe that Th1 response might benefit the host with P. aeruginosa lung infection and ginseng treatment might be a promising alternative measure for the treatment of chronic P. aeruginosa lung infection in CF patients...
  49. doi request reprint Nitrous Oxide Production in Sputum from Cystic Fibrosis Patients with Chronic Pseudomonas aeruginosa Lung Infection
    Mette Kolpen
    Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark Department of International health, Immunology and Microbiology, Faculty of Health Sciences University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 9:e84353. 2014
    ..Such ability for anaerobic growth may be a hitherto ignored key aspect of chronic P. aeruginosa infections that can inform new strategies for treatment and prevention. ..
  50. pmc Distribution, organization, and ecology of bacteria in chronic wounds
    Klaus Kirketerp-Møller
    Copenhagen Wound Healing Center, Bispebjerg Hospital, Copenhagen, Denmark
    J Clin Microbiol 46:2717-22. 2008
    ..This strongly supports the development of new diagnostic and treatment strategies for chronic wounds...
  51. doi request reprint A cohort study of the Copenhagen CF Centre eradication strategy against Staphylococcus aureus in patients with CF
    Christina Schjellerup Dalbøge
    Department of Clinical Microbiology, Copenhagen Ø, Denmark
    J Cyst Fibros 12:42-8. 2013
    ..Staphylococcus aureus is an important pathogen in CF. Centre prevalence of intermittent colonization and chronic S. aureus infections and the effectiveness of an anti-S. aureus eradication strategy was assessed...
  52. doi request reprint Development of antibiotic resistance and up-regulation of the antimutator gene pfpI in mutator Pseudomonas aeruginosa due to inactivation of two DNA oxidative repair genes (mutY, mutM)
    Lotte Frigaard Mandsberg
    Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    FEMS Microbiol Lett 324:28-37. 2011
    ..In conclusion, we showed that MutY and MutM are cooperating in the GO of P. aeruginosa, and that oxidative DNA lesions might represent an oxidative stress for the bacteria...
  53. pmc Pharmacokinetics/pharmacodynamics of colistin and imipenem on mucoid and nonmucoid Pseudomonas aeruginosa biofilms
    Wang Hengzhuang
    Department of Clinical Microbiology, Rigshospitalet, University Hospital of Copenhagen, Juliane Maries Vej 22, DK 2100 Copenhagen, Denmark
    Antimicrob Agents Chemother 55:4469-74. 2011
    ..Biofilms of mucoid P. aeruginosa were more difficult to eradicate than nonmucoid biofilms...
  54. pmc Lipocalin 2 is protective against E. coli pneumonia
    Hong Wu
    Granulocyte Research Laboratory, Rigshospitalet, Copenhagen, Denmark
    Respir Res 11:96. 2010
    ..coli is rare despite a frequent exposure to this commensal bacterium. Lipocalin 2 is an effector molecule of the innate immune system and could therefore play a role in hindering growth of E. coli in the lungs...
  55. ncbi request reprint Long-term azitromycin treatment of cystic fibrosis patients with chronic Pseudomonas aeruginosa infection; an observational cohort study
    Christine Rønne Hansen
    Cystic Fibrosis Center, 5003, Department of Pediatrics, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK 2100 København Ø, Denmark
    J Cyst Fibros 4:35-40. 2005
    ..In cystic fibrosis (CF), chronic endobronchial infection with Pseudomonas aeruginosa is a serious complication. Macrolides can increase lung function and weight in patients, and reduce exacerbations...
  56. ncbi request reprint Cytokine and surface receptor diversity of NK cells in resistant C3H/HeN and susceptible BALB/c mice with chronic Pseudomonas aeruginosa lung infection
    Henrik Calum
    Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
    APMIS 111:891-7. 2003
    ..In conclusion, the present results show phenotypic differences in NK cells in the two mice strains in chronic P. aeruginosa lung infection, indicating different modulating effects in the Th1/Th2 balance...
  57. doi request reprint Methods to classify bacterial pathogens in cystic fibrosis
    Thomas Bjarnsholt
    Department of Clinical Microbiology, University of Copenhagen, 2100 Copenhagen, Denmark
    Methods Mol Biol 742:143-71. 2011
    ..A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients...
  58. doi request reprint Diagnostic significance of measurements of specific IgG antibodies to Pseudomonas aeruginosa by three different serological methods
    Tacjana Pressler
    Department of Pediatrics, Rigshospitalet, University of Copenhagen, Denmark
    J Cyst Fibros 8:37-42. 2009
    ..The aim of the study was to evaluate three serological methods for their ability to identify CF patients in different infection status especially those at risk of developing chronic Pseudomonas aeruginosa (Pa) infection...
  59. doi request reprint Interference of Pseudomonas aeruginosa signalling and biofilm formation for infection control
    Thomas Bjarnsholt
    Department of International Health, Immunology and Microbiology, University of Copenhagen, 2200 Copenhagen, Denmark
    Expert Rev Mol Med 12:e11. 2010
    ..aeruginosa in vitro. A major task for the future is to investigate how such in vitro data correlate with the in vivo behaviour of P. aeruginosa, and how to treat chronic infections of this bacterium in patients...
  60. doi request reprint P. aeruginosa in the paranasal sinuses and transplanted lungs have similar adaptive mutations as isolates from chronically infected CF lungs
    Oana Ciofu
    Department of International Health, Immunology and Microbiology, Unit of Bacteriology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark Electronic address
    J Cyst Fibros 12:729-36. 2013
    ..Since different inflammatory responses and selective antibiotic pressures are acting in the sinuses compared with the lungs, we compared the adaptive profiles of mucoid and non-mucoid isolates from the two locations...
  61. doi request reprint Secretory IgA as a diagnostic tool for Pseudomonas aeruginosa respiratory colonization
    Kasper Aanaes
    Department of Otolaryngology Head and Neck Surgery and Audiology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Copenhagen, Denmark
    J Cyst Fibros 12:81-7. 2013
    ..Pseudomonas aeruginosa sinusitis may be the focus for intermittent lung colonization in patients with cystic fibrosis (CF). The sinusitis may induce elevated IgA levels in nasal secretion and saliva against P. aeruginosa...
  62. ncbi request reprint New antimicrobials in the management of cystic fibrosis
    Niels Høiby
    Department of Clinical Microbiology and Danish Cystic Fibrosis Centre, Rigshospitalet, University of Copenhagen, Blegdamsvej, Copenhagen, Denmark
    J Antimicrob Chemother 49:235-8. 2002
  63. doi request reprint Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition
    Morten Alhede
    Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    FEMS Immunol Med Microbiol 65:335-42. 2012
    ..Applying these methods to biofilms of Pseudomonas aeruginosa showed us that the dehydration of biofilms substantially influences its appearance and that a more authentic biofilm image emerges when combining all methods...
  64. doi request reprint Decreased mucosal oxygen tension in the maxillary sinuses in patients with cystic fibrosis
    Kasper Aanaes
    Department of Otolaryngology Head and Neck Surgery, Rigshospitalet, Denmark Blegdamsvej 9, DK 2100 Copenhagen, Denmark
    J Cyst Fibros 10:114-20. 2011
    ..Anoxic areas are found in the lower respiratory airways but it is unknown if the same conditions exist in the sinuses...
  65. doi request reprint Biofilms in chronic infections - a matter of opportunity - monospecies biofilms in multispecies infections
    Mette Burmølle
    Department of Biology, University of Copenhagen, Denmark
    FEMS Immunol Med Microbiol 59:324-36. 2010
    ..We discuss these differences from both the diagnostic and the scientific point of view...
  66. ncbi request reprint OXA-type carbapenemases
    Jan Walther-Rasmussen
    Department of Clinical Microbiology, 9301, Rigshospitalet, The National University Hospital, Copenhagen, Denmark
    J Antimicrob Chemother 57:373-83. 2006
    ..This article reviews the phylogeny and the genetic environments of the encoding genes and kinetic properties of the OXA-type carbapenemases...
  67. ncbi request reprint Antibody response to Pseudomonas aeruginosa in cystic fibrosis patients: a marker of therapeutic success?--A 30-year cohort study of survival in Danish CF patients after onset of chronic P. aeruginosa lung infection
    Helle Krogh Johansen
    Department of Clinical Microbiology, H S Rigshospitalet, Copenhagen, Denmark
    Pediatr Pulmonol 37:427-32. 2004
    ..aeruginosa lung infection improved with time (P = 0.008). Our study shows that CF patients who are treated intensively have lower antibody responses and longer survival after acquisition of chronic P. aeruginosa lung infection...
  68. ncbi request reprint Class A carbapenemases
    Jan Walther-Rasmussen
    Department of Clinical Microbiology, 9301, Rigshospitalet, National University Hospital, Copenhagen, Denmark
    J Antimicrob Chemother 60:470-82. 2007
    ..Class A carbapenemases are inhibited by clavulanate and tazobactam like other class A beta-lactamases...
  69. doi request reprint The in vivo biofilm
    Thomas Bjarnsholt
    Department of International Health, Immunology, and Microbiology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark Department of Clinical Microbiology 9301, Juliane Mariesvej 22, Copenhagen University Hospital, Copenhagen, Denmark Electronic address
    Trends Microbiol 21:466-74. 2013
    ..In this review, we discuss why the current in vitro models of biofilms might be limited for describing infectious biofilms, and we suggest new strategies for improving this discrepancy...
  70. pmc Sublethal ciprofloxacin treatment leads to rapid development of high-level ciprofloxacin resistance during long-term experimental evolution of Pseudomonas aeruginosa
    Karin Meinike Jørgensen
    Department of International Health, Immunology and Microbiology, Unit of Bacteriology, Costerton Biofilm Centre, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Antimicrob Agents Chemother 57:4215-21. 2013
    ..This can have implications for the long-term persistence of resistant bacteria and spread of antibiotic resistance by exposure of commensal bacterial flora to low antibiotic concentrations. ..
  71. pmc Unusual distribution of Burkholderia cepacia complex species in Danish cystic fibrosis clinics may stem from restricted transmission between patients
    Niels Nørskov-Lauritsen
    Department of Clinical Microbiology, Aarhus University Hospital, Skejby, DK 8200 Aarhus N, Denmark
    J Clin Microbiol 48:2981-3. 2010
    ..Although cases of cross infections were demonstrated, no major epidemic clone was found. The species distribution may represent the sporadic acquisition of bacteria from the environment...
  72. pmc Dietzia papillomatosis bacteremia
    Paul Rammer
    Department of Clinical Microbiology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Clin Microbiol 51:1977-8. 2013
    ..We present a case of infection with D. papillomatosis in a 2-year-old boy with known syringomyelia. The microbiological diagnosis was done using 16S rRNA gene sequencing. This is the first report of bacteremia with D. papillomatosis...
  73. doi request reprint A non-fatal case of invasive zygomycete (Lichtheimia corymbifera) infection in an allogeneic haematopoietic cell transplant recipient
    Steffen Eickhardt
    Department of International Health, Immunology and Microbiology, The Faculty of Health Sciences, The University of Copenhagen, Copenhagen, Denmark
    APMIS 121:456-9. 2013
    ..We herein describe a non-fatal case of Lichtheimia corymbifera infection in an allo-HCT recipient...
  74. pmc True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis
    Vibeke B Rudkjøbing
    Department of Biotechnology, Chemistry, and Environmental Engineering, Faculty of Engineering and Science, Aalborg University, Aalborg, Denmark
    J Clin Microbiol 49:4352-5. 2011
    ..A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens...
  75. ncbi request reprint Chronic infection with Achromobacter xylosoxidans in cystic fibrosis patients; a retrospective case control study
    Christine Rønne Hansen
    The Copenhagen Cystic Fibrosis Center, Departments of Paediatrics, Clinical Microbiology and Radiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    J Cyst Fibros 5:245-51. 2006
    ..In cystic fibrosis (CF), chronic infection of the airways with Achromobacter xylosoxidans have become more frequent. The pathogenic role of this is yet unclear...
  76. pmc Constitutive high expression of chromosomal beta-lactamase in Pseudomonas aeruginosa caused by a new insertion sequence (IS1669) located in ampD
    Niels Bagge
    Institute of Medical Microbiology and Immunology, Panum Institute, University of Copenhagen, Denmark
    Antimicrob Agents Chemother 46:3406-11. 2002
    ..Our results suggest that multiple pathways may exist leading to increased antimicrobial resistance due to chromosomal beta-lactamase...
  77. ncbi request reprint Cefotaximases (CTX-M-ases), an expanding family of extended-spectrum beta-lactamases
    Jan Walther-Rasmussen
    Deparment of Clinical Microbiology, The National University Hospital, Copenhagen, Denmark
    Can J Microbiol 50:137-65. 2004
    ..This review discusses the phylogeny and the hydrolytic properties of the CTX-M-ases, as well as their geographic occurrence and mode of spread...
  78. ncbi request reprint Prehospital diagnostic and therapeutic management of otogenic Streptococcus pneumoniae meningitis
    Christian Østergaard
    National Centre for Antimicrobials and Infection Control, Statens Serum Institute, Copenhagen, Denmark
    Scand J Infect Dis 38:172-80. 2006
    ..Moreover, OPM seems predominantly to occur in patients receiving no or inadequate antibiotic therapy for otitis media...