Johan W M Heemskerk

Summary

Publications

  1. doi request reprint Additive roles of platelets and fibrinogen in whole-blood fibrin clot formation upon dilution as assessed by thromboelastometry
    M Ninivaggi
    J W M Heemskerk, Department of Biochemistry CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands, Tel 31 43 3881671, Fax 31 43 3884159, E mail
    Thromb Haemost 111:447-57. 2014
  2. doi request reprint Platelet-based coagulation: different populations, different functions
    J W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 11:2-16. 2013
  3. doi request reprint Unravelling the different functions of protein kinase C isoforms in platelets
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    FEBS Lett 585:1711-6. 2011
  4. pmc Spatial distribution of factor Xa, thrombin, and fibrin(ogen) on thrombi at venous shear
    Michelle A Berny
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    PLoS ONE 5:e10415. 2010
  5. ncbi request reprint Dual role of platelet protein kinase C in thrombus formation: stimulation of pro-aggregatory and suppression of procoagulant activity in platelets
    Amrei Strehl
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biol Chem 282:7046-55. 2007
  6. ncbi request reprint Both ADP and thrombin regulate arteriolar thrombus stabilization and embolization, but are not involved in initial hemostasis as induced by micropuncture
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Microcirculation 14:193-205. 2007
  7. pmc Dual mechanism of integrin αIIbβ3 closure in procoagulant platelets
    Nadine J A Mattheij
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    J Biol Chem 288:13325-36. 2013
  8. ncbi request reprint Regulation of tissue factor-induced coagulation and platelet aggregation in flowing whole blood
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, University of Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 93:97-105. 2005
  9. pmc Roles of platelet STIM1 and Orai1 in glycoprotein VI- and thrombin-dependent procoagulant activity and thrombus formation
    Karen Gilio
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, 6200 MD Maastricht, The Netherlands
    J Biol Chem 285:23629-38. 2010
  10. pmc Non-redundant roles of phosphoinositide 3-kinase isoforms alpha and beta in glycoprotein VI-induced platelet signaling and thrombus formation
    Karen Gilio
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biol Chem 284:33750-62. 2009

Collaborators

Detail Information

Publications67

  1. doi request reprint Additive roles of platelets and fibrinogen in whole-blood fibrin clot formation upon dilution as assessed by thromboelastometry
    M Ninivaggi
    J W M Heemskerk, Department of Biochemistry CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands, Tel 31 43 3881671, Fax 31 43 3884159, E mail
    Thromb Haemost 111:447-57. 2014
    ..Clinical decisions for transfusion based on thromboelastometry should take into account the platelet concentration. ..
  2. doi request reprint Platelet-based coagulation: different populations, different functions
    J W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    J Thromb Haemost 11:2-16. 2013
    ..Furthermore, we discuss the ligands, platelet receptors and downstream intracellular signaling pathways implicated in these coagulant functions. These insights lead to an adapted model of platelet-based coagulation...
  3. doi request reprint Unravelling the different functions of protein kinase C isoforms in platelets
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    FEBS Lett 585:1711-6. 2011
    ..For identifying the substrate proteins in a living cell, new methodology is available and discussed...
  4. pmc Spatial distribution of factor Xa, thrombin, and fibrin(ogen) on thrombi at venous shear
    Michelle A Berny
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    PLoS ONE 5:e10415. 2010
    ..We sought to determine where activated factor X (FXa), (pro)thrombin, and fibrin(ogen) are localized in thrombi formed under venous shear...
  5. ncbi request reprint Dual role of platelet protein kinase C in thrombus formation: stimulation of pro-aggregatory and suppression of procoagulant activity in platelets
    Amrei Strehl
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biol Chem 282:7046-55. 2007
    ..This platelet signaling protein is the first one identified to balance the pro-aggregatory and procoagulant functions of thrombi...
  6. ncbi request reprint Both ADP and thrombin regulate arteriolar thrombus stabilization and embolization, but are not involved in initial hemostasis as induced by micropuncture
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Microcirculation 14:193-205. 2007
    ..Up to now, the relative importance of mediators involved is only partly known. It was the aim of this study to investigate the involvement of ADP and thrombin in subsequent phases of arteriolar hemostasis and thromboembolism in vivo...
  7. pmc Dual mechanism of integrin αIIbβ3 closure in procoagulant platelets
    Nadine J A Mattheij
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    J Biol Chem 288:13325-36. 2013
    ..Inactivation of integrin αIIbβ3 reverses platelet aggregate formation upon coagulation...
  8. ncbi request reprint Regulation of tissue factor-induced coagulation and platelet aggregation in flowing whole blood
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, University of Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 93:97-105. 2005
    ..These platelet inhibitors thus maintain their aggregation-inhibiting effect at sites of thrombin formation...
  9. pmc Roles of platelet STIM1 and Orai1 in glycoprotein VI- and thrombin-dependent procoagulant activity and thrombus formation
    Karen Gilio
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, 6200 MD Maastricht, The Netherlands
    J Biol Chem 285:23629-38. 2010
    ....
  10. pmc Non-redundant roles of phosphoinositide 3-kinase isoforms alpha and beta in glycoprotein VI-induced platelet signaling and thrombus formation
    Karen Gilio
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biol Chem 284:33750-62. 2009
    ..This provides a new mechanistic explanation for the anti-thrombotic effect of PI3K inhibition and makes PI3Kalpha an interesting new target for anti-platelet therapy...
  11. ncbi request reprint Dual P2Y 12 receptor signaling in thrombin-stimulated platelets--involvement of phosphoinositide 3-kinase beta but not gamma isoform in Ca2+ mobilization and procoagulant activity
    Paola E J van der Meijden
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, Maastricht, The Netherlands
    FEBS J 275:371-85. 2008
    ..Taken together, these results demonstrate a dual P2Y(12)-dependent signaling mechanism, which increases the platelet-activating effect of thrombin by prolongation of Ca(2+) elevation, thereby facilitating the coagulation process...
  12. ncbi request reprint The glycoprotein VI-phospholipase Cgamma2 signaling pathway controls thrombus formation induced by collagen and tissue factor in vitro and in vivo
    Imke C A Munnix
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 25:2673-8. 2005
    ..We investigated the signaling pathway of collagen-induced platelet activation in interaction with tissue factor-triggered coagulation during the thrombus-forming process...
  13. ncbi request reprint Synergistic effect of thrombin on collagen-induced platelet procoagulant activity is mediated through protease-activated receptor-1
    Jeffrey F W Keuren
    Sanquin, Blood Bank region South East, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 25:1499-505. 2005
    ..The objective of this study is to elucidate the mechanism of this synergistic action with a focus on the intracellular Ca2+ concentration ([Ca2+]i) and the various platelet receptors for thrombin...
  14. doi request reprint Key role of platelet procoagulant activity in tissue factor-and collagen-dependent thrombus formation in arterioles and venules in vivo differential sensitivity to thrombin inhibition
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Microcirculation 15:269-82. 2008
    ..We investigated the interplay of these processes in vivo in a mouse model of arteriolar and venular thrombus formation...
  15. doi request reprint Dual role of collagen in factor XII-dependent thrombus formation
    Paola E J van der Meijden
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Blood 114:881-90. 2009
    ....
  16. doi request reprint Acid sphingomyelinase regulates platelet cell membrane scrambling, secretion, and thrombus formation
    Patrick Münzer
    From the Departments of Physiology P M, O B, B W, E S, E M S, S S, S T T, C L, F L, Cardiology and Cardiovascular Medicine O B, M C, M E, P S, A E M, M G, and Dermatology M S, University of Tubingen, Germany Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands M A H F, J M E M C, J W M H Bristol Myers Squibb GmbH and CoKGaA, Munich, Germany K F Institute of Molecular Biology, University of Duisburg Essen, Essen, Germany E G and Department of Clinical and Experimental Hemostasis, Heinrich Heine University Dusseldorf, Dusseldorf, Germany M E
    Arterioscler Thromb Vasc Biol 34:61-71. 2014
    ..The present study thus explored whether acid sphingomyelinase participates in the regulation of platelet secretion, phosphatidylserine exposure, and thrombus formation...
  17. doi request reprint Both TMEM16F-dependent and TMEM16F-independent pathways contribute to phosphatidylserine exposure in platelet apoptosis and platelet activation
    Roger van Kruchten
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Blood 121:1850-7. 2013
    ....
  18. ncbi request reprint Principal role of glycoprotein VI in alpha2beta1 and alphaIIbbeta3 activation during collagen-induced thrombus formation
    Christelle Lecut
    Department of Biochemistry, CARIM, Maastricht University, The Netherlands
    Arterioscler Thromb Vasc Biol 24:1727-33. 2004
    ..High-shear perfusion of blood over collagen results in rapid platelet adhesion, aggregation, and procoagulant activity. We studied regulation of alpha2beta1 and alphaIIbbeta3 integrin activation during thrombus formation on collagen...
  19. ncbi request reprint Platelet P2Y12 receptors enhance signalling towards procoagulant activity and thrombin generation. A study with healthy subjects and patients at thrombotic risk
    Paola E J van der Meijden
    Department of Biochemistry, University of Maastricht, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 93:1128-36. 2005
    ..It is similarly active in platelets from control subjects and patients at thrombotic risk. Finally, the thrombogram method is useful for measuring incomplete P2Y(12) inhibition with clopidogrel...
  20. pmc Key role of integrin α(IIb)β (3) signaling to Syk kinase in tissue factor-induced thrombin generation
    Paola E J van der Meijden
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Cell Mol Life Sci 69:3481-92. 2012
    ..These results hence point to a novel role of Syk in integrin-dependent thrombin generation...
  21. ncbi request reprint Fibrillar type I collagens enhance platelet-dependent thrombin generation via glycoprotein VI with direct support of alpha2beta1 but not alphaIIbbeta3 integrin
    Christelle Lecut
    Department Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 94:107-14. 2005
    ....
  22. pmc Key role of glycoprotein Ib/V/IX and von Willebrand factor in platelet activation-dependent fibrin formation at low shear flow
    Judith M E M Cosemans
    Department of Biochemistry, Maastricht University MedicalCentre, Maastricht, The Netherlands
    Blood 117:651-60. 2011
    ..This process can be relevant in hemostasis and the manifestation of von Willebrand disease...
  23. pmc Platelet dysfunction in thrombosis patients treated with vitamin K antagonists and recurrent bleeding
    Paola E J van der Meijden
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University Medical Center, Maastricht, The Netherlands
    PLoS ONE 8:e64112. 2013
    ..In this proof-of-principle study, we investigated whether alterations in platelet function or von Willebrand factor (vWf) contribute to a bleeding phenotype in these patients...
  24. doi request reprint Antithrombotic potential of blockers of store-operated calcium channels in platelets
    Roger van Kruchten
    Department of Biochemistry and Cardiovascular Centre, Cardiovascular Research Institute Maastricht, University of Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 32:1717-23. 2012
    ..Pharmacological blockage of these channels may provide a novel way of antithrombotic therapy. Therefore, the thromboprotective effect of SOCE blockers directed against platelet Orai1 is determined...
  25. pmc Segregation of platelet aggregatory and procoagulant microdomains in thrombus formation: regulation by transient integrin activation
    Imke C A Munnix
    Department of Biochemistry, Maastricht University, CARIM, Maastricht University, The Netherlands
    Arterioscler Thromb Vasc Biol 27:2484-90. 2007
    ..Platelets play a dual role in thrombosis by forming aggregates and stimulating coagulation. We investigated the commitment of platelets to these separate functions during collagen-induced thrombus formation in vitro and in vivo...
  26. ncbi request reprint Role of murine integrin alpha2beta1 in thrombus stabilization and embolization: contribution of thromboxane A2
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    Thromb Haemost 98:1072-80. 2007
    ..The increased embolization in alpha2-null mice may argue against the use of alpha2beta1 integrin inhibitors for antithrombotic therapy...
  27. ncbi request reprint In vivo blockade of platelet ADP receptor P2Y12 reduces embolus and thrombus formation but not thrombus stability
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 23:518-23. 2003
    ..ADP is a key platelet agonist in thromboembolism. One of the receptors involved in ADP-induced platelet activation is the P2Y12 receptor, which is a target for antithrombotic drugs...
  28. doi request reprint Anticoagulant effect of dietary fish oil in hyperlipidemia: a study of hepatic gene expression in APOE2 knock-in mice
    Kristof Vanschoonbeek
    Department of Biochemistry, CARIM and NUTRIM, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 28:2023-9. 2008
    ..Using APOE2 knock-in mice, the relation of these fish-oil effects with altered gene expression was investigated...
  29. pmc Facilitating roles of murine platelet glycoprotein Ib and alphaIIbbeta3 in phosphatidylserine exposure during vWF-collagen-induced thrombus formation
    Marijke J E Kuijpers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
    J Physiol 558:403-15. 2004
    ..Hence, these glycoproteins have an assistant procoagulant role in collagen-dependent thrombus formation, which is most prominent at reduced GPVI activity and is independent of the presence of thrombin...
  30. ncbi request reprint Platelet ADP response deteriorates in synthetic storage media
    Jeffrey F W Keuren
    Sanquin Blood Bank Southeast Region, Maastricht, The Netherlands
    Transfusion 46:204-12. 2006
    ..The goal of this study was to evaluate the ADP-induced responses of PLTs stored in full plasma or in plasma and additive solution (AS)...
  31. ncbi request reprint Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, the Sanquin Blood Bank South East, the Department of Health Risk Analysis and Toxicology NUTRIM, Maastricht University, Maastricht, The Netherlands
    Transfusion 46:1018-28. 2006
    ..In vivo, this is antagonized by ectonucleotidases on the surface of endothelial cells and white blood cells (WBCs). The receptor desensitization of ATP- and ADP-induced responses of PLTs stored in plasma without WBCs was investigated...
  32. ncbi request reprint Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3
    Marion A H Feijge
    Department of Biochemistry, University of Maastricht, P O Box 616, Maastricht, MD 6200, The Netherlands
    Biochem Pharmacol 67:1559-67. 2004
    ..This crucial role of PDE3, but not of PDE5, extends to all major processes in thrombus formation: assembly of platelets into aggregates, secretion of autocrine products, and procoagulant activity...
  33. doi request reprint Role of newly formed platelets in thrombus formation in rat after clopidogrel treatment: comparison to the reversible binding P2Y₁₂ antagonist ticagrelor
    Marijke J E Kuijpers
    Dept of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 106:1179-88. 2011
    ..This difference is masked by conventional platelet aggregation methods, but is revealed by thrombus formation measurement under flow. Juvenile platelets formed at later time points after clopidogrel treatment promoted thrombus formation...
  34. pmc Stabilizing role of platelet P2Y(12) receptors in shear-dependent thrombus formation on ruptured plaques
    Reyhan Nergiz-Unal
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    PLoS ONE 5:e10130. 2010
    ..We investigated the role of P2Y(12) in thrombus formation on (collagen-containing) atherosclerotic plaques in vitro and in vivo, by using a novel mouse model of atherothrombosis...
  35. doi request reprint Effects of plasma dilution on tissue-factor-induced thrombin generation and thromboelastography: partly compensating role of platelets
    Saskia E M Schols
    Department of Biochemistry, Maastricht University and University Hospital, Maastricht, The Netherlands
    Transfusion 48:2384-94. 2008
    ..This treatment, however, can lead to dilutional coagulopathy and impaired hemostasis. We investigated the suitability of two integrative coagulation tests to measure the hemostatic activity of diluted plasma...
  36. ncbi request reprint Store-mediated calcium entry in the regulation of phosphatidylserine exposure in blood cells from Scott patients
    Imke C A Munnix
    Department of Biochemistry, CARIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands
    Thromb Haemost 89:687-95. 2003
    ..patient V.W. is a new case of Scott syndrome, ii. Ca(2+) entry in the platelets and lymphoblasts from both Scott patients is normal, and iii. elevated [Ca(2+)](i) as caused by SMCE is not sufficient to trigger PS exposure...
  37. ncbi request reprint Monitoring in vitro thrombus formation with novel microfluidic devices
    Erik Westein
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    Platelets 23:501-9. 2012
    ..This review highlights the progress that has been made in monitoring aspects of thrombus formation using microfluidic devices...
  38. ncbi request reprint Variable hypocoagulant effect of fish oil intake in humans: modulation of fibrinogen level and thrombin generation
    Kristof Vanschoonbeek
    Department of Human Biology, Nutrition and Toxicology Research and Cardiovascular Research Institutes of Maastricht, Maastricht University, Maastricht, The Netherlands
    Arterioscler Thromb Vasc Biol 24:1734-40. 2004
    ..We studied how fish oil intake influenced thrombin generation in plasma and which factors were involved herein...
  39. doi request reprint Platelet response heterogeneity in thrombus formation
    Imke C A Munnix
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Thromb Haemost 102:1149-56. 2009
    ..This recognition of thrombus heterogeneity has implications for the use and development of antiplatelet medication...
  40. ncbi request reprint Novel methodology for assessment of prophylactic platelet transfusion therapy by measuring increased thrombus formation and thrombin generation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, Maastricht University, Maastricht, The Netherlands
    Br J Haematol 136:480-90. 2007
    ..We conclude that assessment of flow-induced adhesion and thrombin generation in acquired thrombocytopenia adequately determines the improved haemostatic activity by transfused platelets...
  41. ncbi request reprint Shedding of procoagulant microparticles from unstimulated platelets by integrin-mediated destabilization of actin cytoskeleton
    Sandra Cauwenberghs
    Department of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    FEBS Lett 580:5313-20. 2006
    ..After transfusion of stored platelet preparations to thrombocytopenic patients, the microparticles contribute to coagulant activity in vivo...
  42. ncbi request reprint Integrin alphaIIbbeta3 and shear-dependent action of glycoprotein Ibalpha stimulate platelet-dependent thrombin formation in stirred plasma
    Jeffrey F W Keuren
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, University Maastricht, Maastricht, The Netherlands
    J Lab Clin Med 141:350-8. 2003
    ..We conclude that thrombin formation and coagulation in stirred PRP depend, to a large extent, on platelet adhesion to integrin alpha(IIb)beta(3) and, in a shear-dependent way, on GP Ib...
  43. ncbi request reprint Decreased responsiveness and development of activation markers of PLTs stored in plasma
    Joyce Curvers
    Sanquin Blood Bank Southeast Region, Maastricht, The Netherlands
    Transfusion 44:49-58. 2004
    ..PLTs collected for transfusion purposes preferably have retained these properties to restore impaired hemostasis with thrombocytopenia...
  44. ncbi request reprint Plasma triacylglycerol and coagulation factor concentrations predict the anticoagulant effect of dietary fish oil in overweight subjects
    Kristof Vanschoonbeek
    Department of Human Biology, Nutrition and Toxicology Research and Cardiovascular Research Institutes of Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    J Nutr 137:7-13. 2007
    ..We conclude that high fasting triacylglycerol concentrations predict high procoagulant activity and a lowering of thrombin potential with dietary fish oil...
  45. ncbi request reprint Hemostatic and signaling functions of transfused platelets
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, Maastricht University and University Hospital, The Netherlands
    Transfus Med Rev 21:287-94. 2007
    ....
  46. pmc Atherosclerotic geometries exacerbate pathological thrombus formation poststenosis in a von Willebrand factor-dependent manner
    Erik Westein
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    Proc Natl Acad Sci U S A 110:1357-62. 2013
    ..The developed stenotic microfluidic chamber offers a realistic platform for in vitro evaluation of shear-dependent thrombus formation in the setting of atherosclerosis...
  47. ncbi request reprint Continuous signaling via PI3K isoforms beta and gamma is required for platelet ADP receptor function in dynamic thrombus stabilization
    Judith M E M Cosemans
    Department of Biochemistry and Human Biology, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
    Blood 108:3045-52. 2006
    ..This novel concept of intrinsic, dynamic thrombus instability gives possibilities for the use of antiplatelet therapy...
  48. ncbi request reprint Measurement of the platelet procoagulant response
    Johan W M Heemskerk
    Department of Biochemistry, CARIM, Maastricht University, The Netherlands
    Methods Mol Biol 272:135-44. 2004
  49. ncbi request reprint Factor Xa and thrombin evoke additive calcium and proinflammatory responses in endothelial cells subjected to coagulation
    Valery Daubie
    Deparment of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands
    Biochim Biophys Acta 1763:860-9. 2006
    ..These data provide first evidence that these serine proteases trigger distinct signaling modules in endothelium that is activated by plasma coagulation...
  50. doi request reprint Atheroprotective effect of dietary walnut intake in ApoE-deficient mice: involvement of lipids and coagulation factors
    Reyhan Nergiz-Unal
    Dept of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, 6200 MD Maastricht, The Netherlands
    Thromb Res 131:411-7. 2013
    ..Consumption of n-3 polyunsaturated fatty acids (PUFA) and antioxidant polyphenols is considered to decline the risk of cardiovascular disease...
  51. doi request reprint Measurement of whole blood thrombus formation using parallel-plate flow chambers - a practical guide
    Roger van Kruchten
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Platelets 23:229-42. 2012
    ..Frequently encountered experimental problems and artifacts are discussed, as well as possibilities for using flow chamber devices as a diagnostic tool to test antithrombotic medication...
  52. ncbi request reprint Platelet collagen receptors and coagulation. A characteristic platelet response as possible target for antithrombotic treatment
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, University of Maastricht, The Netherlands
    Trends Cardiovasc Med 15:86-92. 2005
    ..The main signaling receptor for collagen, glycoprotein VI, plays a key role in the platelet procoagulant response during thrombus formation; this is a reason why new anti-glycoprotein-VI antibodies are promising antithrombotic tools...
  53. doi request reprint Increased thrombin generation and fibrinogen level after therapeutic plasma transfusion: relation to bleeding
    Saskia E M Schols
    Department of Biochemistry CARIM, Maastricht University, Maastricht, The Netherlands
    Thromb Haemost 99:64-70. 2008
    ..Furthermore, the data suggest that thrombin generation and fibrinogen are independent determinants of the risk of perioperative bleeding in this patient group...
  54. doi request reprint The effects of arterial flow on platelet activation, thrombus growth, and stabilization
    Judith M E M Cosemans
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, PO Box 616, Maastricht 6200 MD, The Netherlands
    Cardiovasc Res 99:342-52. 2013
    ..Analysis of the factors determining flow-dependent thrombus stabilization and embolus formation in mice will help to understand the regulation of this process in human arterial disease. ..
  55. ncbi request reprint Thrombin-induced hyperactivity of platelets of young stroke patients: involvement of thrombin receptors in the subject-dependent variability in Ca2+ signal generation
    Kristof Vanschoonbeek
    Dept of Human Biology, Cardiovascular Research Institute of Maastricht, University of Maastricht, Maastricht, The Netherlands
    Thromb Haemost 88:931-7. 2002
    ..Furthermore, the subject-dependent variation in thrombin-induced signalling is likely to involve PAR1-mediated platelet activation...
  56. doi request reprint Correction of coagulation in dilutional coagulopathy: use of kinetic and capacitive coagulation assays to improve hemostasis
    Saskia E M Schols
    Department of Biochemistry, CARIM, Maastricht University Medical Centre, Maastricht, The Netherlands
    Transfus Med Rev 24:44-52. 2010
    ..Although there is current consensus on the application of fresh frozen plasma to revert coagulopathy, factor concentrates may appear to be useful in the future...
  57. ncbi request reprint Complementary roles of glycoprotein VI and alpha2beta1 integrin in collagen-induced thrombus formation in flowing whole blood ex vivo
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    FASEB J 17:685-7. 2003
    ....
  58. ncbi request reprint Real-time detection of activation patterns in individual platelets during thromboembolism in vivo: differences between thrombus growth and embolus formation
    Miriam A van Gestel
    Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, NL 6200 MD Maastricht, The Netherlands
    J Vasc Res 39:534-43. 2002
    ..These results show, for the first time, the relation between single platelet activation patterns, which are different during thrombus growth and embolus formation, and their behavior in a thromboembolic process in vivo...
  59. doi request reprint Molecular MRI of early thrombus formation using a bimodal alpha2-antiplasmin-based contrast agent
    Robbert Jan J H M Miserus
    Department of Radiology, Maastricht University Medical Centre, Maastricht, The Netherlands
    JACC Cardiovasc Imaging 2:987-96. 2009
    ..We aimed to investigate whether early thrombus formation can be visualized with in vivo magnetic resonance imaging (MRI) by the use of a novel bimodal alpha(2)-antiplasmin-based contrast agent (CA)...
  60. doi request reprint Targeting platelet receptor function in thrombus formation: the risk of bleeding
    Frauke Swieringa
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    Blood Rev 28:9-21. 2014
    ..Recording of bleeding due to (congenital) platelet dysfunction or (acquired) antiplatelet treatment occurs according to different protocols, while common laboratory methods are used to determine platelet function. ..
  61. pmc Platelet CD40L mediates thrombotic and inflammatory processes in atherosclerosis
    Dirk Lievens
    Department of Pathology, Maastricht Center for Atherosclerosis Research, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Blood 116:4317-27. 2010
    ....
  62. ncbi request reprint The quercetin paradox
    Agnes W Boots
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands
    Toxicol Appl Pharmacol 222:89-96. 2007
    ..Apparently, the potential toxicity of metabolites formed during the actual antioxidant activity of free radical scavengers should be considered in antioxidant supplementation...
  63. ncbi request reprint Platelet activation and blood coagulation
    Johan W M Heemskerk
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, The Netherlands
    Thromb Haemost 88:186-93. 2002
    ..In addition, platelets can support the initiation phase of coagulation by providing binding sites for prothrombin and factor XI. They thereby take over the initiating role of tissue factor and factor VIIa in coagulation activation...
  64. ncbi request reprint Glutathione oxidation in calcium- and p38 MAPK-dependent membrane blebbing of endothelial cells
    Roosje M A van Gorp
    Department of Human Biology, Maastricht University, 6200 MD Maastricht, The Netherlands
    Biochim Biophys Acta 1591:129-138. 2002
    ....
  65. doi request reprint Intravital imaging of thrombus formation in small and large mouse arteries: experimentally induced vascular damage and plaque rupture in vivo
    Marijke J E Kuijpers
    Department of Biochemistry, CARIM, Maastricht University, Maastricht, The Netherlands
    Methods Mol Biol 788:3-19. 2012
    ..These approaches provide important information that cannot be obtained with ex vivo methods and thus are likely to lead to new insights into the complex process of thrombosis...
  66. ncbi request reprint Irregular spiking in free calcium concentration in single, human platelets. Regulation by modulation of the inositol trisphosphate receptors
    Roosje M A van Gorp
    Department of Biochemistry, University of Maastricht, The Netherlands
    Eur J Biochem 269:1543-52. 2002
    ..The observation that individual Ca2+ peaks were generated in various parts of a platelet at similar intervals and amplitudes points to effective cooperation of the various stores in the Ca2+-release process...
  67. ncbi request reprint Polyphosphates: a link between platelet activation, intrinsic coagulation and inflammation?
    Paola E J van der Meijden
    Department of Biochemistry, CARIM, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands p vandermeijden maastrichtuniversity nl
    Expert Rev Hematol 3:269-72. 2010
    ..This results in vascular leakage and edema formation in vivo. Together, polyP release from platelets has a physiological role in the thrombus-forming process, as well as in inflammatory reactions...